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公开(公告)号：US20250066748A1
公开(公告)日：2025-02-27
申请号：US18723633
申请日：2021-12-31
Applicant: BGI Shenzhen
Inventor： Lele Wang , Tao Zeng , Zhenjun Liu , Junyi Chen , Ziyu Zhao , Denghui Li , Fei Guo , Zhouxiang Ji , Ou Wang , Yuxiang Li , Yuliang Dong , Wenwei Zhang , Xun Xu
IPC: C12N9/14 , C12N15/70 , C12Q1/34 , C12Q1/6869
Abstract: The present invention provides a helicase BCH2X, comprising an amino acid sequence represented by any one of SEQ ID NOs: 1-3. The present invention also provides a complex structure comprising the helicase BCH2X and a binding moiety for binding polynucleotides. The present invention also provides a use of the helicase BCH2X or the complex structure comprising same in the control and characterization of polynucleotides and single-molecule nanopore sequencing.
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公开(公告)号：US10344317B2
公开(公告)日：2019-07-09
申请号：US15518760
申请日：2014-10-13
Applicant: BGI SHENZHEN CO., LIMITED
Inventor： Hongyan Han , Chunyu Geng , Guanying Guo , Wenwei Zhang , Hui Jiang , Yuan Jiang
IPC: C12P19/34 , C12Q1/6806 , C12Q1/6855 , C12Q1/686
Abstract: Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5′-end extension primer, wherein the single-stranded 5′-end extension primer comprises a sequencing platform adaptor sequence of a 5′ end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3′-end adaptor sequence to the 3′ end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.
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13.发明授权公开(公告)号：US10023906B2
公开(公告)日：2018-07-17
申请号：US15510904
申请日：2014-10-14
Applicant: BGI SHENZHEN CO., LIMITED
Inventor： Chunyu Geng , Rongrong Guo , Ruoying Chen , Lingyu He , Wenwei Zhang , Hui Jiang
IPC: C12Q1/68 , C12N9/22 , C12Q1/6853 , C12N15/10 , C40B40/08 , C12N11/06 , C12N9/00 , C12Q1/686 , C40B50/06 , C40B50/14 , C12Q1/6806 , C12N15/66
CPC classification number: C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
Abstract: Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagent kit thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5′ end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.
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公开(公告)号：US09890375B2
公开(公告)日：2018-02-13
申请号：US15510877
申请日：2014-09-12
Applicant: BGI SHENZHEN CO., LIMITED
Inventor： Chunyu Geng , Dennis G. Ballinger , Yanyan Zhang , Shujin Fu , Lingyu He , Wenwei Zhang , Hui Jiang
CPC classification number: C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/06 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
Abstract: Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5′-end nucleotide of the first strand has a phosphate group, and the 3′-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5′-end nucleotide of the second strand does not have a phosphate group, and the 3′-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.
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公开(公告)号：US20170275609A1
公开(公告)日：2017-09-28
申请号：US15510877
申请日：2014-09-12
Applicant: BGI SHENZHEN CO., LIMITED
Inventor： Chunyu Geng , Dennis G. Ballinger , Yanyan Zhang , Shujin Fu , Lingyu He , Wenwei Zhang , Hui Jiang
CPC classification number: C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/06 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
Abstract: Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5′-end nucleotide of the first strand has a phosphate group, and the 3′-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5′-end nucleotide of the second strand does not have a phosphate group, and the 3′-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.
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Patent Agency Ranking
公开(公告)号：US12277468B2
公开(公告)日：2025-04-15
申请号：US18651099
申请日：2024-04-30
Applicant: BGI SHENZHEN
Inventor： Wenwei Zhang , Mei Li , Weimao Wang , Yuxiang Li , Xun Xu
Abstract: Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).
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公开(公告)号：US12168785B2
公开(公告)日：2024-12-17
申请号：US17270489
申请日：2018-09-03
Applicant: BGI SHENZHEN
Inventor： Lili Zhai , Lin Wang , Wenwei Zhang , Yuliang Dong , Yue Zheng , Fen Liu
IPC: C12Q1/68 , C12N9/12 , C12Q1/6806 , C12Q1/6869
Abstract: Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).
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18.发明公开公开(公告)号：US20240279724A1
公开(公告)日：2024-08-22
申请号：US18570440
申请日：2021-06-16
Applicant: BGI SHENZHEN
IPC: C12Q1/6844 , C12Q1/34 , C12Q1/527 , C40B50/06
CPC classification number: C12Q1/6844 , C12Q1/34 , C12Q1/527 , C40B50/06
Abstract: Provided is a method for obtaining a double-stranded sequence by single-stranded rolling circle amplification, comprising: 1) performing rolling circle amplification reaction on single-stranded circular DNA by means of a first primer to obtain an amplified sequence, the first primer being complementary to a partial region of the single-stranded circular DNA, and the single-stranded circular DNA having a break mechanism that can cause the single-stranded circular DNA to ring-open; 2) ring-opening the single-stranded circular DNA by means of the break mechanism to obtain single-stranded linear DNA; and 3) using the single-stranded linear DNA as a second primer and using the amplified sequence obtained in step 1) as a template to perform amplification reaction to obtain an amplified double-stranded sequence.
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公开(公告)号：US11649489B2
公开(公告)日：2023-05-16
申请号：US16757719
申请日：2018-10-12
Applicant: BGI SHENZHEN
Inventor： Erkai Liu , Wenwei Zhang , Ao Chen , Chongjun Xu
IPC: C12Q1/68 , C12Q1/6869 , C12Q1/6876
CPC classification number: C12Q1/6869 , C12Q1/6876
Abstract: Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3′ end, a polymerase, a reagent 1 for excising the blocking group attached to the 3′ end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.
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公开(公告)号：US20210340509A1
公开(公告)日：2021-11-04
申请号：US17358856
申请日：2021-06-25
Applicant: BGI SHENZHEN
Inventor： Huanhuan Liu , Na Guo , Huizhen Li , Zhougang Zhang , Hongyan Han , Miaomiao Guo , Yue Zheng , Yuliang Dong , Wenwei Zhang , Chongjun Xu
IPC: C12N9/12 , C12N15/10 , C12Q1/6869 , C12N15/70 , C12Q1/686 , C12Q1/6806
Abstract: The present disclosure relates to a reverse transcriptase and an application thereof. The reverse transcriptase has mutation sites such as R450H compared with the wild-type M-MLV reverse transcriptase. The reverse transcriptase has increased polymerase activity, improved thermal stability, and reduced RNase H activity.
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