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公开(公告)号：US20170362258A1
公开(公告)日：2017-12-21
申请号：US15697313
申请日：2017-09-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
CPC classification number: C07F7/1804 , C08G77/46 , C08G77/50 , C08L83/12 , C08L83/14 , G01N33/5436
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
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公开(公告)号：US20170151558A1
公开(公告)日：2017-06-01
申请号：US15351354
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia , Billy W. Colston, JR. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
Abstract: System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.
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公开(公告)号：US09649635B2
公开(公告)日：2017-05-16
申请号：US15351331
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Kevin D. Ness , Benjamin J. Hindson , Donald A. Masquelier , Erin R. Chia
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods, apparatus, and kits, for forming and concentrating emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, a droplet well, and a channel network interconnecting the wells. The system also may comprise an instrument configured to operatively receive the device and to create (i) a first pressure differential to produce an emulsion collected in the droplet well and (ii) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well.
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公开(公告)号：US10240187B2
公开(公告)日：2019-03-26
申请号：US15389116
申请日：2016-12-22
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Erin R. Chia
IPC: C12Q1/68 , B01F13/00 , B01F5/06 , G01N1/38 , G01N21/64 , C12Q1/6816 , B01F5/00 , B01F9/00 , B01F11/00 , C12Q1/6806 , B01L3/00 , G01N1/28
Abstract: Method of detecting a signal from droplets for instrument calibration. In the method, a calibration standard for a droplet detection instrument may be received. The calibration standard may have been shipped at least one kilometer when received. The calibration standard may include a mixture of first droplets and second droplets. Each of the first and second droplets may be encapsulated by an immiscible carrier liquid and may have a stabilizing droplet skin that is formed by heating and that includes a skin-forming protein. Each the first droplets may contain a fluorescent dye, and each of the second droplets may contain a fluorescent dye. A fluorescence signal may be detected from a plurality of the first and second droplets with a droplet detection instrument. The fluorescence signal may be stronger for the first droplets than the second droplets.
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Patent Agency Ranking
公开(公告)号：US20180135100A1
公开(公告)日：2018-05-17
申请号：US15818601
申请日：2017-11-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
IPC: C12Q1/6804 , C12Q1/6844 , C12Q1/6851
Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
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公开(公告)号：US09764322B2
公开(公告)日：2017-09-19
申请号：US15351354
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Kevin D. Ness , Benjamin J. Hindson , Donald A. Masquelier , Erin R. Chia
IPC: B01L3/00 , B01F3/00 , B01F3/08 , B01L9/00 , C12Q1/68 , G01N35/08 , B01F13/00 , B01F5/00 , B01F5/02
CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
Abstract: System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.
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公开(公告)号：US20170056886A1
公开(公告)日：2017-03-02
申请号：US15351335
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods, apparatus, and kits, for forming emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, and a droplet well. The device also may include a channel network having a first channel, a second channel, and a third channel that meet one another in a droplet-generation region. The system also may comprise a holder for the device. The system further may comprise an instrument configured to operatively receive an assembly including the device and the holder and to drive sample-containing fluid from the sample well to the droplet-generation region via the first channel, continuous-phase fluid from the continuous-phase well to the droplet-generation region via the second channel, and sample-containing droplets from the droplet-generation region to the droplet well via the third channel.
Abstract translation: 用于形成乳液的系统，包括方法，装置和试剂盒。 示例性系统可以包括包括样品阱的样品，该样品井被配置成接收含有样品的流体，连续相良好地配置成接收连续相流体和液滴阱。 该设备还可以包括具有在液滴生成区域中彼此相遇的第一通道，第二通道和第三通道的通道网络。 该系统还可以包括用于该装置的保持器。 系统还可以包括被配置为可操作地接收包括装置和保持器的组件并且经由第一通道将样品容纳流体从样品阱驱动到液滴产生区域的仪器，来自连续相的连续相流体 通过第二通道对液滴产生区域以及通过第三通道从液滴产生区域到液滴阱的样品含有液滴。
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公开(公告)号：US09527049B2
公开(公告)日：2016-12-27
申请号：US13921455
申请日：2013-06-19
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Erin R. Chia
IPC: B01F5/00 , B01F9/00 , B01F11/00 , C12Q1/68 , G01N1/38 , B01L3/00 , B01F5/06 , B01F13/00 , G01N1/28
CPC classification number: C12Q1/6816 , B01F5/0085 , B01F5/0647 , B01F9/00 , B01F11/00 , B01F13/0062 , B01F13/0072 , B01F2215/0037 , B01L3/502784 , B01L3/52 , B01L2200/148 , B01L2200/185 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , C12Q1/6806 , G01N1/38 , G01N21/6428 , G01N21/645 , G01N2001/2893 , G01N2021/6439 , G01N2201/127 , C12Q2563/159
Abstract: Provided herein, are droplet mixture compositions and systems and methods for forming mixtures of droplets. The system may comprise two or more droplet generation units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The combined droplet populations can be mixed, heated, and collected for multiple uses, such as for use as calibration standards for instrument testing and analysis.
Abstract translation: 本文提供了液滴混合物组合物和用于形成液滴混合物的系统和方法。 该系统可以包括两个或更多个液滴产生单元。 每个单元可以包括至少一个第一输入井，第二输入井和通过形成液滴发生器的通道连接到第一和第二输入井的输出井。 组合的液滴种群可以混合，加热和收集用于多种用途，例如用作仪器测试和分析的校准标准。
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公开(公告)号：US09328376B2
公开(公告)日：2016-05-03
申请号：US14018205
申请日：2013-09-04
Applicant: BIO-RAD LABORATORIES, INC.
Inventor： Amy L. Hiddessen , Erin R. Chia
CPC classification number: C12Q1/6806 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2300/0816 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2400/0487
Abstract: This disclosure provides systems, devices, and methods for sample preparation and/or analysis comprising a droplet generator having a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel may meet at an intersection that receives a sample from the sample reservoir and a carrier fluid from the carrier fluid reservoir and generates one or more droplets that flow along a droplet channel to a droplet reservoir. An energy application member in thermal communication with the intersection, at least a portion of the droplet channel, the sample reservoir and/or the carrier fluid reservoir may provide energy to an individual droplet of the one or more droplets, such as at the intersection and/or as the droplet moves along the droplet channel to the droplet reservoir.
Abstract translation: 本公开提供了用于样品制备和/或分析的系统，装置和方法，其包括具有与载体流体储存器流体连通的第一通道和与样品储存器流体连通的第二通道的液滴发生器。 第一通道和第二通道可以在从样品储存器接收样品和来自载体流体储存器的载体流体的交叉点处相遇，并且产生沿着液滴通道流动到液滴储存器的一个或多个液滴。 与交叉点热连通的能量施加构件，液滴通道，样品储存器和/或载体流体储存器的至少一部分可以向一个或多个液滴的单个液滴（例如在交叉点处）提供能量，并且 /或当液滴沿着液滴通道移动到液滴储存器时。
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