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    METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS
    93.
    发明申请
    METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS 有权
    使用大规模FET阵列测量分析仪的方法和装置

    公开(公告)号：US20110217697A1

    公开(公告)日：2011-09-08

    申请号：US13001182

    申请日：2009-06-25

    Applicant: Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

    Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

    Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个示例中，chemFET阵列基于监测无机焦磷酸盐（PPi），氢离子和核苷酸三磷酸盐的浓度变化来促进DNA测序技术。

    Detection and confirmation of nucleic acid sequences by use of poisoning oligonucleotides
    96.
    发明授权
    Detection and confirmation of nucleic acid sequences by use of poisoning oligonucleotides 失效
    通过使用中毒寡核苷酸检测和确认核酸序列

    公开(公告)号：US06673577B1

    公开(公告)日：2004-01-06

    申请号：US09712018

    申请日：2000-11-14

    Applicant: Jonathan M. Rothberg ,  Michael W. Deem ,  John W. Simpson

    Inventor： Jonathan M. Rothberg ,  Michael W. Deem ,  John W. Simpson

    IPC: C12P1934

    CPC classification number: C12N15/1093 ,  C12Q1/6813 ,  C12Q1/6855

    Abstract: The present invention discloses a methodology which is directed to providing positive confirmation that nucleic acids, possessing putatively identified sequences predicted to generate observed GeneCalling™ signals, are actually present within the sample from which the signal was originally derived. The putatively identified nucleic acid fragment within the sample possesses 3′- and 5′-ends with known terminal subsequences. The method involves contacting nucleic acid fragments in a sample in amplifying conditions with (i) a nucleic acid polymerase; (ii) “regular” primer oligonucleotides having sequences comprising hybridizable portions of known terminal subsequences; and (iii) a “poisoning” oligonucleotide primer. Nucleic acids amplified with a poisoning primer are distinguishable upon detection from nucleic acids amplified with regular primers.

    Abstract translation: 本发明公开了一种旨在提供肯定确认的方法，核酸具有预测产生观察到的GeneCalling TM信号的推测鉴定的序列实际上存在于最初得到该信号的样本内。 样品中推测鉴定的核酸片段具有已知末端亚序列的3'-和5'-末端。 该方法包括在扩增条件下使样品中的核酸片段与（i）核酸聚合酶接触; （ii）具有包括已知末端亚序列的可杂交部分的序列的“常规”引物寡核苷酸; 和（iii）“中毒”寡核苷酸引物。 使用中毒引物扩增的核酸在检测到用常规引物扩增的核酸时是可区分的。

    Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
    97.
    发明授权
    Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments 失效
    用于产生，分离，检测和识别生物聚合物片段的装置和方法

    公开(公告)号：US06485625B1

    公开(公告)日：2002-11-26

    申请号：US09336848

    申请日：1999-06-21

    Applicant: John W. Simpson ,  Jonathan M. Rothberg ,  Gregory T. Went ,  Marie Carmen Ruiz-Martinez ,  Gregory T. Mulhern

    Inventor： John W. Simpson ,  Jonathan M. Rothberg ,  Gregory T. Went ,  Marie Carmen Ruiz-Martinez ,  Gregory T. Mulhern

    IPC: G01N2326

    CPC classification number: B01J19/0093 ,  G01N27/44708 ,  G01N27/44721 ,  G01N27/44782 ,  G01N27/44791

    Abstract: This invention is an integrated instrument for the high-capacity electrophoretic analysis of biopolymer samples. It comprises a specialized high-voltage, electrophoretic module in which the migration lanes are formed between a bottom plate and a plurality of etched grooves in a top plate, the module permitting concurrent separation of 80 or more separate samples. In thermal contact with the bottom plate is a thermal control module incorporating a plurality of Peltier heat transfer devices for the control of temperature and gradients in the electrophoretic medium. Fragments are detected by a transmission imaging spectrograph which simultaneously spatially focuses and spectrally resolves the detection region of all the migration lanes. The spectrograph comprises a transmission dispersion element and a CCD array to detect signals. Signal analysis comprises the steps of noise filtering, comparison in a configuration space with signal prototypes, and selection of the best prototype. Optionally post-processing is done by a Monte-Carlo simulated annealing algorithm to improve results. Optionally, an array of micro-reactors can be integrated into the instrument for the generation of sequencing reaction fragments directly from crude DNA samples.

    Abstract translation: 本发明是用于生物聚合物样品的高容量电泳分析的综合仪器。 它包括专门的高压电泳模块，其中迁移通道形成在顶板和顶板中的多个蚀刻凹槽之间，该模块允许同时分离80个或更多个单独的样品。 与底板热接触的是热控制模块，其包含多个用于控制电泳介质中的温度和梯度的珀尔帖传热装置。 通过透射成像光谱仪检测片段，其同时空间聚焦并光谱地解析所有迁移通道的检测区域。 该光谱仪包括用于检测信号的透射色散元件和CCD阵列。 信号分析包括噪声滤波，配置空间与信号原型的比较以及最佳原型的选择。 可选地，后处理通过蒙特卡罗模拟退火算法完成以改善结果。 任选地，可以将微反应器阵列整合到仪器中，以直接从粗DNA样品产生测序反应片段。

    Separation of charged particles by a spatially and temporally varying electric field
    98.
    发明授权
    Separation of charged particles by a spatially and temporally varying electric field 失效
    通过空间和时间变化的电场分离带电粒子

    公开(公告)号：US06193866B1

    公开(公告)日：2001-02-27

    申请号：US09212622

    申请日：1998-12-16

    Applicant: Joel S. Bader ,  Jonathan M. Rothberg ,  Michael W. Deem ,  Gregory T. Mulhern ,  Gregory T. Went ,  John Simpson ,  Steven Henck

    Inventor： Joel S. Bader ,  Jonathan M. Rothberg ,  Michael W. Deem ,  Gregory T. Mulhern ,  Gregory T. Went ,  John Simpson ,  Steven Henck

    IPC: G01N2726

    CPC classification number: B82Y30/00 ,  G01N27/44773

    Abstract: This invention relates to a method and device for separating charged particles according to their diffusivities in a separation medium by means of a spatially and temporally varying electric potential. The method is particularly suited to sizing and separating DNA fragments, to generating DNA fragment length polymorphism patterns, and to sequencing DNA through the separation of DNA sequencing reaction products. The method takes advantage of the transport of charged particles subject to an electric potential that is cycled between an off-state (in which the potential is flat) and one or more on-states, in which the potential is preferably spatially periodic with a plurality of eccentrically shaped stationary potential wells. The potential wells are at constant spatial positions in the on-state. Differences in liquid-phase diffusivities lead to charged particle separation. A preferred embodiment of the device is microfabricated. A separation medium fills physically defined separation lanes in the device. Electrodes deposited substantially transverse to the lanes create the required electric potentials. Advantageously, injection ports allow sample loading, and special gating electrodes focus the sample prior to separation. The effects of thermal gradients are minimized by placing the device in contact with a thermal control module, preferably a plurality of Peltier-effect heat transfer devices. The small size of a microfabricated device permits rapid separation in a plurality of separation lanes.

    Abstract translation: 本发明涉及一种通过空间和时间上变化的电势根据其在分离介质中的扩散性分离带电粒子的方法和装置。 该方法特别适用于分选DNA片段，产生DNA片段长度多态性模式，并通过分离DNA测序反应产物对DNA进行测序。 该方法利用带电粒子的传输，该带电粒子经历在关闭状态（其中电位为平坦）和一个或多个导通状态之间循环的电位，其中电位优选为空间周期性的多个 偏心固定势阱。 势阱在导通状态下处于恒定的空间位置。 液相扩散性的差异导致带电粒子分离。 装置的优选实施例是微制造的。 分离介质填充设备中物理定义的分离通道。 基本横向于通道沉积的电极产生所需的电位。 有利地，注射端口允许样品加载，并且特殊门电极在分离之前聚焦样品。 通过将设备与热控制模块（优选多个珀耳帖效应传热装置）接触来使热梯度的影响最小化。 微型加工装置的小尺寸允许在多个分离通道中快速分离。

    METHODS AND APPARATUSES FOR IDENTIFYING GESTURES BASED ON ULTRASOUND DATA
    100.
    发明申请
    METHODS AND APPARATUSES FOR IDENTIFYING GESTURES BASED ON ULTRASOUND DATA 审中-公开

    公开(公告)号：US20190196600A1

    公开(公告)日：2019-06-27

    申请号：US16229765

    申请日：2018-12-21

    Applicant: Jonathan M. Rothberg ,  Tyler S. Ralston ,  Nathan Silberman

    Inventor： Jonathan M. Rothberg ,  Tyler S. Ralston ,  Nathan Silberman

    IPC: G06F3/01 ,  G06F3/0346 ,  G06K9/00 ,  G06N20/00

    CPC classification number: G06F3/017 ,  G06F3/0346 ,  G06K9/00335 ,  G06N20/00

    Abstract: Aspects of the technology described herein relate to methods and apparatuses for identifying gestures based on ultrasound data. Performing gesture recognition may include obtaining, with a wearable device, ultrasound data corresponding to an anatomical gesture; and identifying the anatomical gesture based on the obtained ultrasound data. Interfacing with a computing device may include identifying, with a wearable device, an anatomical gesture using ultrasound data obtained by the wearable device; and causing the computing device to perform a specific function based on the anatomical gesture identified by the wearable device. Training a wearable device to perform gesture recognition may include obtaining, with the wearable device, ultrasound data corresponding to an anatomical gesture; obtaining non-ultrasound data corresponding to the anatomical gesture; and training a machine learning model accessed by the wearable device to recognize the anatomical gesture based on correlating the non-ultrasound data and the ultrasound data.

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