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公开(公告)号：US07579172B2
公开(公告)日：2009-08-25
申请号：US11071383
申请日：2005-03-03
Applicant: Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
Inventor： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
CPC classification number: G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
Abstract: An apparatus for amplifying nucleic acids includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate, wherein the reactant aqueous solution contacts the fluid to create a plurality of droplets surrounded by the fluid in the reaction vessel and the nucleic acids are amplified in the reactant aqueous solution droplets.
Abstract translation: 用于放大核酸的装置包括：基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许引入含有反应物的反应物水溶液， 核酸扩增到反应容器中，形成在基底内的第二入口通道，连接到反应容器的末端，并允许引入与反应物水溶液相分离并且不参与扩增反应的流体 反应容器和加热单元，其以与基底热接触并加热基底的方式安装在基底上，其中反应物水溶液接触流体以产生由反应容器中的流体包围的多个液滴， 核酸在反应物水溶液液滴中被扩增。
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22.发明申请METHODS OF MAKING A MOLECULAR DETECTION CHIP HAVING A METAL OXIDE SILICON FIELD EFFECT TRANSISTOR ON SIDEWALLS OF A MICRO-FLUID CHANNEL 有权在微流体通道上形成金属氧化物半导体场效应晶体管的分子检测芯片的方法公开(公告)号：US20070218610A1
公开(公告)日：2007-09-20
申请号：US11755952
申请日：2007-05-31
Applicant: Geun-Bae Lim , Chin-Sung Park , Yoon-Kyoung Cho , Sun-Hee Kim
Inventor： Geun-Bae Lim , Chin-Sung Park , Yoon-Kyoung Cho , Sun-Hee Kim
IPC: H01L21/22
CPC classification number: G01N33/5438 , B01J2219/00497 , B01J2219/00511 , B01J2219/00585 , B01J2219/00596 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B01J2219/00743 , B01L3/5027 , B82Y15/00 , B82Y30/00 , C12Q1/6837 , G01N27/4145 , G01N33/54373 , C12Q2565/607
Abstract: A molecular detection chip including a metal oxide silicon-field effect transistor (MOSFET) on sidewalls of a micro-fluid channel and a molecular detection device including the molecular detection chip are provided. A molecular detection method, particularly, qualification methods for the immobilization of molecular probes and the binding of a target sample to the molecular probes, using the molecular detection device, and a nucleic acid mutation assay device and method are also provided. The formation of the MOSFET on the sidewalls of the micro-fluid channel makes easier to highly integrate a molecular detection chip. In addition, immobilization of probes directly on the surface of a gate electrode ensures the molecular detection chip to check for the immobilization of probes and coupling of a target molecule to the probes in situ.
Abstract translation: 提供了包括在微流体通道的侧壁上的金属氧化物硅场效应晶体管（MOSFET）和包括分子检测芯片的分子检测装置的分子检测芯片。 还提供了使用分子检测装置的分子检测方法，特别是用于固定分子探针的鉴定方法和靶样品与分子探针的结合，以及核酸突变测定装置和方法。 在微流体通道的侧壁上形成MOSFET更容易高度集成分子检测芯片。 此外，探针直接固定在栅电极的表面上确保了分子检测芯片，以检查探针的固定和目标分子与探针原位偶联。
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公开(公告)号：US07135294B2
公开(公告)日：2006-11-14
申请号：US10658169
申请日：2003-09-09
Applicant: Jae-hoon Lee , Jae-chang Lee , Dae-sung Yoon , Geun-bae Lim
Inventor： Jae-hoon Lee , Jae-chang Lee , Dae-sung Yoon , Geun-bae Lim
CPC classification number: C12Q1/6825 , C12Q1/686 , G01N27/221 , C12Q2565/607 , C12Q2561/113 , C12Q2531/113
Abstract: A method for detecting a polymerase chain reaction (PCR) product is provided. The method includes (a) providing at least a pair of electrodes in a PCR solution-containing vessel; (b) performing PCR; (c) producing an electric field between the electrodes; and (d) measuring a change in a dielectric property in the PCR solution. Therefore, a PCR product can be detected in real time.
Abstract translation: 提供了一种检测聚合酶链反应（PCR）产物的方法。 该方法包括（a）在含有PCR溶液的容器中提供至少一对电极; （b）进行PCR; （c）在电极之间产生电场; 和（d）测量PCR溶液中介电性质的变化。 因此，可以实时检测PCR产物。
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24.发明授权Method and sensor for detecting the binding of biomolecules by shear stress measurement 有权用于通过剪切应力测量检测生物分子的结合的方法和传感器公开(公告)号：US07112452B2
公开(公告)日：2006-09-26
申请号：US10278691
申请日：2002-10-23
Applicant: Yoon-kyoung Cho , Sun-hee Kim , Kwang-wook Oh , Geun-bae Lim , Dae-sung Yoon
Inventor： Yoon-kyoung Cho , Sun-hee Kim , Kwang-wook Oh , Geun-bae Lim , Dae-sung Yoon
IPC: G01N33/543
CPC classification number: C12Q1/6825 , Y10S436/806 , C12Q2565/107 , C12Q2565/607
Abstract: A method and sensor are provided for detecting the binding of a probe and a target biomolecule by measuring a difference in the shear stress on the surface of the sensor before and after hybridization of the target molecule to the probe, such as nucleic acids or proteins. The shear stress may be measured sensitively and conveniently as an electrical signal without additional fluorescent labeling and without use of expensive additional devices.
Abstract translation: 提供了一种方法和传感器，用于通过测量靶分子与探针如核酸或蛋白质杂交前后的传感器表面上的剪切应力差异来检测探针和靶生物分子的结合。 剪切应力可以作为电信号被灵敏地和方便地测量，而不需要额外的荧光标记，而不使用昂贵的附加装置。
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25.发明申请Method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or amino group 审中-公开使用涂覆有羧基或氨基的固相材料来扩增核酸的方法公开(公告)号：US20050186602A1
公开(公告)日：2005-08-25
申请号：US11042376
申请日：2005-01-25
Applicant: Joon-ho Kim , Yoon-kyoung Cho , Jung-joo Hwang , Geun-bae Lim , Jeong-gun Lee
Inventor： Joon-ho Kim , Yoon-kyoung Cho , Jung-joo Hwang , Geun-bae Lim , Jeong-gun Lee
CPC classification number: C12N15/1006
Abstract: A method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or an amino group is provided. The method includes contacting a mixture of a nucleic acid containing sample and a salt solution with the solid phase material coated with a carboxyl group or an amino group to form a nucleic acid-solid phase material complex, washing the nucleic acid-solid phase material complex with a wash buffer, and adding a reaction solution for amplifying a nucleic acid to the nucleic acid-solid phase material complex to perform an amplification reaction.
Abstract translation: 提供了使用涂覆有羧基或氨基的固相材料来扩增核酸的方法。 该方法包括将含有核酸的样品和盐溶液的混合物与涂覆有羧基或氨基的固相材料接触以形成核 - 固相物质络合物，洗涤核酸 - 固相物质络合物 用洗涤缓冲液加入用于将核酸扩增至核酸 - 固相物质络合物的反应溶液进行扩增反应。
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Patent Agency Ranking
公开(公告)号：US20050136463A1
公开(公告)日：2005-06-23
申请号：US11016447
申请日：2004-12-17
Applicant: Joon-ho Kim , Yoon-kyoung Cho , Geun-bae Lim , Soo-hwan Jeong
Inventor： Joon-ho Kim , Yoon-kyoung Cho , Geun-bae Lim , Soo-hwan Jeong
CPC classification number: C12Q1/6806 , C12Q2527/137 , C12Q2523/308
Abstract: A method for isolating a nucleic acid is provided. The method includes mixing a nucleic acid-containing sample, a salt solution, and alumina; isolating the alumina having the nucleic acid attached thereto from the liquid; washing the alumina having the nucleic acid attached thereto; and eluting the nucleic acid from the alumina.
Abstract translation: 提供了分离核酸的方法。 该方法包括混合含核酸的样品，盐溶液和氧化铝; 从液体中分离具有与其连接的核酸的氧化铝; 洗涤附着有核酸的氧化铝; 并从氧化铝中洗脱核酸。
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公开(公告)号：US20050112754A1
公开(公告)日：2005-05-26
申请号：US10476036
申请日：2002-12-05
Applicant: Dae-Sung Yoon , You-Seop Lee , Geun-Bae Lim , Jae-Chang Lee , Moon-Hyuck Jung
Inventor： Dae-Sung Yoon , You-Seop Lee , Geun-Bae Lim , Jae-Chang Lee , Moon-Hyuck Jung
CPC classification number: C12Q1/686 , B01L3/5025 , B01L3/5027 , B01L7/52 , B01L7/54 , B01L2300/1827 , B01L2300/1861 , B01L2300/1883 , C12Q2565/629
Abstract: The present invention provides an apparatus for amplifying a polynucleotide, comprising a substrate, a microflow channel system disposed in the substrate and comprising a sample inlet port, a sample flow channel extending from the sample inlet port, and a polynucleotide polymerization reaction chamber in fluid communication with the sample flow channel, a first insulation groove formed around the reaction chamber, and a means for regulating a temperature of the reaction chamber. Accordingly, a multiple chamber device for amplifying a polynucleotide, comprising multiple polymerization reaction chambers formed in a substrate can be manufactured.
Abstract translation: 本发明提供了一种用于放大多核苷酸的装置，包括基底，设置在基底中的微流道系统，包括样品入口，从样品入口延伸的样品流动通道和流体连通的多核苷酸聚合反应室 与样品流动通道形成在反应室周围形成的第一绝缘槽，以及用于调节反应室温度的装置。 因此，可以制造用于扩增多晶硅的多室装置，其包括形成在基板中的多个聚合反应室。
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公开(公告)号：US08815572B2
公开(公告)日：2014-08-26
申请号：US13001441
申请日：2009-06-22
Applicant: Geun-Bae Lim , Ji-Min Kahng , Jin-Hwa Jung , Ho-Taik Kwon
Inventor： Geun-Bae Lim , Ji-Min Kahng , Jin-Hwa Jung , Ho-Taik Kwon
CPC classification number: C12N15/1003 , B01L3/502 , B01L3/5021 , B01L2200/0631 , B01L2300/0681 , B01L2300/069 , B01L2300/0832
Abstract: The present invention relates to a nucleic acid extracting apparatus, and the nucleic acid extracting apparatus can include a pipe-shaped tube having an open outlet at one side thereof, and a hydrogel supporting member that is provided inside the tube and filters impurities excluding an extraction target material.
Abstract translation: 核酸提取装置技术领域本发明涉及核酸提取装置，核酸提取装置可以包括在其一侧具有开放出口的管状管和设置在管内的水凝胶支撑体，除去除了提取物以外的杂质 目标材料
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29.发明授权Manufacturing method of 3D shape structure having hydrophobic external surface 有权具有疏水性外表面的3D形状结构的制造方法公开(公告)号：US08394283B2
公开(公告)日：2013-03-12
申请号：US12741058
申请日：2008-03-12
Applicant: Dong-Seob Kim , Sang-Min Lee , Woon-Bong Hwang , Kun-Hong Lee , Geun-Bae Lim , Joon-Won Kim , Dong-Hyun Kim , Hyun-Chul Park
Inventor： Dong-Seob Kim , Sang-Min Lee , Woon-Bong Hwang , Kun-Hong Lee , Geun-Bae Lim , Joon-Won Kim , Dong-Hyun Kim , Hyun-Chul Park
IPC: B31D3/00
CPC classification number: C23F1/00 , C25D1/08 , C25D11/005 , C25D11/02
Abstract: The present invention relates to a three-dimensional structure manufacturing method for performing surface treatment processes, and a replication step to provide hydrophobicity on an external surface of the three-dimensional structure. In the manufacturing method, the hydrophobicity may be provided to the external surface of the three-dimensional structure, a high cost device required in the conventional MEMS process is not used, the manufacturing cost is reduced, and the manufacturing process is simplified. In addition, it has been difficult to provide the hydrophobicity on an external surface of a three-dimensional structure having a large surface due to a spatial limitation, but in the exemplary embodiment of the present invention, the hydrophobicity may be provided to the external surface of the three-dimensional structure having a large surface, such as a torpedo, a submarine, a ship, and a vehicle, without the spatial limitation.
Abstract translation: 本发明涉及一种用于进行表面处理工艺的三维结构制造方法以及在三维结构的外表面上提供疏水性的复制步骤。 在制造方法中，可以将疏水性提供给三维结构的外表面，不使用常规MEMS工艺中所需的高成本装置，制造成本降低，制造工艺简化。 此外，由于空间限制，难以在具有大表面的三维结构的外表面上提供疏水性，但是在本发明的示例性实施方案中，可以将疏水性提供给外表面 的具有大表面的三维结构，例如鱼雷，潜艇，船舶和车辆，没有空间限制。
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公开(公告)号：US20110300609A1
公开(公告)日：2011-12-08
申请号：US13001441
申请日：2009-06-22
Applicant: Geun-Bae Lim , Ji-Min Kahng , Jin-Hwa Jung , Ho-Taik Kwon
Inventor： Geun-Bae Lim , Ji-Min Kahng , Jin-Hwa Jung , Ho-Taik Kwon
CPC classification number: C12N15/1003 , B01L3/502 , B01L3/5021 , B01L2200/0631 , B01L2300/0681 , B01L2300/069 , B01L2300/0832
Abstract: The present invention relates to a nucleic acid extracting apparatus, and the nucleic acid extracting apparatus can include a pipe-shaped tube having an open outlet at one side thereof, and a hydrogel supporting member that is provided inside the tube and filters impurities excluding an extraction target material.
Abstract translation: 核酸提取装置技术领域本发明涉及核酸提取装置，核酸提取装置可以包括在其一侧具有开放出口的管状管和设置在管内的水凝胶支撑体，除去除了提取物以外的杂质 目标材料
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