公开(公告)号：US09932624B2

公开(公告)日：2018-04-03

申请号：US14612183

申请日：2015-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12P19/34 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1003

Abstract: A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided.

公开(公告)号：US20180089366A1

公开(公告)日：2018-03-29

申请号：US15679261

申请日：2017-08-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zheng ZHANG ,  Fiona HYLAND ,  Sowmi UTIRAMERUR

IPC: G06F19/22

CPC classification number: G16B30/00

Abstract: Systems, methods, and computer program products for aligning a fragment sequence to a target sequencing. The alignment is allowed at most one gap, such as an insertion or a deletion. In some embodiments, both a gapped alignment and an ungapped alignment can be produced. A selection can be made between the gapped alignment and the ungapped alignment based on a quality value for each alignment.

公开(公告)号：US09926609B2

公开(公告)日：2018-03-27

申请号：US14978243

申请日：2015-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei Ji ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US20180071700A1

公开(公告)日：2018-03-15

申请号：US15803327

申请日：2017-11-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Clinton C. Staheli ,  Brandon M. Knudsen ,  Trevor G. Williams ,  Steven R. Kjar

IPC: B01F7/00 ,  C12M1/06 ,  C12M1/00 ,  B01F15/00 ,  B01F7/20 ,  B01F7/16

CPC classification number: B01F7/00683 ,  B01F7/00058 ,  B01F7/001 ,  B01F7/00158 ,  B01F7/00291 ,  B01F7/00316 ,  B01F7/00633 ,  B01F7/00675 ,  B01F7/1695 ,  B01F7/20 ,  B01F15/00668 ,  B01F15/00681 ,  B01F15/00707 ,  B01F15/0085 ,  C12M23/14 ,  C12M23/52 ,  C12M27/02

Abstract: A fluid mixing system includes a container, such as a flexible bag, bounding a compartment. A flexible drive line is disposed within the compartment, the drive line having a first end rotatably connected to a first end of the container and an opposing second end rotatably connected to a second end of the container. At least one mixing element, such as an impeller, can be coupled with the flexible drive line. Rotation of the drive line facilitates rotation of the impeller within the container.

公开(公告)号：US20180060481A1

公开(公告)日：2018-03-01

申请号：US15691683

申请日：2017-08-30

Applicant: Life Technologies Corporation

Inventor： Michael BEVILACQUA ,  Danute Bankaitis-Davis ,  John Cheronis ,  Victor Tryon

IPC: G06F19/18 ,  G06F19/00 ,  G06F19/20 ,  A61B5/0205 ,  A61B5/145 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/574 ,  G01N33/68 ,  A61B5/021 ,  A61B5/024 ,  A61B5/01 ,  C12Q1/70

CPC classification number: G16B20/00 ,  A61B5/01 ,  A61B5/02055 ,  A61B5/021 ,  A61B5/024 ,  A61B5/145 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q1/6893 ,  C12Q1/6895 ,  C12Q1/701 ,  C12Q2600/158 ,  G01N33/5088 ,  G01N33/57415 ,  G01N33/6803 ,  G01N33/6863 ,  G01N33/6869 ,  G01N33/6893 ,  G01N2800/042 ,  G16B25/00 ,  G16H50/20 ,  Y02A90/26

Abstract: A method is provided in various embodiments for determining a profile data set for a subject with infectious disease or inflammatory conditions related to infectious disease based on a sample from the subject, wherein the sample provides a source of RNAs. The method includes using amplification for measuring the amount of RNA corresponding to at least 2 constituents from Table 1. The profile data set comprises the measure of each constituent, and amplification is performed under measurement conditions that are substantially repeatable.

公开(公告)号：US20180057814A1

公开(公告)日：2018-03-01

申请号：US15691721

申请日：2017-08-30

Applicant: Life Technologies Corporation

Inventor： Christopher BURNETT ,  Nitin Puri ,  Susan Magdaleno ,  Alexander Vlassov

IPC: C12N15/113 ,  A61K31/713 ,  C12N15/11

CPC classification number: C12N15/113 ,  A61K31/713 ,  A61K47/50 ,  C12N15/111 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/332 ,  C12N2310/3515 ,  C12N2310/3519 ,  C12N2310/51 ,  C12N2310/531 ,  C12N2330/31

Abstract: The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.

公开(公告)号：US09890424B2

公开(公告)日：2018-02-13

申请号：US14742404

申请日：2015-06-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  Todd Roswech ,  Jon A. Hoshizaki ,  Albert L. Carrillo ,  James A. Ball

IPC: C12Q1/68 ,  G01N35/10 ,  G01N1/00 ,  B01F5/06 ,  B01F11/00 ,  B01F1/00 ,  B01L3/00 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  B01F1/0027 ,  B01F5/0683 ,  B01F11/0048 ,  B01F11/0071 ,  B01L3/502 ,  B01L3/505 ,  B01L3/527 ,  B01L3/563 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  C12Q1/6806 ,  G01N27/4145 ,  G01N35/1002 ,  G01N35/1095 ,  Y10T436/11 ,  Y10T436/143333

Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

公开(公告)号：US20180031545A1

公开(公告)日：2018-02-01

申请号：US15532271

申请日：2015-12-03

Applicant: Life Technologies Corporation

Inventor： James STRAY ,  Shaheer KHAN

IPC: G01N33/53 ,  G01N27/447 ,  G01N33/58 ,  G06F19/00 ,  C12Q1/40 ,  C12Q1/68 ,  G01N33/533

CPC classification number: G01N33/5308 ,  C12Q1/40 ,  C12Q1/6876 ,  G01N27/447 ,  G01N27/44726 ,  G01N30/00 ,  G01N30/34 ,  G01N33/533 ,  G01N33/58 ,  G01N33/582 ,  G01N33/66 ,  G01N2030/8813 ,  G01N2030/8836 ,  G01N2333/924 ,  G01N2400/00 ,  G01N2400/22 ,  G01N2458/10 ,  G01N2800/26 ,  G06F19/70 ,  G06F19/709

Abstract: Methods, systems, compositions and kits are described for detecting cleaved glycans from a glycoconjugate. After labeling the glycan with a nucleic acid charged oligomer described herein, the labeled glycans can be separated under the influence of an electric field or based on their detectable tag and identified.

公开(公告)号：US09879283B2

公开(公告)日：2018-01-30

申请号：US14879872

申请日：2015-10-09

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Namritha Ravinder ,  Korbinian Heil ,  Yizhu Guo ,  Xiquan Liang ,  Robert Potter ,  Sanjay Kumar

IPC: C12Q1/68 ,  C12N15/90 ,  C12N15/10 ,  C12P19/34 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/3519 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/143

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.

公开(公告)号：US09868826B2

公开(公告)日：2018-01-16

申请号：US15200533

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Talha M. Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08J3/075 ,  C08F220/58 ,  C08F8/12 ,  C08F220/70 ,  C08F230/08

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.