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71 条记录 (耗时 0.031 秒)

    Apparatus for circulating carrier fluid
    13.
    发明授权
    Apparatus for circulating carrier fluid 失效
    载体流体循环装置

    公开(公告)号：US07329535B2

    公开(公告)日：2008-02-12

    申请号：US10292012

    申请日：2002-11-11

    申请人： Kwang-wook Oh ,  Geun-bae Lim ,  Young-sun Lee ,  Yoon-kyoung Cho

    发明人： Kwang-wook Oh ,  Geun-bae Lim ,  Young-sun Lee ,  Yoon-kyoung Cho

    IPC分类号： C12M1/34 ,  C12M3/00 ,  F16K13/00 ,  B01L11/00 ,  B32B5/02

    CPC分类号： B01L7/525 ,  B01L3/502738 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/0694 ,  Y10T137/4643 ,  Y10T137/4651

    摘要： Provided are an apparatus for circulating a carrier fluid having two or more chambers or sections, an apparatus for amplifying a nucleic acid using the same, and a chip containing the same. The apparatus for circulating a carrier fluid includes two or more chambers maintained at different temperatures, each chamber having an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber (inlet pneumatic air pressure port), and an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber (outlet pneumatic air pressure port), wherein the chambers are sequentially connected such that the outlet valve of one chamber is connected to the inlet valve of an adjacent chamber in a direction the fluid flows.

    摘要翻译： 提供一种用于循环具有两个或更多个室或部分的载体流体的装置，用于使用其的核酸的放大装置和含有该核酸的芯片的装置。 用于循环载体流体的装置包括保持在不同温度的两个或更多个室，每个室具有入口阀，该入口阀包含用于控制载体流体流入室（进气气压端口）的气动空气压力端口，以及出口 阀包含用于控制从腔室（出口气动空气压力端口）流出的载气的气动空气压力，其中腔室顺序地连接，使得一个室的出口阀连接到相邻室的入口阀 方向流体流动。

    Method and apparatus for determining zeta potential using alternating current electric field and T channel
    14.
    发明授权
    Method and apparatus for determining zeta potential using alternating current electric field and T channel 失效
    使用交流电场和T通道确定ζ电位的方法和装置

    公开(公告)号：US07338808B2

    公开(公告)日：2008-03-04

    申请号：US10698507

    申请日：2003-10-31

    申请人： Yoon-kyoung Cho ,  Sang-min Shin ,  In-seok Kang ,  Geun-bae Lim

    发明人： Yoon-kyoung Cho ,  Sang-min Shin ,  In-seok Kang ,  Geun-bae Lim

    IPC分类号： G01N25/18 ,  G01N27/00 ,  B01L3/02 ,  B32B5/02 ,  B32B27/04

    CPC分类号： B01F13/0076 ,  B01L3/50273 ,  B01L3/502769 ,  B01L2400/0418 ,  G01N27/44752 ,  Y10T436/11

    摘要： Provided is a method for determining a zeta potential generated between a solid wall and a solution. The method includes (a) injecting an electrolyte solution into a first inlet of a T channel, which is provided with first and second inlet electrodes and a grounded outlet electrode, and a mixed solution of the electrolyte solution and a fluorescent dye into a second channel of the T channel and maintaining a steady-state of the two solutions; (b) applying a direct current electric field from the first and second electrodes to the outlet electrode to form an interface between the electrolyte solution and the mixed solution; (c) applying an alternating current electric field from one of the two inlet electrodes to the outlet electrode to oscillate the interface; and (d) measuring an amplitude of oscillation of the interface and determining the zeta potential from the standard relationship between the zeta potential and the amplitude.

    摘要翻译： 提供了一种确定固体壁和溶液之间产生的ζ电位的方法。 该方法包括：（a）将电解质溶液注入T沟道的第一入口，T通道的第一入口设置有第一入口电极和第二入口电极和接地的出口电极，以及电解质溶液和荧光染料的混合溶液到第二通道 的T通道，并保持两种解决方案的稳定状态; （b）将直流电场从第一和第二电极施加到出口电极以形成电解质溶液和混合溶液之间的界面; （c）将两个入口电极之一的交流电场施加到出口电极以使界面振荡; 和（d）测量界面的振荡幅度，并根据ζ电位和幅度之间的标准关系确定ζ电位。

    Method of concentrating and disrupting cells or viruses
    19.
    发明申请
    Method of concentrating and disrupting cells or viruses 审中-公开
    浓缩和破坏细胞或病毒的方法

    公开(公告)号：US20080090275A1

    公开(公告)日：2008-04-17

    申请号：US11783351

    申请日：2007-04-09

    申请人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Jong-myeon Park ,  Young-sun Lee ,  Hyo-yeon Lee

    发明人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Jong-myeon Park ,  Young-sun Lee ,  Hyo-yeon Lee

    IPC分类号： C12N13/00

    CPC分类号： C12N1/066 ,  C12M47/06 ,  C12N13/00 ,  G01N33/54326

    摘要： Provided is a method for concentrating and disrupting cells or viruses, the method employs magnetic particles which are capable of binding the cells or viruses to capture the cells or viruses and a laser to disrupt the cells or viruses. Since the same particles are used in target cell separation and cell lysis, there is no need to add additional particles in a laser lysis process, and thus integration of a target cell separation, concentration, purification, and nucleic acid extraction process is easy.

    摘要翻译： 提供了一种用于浓缩和破坏细胞或病毒的方法，该方法使用能够结合细胞或病毒以捕获细胞或病毒的磁性颗粒和激光以破坏细胞或病毒。 由于在靶细胞分离和细胞裂解中使用相同的颗粒，因此不需要在激光裂解过程中添加额外的颗粒，因此靶细胞分离，浓缩，纯化和核酸提取过程的整合是容易的。