公开(公告)号：US09815061B2

公开(公告)日：2017-11-14

申请号：US14549113

申请日：2014-11-20

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Cyril Delattre ,  Arnaud Rival ,  Vijay Srinivasan

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/5029 ,  B01L3/52 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0442 ,  B01L2400/0448

Abstract: During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

公开(公告)号：US20170266653A1

公开(公告)日：2017-09-21

申请号：US15614944

申请日：2017-06-06

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. POLLACK ,  Ramakrishna SISTA ,  Zhishan HUA ,  Vamsee K. PAMULA ,  Arjun SUDARSAN ,  Vijay SRINIVASAN ,  Prasanna THWAR ,  Alexander SHENDEROV ,  Dwayne ALLEN

IPC: B01L3/02 ,  B03C1/28 ,  B01F13/08 ,  B01L3/00 ,  B01F13/00 ,  B03C1/01

CPC classification number: B01F13/0023 ,  B01F13/002 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0818 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0631 ,  B01L2200/0657 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/043 ,  B01L2400/0478 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/284 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  Y10T436/25

Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.

公开(公告)号：US09574220B2

公开(公告)日：2017-02-21

申请号：US14644989

申请日：2015-03-11

Applicant: Advanced Liquid Logic, Inc.

Inventor： Allen E Eckhardt ,  Carrie Graham ,  Ramakrishna Sista ,  Vamsee K. Pamula ,  Theodore Winger ,  Tong Wang ,  Yalin Xiong ,  Ning Wu ,  Sanjay Saha ,  Gajendrasinh Raolji ,  Raveendra Dayam

IPC: C12Q1/54 ,  C12Q1/34 ,  C07D311/18 ,  C07D311/20 ,  C07H15/203 ,  C12Q1/25 ,  G01N33/543 ,  G01N21/64 ,  C07D311/16 ,  C07D405/12 ,  C07D413/12 ,  C07H17/075 ,  C12Q1/44 ,  B01L3/00

CPC classification number: C12Q1/40 ,  B01L3/502784 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07D311/16 ,  C07D311/18 ,  C07D311/20 ,  C07D405/12 ,  C07D413/12 ,  C07H15/203 ,  C07H17/075 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q1/54 ,  C12Y302/01023 ,  G01N21/6452 ,  G01N33/5438 ,  G01N2333/916 ,  G01N2333/926 ,  G01N2333/938 ,  G01N2800/04

Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.

Abstract translation: 本发明涉及液滴致动器装置和测定方法。 本发明包括进行测定的测定方法，其包括将样品与伞形酮基衍生物组合，其中所述样品潜在地包含能够切割伞形变体基衍生物的酶，并且其中伞形载体衍生物包含用一个或多个修饰部分修饰的伞形酮核心。

公开(公告)号：US20160318021A1

公开(公告)日：2016-11-03

申请号：US15206860

申请日：2016-07-11

Applicant: Advanced Liquid Logic Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Philip Paik

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/0241 ,  B01L3/50273 ,  B01L2200/06 ,  B01L2300/06 ,  B01L2400/0427 ,  B41J2/14

Abstract: A method of manipulating a droplet comprising providing a substrate comprising a surface; an elongated transport electrode disposed on the substrate surface, the elongated transport electrode having a first and a second end and configured to impart a gradient force to the droplet; and one or more wires for providing power to the transport electrode; and providing power to the one or more wires to effect the gradient force and thereby transport the droplet along the length of the elongated transport electrode from the first end to the second end.

公开(公告)号：US09452433B2

公开(公告)日：2016-09-27

申请号：US14290057

申请日：2014-05-29

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Alexander Shenderov ,  Michael G. Pollack

IPC: B01L7/00 ,  B01L3/00

CPC classification number: B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.

公开(公告)号：US09377455B2

公开(公告)日：2016-06-28

申请号：US14746276

申请日：2015-06-22

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  G01N1/38 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US20160074863A1

公开(公告)日：2016-03-17

申请号：US14942125

申请日：2015-11-16

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Ryan A. Sturmer ,  Vijay Srinivasan ,  Arjun Sudarsan

IPC: B01L3/00 ,  G01N27/04

CPC classification number: B01L3/50273 ,  B01L3/502792 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0427 ,  G01N27/048 ,  G01R27/16

Abstract: Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.

Abstract translation: 方法包括测量由激发信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。 还公开了存储用于执行该方法的处理器可执行指令的计算机可读介质，以及系统。 系统包括存储在存储器中的处理器，存储器和代码，当执行时，处理器至少要使接收输出电压信号，将激励信号叠加在输出电压信号上，产生叠加信号，将叠加的信号连接到 液滴致动器中的电极，当检测到电极的阻抗时抑制输出电压信号，并测量由激励信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。

公开(公告)号：US20160068901A1

公开(公告)日：2016-03-10

申请号：US14888137

申请日：2014-05-01

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Allen E. Eckhardt ,  Michael G. Pollack ,  David S. Cohen

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01L7/52 ,  C12Q1/6844 ,  C40B20/00 ,  C12Q2533/101 ,  C12Q2565/301

Abstract: The invention provides pyrosequencing-based methods of analyzing and synthesizing DNA, including methods of DNA error correction, determining DNA size distribution, screening for nucleotide repeat disorders such as fragile X syndrome, determining size distribution and bias in a DNA library, and determining pyrosequencing read length. The methods include on-bench protocols as well as droplet-based protocols that may be conducted on a droplet actuator.

Abstract translation: 本发明提供了基于焦磷酸测序的DNA分析和合成方法，包括DNA错误校正方法，确定DNA大小分布，筛选核苷酸重复序列如脆性X综合征，确定DNA文库中的大小分布和偏差，以及确定焦磷酸测序读数 长度。 这些方法包括工作台协议以及可以在液滴致动器上进行的基于液滴的协议。

公开(公告)号：US20160016172A1

公开(公告)日：2016-01-21

申请号：US14837133

申请日：2015-08-27

Applicant: ADVANCED LIQUID LOGIC, INC. ,  DUKE UNIVERSITY

Inventor： Michael G. Pollack ,  Philip Paik ,  Vamsee K. Pamula

IPC: B01L7/00 ,  G01N27/447

CPC classification number: C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

Abstract: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.

Abstract translation: 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由其控制的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。

公开(公告)号：US09139865B2

公开(公告)日：2015-09-22

申请号：US13680979

申请日：2012-11-19

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Michael G. Pollack ,  Philip Paik ,  Vamsee K. Pamula

IPC: G05D5/06 ,  C12Q1/68 ,  G01N27/447

CPC classification number: C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

Abstract: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.

Abstract translation: 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由控制器执行指令的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。