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106 条记录 (耗时 0.04 秒)

    Cell culture systems and methods for organ assist devices
    81.
    发明授权
    Cell culture systems and methods for organ assist devices 有权

    公开(公告)号：US06465252B1

    公开(公告)日：2002-10-15

    申请号：US09599891

    申请日：2000-06-21

    申请人： Mehmet Toner ,  Arno W. Tilles ,  Ulysses J. Balis ,  Martin L. Yarmush ,  Maury D. Cosman ,  Paul A. Dimilla

    发明人： Mehmet Toner ,  Arno W. Tilles ,  Ulysses J. Balis ,  Martin L. Yarmush ,  Maury D. Cosman ,  Paul A. Dimilla

    IPC分类号： C12N500

    摘要： The invention features modular cell culturing devices including one or more flat-plate modules, and is based on the discovery that if the flows of liquid medium and oxygenated fluid are separated by a gas-permeable, liquid-impermeable membrane, and the cells are grown attached to the liquid side of the membrane, the device can be used to culture cells with transport of oxygen through the membrane (i.e., direct oxygenation), without regard for the flow rate of the liquid medium passing through the device. The new flow-through cell culturing devices can thus be used to culture cells, e.g., hepatocytes, with high levels of cell function in organ, e.g., liver, assist systems, for production of cells, for production of cell-derived products, such as, proteins or viruses, or for systems to treat biological liquids to remove toxins, such as, ammonia, or add cell-synthesized products, or both.

    Ultra rapid freezing for cell cryopreservation
    82.
    发明授权
    Ultra rapid freezing for cell cryopreservation 有权
    超快速冷冻细胞冷冻保存

    公开(公告)号：US06403376B1

    公开(公告)日：2002-06-11

    申请号：US09587778

    申请日：2000-06-06

    申请人： Mehmet Toner ,  Alex J. Fowler

    发明人： Mehmet Toner ,  Alex J. Fowler

    IPC分类号： A01N100

    CPC分类号： A01N1/02 ,  A01N1/0284 ,  C12N1/04

    摘要： A method for preserving biological material includes the steps of placing the biological material in thermal contact with a cryogenically coolable environment, cooling the surrounding environment to a temperature below the glass phase transition temperature of the biological material, applying radiant energy to the biological material to melt at least a portion of the biological material, and rapidly stopping the application of radiant energy to the biological material to rapidly cool and varify the melted portion of the biological material. The method produces cooling rates so rapid that the biological material is vitrified without an opportunity for ice crystals to form.

    摘要翻译： 保存生物材料的方法包括以下步骤：将生物材料与低温可冷却环境热接触，将周围环境冷却至低于生物材料的玻璃化转变温度的温度，向生物材料施加辐射能以熔化 至少一部分生物材料，并迅速停止向生物材料施加辐射能以快速冷却和变形生物材料的熔融部分。 该方法产生如此快速的冷却速率，使得生物材料被玻璃化，而没有形成冰晶的机会。

    Controlled reversible poration for preservation of biological materials
    84.
    发明授权
    Controlled reversible poration for preservation of biological materials 有权
    用于保存生物材料的可控可逆定点

    公开(公告)号：US6127177A

    公开(公告)日：2000-10-03

    申请号：US151821

    申请日：1998-09-11

    申请人： Mehmet Toner ,  Michael Russo ,  Robert Bieganski

    发明人： Mehmet Toner ,  Michael Russo ,  Robert Bieganski

    IPC分类号： A01N1/02 ,  C12N5/00

    CPC分类号： A01N1/0221 ,  A01N1/02 ,  Y10S977/902 ,  Y10S977/92 ,  Y10S977/944

    摘要： A preservation method for biological material having cell membranes includes reversibly porating the cell membranes; loading a bio-protective agent having bio-preservation properties to a predetermined intracellular concentration; preparing the bio-protective agent loaded biological material for storage; storing the biological material; recovering the stored biological material from storage; and reversing the cell membrane poration. H5 .alpha.-toxin, a genetically engineered mutant of Staphylococcus aureus .alpha.-hemolysin, may be used as a porating agent. Non-permeating sugars such as trehalose and sucrose may be used as the bio-protective agent.

    摘要翻译： 具有细胞膜的生物材料的保存方法包括可逆地将细胞膜打孔; 将具有生物保存性能的生物保护剂装载到预定的细胞内浓度; 制备装载生物保护剂的生物材料进行储存; 储存生物材料; 从储存中回收储存的生物材料; 并逆转细胞膜引诱。 H5α-毒素，金黄色葡萄球菌α-溶血素的遗传工程改造的突变体，可用作开孔剂。 可以使用非渗透性糖如海藻糖和蔗糖作为生物保护剂。

    Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
    85.
    发明授权
    Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing 失效
    通过慢速冷冻冷冻保存收获的皮肤和培养的皮肤或角膜等效物

    公开(公告)号：US5891617A

    公开(公告)日：1999-04-06

    申请号：US380099

    申请日：1995-01-30

    申请人： Stephen Watson ,  Mehmet Toner

    发明人： Stephen Watson ,  Mehmet Toner

    IPC分类号： F25D17/02 ,  A01N1/02 ,  B65D55/06 ,  B65D77/04 ,  B65D77/20 ,  B65D85/50

    CPC分类号： A01N1/02 ,  A01N1/0221 ,  A01N1/0263 ,  A01N1/0284 ,  B65D55/06 ,  B65D77/0486 ,  B65D85/50

    摘要： This invention is directed to cryopreservation of harvested mammalian tissues and living cultured tissue equivalents made by in vitro technology. The invention involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution, agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue, and then freezing the tissue at a very slow freezing rate at 0.3.degree. C. or less/min. In the freezing step, extracellular ice formation is initiated by seeding. The cryopreserved tissue may be stored for indefinite periods of time prior to use. The cultured tissue equivalent is an in vitro model of the equivalent human tissue, such as skin or cornea, which, when retrieved from storage can be used for transplantation or implantation in vivo or for screening compounds in vitro.

    摘要翻译： 本发明涉及通过体外技术制备的收获的哺乳动物组织和活的培养组织等同物的低温保存。 本发明涉及将哺乳动物组织或培养组织当量浸入冷冻保护剂溶液中，搅拌冷冻保护剂溶液和浸入的组织以实现冷冻保护剂溶液有效穿透到组织中，然后以非常低的冷冻速率在0.3℃下冷冻组织 C.或更少/分钟。 在冷冻步骤中，通过播种开始细胞外冰的形成。 冷冻保存的组织可以在使用前储存无限期。 培养的组织等价物是等效人体组织的体外模型，例如皮肤或角膜，当从储存中取出时可以用于体内移植或植入或用于体外筛选化合物。

    SYSTEMS, DEVICES, AND METHODS FOR SPECIFIC CAPTURE AND RELEASE OF BIOLOGICAL SAMPLE COMPONENTS
    87.
    发明申请
    SYSTEMS, DEVICES, AND METHODS FOR SPECIFIC CAPTURE AND RELEASE OF BIOLOGICAL SAMPLE COMPONENTS 审中-公开
    用于特定捕获和释放生物样品成分的系统，设备和方法

    公开(公告)号：US20120270209A1

    公开(公告)日：2012-10-25

    申请号：US13320732

    申请日：2010-05-14

    申请人： Ajay Shah ,  Kenneth T. Kotz ,  Mehmet Toner

    发明人： Ajay Shah ,  Kenneth T. Kotz ,  Mehmet Toner

    IPC分类号： C12N5/071 ,  C12N5/09 ,  C12N5/078 ,  B05D3/10 ,  C12Q1/68 ,  C12M1/00 ,  G01N21/62 ,  G01N33/53 ,  C12N5/0783

    CPC分类号： G01N33/54393 ,  C12N11/04 ,  G01N33/54353

    摘要： Living cells can be selectively and reversibly bound to functionalized dissolvable material (e.g., cross-linked hydrogel compositions) and subsequently released from the composition as viable cells. In some examples, the cells are released by reducing the degree of cross-linking within a functionalized hydrogel composition and/or dissolving the functionalized hydrogel composition bound to the cells. The functionalized hydrogel compositions can be adhered to silicon- and silicon-oxide containing surfaces, such as glass and aminated silicon. The living cells can be isolated from biological samples, such as blood, by selectively binding certain cells from the sample to the functionalized hydrogel, removing unbound cells and later releasing viable bound cells from the functionalized hydrogel.

    摘要翻译： 活性细胞可以选择性地和可逆地结合到官能化的可溶解材料（例如，交联的水凝胶组合物）上，随后从组合物中释放为活细胞。 在一些实例中，通过降低功能化水凝胶组合物内的交联度和/或溶解与细胞结合的官能化水凝胶组合物来释放细胞。 官能化水凝胶组合物可以粘附到含硅和氧化硅的表面，例如玻璃和胺化硅。 可以通过选择性地将样品中的某些细胞与功能化的水凝胶结合，去除未结合的细胞并随后从功能化的水凝胶中释放活的结合细胞，从而将活细胞与生物样品例如血液分离。

    CAPTURING PARTICLES
    88.
    发明申请
    CAPTURING PARTICLES 有权
    捕获颗粒

    公开(公告)号：US20110294187A1

    公开(公告)日：2011-12-01

    申请号：US13121130

    申请日：2009-09-25

    申请人： Mehmet Toner

    发明人： Mehmet Toner

    IPC分类号： C12N11/02 ,  C12M3/00 ,  C12N11/00

    CPC分类号： G01N1/405 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2400/0487 ,  B01L2400/086 ,  G01N33/54366 ,  G01N33/56966 ,  G01N33/57434

    摘要： Methods and systems capturing particles suspended in a fluid flowed through a micro-channel, can include flowing the fluid including the particles to be captured through a micro-channel and past a groove defined in a surface of a wall of the micro-channel such that flowing the fluid past the groove forms microvortices in the fluid; contacting at least some of the particles against an adherent disposed on one or more of walls of the microchannel after the microvortices form in the fluid; and capturing at least some of the particles contacting the adherent.

    摘要翻译： 捕获悬浮在流过微通道的流体中的颗粒的方法和系统可以包括使包含要捕获的颗粒的流体流过微通道并经过限定在微通道壁的表面中的凹槽，使得 使流体流过凹槽，形成微流体; 在微流体在流体中形成之后，使至少一些颗粒抵靠设置在微通道的一个或多个壁上的粘附剂; 并捕获与粘附剂接触的至少一些颗粒。