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公开(公告)号：US07192559B2
公开(公告)日：2007-03-20
申请号：US09922224
申请日：2001-08-02
CPC分类号： B01D61/18 , B01L3/5025 , B01L3/502707 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L2200/027 , B01L2300/0861 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/043 , B01L2400/0457 , Y10T137/2496 , Y10T137/2499 , Y10T137/2501 , Y10T137/2514 , Y10T137/2516 , Y10T436/11 , Y10T436/2575
摘要： Methods and devices for delivering fluids into microfluidic device body structures are described. The methods and devices include the use of fluid manifolds which are integrated or interchangeable with device body structures. Methods of fabricating manifolds are also provided.
摘要翻译： 描述了将流体输送到微流体装置体结构中的方法和装置。 所述方法和装置包括使用与装置主体结构集成或可互换的流体歧管。 还提供了制造歧管的方法。
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12.发明授权Microfluidic devices, systems and methods for performing integrated reactions and separations 失效用于执行集成反应和分离的微流体装置，系统和方法公开(公告)号：US06551836B1
公开(公告)日：2003-04-22
申请号：US09442073
申请日：1999-11-15
IPC分类号： G01N27447
CPC分类号： B01L3/50273 , B01F13/0059 , B01L3/502753 , B01L3/502784 , B01L2200/0605 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , G01N27/44743 , G01N27/44791 , G01N2001/4038 , Y10T436/117497 , Y10T436/25375 , Y10T436/2575
摘要： Microfluidic devices for performing integrated reaction and separation operations. The devices have a planar substrate having a first surface with an integrated channel network disposed therein. The reaction region in the integrated microscale channel network has a mixture of at least first and second reactants located therein, wherein the mixture interacts to produce one or more products. The reaction region is configured to maintain contact between the first and second reactants contained within it. The device also includes a separation region in the integrated channel network, where the separation region is configured to separate the first reactant from the product, when the first reactant and product are flowing through the separation region.
摘要翻译： 用于进行集成反应和分离操作的微流体装置。 这些装置具有平面基板，其具有设置在其中的集成通道网络的第一表面。 集成微量通道网络中的反应区域具有位于其中的至少第一和第二反应物的混合物，其中混合物相互作用以产生一种或多种产物。 反应区域被配置为保持包含在其中的第一和第二反应物之间的接触。 该装置还包括在集成通道网络中的分离区域，其中当第一反应物和产物流过分离区域时，分离区域被配置为将第一反应物与产物分离。
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公开(公告)号：US07344865B2
公开(公告)日：2008-03-18
申请号：US11460550
申请日：2006-08-11
申请人： J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
发明人： J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
CPC分类号： C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
摘要翻译： 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止，用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序，通过掺入荧光标记的核苷酸进行检测，任选地随后进行光漂白，并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件，包括粒子阵列，用于测序方法。
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公开(公告)号：US06613580B1
公开(公告)日：2003-09-02
申请号：US09609030
申请日：2000-06-30
IPC分类号： C01N110
CPC分类号： G01N27/44791 , B01J19/0093 , B01J2219/00317 , B01J2219/00702 , B01J2219/00889 , B01J2219/00891 , B01J2219/00952 , B01J2219/00995 , B01L3/5027 , C12Q1/00 , C12Q1/686 , C40B60/14 , G01N27/44726 , G01N2500/00 , Y10T436/25 , Y10T436/25625 , Y10T436/2575 , C12Q2565/629
摘要： Methods, systems, kits, and apparatus for calculating kinetic and concentration information in microscale systems are provided. Dwell times for access by a microfluidic system are varied and the resulting modulation of signal profile information used to provide a first order estimation of an activity of a potential activity modulator accessed by the system.
摘要翻译： 提供了用于计算微量系统中的动力学和浓度信息的方法，系统，试剂盒和装置。 由微流体系统访问的停留时间是变化的，并且所得到的信号分布信息的调制用于提供系统访问的潜在活动调制器的活动的一阶估计。
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公开(公告)号：US06613513B1
公开(公告)日：2003-09-02
申请号：US09510205
申请日：2000-02-22
申请人： J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
发明人： J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
IPC分类号： C12Q168
CPC分类号： C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
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公开(公告)号：US09101928B2
公开(公告)日：2015-08-11
申请号：US13015242
申请日：2011-01-27
申请人： Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
发明人： Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
IPC分类号： G01N35/00 , B01L3/00 , C07H21/02 , C07H21/04 , C12Q1/68 , B01F13/00 , C40B60/14 , G01N27/447
CPC分类号： C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要： An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
摘要翻译： 在微流体装置中使用可移动颗粒组的阵列，用于在微流体装置中执行化学反应。 微流体装置包括主通道和相交的侧通道，主通道和侧通道形成多个交叉点。 颗粒组的阵列设置在主通道中，并且侧通道与试剂偶联。 当颗粒组通过主通道和侧通道的交叉点传输时，试剂通过侧通道流动与每个阵列构件（或选定的阵列构件）接触，从而在微流体系统中提供多个化学反应。
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公开(公告)号：US20110118139A1
公开(公告)日：2011-05-19
申请号：US13015242
申请日：2011-01-27
申请人： Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
发明人： Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
CPC分类号： C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要： An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
摘要翻译： 在微流体装置中使用可移动颗粒组的阵列，用于在微流体装置中进行化学反应。 微流体装置包括主通道和相交的侧通道，主通道和侧通道形成多个交叉点。 颗粒组的阵列设置在主通道中，并且侧通道与试剂偶联。 当颗粒组通过主通道和侧通道的交叉点传输时，试剂通过侧通道流动与每个阵列构件（或选定的阵列构件）接触，从而在微流体系统中提供多个化学反应。
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公开(公告)号：US20090137413A1
公开(公告)日：2009-05-28
申请号：US11928808
申请日：2007-10-30
申请人： Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
发明人： Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
IPC分类号： C40B30/04
CPC分类号： C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要： Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.
摘要翻译： 可流动或固定的颗粒组的数组用于微流体系统中，用于进行测定和改变流体动力学流动。 还提供了利用微流体系统内的可流动或固定的颗粒组的测定法，以及包括阵列和阵列构件的试剂盒，装置和集成系统。
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19.发明授权Microfluidic devices, systems and methods for performing integrated reactions and separations 失效用于执行集成反应和分离的微流体装置，系统和方法公开(公告)号：US06274089B1
公开(公告)日：2001-08-14
申请号：US09093489
申请日：1998-06-08
IPC分类号： G01N2726
CPC分类号： B01L3/50273 , B01F13/0059 , B01L3/502753 , B01L2200/0605 , B01L2300/0816 , B01L2300/0867 , B01L2400/0421
摘要： Microfluidic devices for performing integrated reaction and separation operations. The devices include a planar substrate having a first surface with an integrated channel network disposed therein. The reaction region in the integrated microscale channel network has a mixture of at least first and second reactants located therein, wherein the mixture interacts to produce one or more products. The reaction region is configured to maintain contact between the first and second reactants contained within it. The device also includes a separation region in the integrated channel network, where the separation region is configured to separate the first reactant from the product, when the first reactant and product are flowing through the separation region. The conductivity of a fluid in the reaction region is higher than the conductivity of a fluid in the separation region.
摘要翻译： 用于进行集成反应和分离操作的微流体装置。 这些装置包括具有设置在其中的集成通道网络的第一表面的平面基板。 集成微量通道网络中的反应区域具有位于其中的至少第一和第二反应物的混合物，其中混合物相互作用以产生一种或多种产物。 反应区域被配置为保持包含在其中的第一和第二反应物之间的接触。 该装置还包括在集成通道网络中的分离区域，其中当第一反应物和产物流过分离区域时，分离区域被配置为将第一反应物与产物分离。 反应区域中的流体的电导率高于分离区域中的流体的电导率。
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公开(公告)号：US06881312B2
公开(公告)日：2005-04-19
申请号：US10319045
申请日：2002-12-13
申请人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
发明人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
IPC分类号： B01L3/00 , B01L7/00 , B01L9/00 , G01N21/05 , G01N21/64 , G01N27/447 , G01N35/00 , G01N37/00 , G01N27/26
CPC分类号： G01N21/6452 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00628 , B01J2219/0063 , B01J2219/00659 , B01J2219/00707 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/54 , B01L9/527 , B01L2200/023 , B01L2200/027 , B01L2200/028 , B01L2200/147 , B01L2300/0654 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N27/44721 , G01N27/44782 , G01N35/0099 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421
摘要： Analytical systems and methods that use a modular interface structure for providing an interface between a sample substrate and an analytical unit, where the analytical unit typically has a particular interface arrangement for implementing various analytical and control functions. Using a number of variants for each module of the modular interface structure advantageously provides cost effective and efficient ways to perform numerous tests using a particular substrate or class of substrates with a particular analytical and control systems interface arrangement. Improved optical illumination and detection system for simultaneously analyzing reactions or conditions in multiple parallel microchannels are also provided. Increased throughput and improved emissions detection is provided by the present invention by simultaneously illuminating multiple parallel microchannels at a non-normal incidence using an excitation beam including multiple excitation frequencies, and simultaneously detecting emissions from the substances in the microchannels in a direction normal to the substrate using a detection module with multiple detectors.
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