公开(公告)号：US08759099B2

公开(公告)日：2014-06-24

申请号：US13808390

申请日：2011-07-13

申请人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myoui ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

发明人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myoui ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

IPC分类号： C12N5/00

CPC分类号： C12N5/0676 ,  C12N2501/734

摘要： The disclosed islet isolation method comprises: an injection step of injecting a preservation solution into the pancreatic duct of an excised pancreas; a preservation step of immersing the pancreas into an immersion fluid for preservation; a digestion step of breaking down the pancreas to provide pancreatic tissue; and a purification step of immersing the pancreatic tissue in a purification solution to provide islets. The digestion step consists of: an enzyme injection step of injecting an enzyme solution containing a digestion enzyme into the pancreas; a digestion initiation step of activating the digestion enzyme; a digestion termination step of inactivating the digestion enzyme; and a collection step of collecting the broken-down pancreatic tissue. The islet isolation method is characterized in that, by adding a neutrophil elastase inhibitor to the system before the digestion initiation step, the neutrophil elastase inhibitor is present inside the pancreas at the time point of starting the digestion initiation step. By using the above method and a protective solution which can be used in the method, islets having a size and shape suitable for transplantation can be obtained in high yields.

摘要翻译： 公开的胰岛分离方法包括：将保存液注入切除胰腺胰管的注射步骤; 将胰脏浸入用于保存的浸液中的保存步骤; 消化胰腺胰腺组织消化步骤; 以及将胰组织浸入净化溶液中以提供胰岛的纯化步骤。 消化步骤包括：将含有消化酶的酶溶液注入胰腺的酶注射步骤; 活化消化酶的消化引发步骤; 消化终止步骤，使消化酶失活; 以及收集分解的胰组织的收集步骤。 胰岛分离方法的特征在于，通过在消化引发步骤之前向系统中加入嗜中性粒细胞弹性蛋白酶抑制剂，在开始消化起始步骤的时间点，存在嗜中性粒细胞弹性蛋白酶抑制剂在胰腺内。 通过使用上述方法和可用于该方法的保护溶液，可以高产率获得具有适合于移植的尺寸和形状的胰岛。

公开(公告)号：US20130177986A1

公开(公告)日：2013-07-11

申请号：US13808390

申请日：2011-07-13

申请人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myouri ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

发明人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myouri ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

IPC分类号： C12N5/071

CPC分类号： C12N5/0676 ,  C12N2501/734

摘要： The disclosed islet isolation method comprises: an injection step of injecting a preservation solution into the pancreatic duct of an excised pancreas; a preservation step of immersing the pancreas into an immersion fluid for preservation; a digestion step of breaking down the pancreas to provide pancreatic tissue; and a purification step of immersing the pancreatic tissue in a purification solution to provide islets. The digestion step consists of: an enzyme injection step of injecting an enzyme solution containing a digestion enzyme into the pancreas; a digestion initiation step of activating the digestion enzyme; a digestion termination step of inactivating the digestion enzyme; and a collection step of collecting the broken-down pancreatic tissue. The islet isolation method is characterized in that, by adding a neutrophil elastase inhibitor to the system before the digestion initiation step, the neutrophil elastase inhibitor is present inside the pancreas at the time point of starting the digestion initiation step. By using the above method and a protective solution which can be used in the method, islets having a size and shape suitable for transplantation can be obtained in high yields.

摘要翻译： 公开的胰岛分离方法包括：将保存液注入切除胰腺胰管的注射步骤; 将胰脏浸入用于保存的浸液中的保存步骤; 消化胰腺胰腺组织消化步骤; 以及将胰组织浸入净化溶液中以提供胰岛的纯化步骤。 消化步骤包括：将含有消化酶的酶溶液注入胰腺的酶注射步骤; 活化消化酶的消化引发步骤; 消化终止步骤，使消化酶失活; 以及收集分解的胰组织的收集步骤。 胰岛分离方法的特征在于，通过在消化引发步骤之前向系统中加入嗜中性粒细胞弹性蛋白酶抑制剂，在开始消化起始步骤的时间点，存在嗜中性粒细胞弹性蛋白酶抑制剂在胰腺内。 通过使用上述方法和可用于该方法的保护溶液，可以高产率获得具有适合于移植的尺寸和形状的胰岛。

公开(公告)号：US20150032223A1

公开(公告)日：2015-01-29

申请号：US14008769

申请日：2012-04-02

申请人： Shigeru Miyagawa ,  Atsuhiro Saito ,  Yoshiki Sawa ,  Manabu Mizutani ,  Akima Harada

发明人： Shigeru Miyagawa ,  Atsuhiro Saito ,  Yoshiki Sawa ,  Manabu Mizutani ,  Akima Harada

IPC分类号： A61F2/02

CPC分类号： A61F2/02 ,  A61F2230/0006 ,  A61L27/38 ,  A61L27/52 ,  C12M25/02 ,  C12M33/00

摘要： A cell sheet transplantation device having a plane for transplanting a sheet of cultured cells, the device comprising, in the plane in the same direction, (1) a planar surface portion for capturing a cell sheet while maintaining a sheet-shaped form, and (2) suction holes for immobilizing a transplantation site by suction, the suction holes being positioned around the planar surface portion. By using the cell sheet transplantation device, a cultured cell sheet can be detached effectively, and the detached cultured cell sheet can be transplanted in an effective and simple manner.

摘要翻译： 一种具有用于移植培养细胞片的平面的细胞移植装置，所述装置在相同方向的平面中包括（1）用于在保持片状形状的同时捕获细胞片的平面部分，以及（ 2）用于通过抽吸固定移植部位的吸孔，吸孔位于平面部分周围。 通过使用细胞移植装置，可以有效地剥离培养的细胞片，并且可以有效且简单地移植分离的培养细胞片。

公开(公告)号：US20100068188A1

公开(公告)日：2010-03-18

申请号：US12311275

申请日：2007-09-20

申请人： Shinya Murakami ,  Tomoko Hashikawa ,  Yoshiki Sawa ,  Akifumi Matsuyama ,  Hiroshi Komoda

发明人： Shinya Murakami ,  Tomoko Hashikawa ,  Yoshiki Sawa ,  Akifumi Matsuyama ,  Hiroshi Komoda

IPC分类号： A61K45/00 ,  C12N5/071 ,  A61P1/02

CPC分类号： A61L27/3865 ,  A61K35/12 ,  A61L27/3834 ,  A61L27/3895 ,  A61L2430/12 ,  C12N5/0654 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2501/30 ,  C12N2501/39 ,  C12N2506/1384

摘要： An object of the present invention is to provide technology of using a preadipocyte to restore comprehensively bone, cartilage and periodontal ligament, and cause a periodontal hard tissue to regenerate. By culturing a preadipocyte using a culture medium containing (i) at least one member selected from the group consisting of glycerophosphate and salts thereof, and (ii) at least one member selected from the group consisting of ascorbic acid, derivatives thereof and salts thereof and not containing an adrenal cortical hormone in such an amount that promotes differentiation of a preadipocyte into a periodontal hard tissue cell, differentiation of the cell into a periodontal hard tissue cell is induced efficiently. Furthermore, a scaffold is combined with the preadipocyte cultured with the above-mentioned culture medium to be transplanted into a periodontal tissue that requires regeneration.

摘要翻译： 本发明的目的是提供使用前脂肪细胞全面恢复骨，软骨和牙周韧带并使牙周硬组织再生的技术。 通过使用培养基培养前脂肪细胞，所述培养基含有（i）选自甘油磷酸盐及其盐的至少一种，和（ii）至少一种选自抗坏血酸，其衍生物和盐及其盐的成员，以及 不含有促进前脂肪细胞分化为牙周硬组织细胞的量的肾上腺皮质激素，有效诱导细胞向牙周硬组织细胞的分化。 此外，将支架与与上述培养基一起培养的前脂肪细胞组合以移植到需要再生的牙周组织中。

公开(公告)号：US20090012498A1

公开(公告)日：2009-01-08

申请号：US11665007

申请日：2005-10-27

申请人： Yoshiki Sawa ,  Yukitoshi Shirakawa ,  Eisuke Tatsumi ,  Yoshiyuki Taenaka ,  Hikaru Matsuda

发明人： Yoshiki Sawa ,  Yukitoshi Shirakawa ,  Eisuke Tatsumi ,  Yoshiyuki Taenaka ,  Hikaru Matsuda

IPC分类号： A61M31/00 ,  A61N1/362

CPC分类号： A61K38/1833 ,  A61K48/005

摘要： The present invention provides a medicament comprising a gene encoding an angiogenic cytokine for the treatment of acute myocardial infarction (AMI), idiopathic cardiomyopathy (ICM), dilated cardiomyopathy (DCM) or heart failure, to be given in combination with ventricular assist device (VAD).

摘要翻译： 本发明提供了包含编码用于治疗急性心肌梗死（AMI），特发性心肌病（ICM），扩张性心肌病（DCM）或心力衰竭）的血管生成细胞因子的基因与心室辅助装置（VAD）组合的药物 ）。

公开(公告)号：US20070244568A1

公开(公告)日：2007-10-18

申请号：US10584740

申请日：2004-12-24

申请人： Hikaru Matsuda ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa ,  Shigemitau Iwai ,  Takeyoshi Ota ,  Jun Miyake ,  Masayuki Hara ,  Masakazu Furuta ,  Eiichiro Uchimura

发明人： Hikaru Matsuda ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa ,  Shigemitau Iwai ,  Takeyoshi Ota ,  Jun Miyake ,  Masayuki Hara ,  Masakazu Furuta ,  Eiichiro Uchimura

IPC分类号： A61F2/02

CPC分类号： A61L27/3683 ,  A61L27/3604

摘要： Decellularization of tissue by means of an amphipathic solvent a well-established practice. However, situations exist where the provision of enhanced decellularization is preferred. There is a demand for treating methods for coping with such situations. Thus, it is intended to provide a method for enhancing decellularization. The method comprises not only the immersing of a tissue in a solution containing an amphiphilic molecule in non-micellar form (for example, 1,2-epoxide polymer) but also performing a radical reaction (for example, treatment selected from the group consisting of exposure to gamma-ray irradiation, ultraviolet irradiation, a free radical supply source, ultrasonication, electron beam irradiation, and X-ray irradiation).

摘要翻译： 通过两性溶剂去除细胞组织，这是一个很好的做法。 然而，存在提供增强脱细胞化优先的情况。 需要处理这种情况的方法。 因此，旨在提供一种增强脱细胞化的方法。 该方法不仅包括将组织浸入含有非胶束形式的两亲分子（例如1,2-环氧化物聚合物）的溶液中，而且还进行自由基反应（例如，选自由 暴露于γ射线照射，紫外线照射，自由基供应源，超声波处理，电子束照射和X射线照射）。

公开(公告)号：US20070207540A1

公开(公告)日：2007-09-06

申请号：US11514468

申请日：2006-09-01

申请人： Mitsuru Akashi ,  Yoshiki Sawa ,  Michiya Matsusaki ,  Yoshio Nakahara

发明人： Mitsuru Akashi ,  Yoshiki Sawa ,  Michiya Matsusaki ,  Yoshio Nakahara

IPC分类号： C12N5/06

CPC分类号： C12N5/0068 ,  C12N5/0062 ,  C12N2533/52 ,  C12N2533/54 ,  Y10S977/902

摘要： The present invention provides a novel method of producing a three-dimensional tissue by which cell lamination can be carried out easily. According to the method, a three-dimensional tissue in which cell layers are laminated with an extracellular matrix intervening between each pair of the adjacent cell layers is produced by: (A) forming a cell layer on a substrate; (B) bringing the cell layer formed on the substrate into contact with a solution containing a first substance and a solution containing a second substance alternately, thus forming, on the cell layer, an extracellular matrix in which the first substance and the second substance are laminated alternately; and (C) culturing a cell on the extracellular matrix to form a further cell layer. In the present invention, a combination of the first substance and the second substance is (a) a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence or (b) a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.

摘要翻译： 本发明提供一种可以容易地进行细胞层压的三维组织的制造方法。 根据该方法，通过以下方法制造细胞层与介于每对相邻细胞层之间的细胞外基质层叠的三维组织：（A）在基材上形成细胞层; （B）使形成在基板上的细胞层与含有第一物质的溶液和含有第二物质的溶液交替接触，从而在细胞层上形成第一物质和第二物质为细胞外基质 交替叠层; 和（C）在细胞外基质上培养细胞以形成另外的细胞层。 在本发明中，第一物质和第二物质的组合是（a）具有RGD序列的蛋白质或聚合物和与具有RGD序列的蛋白质或聚合物相互作用的蛋白质或聚合物的组合，或（b） 带正电荷的蛋白质或聚合物和带负电荷的蛋白质或聚合物的组合。

公开(公告)号：US20070140914A1

公开(公告)日：2007-06-21

申请号：US11314493

申请日：2005-12-21

申请人： Yoshiki Sawa ,  Satoshi Taketani ,  Yoshiho Toyota ,  Shinji Ozawa ,  Nobuyoshi Watanabe

发明人： Yoshiki Sawa ,  Satoshi Taketani ,  Yoshiho Toyota ,  Shinji Ozawa ,  Nobuyoshi Watanabe

IPC分类号： B01L3/02

CPC分类号： A61M25/1002 ,  A61M25/0026 ,  A61M25/0084

摘要： The invention provides a reagent-introducing medical device that can guide a reagent into a reagent injector while maintaining all biological materials contained in the reagent in a healthy state. In an embodiment of the reagent-introducing medical device, an apparatus body (12) comprises a channel (77, 78, 80, 50e) through which a reagent flows, as well as a reagent inlet (76) and outlet (21), and further comprises a first control mechanism (58, 64) for controlling the flow of the reagent to create a laminar reagent flow in the channel (77, 78, 80, 50e) and a second control mechanism (70) for further controlling the laminar reagent flow in the channel to create a rotational reagent flow.

摘要翻译： 本发明提供了一种试剂导入医疗装置，其能够将试剂引导到试剂注射器中，同时将包含在试剂中的所有生物材料保持在健康状态。 在试剂导入医疗装置的一个实施方案中，装置主体（12）包括试剂流过的通道（77,78,80,450e）以及试剂入口（76）和出口（21）， ，还包括用于控制所述试剂的流动以在所述通道（77,78,80,450e）中产生层流试剂流的第一控制机构（58,64）和用于进一步控制的第二控制机构（70） 通道中的层流试剂流动以产生旋转试剂流。

公开(公告)号：US09845458B2

公开(公告)日：2017-12-19

申请号：US12664343

申请日：2008-06-16

申请人： Akifumi Matsuyama ,  Hiroshi Komoda ,  Hanayuki Ohkura ,  Yoshiki Sawa

发明人： Akifumi Matsuyama ,  Hiroshi Komoda ,  Hanayuki Ohkura ,  Yoshiki Sawa

IPC分类号： C12N5/00 ,  C12N5/0775

CPC分类号： C12N5/0667

摘要： Disclosed is a cell mass containing an adipose-tissue-derived multipotent progenitor cell. Also disclosed is a method for producing an adipose-tissue-derived multipotent progenitor cell from an adipose tissue, which comprises the steps of: (a) removing erythrocytes from an adipose-tissue-derived cell mass to produce a preadipose-tissue-derived multipotent progenitor cell mass; and (b) removing cells other than the adipose-tissue-derived multipotent progenitor cell from the preadipose-tissue-derived multipotent progenitor cell mass to produce the desired adipose-tissue-derived multipotent progenitor cell. Further disclosed is an adipose-tissue-derived multipotent progenitor cell produced by the method.

公开(公告)号：US09587222B2

公开(公告)日：2017-03-07

申请号：US10567728

申请日：2004-02-02

申请人： Hikaru Matsuda ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa

发明人： Hikaru Matsuda ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa

IPC分类号： C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C12N5/077 ,  A61L27/38 ,  A61K35/34 ,  A61K35/12

CPC分类号： C12N5/0658 ,  A61K35/12 ,  A61K35/34 ,  A61L27/3804 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3895 ,  A61L2430/20 ,  C12N5/0068 ,  C12N5/0657 ,  C12N2506/02 ,  C12N2506/1392 ,  C12N2513/00 ,  C12N2523/00 ,  C12N2533/40 ,  C12N2539/10

摘要： The present invention provides a prosthetic tissue or sheet capable of withstanding implantation operations, which can be used in actual operation and can be produced by culture. The present invention also provides a novel therapy which can substitute for cell therapy. Particularly, the present invention provides a method for producing a prosthetic tissue comprising a cell derived from a part other than myocardium and capable of withstanding implantation operation. The above-described objects of the present invention were partially achieved by finding that by culturing cells under specific culture conditions, the cells are unexpectedly organized into a tissue, and the resultant prosthetic tissue is capable of being detached from culture dishes. The present invention also provides a three-dimensional structure applicable to heart, comprising a cell derived from a part other than the myocardium of an adult.

摘要翻译： 本发明提供能够承受植入操作的假体组织或片材，其可以在实际操作中使用并且可以通过培养产生。 本发明还提供了可代替细胞治疗的新型治疗方法。 特别地，本发明提供了一种用于制造假体组织的方法，所述假体组织包含源于除了心肌之外的部分的细胞并且能够承受植入操作。 通过发现通过在特定培养条件下培养细胞，细胞意外地组织成组织，并且所得到的假体组织能够从培养皿中分离，部分实现了本发明的上述目的。 本发明还提供了适用于心脏的三维结构，其包含源自成人心肌之外的部分的细胞。