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    Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device
    81.
    发明授权
    Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device 有权
    用于核酸提取的离心力微流体装置和包括微流体装置的微流体系统

    公开(公告)号：US08273310B2

    公开(公告)日：2012-09-25

    申请号：US11848748

    申请日：2007-08-31

    申请人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

    发明人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

    IPC分类号： B04B13/00

    CPC分类号： B01L3/502738 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/0233 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0076 ,  F16K2099/0084

    摘要： A centrifugal force-based microfluidic device for nucleic acid extraction and a microfluidic system are provided. The microfluidic device includes a body of revolution; a microfluidic structure disposed in the body of revolution, the microfluidic structure including a plurality of chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structure transmitting the fluid using centrifugal force due to rotation of the body of revolution; and magnetic beads contained in one of the chambers which collect a target material from a biomaterial sample flowing into the chamber, wherein the microfluidic structure washes the magnetic beads which collect the target material, and separates nucleic acid by electromagnetic wave irradiation from an external energy source to the magnetic beads. The microfluidic system includes the microfluidic device; a rotation operating unit which rotates the body of revolution; and an external energy source which irradiates electromagnetic waves.

    摘要翻译： 提供了用于核酸提取的离心力微流体装置和微流体系统。 微流体装置包括一个革命体; 设置在旋转体内的微流体结构，所述微流体结构包括多个室，连接所述室的通道和设置在通道中的阀以控制流体流动，所述微流体结构由于所述体的旋转而使用离心力传递流体 的革命 以及包含在其中一个室中的磁珠，其从流入腔室的生物材料样品收集目标材料，其中微流体结构洗涤收集目标材料的磁珠，并通过来自外部能量源的电磁波照射分离核酸 到磁珠。 微流体系统包括微流体装置; 使旋转体旋转的旋转操作单元; 以及照射电磁波的外部能量源。

    DEVICE AND METHOD OF SEPARATING CELLS BY USING MAGNETIC FORCE
    84.
    发明申请
    DEVICE AND METHOD OF SEPARATING CELLS BY USING MAGNETIC FORCE 有权
    使用磁力分离细胞的装置和方法

    公开(公告)号：US20120077267A1

    公开(公告)日：2012-03-29

    申请号：US13051251

    申请日：2011-03-18

    申请人： Hun-joo LEE ,  Jeong-gun LEE ,  Mi-jeong SONG ,  Jong-myeon PARK ,  Tae-seok SIM ,  Min-seok S. KIM

    发明人： Hun-joo LEE ,  Jeong-gun LEE ,  Mi-jeong SONG ,  Jong-myeon PARK ,  Tae-seok SIM ,  Min-seok S. KIM

    IPC分类号： C12N5/00 ,  C12M1/00

    CPC分类号： C12M47/04 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0864 ,  B03C1/02 ,  B03C1/14 ,  B03C1/145 ,  C12M1/266 ,  G01N1/4077 ,  Y10T436/25

    摘要： A cell separation device includes a channel or chamber in which a sample flows or moves, the sample including target cells marked with magnetic particles, and non-target cells, and a magnet which generates a magnetic first force in a first direction with respect to the sample within the channel or chamber. The channel or chamber of the cell separation device is applied with a second force in a second direction opposite to the first direction of the magnetic force. According to the cell separation device and a method of separating cells, the target cells move in the first direction by the magnetic force, and the non-target cells move in the second direction by the second force, by simultaneously applying the magnetic force and the second force in opposing directions, thereby separating the target cells from the non-target cells.

    摘要翻译： 细胞分离装置包括样品流动或移动的通道或室，包括标记有磁性颗粒的靶细胞的样品和非靶细胞，以及相对于第一方向产生第一方向的磁性第一力的磁体 样品在通道或室内。 细胞分离装置的通道或腔室以与磁力的第一方向相反的第二方向施加第二力。 根据细胞分离装置和分离细胞的方法，靶细胞通过磁力在第一方向上移动，非靶细胞通过第二力在第二方向上移动，同时施加磁力和 从而将目标细胞与非靶细胞分离。

    Apparatus for and method of separating polarizable analyte using dielectrophoresis
    85.
    发明授权
    Apparatus for and method of separating polarizable analyte using dielectrophoresis 有权
    使用介电电泳分离可极化分析物的装置和方法

    公开(公告)号：US08137523B2

    公开(公告)日：2012-03-20

    申请号：US11754523

    申请日：2007-05-29

    申请人： Yoon-kyoung Cho ,  Su-hyeon Kim ,  Chin-sung Park ,  Kyu-sang Lee ,  Jeong-gun Lee

    发明人： Yoon-kyoung Cho ,  Su-hyeon Kim ,  Chin-sung Park ,  Kyu-sang Lee ,  Jeong-gun Lee

    IPC分类号： G01N27/26 ,  G01N27/447

    CPC分类号： B03C5/005 ,  B03C5/026 ,  B03C2201/26

    摘要： An apparatus separating a polarizable analyte using dielectrophoresis includes a vessel including a membrane having a plurality of nano- to micro-sized pores, the membrane disposed inside the vessel, electrodes generating spatially non-uniform electric fields in the nano- to micro-sized pores of the membrane when an AC voltage is applied to the electrodes, and a power source applying the AC voltage to the electrodes, wherein a sectional area of the pores varies along a depth of the pores. A method of separating a polarizable material uses the apparatus.

    摘要翻译： 使用介电电泳分离可极化分析物的装置包括容器，其包括具有多个纳米至微米尺寸孔的膜，所述膜设置在容器内部，电极在纳米至微孔中产生空间上不均匀的电场 当向电极施加AC电压时，将膜施加到膜上，并且向电极施加AC电压的电源，其中孔的截面积沿着孔的深度而变化。 分离可极化材料的方法使用该装置。

    Microfluidic device using microfluidic chip and microfluidic device using biomolecule microarray chip
    88.
    发明授权
    Microfluidic device using microfluidic chip and microfluidic device using biomolecule microarray chip 有权
    使用微流控芯片和微流控器件的微流控器件使用生物分子微阵列芯片

    公开(公告)号：US07988915B2

    公开(公告)日：2011-08-02

    申请号：US12115572

    申请日：2008-05-06

    申请人： Beom-seok Lee ,  Jeong-gun Lee ,  Jong-myeon Park ,  Yoon-kyoung Cho

    发明人： Beom-seok Lee ,  Jeong-gun Lee ,  Jong-myeon Park ,  Yoon-kyoung Cho

    IPC分类号： C40B60/12

    CPC分类号： B01L3/502738 ,  B01L3/502707 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0621 ,  B01L2200/16 ,  B01L2300/04 ,  B01L2300/0636 ,  B01L2300/0806 ,  B01L2400/0409 ,  B01L2400/0677 ,  Y10T436/15

    摘要： Disclosed is a microfluidic device including a microfluidic structure formed in a platform in which various examinations, such as an immune serum examination, can be automatically performed using the biomolecule microarray chip. The biomolecule microarray chip-type microfluidic device using a biomolecule microarray chip comprises: a platform which is rotatable; a microfluidic structure disposed in the platform, comprising: a plurality of chambers; a plurality of channels connecting the chambers each other; and a plurality of valves controlling flow of fluids through the channels, wherein the microfluidic structure controls flow of a fluid sample using rotation of the platform and the valves; and a biomolecule microarray chip mounted in the platform such that biomolecule capture probes bound to the biomolecule microarray chip contact the fluid sample in the microfluidic structure.

    摘要翻译： 公开了一种微流体装置，其包括在平台中形成的微流体结构，其中可以使用生物分子微阵列芯片自动执行各种检查，例如免疫血清检查。 使用生物分子微阵列芯片的生物分子微阵列芯片型微流器件包括：可旋转的平台; 设置在平台中的微流体结构，包括：多个室; 连接所述室的多个通道; 以及多个阀，其控制通过所述通道的流体流动，其中所述微流体结构通过所述平台和所述阀的旋转来控制流体样品的流动; 以及安装在平台中的生物分子微阵列芯片，使得与生物分子微阵列芯片结合的生物分子捕获探针与微流体结构中的流体样品接触。

    Method and apparatus for disrupting cells and amplifying nucleic acids using gold nanorods
    89.
    发明授权
    Method and apparatus for disrupting cells and amplifying nucleic acids using gold nanorods 有权
    使用金纳米棒破坏细胞和扩增核酸的方法和装置

    公开(公告)号：US07972819B2

    公开(公告)日：2011-07-05

    申请号：US11838550

    申请日：2007-08-14

    申请人： Kwang Ho Cheong ,  Dong-kee Yi ,  Jeong-gun Lee ,  Jong-myeon Park

    发明人： Kwang Ho Cheong ,  Dong-kee Yi ,  Jeong-gun Lee ,  Jong-myeon Park

    IPC分类号： C12P19/34 ,  C12N13/00 ,  C12N1/08

    CPC分类号： C12N1/066 ,  C12M47/06 ,  C12N13/00 ,  C12P19/34

    摘要： Provided is a method of disrupting cells comprising adding gold nanorods to a solution containing cells and irradiating the gold nanorods with a laser to disrupt the cells. A method and an apparatus for continuously disrupting cells and amplifying nucleic acids in a single microchamber are also provided, wherein the method comprises introducing a solution containing cells and gold nanorods into a microchamber, irradiating a laser onto the gold nanorods to disrupt the cells, and amplifying a nucleic acid from the disrupted cells in the microchamber.

    摘要翻译： 提供一种破坏细胞的方法，包括向含有细胞的溶液中加入金纳米棒并用激光照射金纳米棒以破坏细胞。 还提供了用于在单个微室中连续破坏细胞和扩增核酸的方法和装置，其中所述方法包括将含有细胞和金纳米棒的溶液引入微室中，将激光照射到金纳米棒上以破坏细胞，以及 在微室中从破碎的细胞中扩增核酸。

    Centrifugal force based microfluidic device for dilution and microfluidic system including the same
    90.
    发明授权
    Centrifugal force based microfluidic device for dilution and microfluidic system including the same 失效
    用于稀释和微流体系统的基于离心力的微流体装置包括相同的

    公开(公告)号：US07951332B2

    公开(公告)日：2011-05-31

    申请号：US12029480

    申请日：2008-02-12

    申请人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

    发明人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

    IPC分类号： B01L11/00

    CPC分类号： B01L3/502738 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677

    摘要： Provided are a centrifugal force based microfluidic device which can automatically perform a dilution operation and a microfluidic system including the same. The centrifugal force based microfluidic device for dilution includes a rotatable disk type platform, a mixing chamber disposed on the platform; a buffer solution storage disposed on a portion of the platform which is closer to a center of the platform than the mixing chamber, connected to the mixing chamber through a channel to supply a predetermined amount of buffer solution to the mixing chamber at least one time, and a plurality of diluted solution chambers which are disposed on a portion of the platform which is farther from the center of the platform than the mixing chamber, are each connected to the mixing chamber through flow paths extended from a middle exit corresponding to a predetermined water level, and sequentially receiving a solution which is serially diluted in the mixing chamber at least one time.

    摘要翻译： 提供了一种能够自动执行稀释操作的离心力微流体装置和包括其的微流体系统。 用于稀释的基于离心力的微流体装置包括可旋转盘式平台，设置在平台上的混合室; 缓冲溶液储存器，其设置在所述平台的比混合室更靠近所述平台的中心的部分上，所述缓冲溶液存储器经由通道至少一次向所述混合室提供预定量的缓冲溶液，所述通道连接到所述混合室， 并且多个稀释溶液室通过从对应于预定水的中间出口延伸的流路连接到平台的比混合室更远离平台中心的部分上的稀释溶液室 至少一次连续地接收在混合室中连续稀释的溶液。