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    SYSTEMS AND METHODS FOR EFFICIENT COLLECTION OF SINGLE CELLS AND COLONIES OF CELLS AND FAST GENERATION OF STABLE TRANSFECTANTS
    1.
    发明申请
    SYSTEMS AND METHODS FOR EFFICIENT COLLECTION OF SINGLE CELLS AND COLONIES OF CELLS AND FAST GENERATION OF STABLE TRANSFECTANTS 有权
    有效收集单细胞和细胞群的系统和方法以及快速生成稳定转录因子

    公开(公告)号：US20070238122A1

    公开(公告)日：2007-10-11

    申请号：US11733053

    申请日：2007-04-09

    申请人： Nancy Allbritton ,  Christopher Sims ,  Yuli Wang ,  Mark Bachman ,  Guann-Pyng Li ,  Eric Stanbridge

    发明人： Nancy Allbritton ,  Christopher Sims ,  Yuli Wang ,  Mark Bachman ,  Guann-Pyng Li ,  Eric Stanbridge

    IPC分类号： C12Q1/68 ,  C12N1/20

    CPC分类号： G01N1/286 ,  B01L3/5085 ,  B01L2200/0668 ,  B01L2300/0829 ,  C12M23/12 ,  C12M47/04 ,  G01N2001/2886

    摘要： A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses. Use of the plate advantageously enables the selection and sorting of cells based on dynamic phenomena and the rapid establishment of stable tranfectants.

    摘要翻译： 制造的板可使细胞，微生物，蛋白质，DNA，生物分子和其他生物培养基的样品定位在板上的特定位置或位点，以便对样品进行可寻址分析。 优选地，一些或所有的部位由可移除的材料或托盘构建，使得感兴趣的样品的子集可以容易地与板分离以用于进一步的处理或分析。 该板可以包含增强或促进样品的附着和/或功能的结构或化学处理，并且促进或辅助其分析。 板的使用有利于基于动态现象和快速建立稳定的转染剂来选择和分选细胞。

    MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS
    3.
    发明申请
    MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS 有权
    用于绘制生物和非生物材料的微孔板

    公开(公告)号：US20070128716A1

    公开(公告)日：2007-06-07

    申请号：US11539695

    申请日：2006-10-09

    申请人： Yuli Wang ,  Mark Bachman ,  Christopher Sims ,  Guann-Pyng Li ,  Nancy Allbritton

    发明人： Yuli Wang ,  Mark Bachman ,  Christopher Sims ,  Guann-Pyng Li ,  Nancy Allbritton

    IPC分类号： C12M1/22

    CPC分类号： B01L3/5085 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2200/12 ,  B01L2300/0819 ,  C12M25/06 ,  C12M29/20

    摘要： Systems and methods are provided for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiment of the present invention include a method for placing materials on the plate at pre-determined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate with coatings at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micro-mechanical structures—where the structures may be formed from living or non-living material, tissue or non-tissue, organic or inorganic, and the like.

    摘要翻译： 提供了系统和方法，用于在板上的特定位置图案化生物和非生物材料，以及生长三维结构。 优选实施例包括具有区域的板，当板被浸没在液体中时，其具有通常以气泡形式捕获气体的区域。 本发明的其它实施方案包括通过使用捕获的气体将材料放置在预定位置处的材料的方法，以防止材料在不需要的区域收集。 该板对于在特定位置（例如阵列）上的电镀细胞和组织具有很大的用途。 所公开的方法还可以用于在具有特定位置的涂层涂覆板的表面以用于图案化涂布应用，并且建立材料以产生三维结构，包括微机械结构，其中结构可以由活的或非 - 生物材料，组织或非组织，有机或无机物等。

    Micropatterned plate with micro-pallets for addressable biochemical analysis
    5.
    发明授权
    Micropatterned plate with micro-pallets for addressable biochemical analysis 有权
    具有微型托盘的微图案板，用于可寻址的生化分析

    公开(公告)号：US07695954B2

    公开(公告)日：2010-04-13

    申请号：US11243926

    申请日：2005-10-04

    申请人： Mark Bachman ,  Guann-Pyng Li ,  Nancy Allbritton ,  Christopher Sims

    发明人： Mark Bachman ,  Guann-Pyng Li ,  Nancy Allbritton ,  Christopher Sims

    IPC分类号： C12M1/34 ,  C12M3/00

    CPC分类号： B01L3/5027 ,  B01L3/5025 ,  B01L3/5085 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0454

    摘要： A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses.

    摘要翻译： 制造的板可使细胞，微生物，蛋白质，DNA，生物分子和其他生物培养基的样品定位在板上的特定位置或位点，以便对样品进行可寻址分析。 优选地，一些或所有的部位由可移除的材料或托盘构建，使得感兴趣的样品的子集可以容易地与板分离以用于进一步的处理或分析。 该板可以包含增强或促进样品的附着和/或功能的结构或化学处理，并且促进或辅助其分析。

    Micropatterned plate with micro-pallets for addressable biochemical analysis
    6.
    发明申请
    Micropatterned plate with micro-pallets for addressable biochemical analysis 有权
    具有微型托盘的微图案板，用于可寻址的生化分析

    公开(公告)号：US20060121500A1

    公开(公告)日：2006-06-08

    申请号：US11243926

    申请日：2005-10-04

    申请人： Mark Bachman ,  Guann-Pyng Li ,  Nancy Allbritton ,  Christopher Sims

    发明人： Mark Bachman ,  Guann-Pyng Li ,  Nancy Allbritton ,  Christopher Sims

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： B01L3/5027 ,  B01L3/5025 ,  B01L3/5085 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0454

    摘要： A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses.

    摘要翻译： 制造的板可使细胞，微生物，蛋白质，DNA，生物分子和其他生物培养基的样品定位在板上的特定位置或位点，以便对样品进行可寻址分析。 优选地，一些或所有的部位由可移除的材料或托盘构成，使得感兴趣的样品的子集可以容易地与板分离以用于进一步的处理或分析。 该板可以包含增强或促进样品的附着和/或功能的结构或化学处理，并且促进或辅助其分析。

    Array of micromolded structures for sorting adherent cells
    7.
    发明授权
    Array of micromolded structures for sorting adherent cells 有权
    用于分类贴壁细胞的微复合结构阵列

    公开(公告)号：US09068155B2

    公开(公告)日：2015-06-30

    申请号：US13513310

    申请日：2012-06-01

    申请人： Nancy Allbritton ,  Christopher Sims ,  Yuli Wang ,  Pavak Kirit Shah

    发明人： Nancy Allbritton ,  Christopher Sims ,  Yuli Wang ,  Pavak Kirit Shah

    IPC分类号： C40B50/06 ,  C40B60/14 ,  C12M1/32 ,  C12M1/12

    CPC分类号： C12M23/12 ,  B01J19/0046 ,  B01J2219/00452 ,  B01J2219/00743 ,  C12M23/20 ,  C12M23/22 ,  C12M25/04 ,  C12M25/16 ,  C12M47/02 ,  C12M47/04

    摘要： An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

    摘要翻译： 用于收集或培养细胞或细胞集落的装置包括：由柔性弹性聚合物材料形成且在其中形成有多个孔的共同基底; 以及可释放地连接到所述公共衬底的多个刚性电池载体，所述载体以阵列的形式布置，并且每个载体弹性地容纳在其中一个孔中。 使用这种装置收集或培养细胞或细胞集落的方法是通过在装置上沉积携带细胞的液体培养基进行的，以使所述细胞沉降或粘附到所述载体上; 然后（c）通过从其被接收的空腔（例如通过用探针推动）逐渐地向每个载体施加释放能量，从而释放至少一个具有所述细胞的选定载体。