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公开(公告)号：US09358551B2
公开(公告)日：2016-06-07
申请号：US14928096
申请日：2015-10-30
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Prasanna Thwar , Vamsee K. Pamula , Arjun Sudarsan , Ramakrishna Sista
CPC classification number: B03C7/026 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0887 , B01L2400/0424 , B01L2400/0427 , B03C1/01 , B03C1/288 , B03C5/00 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N33/54326
Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
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公开(公告)号：US20160108432A1
公开(公告)日：2016-04-21
申请号：US14890451
申请日：2014-05-16
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Srikoundinya Punnamaraju , Melissa A. Sandahl , John J. Harrington , Allen E. Eckhardt , Zhishan Hua , Michael G. Pollack
Abstract: The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator.
Abstract translation: 本发明提供了一种设计用于在液滴上对细胞进行电穿孔的液滴致动器。 本发明还提供了用于在液滴致动器上的液滴中对细胞进行电穿孔的方法和系统。
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公开(公告)号：US20160085240A1
公开(公告)日：2016-03-24
申请号：US14958514
申请日：2015-12-03
Applicant: Advanced Liquid Logic, Inc.
Inventor： Theodore Winger
CPC classification number: G05D7/0629 , B01L3/0268 , B01L3/502707 , B01L3/502784 , B01L7/52 , B01L2200/0668 , B01L2300/0681 , B01L2300/0887 , B01L2300/12 , B01L2300/16 , B01L2300/161 , B01L2300/165 , B01L2400/0427 , B01L2400/043 , Y10T137/0318 , Y10T137/206
Abstract: Droplet actuators that include molecular barrier coatings are provided. The molecular barrier coating may be provided atop the conductive layer of the top substrate, atop the droplet operations electrodes of the bottom substrate, or both. Where the conductive layer of the top substrate and/or the droplet operations electrodes of the bottom substrate are formed of an electrically conductive organic polymer, such as poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS), the molecular barrier coating helps to immobilize the contents of the PEDOT:PSS layer. Further, the molecular barrier coating reduces, preferably entirely eliminates, moisture from seeping into the electrically conducting organic polymer. Methods of conducting droplet operations using the disclosed droplet actuators are also provided.
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124.发明授权公开(公告)号：US09238222B2
公开(公告)日：2016-01-19
申请号：US14549123
申请日：2014-11-20
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Cyril Delattre , Arnaud Rival , Vijay Srinivasan
CPC classification number: B01L3/502784 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/5029 , B01L3/52 , B01L2200/06 , B01L2200/0605 , B01L2300/0645 , B01L2300/0816 , B01L2300/088 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448
Abstract: During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.
Abstract translation: 在液滴致动器中的液滴操作期间，液滴操作间隙中的气泡通常在填充液中形成并且中断液滴操作。 本发明提供了用于在液滴致动器中的液滴上进行液滴操作的方法和系统，包括在液滴操作间隙内对液滴进行多个液滴操作并且/或减少 在多个液滴操作期间液滴操作间隙中的电荷。 所述方法和系统减少或消除了液滴操作间隙的填充流体中的气泡形成，从而允许完成多个液滴操作，而不会由于液滴操作间隙中的填充流体中的气泡形成而中断。
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Patent Agency Ranking
公开(公告)号：US20150330943A1
公开(公告)日：2015-11-19
申请号：US14805532
申请日：2015-07-22
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan
IPC: G01N27/447
CPC classification number: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a method of providing a droplet comprising a single cell is provided, wherein the method includes providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium, dispensing from the droplet to provide a dispensed droplet, determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell, and sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations.
Abstract translation: 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了一种提供包括单个电池的液滴的方法，其中所述方法包括提供液滴，其包含由油介质包围的电润湿液滴致动器上的悬浮液，从液滴分配以提供分配的液滴 确定电润湿液滴致动器上的分配液滴是否包括单个电池，并且基于使用电润湿介导的液滴操作的确定步骤的结果，将分配的液滴分配在电润湿液滴致动器上。
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公开(公告)号：US09081007B2
公开(公告)日：2015-07-14
申请号：US14466193
申请日：2014-08-22
Applicant: Advanced Liquid Logic, Inc.
Inventor： Michael G. Pollack , Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
IPC: G01N15/06 , G01N33/00 , G01N33/48 , G01N33/561 , B01F11/00 , B01F13/00 , B01L3/00 , B03C1/02 , B03C1/30 , B03C5/00 , B01L7/00 , G01N35/10
CPC classification number: G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
Abstract translation: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。
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公开(公告)号：US20150107995A1
公开(公告)日：2015-04-23
申请号：US14589314
申请日：2015-01-05
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Ramakrishna Sista , Vamsee Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar
IPC: B01L3/00 , G01N27/447
CPC classification number: B01L3/502792 , B01L3/502761 , B01L7/52 , B01L2200/0647 , B01L2300/0819 , B01L2300/0851 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/161 , B01L2400/0427 , B01L2400/043
Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
Abstract translation: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。 例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠子的乳液的方法，加载珠粒的方法 液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。
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公开(公告)号：US20150075991A1
公开(公告)日：2015-03-19
申请号：US14549123
申请日：2014-11-20
Applicant: Advanced Liquid Logic, Inc.
Inventor： Delattre Cyril , Rival Arnaud , Srinivasan Vijay
CPC classification number: B01L3/502784 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/5029 , B01L3/52 , B01L2200/06 , B01L2200/0605 , B01L2300/0645 , B01L2300/0816 , B01L2300/088 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448
Abstract: During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.
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公开(公告)号：US20140008222A1
公开(公告)日：2014-01-09
申请号：US14027960
申请日：2013-09-16
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt
IPC: B01L3/02
CPC classification number: G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
Abstract: A method of loading a droplet actuator is provided. In one embodiment, the method may include, providing: a droplet actuator loaded with a filler fluid; a reservoir comprising a droplet fluid; and a fluid path extending from the reservoir into the droplet actuator; and forcing filler fluid from one locus in the droplet actuator to another locus in the droplet actuator; or out of the droplet actuator; thereby causing droplet fluid to flow through the fluid path and into the droplet actuator.
Abstract translation: 提供了一种装载液滴致动器的方法。 在一个实施例中，该方法可以包括：提供：负载有填充流体的液滴致动器; 包括液滴流体的储存器; 以及从储存器延伸到液滴致动器中的流体路径; 并且迫使填充液从液滴致动器中的一个轨迹到液滴致动器中的另一个轨迹; 或离开液滴致动器; 从而使液滴流体流过流体路径并进入液滴致动器。
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