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1.发明授权Methods and systems for processing microfluidic samples of particle containing fluids 有权用于加工含微粒流体微流体样品的方法和系统公开(公告)号：US07270786B2
公开(公告)日：2007-09-18
申请号：US10014520
申请日：2001-12-14
申请人： Gene Parunak , Kalyan Handique , Betty Wu , Aaron Kehrer
发明人： Gene Parunak , Kalyan Handique , Betty Wu , Aaron Kehrer
CPC分类号： F04B19/006 , B01L3/502738 , B01L3/502784 , B01L7/52 , B01L2200/0647 , B01L2200/10 , B01L2300/0681 , B01L2300/1805 , B01L2400/0442 , B01L2400/0487 , B01L2400/0633 , B01L2400/0688 , F04B19/24 , F15C3/002 , F16K99/0001 , F16K99/0017 , F16K99/0036 , F16K99/0044 , F16K2099/0074 , F16K2099/0076 , G01N1/4077 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , Y10T436/25 , Y10T436/25375
摘要： The present invention relates to a microfluidic system for processing a cell-containing liquid. The system includes a microfabricated substrate having an enrichment channel to prepare an enriched cell sample from the cell-containing liquid. A flow through member is in liquid communication with the enrichment zone. The flow through member substantially prevents cells of the cell-containing fluid from exiting the enrichment zone while allowing liquid of the cell-containing liquid to exit the enrichment zone. A gas actuator associated with the enrichment zone provides a gas pressure sufficient to move the enriched cell sample from the enrichment zone.
摘要翻译： 本发明涉及用于处理含细胞液体的微流体系统。 该系统包括具有富集通道的微制造底物，以从含细胞的液体制备富集的细胞样品。 流通构件与富集区液体连通。 流通构件基本上防止含细胞液体的细胞离开富集区，同时允许含细胞液体的液体离开富集区。 与富集区相关联的气体致动器提供足以将富集的细胞样品从富集区移动的气体压力。
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公开(公告)号：US20060166233A1
公开(公告)日：2006-07-27
申请号：US11281247
申请日：2005-11-16
申请人： Betty Wu , John Althaus , Nikhil Phadke , Sundaresh Brahmasandra , Kalyan Handique , Aaron Kehrer , Gene Parunak , Cecelia Haley , Ted Springer
发明人： Betty Wu , John Althaus , Nikhil Phadke , Sundaresh Brahmasandra , Kalyan Handique , Aaron Kehrer , Gene Parunak , Cecelia Haley , Ted Springer
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L3/523 , B01L7/52 , B01L2200/16 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0683 , C12N15/101 , F16K99/0001 , F16K99/0019 , F16K99/0034 , F16K99/0044 , F16K2099/0084
摘要： Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
摘要翻译： 公开了用于处理多核苷酸（例如DNA）的方法和系统。 处理区域包括用在第一组条件（例如温度和pH）下保留多核苷酸的配体修饰的一个或多个表面（例如，颗粒表面），并在第二组条件（例如较高温度和/ 或更多的碱性pH）。 处理区域可以用于例如浓缩样品的多核苷酸和/或来自多核苷酸的单独的扩增反应抑制剂。 公开了具有处理区域的微流体装置。
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公开(公告)号：US08679831B2
公开(公告)日：2014-03-25
申请号：US12702648
申请日：2010-02-09
申请人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
发明人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502738 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0677 , B33Y40/00 , B33Y80/00 , C12Q2523/109 , G01N1/40 , Y10T436/25 , Y10T436/25375
摘要： A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
摘要翻译： 微流体装置包括用于将含颗粒的液体样品输入到装置中的输入端口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含有颗粒的液体样品的颗粒与含有流体样品的颗粒的液体的第一部分空间分离。 压力执行器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的一部分重新组合。 该装置还可以包括从保留构件接收颗粒和液体的裂解室。 裂解室热裂解颗粒以释放其内容物。
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公开(公告)号：US08852862B2
公开(公告)日：2014-10-07
申请号：US11281247
申请日：2005-11-16
申请人： Betty Wu , John S. Althaus , Nikhil Phadke , Sundaresh N. Brahmasandra , Kalyan Handique , Aaron Kehrer , Gene Parunak , Cecelia Haley , Ted Springer
发明人： Betty Wu , John S. Althaus , Nikhil Phadke , Sundaresh N. Brahmasandra , Kalyan Handique , Aaron Kehrer , Gene Parunak , Cecelia Haley , Ted Springer
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L3/523 , B01L7/52 , B01L2200/16 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0683 , C12N15/101 , F16K99/0001 , F16K99/0019 , F16K99/0034 , F16K99/0044 , F16K2099/0084
摘要： Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
摘要翻译： 公开了用于处理多核苷酸（例如DNA）的方法和系统。 处理区域包括用在第一组条件（例如温度和pH）下保留多核苷酸的配体修饰的一个或多个表面（例如，颗粒表面），并在第二组条件（例如较高温度和/ 或更多的碱性pH）。 处理区域可以用于例如浓缩样品的多核苷酸和/或来自多核苷酸的单独的扩增反应抑制剂。 公开了具有处理区域的微流体装置。
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公开(公告)号：US07731906B2
公开(公告)日：2010-06-08
申请号：US10567002
申请日：2004-08-02
申请人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
发明人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
IPC分类号： B01L3/02
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502738 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0677 , B33Y40/00 , B33Y80/00 , C12Q2523/109 , G01N1/40 , Y10T436/25 , Y10T436/25375
摘要： A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
摘要翻译： 微流体装置包括用于将含颗粒的液体样品输入到装置中的输入端口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含有颗粒的液体样品的颗粒与含有流体样品的颗粒的液体的第一部分空间分离。 压力执行器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的一部分重新组合。 该装置还可以包括从保留构件接收颗粒和液体的裂解室。 裂解室热裂解颗粒以释放其内容物。
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公开(公告)号：US20060205085A1
公开(公告)日：2006-09-14
申请号：US10567002
申请日：2004-08-02
申请人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
发明人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502738 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0677 , B33Y40/00 , B33Y80/00 , C12Q2523/109 , G01N1/40 , Y10T436/25 , Y10T436/25375
摘要： A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
摘要翻译： 微流体装置包括用于将含颗粒的液体样品输入到装置中的输入端口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含有颗粒的液体样品的颗粒与含有流体样品的颗粒的液体的第一部分空间分离。 压力执行器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的一部分重新组合。 该装置还可以包括从保留构件接收颗粒和液体的裂解室。 裂解室热裂解颗粒以释放其内容物。
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公开(公告)号：US20100197008A1
公开(公告)日：2010-08-05
申请号：US12702648
申请日：2010-02-09
申请人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
发明人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502738 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0677 , B33Y40/00 , B33Y80/00 , C12Q2523/109 , G01N1/40 , Y10T436/25 , Y10T436/25375
摘要： A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
摘要翻译： 微流体装置包括用于将含颗粒的液体样品输入到装置中的输入端口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含有颗粒的液体样品的颗粒与含有流体样品的颗粒的液体的第一部分空间分离。 压力执行器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的一部分重新组合。 该装置还可以包括从保留构件接收颗粒和液体的裂解室。 裂解室热裂解颗粒以释放其内容物。
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8.发明授权Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids 有权用于从流体微流体样品中的细胞释放细胞内物质的方法和系统公开(公告)号：US07192557B2
公开(公告)日：2007-03-20
申请号：US10014519
申请日：2001-12-14
申请人： Betty Wu , Karthik Ganesan , Kalyan Handique , Gene Parunak
发明人： Betty Wu , Karthik Ganesan , Kalyan Handique , Gene Parunak
CPC分类号： G01N1/4077 , B01L3/50273 , B01L3/502738 , B01L3/502784 , B01L7/52 , B01L2200/0647 , B01L2200/10 , B01L2300/0681 , B01L2300/1805 , B01L2400/0442 , B01L2400/0487 , B01L2400/0633 , B01L2400/0688 , F04B19/006 , F04B19/24 , Y10T436/11 , Y10T436/25 , Y10T436/25375
摘要： The present invention relates to a microfluidic system for processing a cell-containing liquid. The system includes a lysing zone to receive the cell-containing sample and a positioning element to position the cell-containing sample in a lysing position in the vicinity of a lysing mechanism. The lysing mechanism releases intracellular material, such as DNA or RNA, from the cells. In one embodiment, the lysing mechanism includes electrodes for generating an electric field sufficient to release intracellular contents from the cells. Alternatively, the lysing mechanism may lyse the cells using chemical, heat and/or ultrasonic techniques or any combination of these techniques.
摘要翻译： 本发明涉及用于处理含细胞液体的微流体系统。 该系统包括用于接收含细胞的样品的裂解区和定位元件，以将含细胞的样品定位在裂解机制附近的裂解位置。 裂解机制从细胞中释放细胞内的物质，如DNA或RNA。 在一个实施方案中，裂解机制包括用于产生足以从细胞释放细胞内内容物的电场的电极。 或者，裂解机制可以使用化学，热和/或超声技术或这些技术的任何组合来裂解细胞。
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9.发明授权Integrated system for processing microfluidic samples, and method of using the same 有权用于加工微流体样品的集成系统及其使用方法公开(公告)号：US09040288B2
公开(公告)日：2015-05-26
申请号：US11728964
申请日：2007-03-26
申请人： Kalyan Handique , Sundaresh N. Brahmasandra , Karthik Ganesan , Betty Wu , Nikhil Phadke , Gene Parunak , Jeff Williams
发明人： Kalyan Handique , Sundaresh N. Brahmasandra , Karthik Ganesan , Betty Wu , Nikhil Phadke , Gene Parunak , Jeff Williams
CPC分类号： B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/10 , B01L2200/147 , B01L2200/148 , B01L2200/16 , B01L2300/021 , B01L2300/06 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2300/18 , B01L2300/1827 , B01L2300/1861 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0611 , B01L2400/0677 , B01L2400/0683 , F16K99/0001 , F16K99/003 , F16K99/0032 , F16K99/0044 , F16K99/0061 , F16K2099/0084 , G01N2035/00881
摘要： This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
摘要翻译： 该专利申请描述了用于处理含有多核苷酸的样品并用于在其上提供诊断结果的集成设备。 该装置被配置为接收包含用于处理样品的试剂和网络的微流控盒。 还描述了使用该装置的方法。
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10.发明申请Integrated system for processing microfluidic samples, and method of using the same 有权用于加工微流体样品的集成系统及其使用方法公开(公告)号：US20070292941A1
公开(公告)日：2007-12-20
申请号：US11728964
申请日：2007-03-26
申请人： Kalyan Handique , Sundaresh Brahmasandra , Karthik Ganesan , Betty Wu , Nikhil Phadke , Gene Parunak , Jeff Williams
发明人： Kalyan Handique , Sundaresh Brahmasandra , Karthik Ganesan , Betty Wu , Nikhil Phadke , Gene Parunak , Jeff Williams
IPC分类号： C12M1/34
CPC分类号： B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/10 , B01L2200/147 , B01L2200/148 , B01L2200/16 , B01L2300/021 , B01L2300/06 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2300/18 , B01L2300/1827 , B01L2300/1861 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0611 , B01L2400/0677 , B01L2400/0683 , F16K99/0001 , F16K99/003 , F16K99/0032 , F16K99/0044 , F16K99/0061 , F16K2099/0084 , G01N2035/00881
摘要： This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
摘要翻译： 该专利申请描述了用于处理含有多核苷酸的样品并用于在其上提供诊断结果的集成设备。 该装置被配置为接收包含用于处理样品的试剂和网络的微流控盒。 还描述了使用该装置的方法。
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