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    Nanofilter devices using elastomeric micro to nanochannel interfaces and methods based thereon
    21.
    发明授权
    Nanofilter devices using elastomeric micro to nanochannel interfaces and methods based thereon 有权
    使用弹性体微纳通道界面的纳滤器装置和基于此的方法

    公开(公告)号：US08691588B2

    公开(公告)日：2014-04-08

    申请号：US13391508

    申请日：2010-08-20

    申请人： Seung-min Park ,  Yun Suk Huh ,  David Erickson ,  Harold G. Craighead

    发明人： Seung-min Park ,  Yun Suk Huh ,  David Erickson ,  Harold G. Craighead

    IPC分类号： B29C43/44 ,  B29C43/00 ,  B29C59/02

    CPC分类号： G01N21/658 ,  B01D67/0088 ,  B01D71/70 ,  B01D2323/283 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2300/123 ,  B01L2400/0421 ,  B81B2201/058 ,  B81C1/00071 ,  B82Y30/00 ,  G01N33/48721

    摘要： A method is provided for fabricating a nanochannel. The method comprises providing a microchannel and controlling collapse of the microchannel so that it collapses to form a nanochannel of desired dimensions. The method employs a collapsible, flexible material such as the elastomer polydimethylsiloxane (PDMS) to form the nanochannel. A master is provided that is configured to have geometric conditions that promote a desired frequency of microchannel collapse. A collapsible material having a stiffness that also promotes a desired frequency of microchannel collapse is molded on the master. The molded collapsible material is removed from the master and bonded to a base, thereby forming the microchannel, which then collapses (or is collapsed) to form the nanochannel of desired dimensions. Nanofluidic and microfluidic devices comprising complex nanochannel structures and micro to nanochannel transitions are also provided.

    摘要翻译： 提供了制造纳米通道的方法。 该方法包括提供微通道并控制微通道的塌陷，使得其塌缩以形成具有期望尺寸的纳米通道。 该方法采用可折叠的柔性材料，例如弹性体聚二甲基硅氧烷（PDMS）以形成纳米通道。 提供了一个主设备，其配置为具有促进微通道倒塌的期望频率的几何条件。 在主体上模制具有也促进所需频率的微通道塌陷的刚度的可折叠材料。 模制的可折叠材料从主体中取出并结合到基底上，从而形成微通道，然后将其收缩（或折叠）以形成所需尺寸的纳米通道。 还提供了包括复杂纳米通道结构和微到纳通道转换的纳流体和微流体装置。

    Active micromixer using surface acoustic wave streaming
    23.
    发明授权
    Active micromixer using surface acoustic wave streaming 有权
    使用表面声波流动的有源微混合器

    公开(公告)号：US07942568B1

    公开(公告)日：2011-05-17

    申请号：US11155108

    申请日：2005-06-17

    申请人： Darren W. Branch ,  Grant D. Meyer ,  Harold G. Craighead

    发明人： Darren W. Branch ,  Grant D. Meyer ,  Harold G. Craighead

    IPC分类号： B01F11/02

    CPC分类号： B01F11/0266 ,  B01F13/0059 ,  Y10S366/04

    摘要： An active micromixer uses a surface acoustic wave, preferably a Rayleigh wave, propagating on a piezoelectric substrate to induce acoustic streaming in a fluid in a microfluidic channel. The surface acoustic wave can be generated by applying an RF excitation signal to at least one interdigital transducer on the piezoelectric substrate. The active micromixer can rapidly mix quiescent fluids or laminar streams in low Reynolds number flows. The active micromixer has no moving parts (other than the SAW transducer) and is, therefore, more reliable, less damaging to sensitive fluids, and less susceptible to fouling and channel clogging than other types of active and passive micromixers. The active micromixer is adaptable to a wide range of geometries, can be easily fabricated, and can be integrated in a microfluidic system, reducing dead volume. Finally, the active micromixer has on-demand on/off mixing capability and can be operated at low power.

    摘要翻译： 有源微混合器使用在压电衬底上传播的表面声波，优选瑞利波，以在微流体通道中的流体中引起声流。 可以通过将RF激励信号施加到压电基板上的至少一个叉指式换能器来产生表面声波。 活性微混合器可以在低雷诺数流中快速混合静态流体或层流。 活性微混合器没有移动部件（SAW传感器除外），因此比其他类型的有源和无源微混合器更可靠，对敏感流体的损害较小，并且不易受到结垢和通道堵塞的影响。 活性微混合器适用于宽范围的几何形状，可以容易地制造，并且可以集成在微流体系统中，从而减少死体积。 最后，有源微混频器具有按需开/关混合能力，可以低功耗运行。

    METHOD FOR SEQUENCING NUCLEIC ACID MOLECULES
    24.
    发明申请
    METHOD FOR SEQUENCING NUCLEIC ACID MOLECULES 审中-公开
    用于测序核酸分子的方法

    公开(公告)号：US20110111401A1

    公开(公告)日：2011-05-12

    申请号：US12841819

    申请日：2010-07-22

    申请人： Jonas KORLACH ,  Watt W. Webb ,  Michael Levene ,  Stephen Turner ,  Harold G. Craighead ,  Mathieu Foquet

    发明人： Jonas KORLACH ,  Watt W. Webb ,  Michael Levene ,  Stephen Turner ,  Harold G. Craighead ,  Mathieu Foquet

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/143333 ,  C12Q2565/537 ,  C12Q2537/149 ,  C12Q2561/113 ,  C12Q2521/543 ,  C12Q2565/518 ,  C12Q2561/12

    摘要： The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.

    摘要翻译： 本发明涉及对具有多个碱基的靶核酸分子进行测序的方法。 在其原理中，聚合反应中碱添加的时间顺序是在核酸分子上测量的，即核酸聚合酶在待测序的模板核酸分子上的活性被实时跟踪。 通过在碱添加序列的每个步骤中通过核酸聚合酶的催化活性鉴定哪个碱基被掺入靶核酸的生长互补链中来推断该序列。 在靶核酸分子复合物上提供聚合酶，其适于沿着靶核酸分子移动并在活性位点延伸寡核苷酸引物。 多个标记类型的核苷酸类似物在活性位点附近提供，每种可区分类型的核苷酸类似物与靶核酸序列中的不同核苷酸互补。 生长的核酸链通过使用聚合酶延伸到活性位点处的核酸链的核苷酸类似物，其中加入的核苷酸类似物与活性位点上的靶核酸的核苷酸互补。 鉴定作为聚合步骤的结果添加到寡核苷酸引物中的核苷酸类似物。 重复提供标记的核苷酸类似物，聚合生长的核酸链和鉴定添加的核苷酸类似物的步骤，使得核酸链进一步延长并确定靶核酸的序列。

    Method for sequencing nucleic acid molecules
    27.
    发明授权
    Method for sequencing nucleic acid molecules 有权

    公开(公告)号：US07056676B2

    公开(公告)日：2006-06-06

    申请号：US11015138

    申请日：2004-12-16

    申请人： Jonas Korlach ,  Watt W. Webb ,  Michael Levene ,  Stephen Turner ,  Harold G. Craighead ,  Mathieu Foquet

    发明人： Jonas Korlach ,  Watt W. Webb ,  Michael Levene ,  Stephen Turner ,  Harold G. Craighead ,  Mathieu Foquet

    IPC分类号： C12Q1/68 ,  C12M3/00 ,  G01N21/00 ,  C07H21/02

    CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/143333 ,  C12Q2565/537 ,  C12Q2537/149 ,  C12Q2561/113 ,  C12Q2521/543 ,  C12Q2565/518 ,  C12Q2561/12

    摘要： The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.

    Sub-micrometer fluidic channel for measuring photon emitting entities
    28.
    发明授权
    Sub-micrometer fluidic channel for measuring photon emitting entities 有权
    用于测量光子发射实体的亚微米流体通道

    公开(公告)号：US08890323B2

    公开(公告)日：2014-11-18

    申请号：US12716087

    申请日：2010-03-02

    申请人： Samuel M. Stavis ,  Joshua B. Edel ,  Kevan T. Samiee ,  Harold G. Craighead

    发明人： Samuel M. Stavis ,  Joshua B. Edel ,  Kevan T. Samiee ,  Harold G. Craighead

    IPC分类号： G01R31/26 ,  G01J3/30 ,  B82Y30/00 ,  B82Y15/00 ,  B82Y10/00 ,  B82Y5/00 ,  G01N33/58 ,  B82Y20/00 ,  A61K49/00

    CPC分类号： A61K49/0067 ,  A61K49/0056 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N33/588 ,  Y10S438/962 ,  Y10S977/70 ,  Y10S977/774

    摘要： A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.

    摘要翻译： 使用在约500nm正方形横截面的熔融二氧化硅中制造的纳米流体通道来分离，检测和识别各种量子点缀合物。 该通道可以快速检测溶液中的每个荧光实体。 使用选定波长的激光来激发多种量子点和有机分子，并且发射光谱被解析而没有显着的信号抑制。 然后将量子点与有机分子缀合并检测以证明有效的多色检测。 PCH用于分析重合检测并表征结合程度。 使用小的流体通道检测量子点作为荧光标记被证明是多重单分子研究的有效技术。 单分子结合事件的检测具有多种应用，包括高通量免疫测定。

    Multiplexed Electrospray Deposition Apparatus
    30.
    发明申请
    Multiplexed Electrospray Deposition Apparatus 有权

    公开(公告)号：US20130045335A1

    公开(公告)日：2013-02-21

    申请号：US13657220

    申请日：2012-10-22

    申请人： Parijat Bhatnagar ,  Harold G. Craighead

    发明人： Parijat Bhatnagar ,  Harold G. Craighead

    IPC分类号： B05B5/025 ,  B01J19/08

    CPC分类号： B05B5/0255 ,  B05B5/005 ,  B05B5/025 ,  B05B5/08

    摘要： Multiplexed electrospray deposition apparatus capable of delivering picoliter volumes of one or more substances is disclosed. The apparatus may include a unitary planar dispenser etched from a silicon wafer through microfabrication or micromachining technology. The apparatus may be used as a deposition tool for making protein microarrays in a noncontact mode. Upon application of potential difference in the range of 7-9 kV, the substances may be dispensed directly, not through a collimating mask, onto a substrate with microhydrogel features functionalized with an anchoring agent.