公开(公告)号：US20190242795A1

公开(公告)日：2019-08-08

申请号：US16236116

申请日：2018-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, JR. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: G01N1/30 ,  B01J19/00 ,  C12Q1/6876 ,  G01N1/44

Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

公开(公告)号：US10258988B2

公开(公告)日：2019-04-16

申请号：US15365883

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Benjamin J. Hindson ,  Billy W. Colston, Jr. ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  C12Q1/686 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B29L31/00

Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.

公开(公告)号：US10167509B2

公开(公告)日：2019-01-01

申请号：US14848311

申请日：2015-09-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, Jr.

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6883 ,  C12Q1/683 ,  C12Q1/6858

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

公开(公告)号：US10166522B2

公开(公告)日：2019-01-01

申请号：US14938470

申请日：2015-11-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, Jr. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: C12Q1/68 ,  B01J19/00 ,  G01N21/75 ,  C12Q1/6844 ,  G01N33/50 ,  B01F13/00 ,  B01L3/00 ,  G01N35/08 ,  B01L7/00 ,  G01N1/30 ,  G01N1/44 ,  C07H21/02 ,  C40B50/08 ,  C40B60/14

Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

公开(公告)号：US09764322B2

公开(公告)日：2017-09-19

申请号：US15351354

申请日：2016-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Kevin D. Ness ,  Benjamin J. Hindson ,  Donald A. Masquelier ,  Erin R. Chia

IPC: B01L3/00 ,  B01F3/00 ,  B01F3/08 ,  B01L9/00 ,  C12Q1/68 ,  G01N35/08 ,  B01F13/00 ,  B01F5/00 ,  B01F5/02

CPC classification number: B01L3/50273 ,  B01F3/0807 ,  B01F5/0085 ,  B01F5/0256 ,  B01F13/0062 ,  B01F13/0071 ,  B01F13/1022 ,  B01L3/502 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  C12Q1/686 ,  G01N35/08

Abstract: System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.

公开(公告)号：US20170144160A1

公开(公告)日：2017-05-25

申请号：US15365883

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Benjamin J. Hindson ,  Billy W. Colston, JR. ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01L3/02 ,  B01F15/00 ,  B01F3/08 ,  B01F13/00

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F15/00922 ,  B01F2003/0834 ,  B01F2003/0842 ,  B01F2215/0037 ,  B01L3/0241 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B29C45/0053 ,  B29C45/006 ,  B29C2045/0079 ,  B29L2031/752 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G06F19/00 ,  Y02A90/26

Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.

公开(公告)号：US20170056886A1

公开(公告)日：2017-03-02

申请号：US15351335

申请日：2016-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Donald A. Masquelier ,  Kevin D. Ness ,  Benjamin J. Hindson ,  Anthony J. Makarewicz, JR. ,  Erin R. Chia

IPC: B01L3/00 ,  B01F13/00 ,  C12Q1/68 ,  B01F3/08

CPC classification number: B01L9/527 ,  B01F3/0807 ,  B01F13/0062 ,  B01F13/0071 ,  B01L3/0241 ,  B01L3/502 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/52 ,  B01L7/525 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/1415

Abstract: System, including methods, apparatus, and kits, for forming emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, and a droplet well. The device also may include a channel network having a first channel, a second channel, and a third channel that meet one another in a droplet-generation region. The system also may comprise a holder for the device. The system further may comprise an instrument configured to operatively receive an assembly including the device and the holder and to drive sample-containing fluid from the sample well to the droplet-generation region via the first channel, continuous-phase fluid from the continuous-phase well to the droplet-generation region via the second channel, and sample-containing droplets from the droplet-generation region to the droplet well via the third channel.

Abstract translation: 用于形成乳液的系统，包括方法，装置和试剂盒。 示例性系统可以包括包括样品阱的样品，该样品井被配置成接收含有样品的流体，连续相良好地配置成接收连续相流体和液滴阱。 该设备还可以包括具有在液滴生成区域中彼此相遇的第一通道，第二通道和第三通道的通道网络。 该系统还可以包括用于该装置的保持器。 系统还可以包括被配置为可操作地接收包括装置和保持器的组件并且经由第一通道将样品容纳流体从样品阱驱动到液滴产生区域的仪器，来自连续相的连续相流体 通过第二通道对液滴产生区域以及通过第三通道从液滴产生区域到液滴阱的样品含有液滴。

公开(公告)号：US20130302792A1

公开(公告)日：2013-11-14

申请号：US13945661

申请日：2013-07-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, JR. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  C12Q1/6848 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6441 ,  G01N2201/1242 ,  C12Q2545/101 ,  C12Q2563/107 ,  C12Q2563/159

Abstract: System, including methods and apparatus, for performing droplet-based assays that are controlled and/or calibrated using signals detected from droplets.

Abstract translation: 用于执行使用从液滴检测的信号来控制和/或校准的基于液滴的测定的系统，包括方法和装置。

公开(公告)号：US20250091048A1

公开(公告)日：2025-03-20

申请号：US18888785

申请日：2024-09-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Serge Saxonov ,  Phillip Belgrader ,  Kevin D. Ness ,  Michael Y. Lucero ,  Billy W. Colston, JR. ,  Shawn Paul Hodges ,  Nicholas J. Heredia ,  Jeffrey Clark Mellen ,  Camille Bodley Troup ,  Paul Wyatt

IPC: B01L3/00 ,  B01F23/41 ,  B01F25/00 ,  B01F25/23 ,  B01F33/3011 ,  B01F33/302 ,  B01F33/81 ,  B01L9/00 ,  C12Q1/686 ,  G01N35/08

Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

公开(公告)号：US12168231B2

公开(公告)日：2024-12-17

申请号：US17486804

申请日：2021-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Billy W. Colston, Jr. ,  Benjamin J. Hindson ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/10 ,  B01L3/02 ,  B01L7/00 ,  B29C45/00 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/64 ,  B01F101/23 ,  B29L31/00

Abstract: Method of analysis. In the method, a microfluidic device defining a flow path extending from an inlet to an outlet may be selected. A sample-containing fluid may be introduced into the flow path via the inlet. Volumes of the sample-containing fluid may be isolated from one another on the flow path. A two-dimensional monolayer of the volumes may be imaged. The two-dimensional monolayer may be formed along the flow path between the inlet and the outlet.