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    Cyclone aerosol sampler and biological aerosol chemiluminescent detection system employing the same
    4.
    发明授权
    Cyclone aerosol sampler and biological aerosol chemiluminescent detection system employing the same 失效
    旋风式气溶胶采样器和采用该气溶胶的生物气溶胶化学发光检测系统

    公开(公告)号：US6103534A

    公开(公告)日：2000-08-15

    申请号：US407372

    申请日：1999-09-28

    申请人： David A. Stenger ,  James P. Whelan

    发明人： David A. Stenger ,  James P. Whelan

    IPC分类号： G01N21/76

    CPC分类号： G01N21/76 ,  Y10T436/25875

    摘要： An improved biological aerosol chemiluminescent (CL) detection system having optimal sensitivity and a method for using the same in detecting the presence of biological materials in an aerosol sample. The detection system comprises a cyclone aerosol sampler, a CL reagent injector, and a luminometer, wherein the CL reagent injector is mated with the cyclone aerosol sampler so as to deliver/introduce CL reagent thereto. The inner surface of the cyclone aerosol sampler and the inner surface of the CL reagent injector are fabricated from materials that are free of metals that would initiate a CL-based reaction with a CL reagent to be employed therein so as to provide a CL reagent path free of these metals. The entire CL reagent path in the biological aerosol CL detection apparatus is comprised of these materials. A method for determining the suitability of materials for use throughout the CL reagent path of the biological CL detection system is also taught.

    摘要翻译： 具有最佳灵敏度的改进的生物气溶胶化学发光（CL）检测系统及其用于检测气溶胶样品中生物材料的存在的方法。 检测系统包括旋风式气溶胶取样器，CL试剂注射器和发光计，其中CL试剂注射器与旋风分离器气溶胶取样器配合，以便向其递送/引入CL试剂。 旋流气溶胶取样器的内表面和CL试剂注射器的内表面由不含金属的材料制成，这些材料将引发与其中使用的CL试剂的基于CL的反应，以提供CL试剂路径 没有这些金属。 生物气溶胶CL检测装置中的整个CL试剂路径由这些材料组成。 还教导了一种用于确定在生物CL检测系统的CL试剂路径上使用的材料的适用性的方法。

    METHOD FOR DIRECT CAPTURE OF RIBONUCLEIC ACID
    6.
    发明申请
    METHOD FOR DIRECT CAPTURE OF RIBONUCLEIC ACID 审中-公开
    直接捕获RIBONUCLEIC ACID的方法

    公开(公告)号：US20100112643A1

    公开(公告)日：2010-05-06

    申请号：US12264462

    申请日：2008-11-04

    申请人： Marie J. Archer ,  Baochuan Lin ,  David A. Stenger

    发明人： Marie J. Archer ,  Baochuan Lin ,  David A. Stenger

    IPC分类号： C12P19/34 ,  C07H21/02

    CPC分类号： C40B50/18 ,  C12Q1/6813

    摘要： A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a target ribonucleic acid; denaturing the target ribonucleic acid; and incubating the sample to allow hybridization of the denatured ribonucleic acid to the probe nucleic acids. The target ribonucleic acid is complementary to the probe nucleic acid.

    摘要翻译： 一种方法：提供具有结合到其上的树枝状大分子分子的固体表面和固定在树状聚合物上的单链探针核酸; 使固体表面与疑似或已知含有目标核糖核酸的样品接触; 使目标核糖核酸变性; 并培养样品以允许变性核糖核酸与探针核酸杂交。 靶核糖核酸与探针核酸互补。

    Immobilization of oligonucleotides and proteins in sugar-containing hydrogels
    8.
    发明授权
    Immobilization of oligonucleotides and proteins in sugar-containing hydrogels 有权
    寡糖和蛋白质在含糖水凝胶中的固定

    公开(公告)号：US07785769B2

    公开(公告)日：2010-08-31

    申请号：US10627143

    申请日：2003-07-25

    申请人： Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

    发明人： Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

    IPC分类号： C12Q1/00

    CPC分类号： G01N33/548 ,  G01N33/54353

    摘要： The use of sugar-containing hydrogels as very highly porous, aqueous support material for the immobilization of oligonucleotides, peptides, proteins, antigens, antibodies, polysaccharides, and other biomolecules for sensor applications. Unusually large sizes of interconnected pores allow large target molecules to pass rapidly into and through the gel and bind to immobilized biomolecules. Sugar-containing hydrogels have extremely low non-specific absorption of labeled target molecules, providing low background levels. Some hydrogel materials do not have this type of homogeneous interconnected macroporosity, thus large target molecules cannot readily diffuse through them. Additionally, they nearly always experience non-specific absorption of labeled target molecules, limiting their usefulness in sensor applications. A method is provided for preparing sugar polyacrylate hydrogels with functional chemical groups which covalently bond oligonucleotides and peptides. A method for copolymerizing acrylate-terminated oligonucleotides with sugar acrylate monomers and diacrylate cross-linking agents is also provided.

    摘要翻译： 使用含糖水凝胶作为用于固定寡核苷酸，肽，蛋白质，抗原，抗体，多糖和其他用于传感器应用的生物分子的非常高度多孔的水性载体材料。 异常大尺寸的相互连通的孔允许大的目标分子快速通过并通过凝胶并结合固定化的生物分子。 含糖水凝胶对标记的靶分子具有极低的非特异性吸收，提供低的背景水平。 一些水凝胶材料不具有这种类型的均匀相互连接的大孔隙度，因此大的靶分子不能容易地通过它们扩散。 此外，它们几乎总是经历对标记的靶分子的非特异性吸收，限制了它们在传感器应用中的用途。 提供了一种制备具有共价键合寡核苷酸和肽的功能化学基团的糖聚丙烯酸酯水凝胶的方法。 还提供了将丙烯酸酯封端的寡核苷酸与糖丙酯单体和二丙烯酸酯交联剂共聚的方法。

    SOLID PHASE FOR CAPTURE OF NUCLEIC ACIDS
    9.
    发明申请
    SOLID PHASE FOR CAPTURE OF NUCLEIC ACIDS 有权
    固体捕获核酸的相

    公开(公告)号：US20080293594A1

    公开(公告)日：2008-11-27

    申请号：US11751096

    申请日：2007-05-21

    申请人： Marie J. Archer ,  Baochuan Lin ,  David A. Stenger

    发明人： Marie J. Archer ,  Baochuan Lin ,  David A. Stenger

    IPC分类号： C40B40/08 ,  C40B50/18

    CPC分类号： C12N15/1006 ,  C07B2200/11 ,  C12N15/1013 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/08 ,  C40B50/18

    摘要： A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a double-stranded complimentary target nucleic acid; denaturing the target nucleic acids at thermal conditions and in a salt concentration sufficient to denature the target nucleic acids to produce denatured nucleic acids; and cooling the sample to allow hybridization of the denatured nucleic acids to the probe nucleic acids. An article having: one or more paramagnetic microbeads; a dendrimer molecule bound to the beads; and a probe nucleic acid immobilized to the dendrimer.

    摘要翻译： 一种方法：提供具有结合到其上的树枝状大分子分子的固体表面和固定在树状聚合物上的单链探针核酸; 使所述固体表面与疑似或已知含有双链互补靶核酸的样品接触; 在热条件下和在足以使目标核酸变性以产生变性核酸的盐浓度下使目标核酸变性; 并冷却样品以允许变性核酸与探针核酸杂交。 一种具有：一个或多个顺磁性微珠的物品; 与珠结合的树枝状大分子; 和固定在树状聚合物上的探针核酸。