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    DNA sequencing by mass spectrometry via exonuclease degradation
    12.
    发明授权
    DNA sequencing by mass spectrometry via exonuclease degradation 有权
    通过外切核酸酶降解的质谱进行DNA测序

    公开(公告)号：US06140053A

    公开(公告)日：2000-10-31

    申请号：US160671

    申请日：1998-09-25

    Applicant: Hubert Koster

    Inventor： Hubert Koster

    IPC: C07H21/00 ,  C12Q1/68 ,  G01N35/10

    CPC classification number: C07H21/00 ,  C12Q1/6858 ,  C12Q1/6872 ,  G01N35/1067

    Abstract: The invention provides fast and highly accurate mass spectrometer based processes for directly sequencing a target nucleic acid (or fragments generated from the target nucleic acid), which by means of protection, specificity of enzymatic activity, or immobilization, are unilaterally degraded in a stepwise manner via exonuclease digestion and the nucleotides, derivatives or truncated sequences detected by mass spectrometry.

    Abstract translation: 本发明提供了用于直接测序靶核酸（或由靶核酸产生的片段）的快速且高度精确的基于质谱仪的方法，其通过保护酶活性或固定化的特异性以逐步方式单方面降解 通过核酸外切酶消化和通过质谱法检测的核苷酸，衍生物或截短序列。

    Process for the preparation of oligonucleotides in solution
    15.
    发明授权
    Process for the preparation of oligonucleotides in solution 失效
    在溶液中制备寡核苷酸的方法

    公开(公告)号：US5198540A

    公开(公告)日：1993-03-30

    申请号：US368965

    申请日：1989-06-19

    Applicant: Hubert Koster

    Inventor： Hubert Koster

    IPC: C07H21/00

    CPC classification number: C07H21/00

    Abstract: Process for the preparation of oligonucleotides by successive linking of individual or several nucleoside and/or nucleotide units in a homogeneous phase system using a bi-, tri- or tetra-functional carrier molecule of the general formula Sp(X).sub.n, wherein X is a reactive group which is compatible from the point of view of nucleotide chemistry and n is an integer from 2 to 4, the sequence SP(X-N.sup.1 -N.sup.2 . . . N.sup.m).sub.n being built up stepwise on the carrier molecule and the oligonucleotide of the formula N.sup.1 -N.sup.2 . . . N.sup.m subsequently being released.

    Abstract translation: PCT No.PCT / EP83 / 00281 Sec。 371日期1984年6月25日第 102（e）日期1984年6月25日PCT提交1983年10月27日PCT。通过使用双相，三联体或三元共聚物在均相系统中连续连接单个或多个核苷和/或核苷酸单元来制备寡核苷酸的方法 通式为Sp（X）n的四官能载体分子，其中X是从核苷酸化学的角度考虑相容的反应性基团，n为2〜4的整数，序列SP（X-N1- N2 ... Nm）n在载体分子和式N1-N2的寡核苷酸上逐步构建。 。 。 随后被释放。

    Process for the preparation of oligonucleotides
    16.
    再颁专利
    Process for the preparation of oligonucleotides 失效
    制备寡核苷酸的方法

    公开(公告)号：USRE34069E

    公开(公告)日：1992-09-15

    申请号：US481572

    申请日：1990-02-16

    Applicant: Hubert Koster ,  Nanda D. Sinha

    Inventor： Hubert Koster ,  Nanda D. Sinha

    IPC: C07H21/00

    CPC classification number: C07H21/00

    Abstract: The invention relates to a process for the preparation of oligonucleotides by the following steps: reaction of a nucleoside with a phosphine derivative, reaction of the nucleotide derivative thus obtained with a nucleoside bonded to a polymeric carrier, oxidation of the carrier-bound nucleoside-nucleotide thus obtained with formation of phosphotriester groups, blocking of free primary 5'--OH groups, elimination of a protective group from the terminal 5'--OH group, where appropriate single or multiple repetition of the abovementioned steps to introduce further nucleoside phosphate or oligonucleoside phosphate units, and cleavage of the nucleoside-carrier bond and, where appropriate, elimination of all protective groups present in the oligonucleoside phosphates. The phosphine derivative used is a compound of the general formula III ##STR1## in which X and L can react with OH groups of the sugar units in the oligonucleotides, and R.sup.3 is a protective group which can be liberated by .beta.-elimination.

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