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    Pipette-loaded bioassay assembly for detecting molecular or cellular events
    1.
    发明授权
    Pipette-loaded bioassay assembly for detecting molecular or cellular events 失效
    用于检测分子或细胞事件的移液器生物测定组件

    公开(公告)号：US06461808B1

    公开(公告)日：2002-10-08

    申请号：US09880746

    申请日：2001-06-12

    申请人： Kevin S. Bodner ,  Andrew P. Sandham

    发明人： Kevin S. Bodner ,  Andrew P. Sandham

    IPC分类号： C12Q100

    CPC分类号： G01N22/00 ,  G01N33/54373 ,  Y10T436/11 ,  Y10T436/119163

    摘要： A pipette-loaded bioassay includes a measurement probe and a pipette tip. The measurement probe includes a probe head configured to launch an incident test signal and a connecting end configured to receive the incident test signal from a signal source. The pipette tip includes a sample interrogation region which is electromagnetically coupled to the probe head, the sample interrogation region configured to retain a plug of sample solution and constructed from a material which is substantially transparent to the incident test signal. The incident test signal electromagnetically couples through the sample interrogation region and to the molecular or cellular events occurring within the sample solution. The interaction of the incident test signal with the molecular or cellular events produces a modulated test signal which can be recovered and used to identify the molecular or cellular events occurring in a subsequently tested sample.

    摘要翻译： 移液管装载的生物测定包括测量探针和移液管尖。 测量探头包括被配置为发射入射测试信号的探头和被配置为从信号源接收入射测试信号的连接端。 移液管尖端包括电磁耦合到探针头的样品询问区域，样品询问区域被配置为保持样品溶液塞并由对入射的测试信号基本透明的材料构成。 事件测试信号通过样品询问区域电磁耦合到样品溶液中发生的分子或细胞事件。 事件测试信号与分子或细胞事件的相互作用产生调制的测试信号，其可以被回收并用于鉴定随后测试的样品中发生的分子或细胞事件。

    Repuncturable self-sealing sample container with internal collapsible bag
    3.
    发明授权
    Repuncturable self-sealing sample container with internal collapsible bag 失效
    具有内部可折叠袋的可重复自密封样品容器

    公开(公告)号：US06805842B1

    公开(公告)日：2004-10-19

    申请号：US09976628

    申请日：2001-10-12

    申请人： Kevin S. Bodner ,  Tyler A. Palmer ,  Pejman Ghanouni

    发明人： Kevin S. Bodner ,  Tyler A. Palmer ,  Pejman Ghanouni

    IPC分类号： B01L300

    CPC分类号： B01L3/505 ,  B01L3/0217 ,  B01L3/50825 ,  B01L3/5085 ,  B01L2200/142 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/0672 ,  B01L2300/0854 ,  B01L2400/0478

    摘要： A sample container for minimizing evaporation of a contained volume of sample includes a container housing, a repuncturable self-sealing membrane, and a collapsible sample bag. The container housing includes an open end and a hollow interior region. The repuncturable self-sealing membrane configured to self-seal after repeated punctures is engaged in the open end of the container housing and includes an exterior surface exposed to the external environment and an interior surface oriented toward the hollow interior region of the container housing. The collapsible sample bag includes a proximate end that is permanently attached to the interior surface of the repuncturable self-sealing membrane.

    摘要翻译： 用于最小化容纳体积的样品的蒸发的样品容器包括容器壳体，可重复的自密封膜和可折叠样品袋。 容器壳体包括开口端和中空的内部区域。 构造为在重复穿刺之后自密封的可重复使用的自密封膜被接合在容器壳体的开口端中，并且包括暴露于外部环境的外表面和朝向容器壳体的中空内部区域定向的内表面。 可折叠的样品袋包括永久地附着到可再次自密封膜的内表面的近端。

    Purification apparatus and method
    5.
    发明授权
    Purification apparatus and method 失效
    净化装置及方法

    公开(公告)号：US06419827B1

    公开(公告)日：2002-07-16

    申请号：US09552301

    申请日：2000-04-18

    申请人： Donald R. Sandell ,  Kevin S. Bodner ,  Mark Borodkin ,  Mark Oldham ,  Jon Hoshizaki

    发明人： Donald R. Sandell ,  Kevin S. Bodner ,  Mark Borodkin ,  Mark Oldham ,  Jon Hoshizaki

    IPC分类号： B01D6300

    CPC分类号： B01L3/50255 ,  B01D61/18 ,  B01L3/5025 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102

    摘要： The invention includes a filtration apparatus for processing a plurality of fluid samples into sample wells is provided. In one embodiment, the filtration apparatus includes a purification tray and a sample well tray with a plurality of sample wells. The purification tray includes a filter plate having a plurality of columns with discharge openings at the bottom thereof, at least one filter positioned in the columns of the filter plate for filtering the fluid samples as they pass therethrough, a heat plate positioned adjacent the columns of the filter plate, and a vent plate positioned below the heat plate. The heat plate is configured for transferring heat to the columns of the filter plate. The vent plate includes vents for permitting aerosols from the sample wells of the sample well tray to escape. A sample well tray with a plurality of the sample wells is positioned so that the sample wells align with the discharge openings of the filter plate columns to receive the liquid sample therein.

    摘要翻译： 本发明提供了一种用于将多个流体样品加工成样品孔的过滤装置。 在一个实施例中，过滤装置包括净化盘和具有多个样品孔的样品井托盘。 净化盘包括一个过滤板，该过滤板具有多个在其底部具有排放口的柱，至少一个过滤器，定位在过滤板的列中，用于在流体样品通过过滤器时对其进行过滤， 过滤板和位于加热板下方的通风板。 加热板被配置为将热量传递到过滤板的列。 通风板包括用于允许来自样品井托盘的样品井的气溶胶逸出的通风口。 定位具有多个样品孔的样品孔盘，使得样品孔与过滤板柱的排出口对准以在其中接收液体样品。

    System for real time detection of nucleic acid amplification products
    6.
    发明授权
    System for real time detection of nucleic acid amplification products 失效
    核酸扩增产物实时检测系统

    公开(公告)号：US5928907A

    公开(公告)日：1999-07-27

    申请号：US752973

    申请日：1996-12-02

    申请人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    发明人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/03 ,  G01N21/64 ,  G01N21/75 ,  G01N21/78 ,  G01N33/50 ,  G01N33/58

    CPC分类号： G01N21/6428 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/0303 ,  G01N2021/6484

    摘要： A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.

    摘要翻译： 提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。 在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物含有（i）第一荧光指示剂，其能够产生第一荧光信号，其强度与扩增产物的量成比例 在由激发束照射的反应混合物的体积中和（ii）均匀分布在整个反应混合物中的第二荧光指示器，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。 优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。 相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。 第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。