公开(公告)号：US20160216231A1

公开(公告)日：2016-07-28

申请号：US15087020

申请日：2016-03-31

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan

IPC: G01N27/447 ,  G01N33/543 ,  G01N15/10 ,  C12Q1/68

CPC classification number: G01N27/447 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  C12Q1/6806 ,  G01N15/1031 ,  G01N27/44717 ,  G01N27/44721 ,  G01N33/543 ,  G01N35/10 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1081 ,  G01N2035/1034 ,  Y10T436/11 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.

公开(公告)号：US20160108432A1

公开(公告)日：2016-04-21

申请号：US14890451

申请日：2014-05-16

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Srikoundinya Punnamaraju ,  Melissa A. Sandahl ,  John J. Harrington ,  Allen E. Eckhardt ,  Zhishan Hua ,  Michael G. Pollack

IPC: C12N15/87 ,  C12M1/42 ,  C12N13/00

CPC classification number: C12N15/87 ,  C12M33/00 ,  C12M35/02 ,  C12N13/00

Abstract: The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator.

Abstract translation: 本发明提供了一种设计用于在液滴上对细胞进行电穿孔的液滴致动器。 本发明还提供了用于在液滴致动器上的液滴中对细胞进行电穿孔的方法和系统。

公开(公告)号：US20150330943A1

公开(公告)日：2015-11-19

申请号：US14805532

申请日：2015-07-22

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

IPC: G01N27/447

CPC classification number: G01N27/447 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  C12Q1/6806 ,  G01N15/1031 ,  G01N27/44717 ,  G01N27/44721 ,  G01N33/543 ,  G01N35/10 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1081 ,  G01N2035/1034 ,  Y10T436/11 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a method of providing a droplet comprising a single cell is provided, wherein the method includes providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium, dispensing from the droplet to provide a dispensed droplet, determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell, and sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations.

Abstract translation: 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了一种提供包括单个电池的液滴的方法，其中所述方法包括提供液滴，其包含由油介质包围的电润湿液滴致动器上的悬浮液，从液滴分配以提供分配的液滴 确定电润湿液滴致动器上的分配液滴是否包括单个电池，并且基于使用电润湿介导的液滴操作的确定步骤的结果，将分配的液滴分配在电润湿液滴致动器上。

公开(公告)号：US09081007B2

公开(公告)日：2015-07-14

申请号：US14466193

申请日：2014-08-22

Applicant: Advanced Liquid Logic, Inc.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/561 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  B03C1/02 ,  B03C1/30 ,  B03C5/00 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

Abstract translation: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。

公开(公告)号：US20140008222A1

公开(公告)日：2014-01-09

申请号：US14027960

申请日：2013-09-16

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Zhishan Hua ,  Arjun Sudarsan ,  Allen E. Eckhardt

IPC: B01L3/02

CPC classification number: G01N27/44743 ,  B01L3/0268 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/061 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/16 ,  B01L2400/0415 ,  B01L2400/0427

Abstract: A method of loading a droplet actuator is provided. In one embodiment, the method may include, providing: a droplet actuator loaded with a filler fluid; a reservoir comprising a droplet fluid; and a fluid path extending from the reservoir into the droplet actuator; and forcing filler fluid from one locus in the droplet actuator to another locus in the droplet actuator; or out of the droplet actuator; thereby causing droplet fluid to flow through the fluid path and into the droplet actuator.

Abstract translation: 提供了一种装载液滴致动器的方法。 在一个实施例中，该方法可以包括：提供：负载有填充流体的液滴致动器; 包括液滴流体的储存器; 以及从储存器延伸到液滴致动器中的流体路径; 并且迫使填充液从液滴致动器中的一个轨迹到液滴致动器中的另一个轨迹; 或离开液滴致动器; 从而使液滴流体流过流体路径并进入液滴致动器。

公开(公告)号：US12181467B2

公开(公告)日：2024-12-31

申请号：US16455506

申请日：2019-06-27

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/12 ,  G01N27/447 ,  G01N35/00 ,  G01N35/10

Abstract: A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.

公开(公告)号：US11789015B2

公开(公告)日：2023-10-17

申请号：US16948074

申请日：2020-09-01

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Ramakrishna Sista ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen E. Eckhardt

IPC: G01N33/543 ,  B01L3/00 ,  B01F31/65 ,  B01F33/451 ,  B01F33/302 ,  B01F33/3031 ,  G01N1/38 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F31/65 ,  B01F33/3021 ,  B01F33/3031 ,  B01F33/451 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  G01N1/38 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/089 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575 ,  Y10T436/25625

Abstract: Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.

公开(公告)号：US11255809B2

公开(公告)日：2022-02-22

申请号：US16788187

申请日：2020-02-11

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N27/26 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/327 ,  G01N27/416 ,  B01L3/02

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

公开(公告)号：US10809254B2

公开(公告)日：2020-10-20

申请号：US16191270

申请日：2018-11-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Ramakrishna Sista ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen E. Eckhardt

IPC: G01N1/40 ,  G01N1/00 ,  G01N1/10 ,  G01N1/22 ,  G01N1/18 ,  G01N35/00 ,  G01N1/38 ,  B01L3/00 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01F13/08 ,  B01L7/00 ,  G01N35/10

Abstract: Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.

公开(公告)号：US20200209231A1

公开(公告)日：2020-07-02

申请号：US16811789

申请日：2020-03-06

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543 ,  B01F11/00 ,  G01N33/561 ,  C12N15/10 ,  B03C5/00 ,  B03C1/30 ,  B03C1/28 ,  B03C1/02 ,  B01L3/00 ,  B01F13/00

Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.