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公开(公告)号：US08273308B2
公开(公告)日：2012-09-25
申请号：US11929971
申请日：2007-10-30
申请人： Kalyan Handique , Gene Parunak
发明人： Kalyan Handique , Gene Parunak
CPC分类号： C12Q1/686 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502784 , B01L7/52 , B01L2200/0605 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/10 , B01L2200/143 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/1805 , B01L2300/1827 , B01L2400/0406 , B01L2400/0442 , B01L2400/0487 , B01L2400/0633 , B01L2400/0688 , B01L2400/0694 , B01L2400/084 , F04B19/006 , F04B19/24 , G01N1/4077 , G01N2035/1044 , Y10T436/11 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： This disclosure provides systems, methods, and devices for processing samples on a microfluidic device. One system includes a microfluidic device having an upstream channel, a DNA manipulation zone located downstream from the upstream channel and configured to perform PCR amplification of a sample, a first valve disposed upstream of the DNA manipulation zone, and a second valve disposed downstream of the DNA manipulation zone. The system also includes a controller programmed to close the first and second valves to prevent gas and liquid from flowing into or out of the DNA manipulation zone, and a computer-controlled heat source in thermal contact with the DNA manipulation zone.
摘要翻译： 本公开提供了用于在微流体装置上处理样品的系统，方法和装置。 一个系统包括具有上游通道的微流体装置，位于上游通道下游的DNA操纵区，并且被配置为执行样品的PCR扩增，设置在DNA操作区上游的第一阀，以及设置在DNA操纵区下游的第二阀 DNA操纵区。 该系统还包括被编程为关闭第一和第二阀以防止气体和液体流入或移出DNA操作区的控制器，以及与DNA操作区热接触的计算机控制的热源。
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公开(公告)号：USD565742S1
公开(公告)日：2008-04-01
申请号：US29263444
申请日：2006-07-21
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3.发明授权Methods and systems for processing microfluidic samples of particle containing fluids 有权用于加工含微粒流体微流体样品的方法和系统公开(公告)号：US07270786B2
公开(公告)日：2007-09-18
申请号：US10014520
申请日：2001-12-14
申请人： Gene Parunak , Kalyan Handique , Betty Wu , Aaron Kehrer
发明人： Gene Parunak , Kalyan Handique , Betty Wu , Aaron Kehrer
CPC分类号： F04B19/006 , B01L3/502738 , B01L3/502784 , B01L7/52 , B01L2200/0647 , B01L2200/10 , B01L2300/0681 , B01L2300/1805 , B01L2400/0442 , B01L2400/0487 , B01L2400/0633 , B01L2400/0688 , F04B19/24 , F15C3/002 , F16K99/0001 , F16K99/0017 , F16K99/0036 , F16K99/0044 , F16K2099/0074 , F16K2099/0076 , G01N1/4077 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , Y10T436/25 , Y10T436/25375
摘要： The present invention relates to a microfluidic system for processing a cell-containing liquid. The system includes a microfabricated substrate having an enrichment channel to prepare an enriched cell sample from the cell-containing liquid. A flow through member is in liquid communication with the enrichment zone. The flow through member substantially prevents cells of the cell-containing fluid from exiting the enrichment zone while allowing liquid of the cell-containing liquid to exit the enrichment zone. A gas actuator associated with the enrichment zone provides a gas pressure sufficient to move the enriched cell sample from the enrichment zone.
摘要翻译： 本发明涉及用于处理含细胞液体的微流体系统。 该系统包括具有富集通道的微制造底物，以从含细胞的液体制备富集的细胞样品。 流通构件与富集区液体连通。 流通构件基本上防止含细胞液体的细胞离开富集区，同时允许含细胞液体的液体离开富集区。 与富集区相关联的气体致动器提供足以将富集的细胞样品从富集区移动的气体压力。
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公开(公告)号：US20060166233A1
公开(公告)日：2006-07-27
申请号：US11281247
申请日：2005-11-16
申请人： Betty Wu , John Althaus , Nikhil Phadke , Sundaresh Brahmasandra , Kalyan Handique , Aaron Kehrer , Gene Parunak , Cecelia Haley , Ted Springer
发明人： Betty Wu , John Althaus , Nikhil Phadke , Sundaresh Brahmasandra , Kalyan Handique , Aaron Kehrer , Gene Parunak , Cecelia Haley , Ted Springer
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L3/523 , B01L7/52 , B01L2200/16 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0683 , C12N15/101 , F16K99/0001 , F16K99/0019 , F16K99/0034 , F16K99/0044 , F16K2099/0084
摘要： Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
摘要翻译： 公开了用于处理多核苷酸（例如DNA）的方法和系统。 处理区域包括用在第一组条件（例如温度和pH）下保留多核苷酸的配体修饰的一个或多个表面（例如，颗粒表面），并在第二组条件（例如较高温度和/ 或更多的碱性pH）。 处理区域可以用于例如浓缩样品的多核苷酸和/或来自多核苷酸的单独的扩增反应抑制剂。 公开了具有处理区域的微流体装置。
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公开(公告)号：US08852862B2
公开(公告)日：2014-10-07
申请号：US11281247
申请日：2005-11-16
申请人： Betty Wu , John S. Althaus , Nikhil Phadke , Sundaresh N. Brahmasandra , Kalyan Handique , Aaron Kehrer , Gene Parunak , Cecelia Haley , Ted Springer
发明人： Betty Wu , John S. Althaus , Nikhil Phadke , Sundaresh N. Brahmasandra , Kalyan Handique , Aaron Kehrer , Gene Parunak , Cecelia Haley , Ted Springer
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L3/523 , B01L7/52 , B01L2200/16 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0683 , C12N15/101 , F16K99/0001 , F16K99/0019 , F16K99/0034 , F16K99/0044 , F16K2099/0084
摘要： Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
摘要翻译： 公开了用于处理多核苷酸（例如DNA）的方法和系统。 处理区域包括用在第一组条件（例如温度和pH）下保留多核苷酸的配体修饰的一个或多个表面（例如，颗粒表面），并在第二组条件（例如较高温度和/ 或更多的碱性pH）。 处理区域可以用于例如浓缩样品的多核苷酸和/或来自多核苷酸的单独的扩增反应抑制剂。 公开了具有处理区域的微流体装置。
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公开(公告)号：US07731906B2
公开(公告)日：2010-06-08
申请号：US10567002
申请日：2004-08-02
申请人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
发明人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
IPC分类号： B01L3/02
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502738 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0677 , B33Y40/00 , B33Y80/00 , C12Q2523/109 , G01N1/40 , Y10T436/25 , Y10T436/25375
摘要： A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
摘要翻译： 微流体装置包括用于将含颗粒的液体样品输入到装置中的输入端口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含有颗粒的液体样品的颗粒与含有流体样品的颗粒的液体的第一部分空间分离。 压力执行器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的一部分重新组合。 该装置还可以包括从保留构件接收颗粒和液体的裂解室。 裂解室热裂解颗粒以释放其内容物。
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公开(公告)号：US20080050804A1
公开(公告)日：2008-02-28
申请号：US11929971
申请日：2007-10-30
申请人： Kalyan Handique , Gene Parunak
发明人： Kalyan Handique , Gene Parunak
CPC分类号： C12Q1/686 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502784 , B01L7/52 , B01L2200/0605 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/10 , B01L2200/143 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/1805 , B01L2300/1827 , B01L2400/0406 , B01L2400/0442 , B01L2400/0487 , B01L2400/0633 , B01L2400/0688 , B01L2400/0694 , B01L2400/084 , F04B19/006 , F04B19/24 , G01N1/4077 , G01N2035/1044 , Y10T436/11 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： The present invention relates to a system and method for moving samples, such as fluid, within a microfluidic system using a plurality of gas actuators for applying pressure at different locations within the microfluidic. The system includes a substrate which forms a fluid network through which fluid flows, and a plurality of gas actuators integral with the substrate. One such gas actuator is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator.
摘要翻译： 本发明涉及一种使用多个气体致动器在微流体系统内移动样品（例如流体）的系统和方法，用于在微流体内的不同位置处施加压力。 该系统包括形成流体流过的流体网络的基板，以及与基板一体化的多个气体致动器。 一个这样的气体致动器在第一位置处联接到网络，以提供气体压力以移动网络内的微流体样品。 另一气体致动器在第二位置处联接到网络，用于提供气体压力以进一步移动网络内微流体样品的至少一部分。 阀连接到微流体网络，使得当阀关闭时，阀将第二气体致动器与第一气体致动器基本隔离。
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8.发明授权Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids 有权用于从流体微流体样品中的细胞释放细胞内物质的方法和系统公开(公告)号：US07192557B2
公开(公告)日：2007-03-20
申请号：US10014519
申请日：2001-12-14
申请人： Betty Wu , Karthik Ganesan , Kalyan Handique , Gene Parunak
发明人： Betty Wu , Karthik Ganesan , Kalyan Handique , Gene Parunak
CPC分类号： G01N1/4077 , B01L3/50273 , B01L3/502738 , B01L3/502784 , B01L7/52 , B01L2200/0647 , B01L2200/10 , B01L2300/0681 , B01L2300/1805 , B01L2400/0442 , B01L2400/0487 , B01L2400/0633 , B01L2400/0688 , F04B19/006 , F04B19/24 , Y10T436/11 , Y10T436/25 , Y10T436/25375
摘要： The present invention relates to a microfluidic system for processing a cell-containing liquid. The system includes a lysing zone to receive the cell-containing sample and a positioning element to position the cell-containing sample in a lysing position in the vicinity of a lysing mechanism. The lysing mechanism releases intracellular material, such as DNA or RNA, from the cells. In one embodiment, the lysing mechanism includes electrodes for generating an electric field sufficient to release intracellular contents from the cells. Alternatively, the lysing mechanism may lyse the cells using chemical, heat and/or ultrasonic techniques or any combination of these techniques.
摘要翻译： 本发明涉及用于处理含细胞液体的微流体系统。 该系统包括用于接收含细胞的样品的裂解区和定位元件，以将含细胞的样品定位在裂解机制附近的裂解位置。 裂解机制从细胞中释放细胞内的物质，如DNA或RNA。 在一个实施方案中，裂解机制包括用于产生足以从细胞释放细胞内内容物的电场的电极。 或者，裂解机制可以使用化学，热和/或超声技术或这些技术的任何组合来裂解细胞。
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公开(公告)号：US20060205085A1
公开(公告)日：2006-09-14
申请号：US10567002
申请日：2004-08-02
申请人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
发明人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502738 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0677 , B33Y40/00 , B33Y80/00 , C12Q2523/109 , G01N1/40 , Y10T436/25 , Y10T436/25375
摘要： A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
摘要翻译： 微流体装置包括用于将含颗粒的液体样品输入到装置中的输入端口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含有颗粒的液体样品的颗粒与含有流体样品的颗粒的液体的第一部分空间分离。 压力执行器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的一部分重新组合。 该装置还可以包括从保留构件接收颗粒和液体的裂解室。 裂解室热裂解颗粒以释放其内容物。
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公开(公告)号：US08679831B2
公开(公告)日：2014-03-25
申请号：US12702648
申请日：2010-02-09
申请人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
发明人： Kalyan Handique , Gene Parunak , Aaron Kehrer , Betty Wu , Karthik Ganesan
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502738 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0677 , B33Y40/00 , B33Y80/00 , C12Q2523/109 , G01N1/40 , Y10T436/25 , Y10T436/25375
摘要： A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
摘要翻译： 微流体装置包括用于将含颗粒的液体样品输入到装置中的输入端口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含有颗粒的液体样品的颗粒与含有流体样品的颗粒的液体的第一部分空间分离。 压力执行器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的一部分重新组合。 该装置还可以包括从保留构件接收颗粒和液体的裂解室。 裂解室热裂解颗粒以释放其内容物。
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