公开(公告)号：US12104161B2

公开(公告)日：2024-10-01

申请号：US17371449

申请日：2021-07-09

申请人： Fina BioSolutions, LLC

发明人： Min-Ju Chang ,  Natalia Oganesyan ,  Andrew Lees

IPC分类号： C12N15/70 ,  A61K38/00 ,  A61K47/60 ,  C07K1/22 ,  C07K14/235 ,  C07K14/33 ,  C07K14/34 ,  C12N9/02 ,  C12N9/06

CPC分类号： C12N15/70 ,  A61K47/60 ,  C07K1/22 ,  C07K14/235 ,  C07K14/33 ,  C07K14/34 ,  C12N9/003 ,  C12N9/0051 ,  A61K38/00 ,  C12N2830/30

摘要： The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5′-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.

公开(公告)号：US12098169B2

公开(公告)日：2024-09-24

申请号：US16651720

申请日：2018-09-28

申请人： Children's Medical Center Corporation ,  Paul Stenmark

发明人： Min Dong ,  Paul Stenmark ,  Sicai Zhang ,  Andrew Doxey

IPC分类号： C07K14/315 ,  A61K38/00 ,  C07K14/00 ,  C07K14/195 ,  C07K14/33

CPC分类号： C07K14/315 ,  C07K14/33 ,  A61K38/00 ,  C07K2319/50

摘要： Provided herein is a novel homolog of Clostridial botulinum neurotoxin (BoNT) identified in the bacterial species Enterococcus faecium (BoNT/EN). BoNT/EN variants and chimeric toxins comprising domains from BoNT/EN and other BoNTs are also provided. Further provided herein are methods of producing BoNT/EN and its use, e.g., in therapeutic applications.

公开(公告)号：US11952601B2

公开(公告)日：2024-04-09

申请号：US16498193

申请日：2017-06-20

申请人： MERZ PHARMA GMBH & CO. KGAA

发明人： Fred Hofmann ,  Marcel Jurk ,  Manuela López De La Paz ,  Daniel Scheps ,  Jürgen Frevert

IPC分类号： C07K14/33 ,  A61K8/66 ,  A61K38/16 ,  C12N9/52 ,  C12N15/52 ,  C12N15/70 ,  A61K38/00

CPC分类号： C12N9/52 ,  A61K8/66 ,  C12Y304/24069 ,  A61K38/00 ,  C07K2319/31

摘要： This invention relates to novel recombinant clostridial neurotoxins exhibiting an increased duration of effect without a delayed onset of effect and to methods for the manufacture of such recombinant clostridial neurotoxins. These novel recombinant clostridial neurotoxins comprise a domain consisting of proline, alanine, serine, threonine, glycine and glutamate residues, and the methods comprise the steps of inserting a nucleic acid sequence coding for said domain into a nucleic acid sequence coding for a parental clostridial neurotoxin and expression of the recombinant nucleic acid sequence comprising said domain-coding sequence in a host cell. The invention further relates to novel recombinant single-chain precursor clostridial neurotoxins used in such methods, nucleic acid sequences encoding such recombinant single-chain precursor clostridial neurotoxins, and pharmaceutical compositions comprising the recombinant clostridial neurotoxin with an increased duration of effect without a delayed onset of effect.

公开(公告)号：US11952597B2

公开(公告)日：2024-04-09

申请号：US16922952

申请日：2020-07-07

申请人： Pfizer Inc.

发明人： Jason Arnold Lotvin ,  Annaliesa Sybil Anderson ,  Robert G. K. Donald ,  Michael James Flint ,  Narender Kumar Kalyan ,  Kathrin Ute Jansen ,  Maninder K. Sidhu ,  Justin Keith Moran ,  Mark Edward Ruppen ,  Weiqiang Sun

IPC分类号： C12N9/10 ,  C07K14/33 ,  C12N1/20

CPC分类号： C12N9/1051 ,  C07K14/33 ,  C12N1/20

摘要： In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. The mutant toxin may include a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to methods and compositions for use in culturing Clostridium difficile and in producing C. difficile toxins.

公开(公告)号：US11951165B2

公开(公告)日：2024-04-09

申请号：US16459303

申请日：2019-07-01

申请人： Vaxcyte, Inc.

发明人： Jeffery C. Fairman ,  Jon H. Heinrichs ,  Wei Chan

IPC分类号： A61K39/385 ,  A61K39/09 ,  A61P31/04 ,  C07K14/00 ,  C07K14/285 ,  C07K14/33 ,  C07K14/34 ,  A61K38/00 ,  A61K39/00

CPC分类号： A61K39/385 ,  A61K39/092 ,  A61P31/04 ,  C07K14/001 ,  C07K14/285 ,  C07K14/33 ,  C07K14/34 ,  A61K38/00 ,  A61K2039/6037 ,  A61K2039/627 ,  A61K2039/70

摘要： Methods for the production of immunogenic compositions containing a non-natural amino acid are disclosed. The non-natural amino acid can be a site for attachment of antigens, such as bacterial capsular polysaccharides, to make immunogenic conjugates. Bio-orthogonal attachment chemistry incorporated into the non-natural amino acids allows for more efficient and potent antigen presentation to the immune system, simplified purification, and more well-defined structure of these semi-synthetic immunogens.

公开(公告)号：US20240076325A1

公开(公告)日：2024-03-07

申请号：US18340479

申请日：2023-06-23

申请人： Evaxion Biotech A/S

发明人： Niels Iversen Møller ,  Andreas Holm Mattsson

IPC分类号： C07K14/21 ,  A61K39/104 ,  A61P31/04 ,  C07K14/33 ,  C07K14/34 ,  C07K14/435 ,  C07K16/12 ,  C12Q1/689 ,  G01N33/569

CPC分类号： C07K14/21 ,  A61K39/104 ,  A61P31/04 ,  C07K14/33 ,  C07K14/34 ,  C07K14/43504 ,  C07K16/1214 ,  C12Q1/689 ,  G01N33/56911 ,  A61K2039/505

摘要： Immunogenic proteins from Pseudomonas aeruginosa as well as nucleic acids, vectors and transformed cells useful for expression of the proteins. Also disclosed are methods for prophylaxis of infection with Pseudomonas aeruginosa using the proteins, nucleic acids, vectors or transformed cel

公开(公告)号：US11911449B2

公开(公告)日：2024-02-27

申请号：US17322901

申请日：2021-05-18

申请人： REVANCE THERAPEUTICS, INC.

发明人： Stewart A. Thompson ,  Curtis L. Ruegg ,  Jacob M. Waugh

IPC分类号： A61K38/48 ,  A61K8/49 ,  A61K8/60 ,  A61K8/64 ,  A61K39/08 ,  A61K8/02 ,  A61K8/66 ,  A61K8/84 ,  A61K9/14 ,  A61K9/19 ,  A61K47/22 ,  A61K47/26 ,  A61K47/34 ,  A61K47/42 ,  A61Q19/00 ,  A61Q19/08 ,  C07K14/33 ,  A61K8/90

CPC分类号： A61K38/4893 ,  A61K8/022 ,  A61K8/4946 ,  A61K8/4973 ,  A61K8/4993 ,  A61K8/60 ,  A61K8/602 ,  A61K8/64 ,  A61K8/66 ,  A61K8/84 ,  A61K8/90 ,  A61K9/14 ,  A61K9/19 ,  A61K39/08 ,  A61K47/22 ,  A61K47/26 ,  A61K47/34 ,  A61K47/42 ,  A61Q19/008 ,  A61Q19/08 ,  C07K14/33 ,  C12Y304/24069 ,  Y02A50/30

摘要： This invention relates to botulinum toxin formulations that are stabilized without the use of any proteinaceous excipients. The invention also relates to methods of preparing and using such botulinum toxin formulations.

公开(公告)号：US20240058438A1

公开(公告)日：2024-02-22

申请号：US18099035

申请日：2023-01-19

申请人： The Governors of the University of Alberta ,  Helmholtz Center for Infection Research

发明人： Michael Houghton ,  Abdolamir Landi ,  Carlos A. Guzman ,  Thomas Ebensen ,  Darren Hockman ,  John L. Law ,  Michael Logan

IPC分类号： A61K39/29 ,  A61P31/14 ,  A61P37/04 ,  A61K39/12 ,  C07K14/005 ,  A61K39/39 ,  C01F11/02 ,  C07D323/00 ,  C07K14/18 ,  C07K14/28 ,  C07K14/33 ,  C07K14/34 ,  C12N15/86

CPC分类号： A61K39/29 ,  A61P31/14 ,  A61P37/04 ,  A61K39/12 ,  C07K14/005 ,  A61K39/39 ,  C01F11/02 ,  C07D323/00 ,  C07K14/18 ,  C07K14/28 ,  C07K14/33 ,  C07K14/34 ,  C12N15/86 ,  A61K2039/543

摘要： The present disclosure provides immunogenic compositions comprising: a) hepatitis C virus (HCV) E1E2 heterodimers, HCV E2, or HCV E1; and b) an adjuvant, where the adjuvant is a cyclic dinucleotide or an archaeosome. The present disclosure provides methods of inducing an immune response in an individual to HCV, the methods comprising administering to an individual an effective amount of an immunogenic composition of the present disclosure.

公开(公告)号：US11773145B2

公开(公告)日：2023-10-03

申请号：US16632299

申请日：2018-07-20

申请人： ShanghaiTech University

发明人： Jia Liu ,  Biao Jiang

IPC分类号： C07K14/33 ,  C07K19/00 ,  A61K38/00

CPC分类号： C07K14/33 ,  C07K19/00 ,  A61K38/00 ,  C07K2319/55 ,  C07K2319/81

摘要： Provided are chimeric polypeptides that include one or more zinc finger motifs fused to a therapeutic peptide such as botulinum neurotoxins (BoNTs). The zinc finger motif may be located at the C-terminal side of the BoNT and the chimeric polypeptide can optionally include two or more such zinc finger motifs. It said the disclosed chimeric polypeptides can be efficiently delivered to a subject transdermally.

公开(公告)号：US20230305002A1

公开(公告)日：2023-09-28

申请号：US18330411

申请日：2023-06-07

申请人： Massachusetts Institute of Technology

发明人： Hadley Sikes Johnson ,  Eric Alexander Miller ,  Ki-Joo Sung

IPC分类号： G01N33/566 ,  C07K14/195 ,  C07K14/33 ,  C07K16/06 ,  C07K16/28 ,  G01N33/569

CPC分类号： G01N33/566 ,  C07K14/195 ,  C07K14/33 ,  C07K16/065 ,  C07K16/28 ,  G01N33/56911 ,  C07K2319/20 ,  G01N2400/26 ,  C07K2319/24 ,  C07K2319/60

摘要： The present disclosure relates to proteins comprising a target-binding domain for detection of a target of interest, methods, compositions and kits thereof.