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21.发明授权Droplet actuator devices, configurations, and methods for improving absorbance detection 有权液滴致动器装置，配置和改善吸光度检测的方法公开(公告)号：US08208146B2
公开(公告)日：2012-06-26
申请号：US12529025
申请日：2008-03-13
CPC分类号： G01N21/0303 , G01N21/031 , G01N2021/035 , G01N2021/036 , G01N2201/08
摘要： Devices, configurations and methods for improving absorbance detection are provided. For example, methods and devices are provided for determining the absorbance of a droplet, e.g., a droplet on a droplet actuator, by providing an elongated light path through the droplet.
摘要翻译： 提供了用于改善吸光度检测的装置，配置和方法。 例如，提供了通过提供穿过液滴的细长光路来确定液滴（例如液滴致动器上的液滴）的吸光度的方法和装置。
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公开(公告)号：US20120018306A1
公开(公告)日：2012-01-26
申请号：US13249425
申请日：2011-09-30
CPC分类号： B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
摘要： Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.
摘要翻译： 提供样品处理液滴致动器，系统和方法。 根据一个实施例，提供了一种包括液滴微致动器的冲压装置，其包括：（a）包括用于在其表面上输送液滴的控制电极的路径或网络的第一板; （b）相对于第一板以基本上平行的方向安装的第二板，提供板之间的内部容积，第二板包括一个或多个冲压端口，用于将一些部分或全部液滴从内部容积输送到 外部位置; （c）用于将流体引入板之间的内部容积的端口; 和（d）对应于控制电极的路径或网络的参考电极的路径或网络。 还提供了包括冲压装置在内的相关系统和方法。
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公开(公告)号：US20110097763A1
公开(公告)日：2011-04-28
申请号：US12991966
申请日：2009-05-13
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12P19/34
CPC分类号： B01L7/525 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L2200/027 , B01L2200/0668 , B01L2200/10 , B01L2300/0803 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/088 , B01L2300/0883 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/043 , C12Q1/686 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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24.发明申请Droplet Actuator Configurations and Methods of Conducting Droplet Operations 审中-公开液滴执行器配置和执行液滴操作的方法公开(公告)号：US20100270156A1
公开(公告)日：2010-10-28
申请号：US12682830
申请日：2008-12-23
申请人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Paik
发明人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Paik
IPC分类号： C07K1/26
CPC分类号： B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
摘要： A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
摘要翻译： 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。
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公开(公告)号：US07763471B2
公开(公告)日：2010-07-27
申请号：US11839762
申请日：2007-08-16
IPC分类号： G01N33/00
CPC分类号： G01N23/20 , B01L3/06 , B01L3/502792 , B01L2200/16 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0427 , Y10T436/25
摘要： Methods of using protein crystallization droplet actuators are provided. Protein sample droplets and reagent droplets are dispensed, transported, and merged to yield an array of crystallization conditions by electrowetting droplet operations in a gap comprising oil filler fluid. The oil filler fluid is doped with a surfactant that enhances droplet operations using the protein sample.
摘要翻译： 提供了使用蛋白质结晶液滴致动器的方法。 蛋白质样品液滴和试剂液滴被分配，运输和合并，以通过在包含填充油的间隙中的电润湿液滴操作产生一系列结晶条件。 油填充液掺杂有使用蛋白质样品增强液滴操作的表面活性剂。
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公开(公告)号：US20100032293A1
公开(公告)日：2010-02-11
申请号：US12531809
申请日：2008-04-10
CPC分类号： B01L3/502761 , B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/16 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/088 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , G01N27/447
摘要： The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.
摘要翻译： 本发明提供了用于在液滴致动器中分配液滴的结构的方法和方法的非限制性实例。 本发明的液滴致动器结构和方法与现有技术的液滴致动器相比表现出许多优点。 在各种实施例中，与现有的液滴致动器相比，本发明的结构和方法尤其提供了改进的效率，吞吐量，可扩展性和/或液滴均匀性。 此外，在一些实施例中，液滴致动器提供用于加载和/或卸载流体和/或液滴的改进方法的构造。 在其他实施例中，液滴致动器提供用于以基本上同时和/或基本顺序的方式装载多个流体储存器的流体加载构造。
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公开(公告)号：US20080230386A1
公开(公告)日：2008-09-25
申请号：US11839786
申请日：2007-08-16
IPC分类号： B01J19/08
CPC分类号： B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
摘要： Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.
摘要翻译： 提供样品处理液滴致动器，系统和方法。 根据一个实施例，提供了一种包括液滴微致动器的冲压装置，其包括：（a）包括用于在其表面上输送液滴的控制电极的路径或网络的第一板; （b）相对于第一板以基本上平行的方向安装的第二板，提供板之间的内部容积，第二板包括一个或多个冲压端口，用于将一些部分或全部液滴从内部容积输送到 外部位置; （c）用于将流体引入板之间的内部容积的端口; 和（d）对应于控制电极的路径或网络的参考电极的路径或网络。 还提供了包括冲压装置在内的相关系统和方法。
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公开(公告)号：US20070243634A1
公开(公告)日：2007-10-18
申请号：US11639736
申请日：2006-12-15
IPC分类号： G01N33/543
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供与物质浓度降低的表面接触的液滴的方法，其中所述方法包括：（a）提供液滴微致动器，其包括与包含起始浓度的液滴的表面 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US20070242105A1
公开(公告)日：2007-10-18
申请号：US11639594
申请日：2006-12-15
IPC分类号： B41J2/045
CPC分类号： B01L3/502792 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , B03C5/02 , B81B1/00 , G01N27/447 , G01N2035/1046
摘要： The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.
摘要翻译： 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了液滴微致动器，其包括：（a）第一基板，其包括被配置用于在所述基板的表面上进行液滴操作的电极; （b）第二衬底，其与衬底的表面间隔足以限定第一衬底和第二衬底之间的内部空间的距离，其中所述距离足以容纳设置在第一衬底上的空间中的液滴; 和（c）布置在内部体积中并且以允许使用电极对液滴进行液滴操作的方式相对于电极布置的液滴。
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公开(公告)号：US09675972B2
公开(公告)日：2017-06-13
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
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