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公开(公告)号：US20100263732A1
公开(公告)日：2010-10-21
申请号：US12762170
申请日：2010-04-16
IPC分类号： F17D1/00
CPC分类号： C30B7/14 , B01F5/00 , B01F13/0093 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502738 , B01L3/502746 , B01L3/502769 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B81B1/00 , C30B29/02 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0086 , Y10T117/1004 , Y10T117/1008 , Y10T117/1012 , Y10T137/0318 , Y10T137/0324 , Y10T137/2224 , Y10T436/25
摘要： A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.
摘要翻译： 将静态流体和第二流体沿微流体界面放置接触，并允许通过扩散混合而不对流流过该界面。 根据本发明的一个实施例，流体是静态的，并且最初位于微流体通道中的封闭阀结构的任一侧上，该微流体通道的宽度被紧紧地约束在至少一个维度上。 然后打开阀门，并且微流体通道两侧的防滑层抑制沿着所得界面的两种流体之间的对流混合。 根据本发明的实施方案的用于微流体自由界面的应用包括但不限于蛋白质结晶研究，蛋白质溶解度研究，流体系统的性质的确定以及各种生物测定如扩散免疫测定，底物转换测定 和竞争性结合测定。
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公开(公告)号：US07479186B2
公开(公告)日：2009-01-20
申请号：US11415672
申请日：2006-05-01
IPC分类号： C30B29/54
CPC分类号： C30B29/00 , B01D9/00 , B01D9/0072 , B01D9/0077 , B01J19/0046 , B01J2219/00274 , B01J2219/00286 , B01J2219/00317 , B01J2219/00389 , B01J2219/00418 , B01J2219/00432 , B01J2219/00585 , B01J2219/00756 , B01J2219/00891 , B01J2219/00907 , B01J2219/00952 , B01L3/06 , B01L3/5025 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B81B2201/051 , B81B2201/054 , B81B2203/0127 , B81B2203/0315 , B81C1/00119 , B81C99/0065 , C30B7/14 , C30B29/54 , C30B29/58 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , Y10T117/10 , Y10T117/1004 , Y10T117/1008 , Y10T117/1024
摘要： High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.
摘要翻译： 目标材料的结晶化的高通量筛选是通过将目标材料的溶液同时引入微细加工的流体装置的多个室来实现的。 然后对微制造的流体装置进行操作以改变室中的溶液状态，从而同时提供大量的结晶环境。 改变的溶液条件的控制可以由各种技术产生，包括但不限于通过体积排阻将结晶剂计量到室中的体积，通过捕获通过微结构结构的尺寸确定的结晶剂的体积， 将样品和结晶剂通道注入由相交的正交流动通道限定的连接阵列。
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公开(公告)号：US07195670B2
公开(公告)日：2007-03-27
申请号：US10117978
申请日：2002-04-05
IPC分类号： C30B7/14
CPC分类号： C30B7/08 , B01J2219/00274 , B01L3/06 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L3/502761 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/0642 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2300/1827 , B01L2400/0481 , B01L2400/049 , B01L2400/0638 , B01L2400/0655 , B01L2400/0688 , C12Q1/6874 , C30B7/14 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0026 , F16K99/0034 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T117/1004 , Y10T117/1008 , Y10T137/0318 , Y10T137/0396 , C12Q2535/125
摘要： High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.
摘要翻译： 目标材料的结晶化的高通量筛选是通过将目标材料的溶液同时引入微细加工的流体装置的多个室来实现的。 然后对微制造的流体装置进行操作以改变室中的溶液状态，从而同时提供大量的结晶环境。 改变的溶液条件的控制可以由各种技术产生，包括但不限于通过体积排阻将结晶剂计量到室中的体积，通过捕获通过微结构结构的尺寸确定的结晶剂的体积， 将样品和结晶剂通道注入由相交的正交流动通道限定的连接阵列。
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公开(公告)号：US20120241015A1
公开(公告)日：2012-09-27
申请号：US13280276
申请日：2011-10-24
IPC分类号： F17D1/00
CPC分类号： C30B7/14 , F17D5/005 , F17D5/06 , Y10T137/0318 , Y10T137/0391
摘要： The present invention relates to microfluidic devices and methods facilitating the growth and analysis of crystallized materials such as proteins. In accordance with one embodiment, a crystal growth architecture is separated by a permeable membrane from an adjacent well having a much larger volume. The well may be configured to contain a fluid having an identity and concentration similar to the solvent and crystallizing agent employed in crystal growth, with diffusion across the membrane stabilizing that process. Alternatively, the well may be configured to contain a fluid having an identity calculated to affect the crystallization process. In accordance with the still other embodiment, the well may be configured to contain a material such as a cryo-protectant, which is useful in protecting the crystalline material once formed.
摘要翻译： 本发明涉及促进结晶物质如蛋白质生长和分析的微流体装置和方法。 根据一个实施例，晶体生长结构由具有大得多体积的相邻阱的可渗透膜分离。 该孔可以被配置成包含具有与晶体生长中使用的溶剂和结晶剂相似的特性和浓度的流体，并且通过膜的扩散稳定了该过程。 或者，孔可以被配置为包含具有计算以影响结晶过程的同一性的流体。 根据另一个实施方案，所述孔可以被配置为容纳诸如冷冻保护剂的材料，其可用于在形成后保护结晶材料。
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公开(公告)号：US20120046639A1
公开(公告)日：2012-02-23
申请号：US13213990
申请日：2011-08-19
IPC分类号： A61M37/00 , G01N33/53 , G01N33/68 , F17D3/00 , C12M1/34 , C07K1/34 , G01N33/50 , G01N33/00 , C12Q1/02 , C12Q1/34
CPC分类号： C30B7/14 , B01F5/00 , B01F13/0093 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502738 , B01L3/502746 , B01L3/502769 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B81B1/00 , C30B29/02 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0086 , Y10T117/1004 , Y10T117/1008 , Y10T117/1012 , Y10T137/0318 , Y10T137/0324 , Y10T137/2224 , Y10T436/25
摘要： A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.
摘要翻译： 将静态流体和第二流体沿微流体界面放置接触，并允许通过扩散混合而不对流流过该界面。 根据本发明的一个实施例，流体是静态的，并且最初位于微流体通道中的封闭阀结构的任一侧上，该微流体通道的宽度被紧紧地约束在至少一个维度上。 然后打开阀门，并且微流体通道两侧的防滑层抑制沿着所得界面的两种流体之间的对流混合。 根据本发明的实施方案的用于微流体自由界面的应用包括但不限于蛋白质结晶研究，蛋白质溶解度研究，流体系统的性质的确定以及各种生物测定，例如扩散免疫测定，底物转换测定 和竞争性结合测定。
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公开(公告)号：US20080182273A1
公开(公告)日：2008-07-31
申请号：US12001768
申请日：2007-12-11
IPC分类号： G01N33/53 , G01N33/536
CPC分类号： C30B7/14 , B01F5/00 , B01F13/0093 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502738 , B01L3/502746 , B01L3/502769 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B81B1/00 , C30B29/02 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0086 , Y10T117/1004 , Y10T117/1008 , Y10T117/1012 , Y10T137/0318 , Y10T137/0324 , Y10T137/2224 , Y10T436/25
摘要： A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.
摘要翻译： 将静态流体和第二流体沿微流体界面放置接触，并允许通过扩散混合而不对流流过该界面。 根据本发明的一个实施例，流体是静态的，并且最初位于微流体通道中的封闭阀结构的任一侧上，该微流体通道的宽度被紧紧地约束在至少一个维度上。 然后打开阀门，并且微流体通道两侧的防滑层抑制沿着所得界面的两种流体之间的对流混合。 根据本发明的实施方案的用于微流体自由界面的应用包括但不限于蛋白质结晶研究，蛋白质溶解度研究，流体系统的性质的确定以及各种生物测定如扩散免疫测定，底物转换测定 和竞争性结合测定。
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公开(公告)号：US07306672B2
公开(公告)日：2007-12-11
申请号：US10265473
申请日：2002-10-04
IPC分类号： C30B7/14
CPC分类号： C30B7/14 , B01F5/00 , B01F13/0093 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502738 , B01L3/502746 , B01L3/502769 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B81B1/00 , C30B29/02 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0086 , Y10T117/1004 , Y10T117/1008 , Y10T117/1012 , Y10T137/0318 , Y10T137/0324 , Y10T137/2224 , Y10T436/25
摘要： A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.
摘要翻译： 将静态流体和第二流体沿微流体界面放置接触，并允许通过扩散混合而不对流流过该界面。 根据本发明的一个实施例，流体是静态的，并且最初位于微流体通道中的封闭阀结构的任一侧上，该微流体通道的宽度被紧紧地约束在至少一个维度上。 然后打开阀门，并且微流体通道两侧的防滑层抑制沿着所得界面的两种流体之间的对流混合。 根据本发明的实施方案的用于微流体自由界面的应用包括但不限于蛋白质结晶研究，蛋白质溶解度研究，流体系统的性质的确定以及各种生物测定如扩散免疫测定，底物转换测定 和竞争性结合测定。
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公开(公告)号：US08709152B2
公开(公告)日：2014-04-29
申请号：US13213990
申请日：2011-08-19
IPC分类号： C30B7/14
CPC分类号： C30B7/14 , B01F5/00 , B01F13/0093 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502738 , B01L3/502746 , B01L3/502769 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B81B1/00 , C30B29/02 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0086 , Y10T117/1004 , Y10T117/1008 , Y10T117/1012 , Y10T137/0318 , Y10T137/0324 , Y10T137/2224 , Y10T436/25
摘要： A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.
摘要翻译： 将静态流体和第二流体沿微流体界面放置接触，并允许通过扩散混合而不对流流过该界面。 根据本发明的一个实施例，流体是静态的，并且最初位于微流体通道中的封闭阀结构的任一侧上，该微流体通道的宽度被紧紧地约束在至少一个维度上。 然后打开阀门，并且微流体通道两侧的防滑层抑制沿着所得界面的两种流体之间的对流混合。 根据本发明的实施方案的用于微流体自由界面的应用包括但不限于蛋白质结晶研究，蛋白质溶解度研究，流体系统的性质的确定以及各种生物测定如扩散免疫测定，底物转换测定 和竞争性结合测定。
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公开(公告)号：US08382896B2
公开(公告)日：2013-02-26
申请号：US11668263
申请日：2007-01-29
IPC分类号： C30B7/08
CPC分类号： C30B7/08 , B01J2219/00274 , B01L3/06 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L3/502761 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/0642 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2300/1827 , B01L2400/0481 , B01L2400/049 , B01L2400/0638 , B01L2400/0655 , B01L2400/0688 , C12Q1/6874 , C30B7/14 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0026 , F16K99/0034 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T117/1004 , Y10T117/1008 , Y10T137/0318 , Y10T137/0396 , C12Q2535/125
摘要： High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.
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公开(公告)号：US07670429B2
公开(公告)日：2010-03-02
申请号：US11103599
申请日：2005-04-12
IPC分类号： C30B29/54
CPC分类号： C30B29/54 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00756 , B01L3/06 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688
摘要： High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.
摘要翻译： 目标材料的结晶化的高通量筛选是通过将目标材料的溶液同时引入微细加工的流体装置的多个室来实现的。 然后对微制造的流体装置进行操作以改变室中的溶液状态，从而同时提供大量的结晶环境。 改变的溶液条件的控制可以由各种技术产生，包括但不限于通过体积排阻将结晶剂计量到室中的体积，通过捕获通过微结构结构的尺寸确定的结晶剂的体积， 将样品和结晶剂通道注入由相交的正交流动通道限定的连接阵列。
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