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    Miniaturized Lateral Flow Device for Rapid and Sensitive Detection of Proteins or Nucleic Acids
    84.
    发明申请
    Miniaturized Lateral Flow Device for Rapid and Sensitive Detection of Proteins or Nucleic Acids 审中-公开

    公开(公告)号：US20170160271A1

    公开(公告)日：2017-06-08

    申请号：US15431705

    申请日：2017-02-13

    申请人： Los Alamos National Security, LLC.

    发明人： Robert B. Cary

    IPC分类号： G01N33/52 ,  C12Q1/68

    CPC分类号： G01N33/523 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2565/625

    摘要： The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay.

    DIRECT QUANTIFICATION OF UNPROCESSED NUCLEIC ACID SAMPLES
    86.
    发明申请
    DIRECT QUANTIFICATION OF UNPROCESSED NUCLEIC ACID SAMPLES 审中-公开
    直接测定不含有核酸的样品

    公开(公告)号：US20160251709A1

    公开(公告)日：2016-09-01

    申请号：US15029088

    申请日：2014-09-15

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Jason Yingjie LIU

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2561/113 ,  C12Q2565/607 ,  C12Q2565/625

    摘要： A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.

    摘要翻译： 本文公开了未加工的法医案件样本的直接qPCR定量的工作流程。 已经通过从纸基质的直接扩增检测到13pg的DNA。 未经处理的法医案件样本的直接qPCR定量和在相同PE拭子上收集的未加工的法医案件样本的直接STR扩增将大大增加法医实验室的效率和能力。

    Non-mass determined base compositions for nucleic acid detection
    87.
    发明授权
    Non-mass determined base compositions for nucleic acid detection 有权
    用于核酸检测的非质量确定的基础组合物

    公开(公告)号：US09080209B2

    公开(公告)日：2015-07-14

    申请号：US13389139

    申请日：2010-08-06

    申请人： Christopher C. Sappenfield

    发明人： Christopher C. Sappenfield

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2537/165 ,  C12Q2521/101 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2563/125 ,  C12Q2565/301 ,  C12Q2565/518 ,  C12Q2565/625 ,  C12Q2565/631

    摘要： The present invention provides systems, methods, and compositions for nucleic acid detection based on non-mass determined base compositions. For example, in certain embodiments, base count data for a template nucleic acid is generated using an approach that does not measure molecular mass of the template nucleic acid (e.g., by sequencing the template nucleic acid) and a database comprising base count entries is queried to identify the target nucleic acid. In particular embodiments, sequencing is employed which is conducted in substantially real-time.

    摘要翻译： 本发明提供了基于非质量确定的基础组合物的用于核酸检测的系统，方法和组合物。 例如，在某些实施方案中，使用不测量模板核酸分子量（例如通过测序模板核酸）的方法产生模板核酸的碱基计数数据，并且查询包含碱基计数条目的数据库 以鉴定靶核酸。 在具体实施方案中，采用基本上实时进行的测序。

    DIRECT QUANTIFICATION OF UNPROCESSED NUCLEIC ACID SAMPLES
    88.
    发明申请
    DIRECT QUANTIFICATION OF UNPROCESSED NUCLEIC ACID SAMPLES 审中-公开
    直接测定不含有核酸的样品

    公开(公告)号：US20150111214A1

    公开(公告)日：2015-04-23

    申请号：US14056921

    申请日：2013-10-17

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Jason Yingjie LIU

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2561/113 ,  C12Q2565/607 ,  C12Q2565/625

    摘要： A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13 pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.

    摘要翻译： 本文公开了未加工的法医案件样本的直接qPCR定量的工作流程。 已经通过从纸基质直接扩增检测到13pg的DNA。 未经处理的法医案件样本的直接qPCR定量和在相同PE拭子上收集的未加工的法医案件样本的直接STR扩增将大大增加法医实验室的效率和能力。

    DETECTION SIGNAL AND CAPTURE IN DIPSTICK ASSAYS
    89.
    发明申请
    DETECTION SIGNAL AND CAPTURE IN DIPSTICK ASSAYS 审中-公开
    检测信号和捕获在DIPSTICK测定

    公开(公告)号：US20150099265A1

    公开(公告)日：2015-04-09

    申请号：US14134422

    申请日：2013-12-19

    申请人： Helen Lee ,  Magda Anastassova Dineva

    发明人： Helen Lee ,  Magda Anastassova Dineva

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  B01L3/5023 ,  C12Q1/6816 ,  C12Q2565/625

    摘要： Improved dipstick assays for testing for the presence of a target nucleic acid in a sample solution are described. A dipstick is provided which comprises a contact end for contacting the sample solution and a capture zone remote from the contact end for capturing target nucleic acid. Sample solution is contacted with the contact end to cause sample solution to move by capillary action to the capture zone. Target nucleic acid in the sample solution is captured at the capture zone and is detected by a plurality of different labelled detection probes each capable of hybridizing to a different region of the target nucleic acid. The detection signal is thereby enhanced. In other methods a plurality of different capture probes are added to the sample solution which can then be bound by a capture moiety at the capture zone to indirectly capture target nucleic acid. Capture of target nucleic acid is thereby improved. Kits and dipsticks for carrying out such methods are also described.

    摘要翻译： 描述了用于测试样品溶液中靶核酸的存在的改进的测试仪测定。 提供了一种量油尺，其包括用于接触样品溶液的接触端和远离接触端的捕获区域，用于捕获靶核酸。 样品溶液与接触端接触，使样品溶液通过毛细作用移动到捕获区。 在捕获区捕获样品溶液中的靶核酸，并且通过多个不同的标记的检测探针检测，每个能够与靶核酸的不同区域杂交。 从而增强检测信号。 在其它方法中，将多个不同的捕获探针加入到样品溶液中，然后可以在捕获区被俘获部分结合以间接捕获靶核酸。 从而改善目标核酸的捕获。 还描述了用于实施这些方法的套件和骰子。

    DETECTION OF NUCLEIC ACID AMPLIFICATION IN A POROUS SUBSTRATE
    90.
    发明申请
    DETECTION OF NUCLEIC ACID AMPLIFICATION IN A POROUS SUBSTRATE 有权
    检测多孔基质中的核酸扩增

    公开(公告)号：US20150050654A1

    公开(公告)日：2015-02-19

    申请号：US13970315

    申请日：2013-08-19

    申请人： General Electric Company

    发明人： Erin Jean Finehout ,  Christopher Michael Puleo ,  Ralf Lenigk ,  Jason M. Nichols ,  John Nelson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q2565/137 ,  C12Q2565/625 ,  C12Q2527/113 ,  C12Q2565/133 ,  C12Q2545/114

    摘要： The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated.

    摘要翻译： 本公开涉及通过多孔底物中的扩增检测来表征生物样品。 举例来说，多孔底物可以包括配置成在放大期间释放时提供信号的扩增试剂。 当应用样品时，从应用点开始发生波前放大，波前在多孔基底上达到距离的时间与施加样品的初始浓度有关。 通过在一个或多个时间点检测扩增产物行进的距离，可以估计样品的初始浓度。