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    Capacitance detection in a droplet actuator
    13.
    发明授权
    Capacitance detection in a droplet actuator 有权
    液滴执行器中的电容检测

    公开(公告)号：US09321049B2

    公开(公告)日：2016-04-26

    申请号：US14338889

    申请日：2014-07-23

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

    IPC: G01R27/26 ,  G01N25/08 ,  B01L3/00 ,  G01N27/22

    CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2400/0427 ,  G01N27/22 ,  G01N27/223 ,  G01N27/227 ,  G01N2203/00 ,  G01V3/02

    Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

    Abstract translation: 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。

    Capacitance Detection in a Droplet Actuator
    14.
    发明申请
    Capacitance Detection in a Droplet Actuator 审中-公开
    滴液致动器中的电容检测

    公开(公告)号：US20160096174A1

    公开(公告)日：2016-04-07

    申请号：US14968094

    申请日：2015-12-14

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

    IPC: B01L3/00 ,  G01N27/22 ,  G01V3/02

    CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2400/0427 ,  G01N27/22 ,  G01N27/223 ,  G01N27/227 ,  G01N2203/00 ,  G01V3/02

    Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

    Abstract translation: 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。

    Droplet Actuator Devices and Methods Employing Magnetic Beads
    17.
    发明申请
    Droplet Actuator Devices and Methods Employing Magnetic Beads 审中-公开
    使用磁珠的液滴致动器装置和方法

    公开(公告)号：US20150314293A1

    公开(公告)日：2015-11-05

    申请号：US14719731

    申请日：2015-05-22

    Applicant: Advanced Liquid Logic, Inc. ,  Duke University

    Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

    IPC: B01L3/00 ,  G01N27/447

    CPC classification number: G01N33/54326 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0427 ,  B01L2400/043 ,  C12M25/01 ,  C12M25/16 ,  G01N27/44717 ,  G01N27/44786 ,  G01N33/54386 ,  G01N35/0098 ,  G01N2035/1046

    Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

    Abstract translation: 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器，其包括：（i）包括布置用于在表面上进行液滴操作的电极的基板; （ii）起始液滴，包括：（1）一个或多个磁响应珠; （2）物质的起始量; 和（3）起始体积; （b）将一个或多个磁响应珠磁性地固定在距离目标液滴分裂区一定距离的位置; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分开所述组合的液滴以产生一组液滴，所述液滴包括：（i）包含基本上所有所述一个或多个磁响应珠粒并且具有减少量 物质相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴。

    DROPLET ACTUATOR AND DROPLET-BASED TECHNIQUES
    19.
    发明申请
    DROPLET ACTUATOR AND DROPLET-BASED TECHNIQUES 审中-公开
    DROPLET执行器和基于DROPLET的技术

    公开(公告)号：US20150253284A1

    公开(公告)日：2015-09-10

    申请号：US14493729

    申请日：2014-09-23

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Arjun Sudarsan ,  Zhishan Hua

    IPC: G01N27/447

    CPC classification number: G01N27/447 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/686 ,  G01N33/54326 ,  G01N2035/00425 ,  G01N2035/1058 ,  G01N2035/1062 ,  C12Q2549/101 ,  C12Q2527/15 ,  C12Q2527/107 ,  C12Q2561/113

    Abstract: The invention is directed to certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuator methods for detection of single nucleotide polymorphisms (SNPs) in a DNA sequence using digital microfluidics, including droplet actuator-based sample preparation and SNP analysis. In another embodiment, the invention provides droplet actuator devices and methods for providing integrated sample preparation and multiplexed detection of an infectious agent, such as HIV. In yet another embodiment, the invention provides droplet actuator devices and techniques for PCR amplification and detection of specific nucleic acid sequences using digital microfluidics, including droplet actuator-based sample preparation and target nucleic acid analysis. In yet another embodiment the invention provides methods for performing hot-start PCR on a droplet actuator. In yet another embodiment, the method of the invention combines PCR amplification with pyrosequencing to investigate specific sequences.

    Abstract translation: 本发明涉及某些液滴致动分子技术。 在一个实施方案中，本发明提供用于使用数字微流体（包括基于液滴致动器的样品制备和SNP分析）检测DNA序列中的单核苷酸多态性（SNP）的液滴致动器方法。 在另一个实施方案中，本发明提供了液滴致动器装置和用于提供综合样品制备和多重检测感染因子如HIV的方法。 在另一个实施方案中，本发明提供了使用数字微流体（包括基于液滴致动器的样品制备和靶核酸分析）的PCR扩增和检测特异性核酸序列的液滴致动器装置和技术。 在另一个实施方案中，本发明提供了在液滴致动器上进行热启动PCR的方法。 在另一个实施方案中，本发明的方法将PCR扩增与焦磷酸测序结合以研究特异性序列。

    BEAD MANIPULATION TECHNIQUES
    20.
    发明申请
    BEAD MANIPULATION TECHNIQUES 有权
    珠子操纵技术

    公开(公告)号：US20150238975A1

    公开(公告)日：2015-08-27

    申请号：US14697058

    申请日：2015-04-27

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： VAMSEE K. PAMULA ,  ARJUN SUDARSAN ,  RAMAKRISHNA SISTA

    IPC: B03C5/00

    CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

    Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

    Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

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