公开(公告)号：US20080226504A1

公开(公告)日：2008-09-18

申请号：US11861324

申请日：2007-09-26

Applicant: Jong-myeon PARK ,  Nam Huh ,  Jeong-gun Lee ,  Kyu-youn Hwang

Inventor： Jong-myeon PARK ,  Nam Huh ,  Jeong-gun Lee ,  Kyu-youn Hwang

IPC: B04B5/10

CPC classification number: B01L3/50273 ,  B01L3/502738 ,  B01L2300/0803 ,  B01L2400/0442 ,  B01L2400/0487 ,  B01L2400/0677 ,  F04B19/006 ,  F04B19/24 ,  F04F1/06

Abstract: Provided are a pump unit and a centrifugal microfluidic system including the pump unit. The pump unit includes a gas generating agent. The gas generating agent includes a plurality of heating particles generating heat by absorbing energy and one of a sublimation material, azide, and azo compound mixed with the heating particles. When energy is supplied to the gas generating agent, the gas generating agent generates gas due to heat emitted from the heating particles to increase an air pressure around the gas generation agent so that the fluid is moved by the increased air pressure.

Abstract translation: 提供了包括泵单元的泵单元和离心微流体系统。 泵单元包括气体发生剂。 气体发生剂包括通过吸收能量产生热的多个加热颗粒和与加热颗粒混合的升华材料，叠氮化物和偶氮化合物之一。 当向气体发生剂供应能量时，气体发生剂由加热颗粒发出的热量产生气体，从而增加气体发生剂周围的空气压力，使得流体通过增加空气压力而移动。

公开(公告)号：US20080081357A1

公开(公告)日：2008-04-03

申请号：US11733846

申请日：2007-04-11

Applicant: Young-nam KWON ,  Jeong-gun LEE ,  Jeong-hee LEE ,  Ju-chul PARK ,  Chang-eun YOO

Inventor： Young-nam KWON ,  Jeong-gun LEE ,  Jeong-hee LEE ,  Ju-chul PARK ,  Chang-eun YOO

IPC: C12P19/34 ,  C12M1/42 ,  C12N13/00

CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/1861 ,  B82Y5/00 ,  B82Y30/00 ,  C12M47/06 ,  C12N1/066 ,  C12N13/00 ,  C12N15/1006

Abstract: Provided herein are a method and an apparatus for isolating nucleic acids from cells. The method comprises introducing carbon nanotubes (CNTs) and silica beads into a solution containing the cells, irradiating the solution with a laser beam disrupt the cells releasing the nucleic acids from the disrupted cells, thereby binding the nucleic acids to the silica beads, and adding a nucleic acid eluting solution to the silica beads to which the nucleic acids are bound, to elute the nucleic acids from the silica beads.

Abstract translation: 本文提供了用于从细胞中分离核酸的方法和装置。 该方法包括将碳纳米管（CNT）和二氧化硅珠粒引入到含有细胞的溶液中，用激光束照射溶液从而破坏从破碎的细胞中释放核酸的细胞，从而将核酸与二氧化硅珠结合，并加入 将与核酸结合的二氧化硅珠粒的核酸洗脱溶液从二氧化硅珠洗脱出来。

公开(公告)号：US20080058991A1

公开(公告)日：2008-03-06

申请号：US11835461

申请日：2007-08-08

Applicant: Jeong-gun LEE ,  Yoon-kyoung Cho ,  Beom-seok Lee ,  Jong-myeon Park

Inventor： Jeong-gun LEE ,  Yoon-kyoung Cho ,  Beom-seok Lee ,  Jong-myeon Park

IPC: G05B15/00 ,  B01J19/00 ,  G01N21/01

CPC classification number: B01L3/502715 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/14 ,  B01L2300/0806 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0032 ,  F16K2099/0084 ,  F16K2099/0086 ,  G01N35/00069 ,  Y10T436/11 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Provided are an apparatus and a method of controlling a microfluidic system, and the microfluidic system. The apparatus of controlling the microfluidic system includes a central control block controlling an operation of the microfluidic system, a rotator control block controlling a rotator, a position control block controlling the position of a moving unit, the moving unit moving to a position of the microfluidic structure, and a radiation energy source control block controlling energy of a radiation energy source, the radiation energy source using an electromagnetic wave to scan over a position of the microfluidic structure. Such a configuration allows effective control of a miniaturized portable microfluidic system.

Abstract translation: 提供了一种控制微流体系统和微流体系统的装置和方法。 控制微流体系统的装置包括控制微流体系统的操作的中央控制块，控制旋转器的旋转体控制块，控制移动单元的位置的位置控制块，移动到微流体位置的移动单元 结构以及辐射能量源控制块，其控制辐射能源的能量，所述辐射能源使用电磁波扫描微流体结构的位置。 这种配置允许有效地控制微型便携式微流体系统。

公开(公告)号：US20080029158A1

公开(公告)日：2008-02-07

申请号：US11766806

申请日：2007-06-22

Applicant: Jong-myeon Park ,  Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Sung-woo Hong

Inventor： Jong-myeon Park ,  Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Sung-woo Hong

IPC: F16K17/40 ,  F16K17/34

CPC classification number: F16K99/0019 ,  B01L3/502738 ,  B01L2300/0803 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0032 ,  F16K99/0034 ,  F16K99/004 ,  F16K2099/0084 ,  Y10T137/1827 ,  Y10T137/2196 ,  Y10T137/6606 ,  Y10T137/7755

Abstract: Provided are a valve unit and a reaction apparatus having the valve unit. The valve unit includes a phase transition material, which melts and expands upon an application of the electromagnetic waves to the valve filler, and the valve filler is directed into the channel through the connection passage and closes the channel. The valve unit also includes heat generation particles, which are dispersed in the phase transition material and generate heat upon an application of electromagnetic wave energy.

Abstract translation: 提供具有阀单元的阀单元和反应装置。 阀单元包括相变材料，其在将电磁波施加到阀填充器时熔化和膨胀，并且阀填料通过连接通道引导到通道中并关闭通道。 阀单元还包括发散颗粒，其分散在相变材料中并且在施加电磁波能量时产生热量。

公开(公告)号：US20080017736A1

公开(公告)日：2008-01-24

申请号：US11781714

申请日：2007-07-23

Applicant: Beom-seok LEE ,  Jeong-gun LEE ,  Hye-jung CHO

Inventor： Beom-seok LEE ,  Jeong-gun LEE ,  Hye-jung CHO

IPC: B05B5/00

CPC classification number: B05B5/0255 ,  B01J2219/00367 ,  B01J2219/00369 ,  B01J2219/00371 ,  B01L3/0268 ,  B01L2300/0838 ,  B01L2300/161 ,  B01L2400/027 ,  B01L2400/0406 ,  B01L2400/0487 ,  B05B1/14 ,  B05B5/0533 ,  B41J2/06

Abstract: Provided is a droplet dispensing device having a nonconductive capillary nozzle. The droplet dispensing device comprises: a nonconductive capillary nozzle disposed in a downward position; a pump connected with the nonconductive capillary nozzle through a hermetically sealed fluid tube and generating a negative pressure to decrease the influence of gravity on a solution within the nonconductive capillary nozzle and the fluid tube; and an open circuit voltage supplier applying a voltage to the solution. The droplet dispensing device supplies the solution by capillary force to regularly maintain the shape of a droplet surface in the tip of the nonconductive capillary nozzle without using a separate driving device.

Abstract translation: 提供了具有非导电毛细管喷嘴的液滴分配装置。 液滴分配装置包括：设置在向下位置的非导电毛细管喷嘴; 泵，其通过气密密封的流体管与非导电毛细管喷嘴连接并产生负压，以减少重力对非导电毛细管喷嘴和流体管内的溶液的影响; 以及向该溶液施加电压的开路电压供给器。 液滴分配装置通过毛细管力提供溶液以在不使用单独的驱动装置的情况下规定地保持非导电毛细管喷嘴的尖端中的液滴表面的形状。

公开(公告)号：US20070284254A1

公开(公告)日：2007-12-13

申请号：US11754523

申请日：2007-05-29

Applicant: Young-kyoung Cho ,  Su-hyeon Kim ,  Chin-sung Park ,  Kyu-sang Lee ,  Jeong-gun Lee

Inventor： Young-kyoung Cho ,  Su-hyeon Kim ,  Chin-sung Park ,  Kyu-sang Lee ,  Jeong-gun Lee

IPC: B03C5/02

CPC classification number: B03C5/005 ,  B03C5/026 ,  B03C2201/26

Abstract: An apparatus separating a polarizable analyte using dielectrophoresis includes a vessel including a membrane having a plurality of nano- to micro-sized pores, the membrane disposed inside the vessel, electrodes generating spatially non-uniform electric fields in the nano- to micro-sized pores of the membrane when an AC voltage is applied to the electrodes, and a power source applying the AC voltage to the electrodes, wherein a sectional area of the pores varies along a depth of the pores. A method of separating a polarizable material uses the apparatus.

Abstract translation: 使用介电电泳分离可极化分析物的装置包括容器，其包括具有多个纳米至微米尺寸孔的膜，所述膜设置在容器内部，电极在纳米至微孔中产生空间上不均匀的电场 当向电极施加AC电压时，将膜施加到膜上，并且向电极施加AC电压的电源，其中孔的截面积沿着孔的深度而变化。 分离可极化材料的方法使用该装置。

公开(公告)号：US20070134809A1

公开(公告)日：2007-06-14

申请号：US11610938

申请日：2006-12-14

Applicant: Yoon-kyoung CHO ,  Jeong-gun LEE ,  Sung-young JEONG

Inventor： Yoon-kyoung CHO ,  Jeong-gun LEE ,  Sung-young JEONG

IPC: G01N1/10

CPC classification number: B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/1861 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L2400/0439 ,  C12M47/02 ,  C12M47/04 ,  C12M47/06 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A microfluidic device for the concentration and lysis of cells or viruses and a method of concentrating and lysing cells or viruses using the microfluidic device include: magnetic beads, a reaction chamber in which the magnetic beads are accommodated and a laser source. The reaction chamber includes a plurality of electrodes which cross each other and are separated by a dielectric to generate an electric field and a vibrating part agitating the magnetic beads in the chamber. The laser source radiates a laser onto the magnetic beads in the reaction chamber.

Abstract translation: 用于细胞或病毒的浓缩和裂解的微流体装置以及使用微流体装置浓缩和裂解细胞或病毒的方法包括：磁珠，容纳磁珠的反应室和激光源。 反应室包括彼此交叉并被电介质隔开以产生电场的多个电极以及搅拌室中的磁珠的振动部分。 激光源将激光辐射到反应室中的磁珠上。

公开(公告)号：US20060255267A1

公开(公告)日：2006-11-16

申请号：US11418602

申请日：2006-05-05

Applicant: Jeong-gun Lee ,  Jeong-woo Choi ,  Nam Huh ,  Hye-jung Cho

Inventor： Jeong-gun Lee ,  Jeong-woo Choi ,  Nam Huh ,  Hye-jung Cho

IPC: G21K7/00

CPC classification number: B01L3/0244 ,  B01J2219/0036 ,  B01J2219/00378 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01L3/0241 ,  B01L2300/0819

Abstract: Provided is a device for printing a biomolecule solution onto a substrate using an electrohydrodynamic (EHD) effect. The device can deposit drops of the biomolecule solution with small size and volume and print more of the biomolecule solution onto a substrate, thereby enabling the manufacture of a high density biochip. The device includes a first electric field forming electrode that is shaped like a needle, is made of a conductive material, is disposed vertically, and includes an accommodating area in which the biomolecule solution is accommodated and an outlet formed on a bottom end of the accommodating area through which the biomolecule solution is discharged; a substrate disposed below the first electric field forming electrode, and including a target surface onto which the biomolecule solution discharged from the outlet of the first electric field forming electrode is deposited; a second electric field forming electrode made of a conductive material and disposed below the first electric field forming electrode; and a voltage applying device which is electrically connected to the first and second electric field forming electrodes to apply a voltage between the first and second electric field forming electrodes so that an electric field is generated around the biomolecule solution suspended in the outlet, wherein, due to the interaction between the electric field and a difference between dielectric constants of the biomolecule solution having a free surface and the surrounding atmosphere, the electric force acts inward on the biomolecule solution, thereby causing a predetermined amount of the biomolecule solution to drop onto the target surface of the substrate.

Abstract translation: 提供了使用电动力学（EHD）效应将生物分子溶液印刷到基底上的装置。 该装置可以沉积小尺寸和体积的生物分子溶液，并将更多的生物分子溶液印刷到基底上，从而能够制造高密度生物芯片。 该装置包括成形为针状的第一电场形成电极，由导电材料制成，垂直设置，并且包括其中容纳有生物分子溶液的容纳区域和形成在容纳物的底端的出口 生物分子溶液排出的区域; 设置在所述第一电场形成电极下方的基板，并且包括从所述第一电场形成用电极的出口排出的生物分子溶液沉积在其上的目标表面; 由导电材料制成并设置在第一电场形成电极下方的第二电场形成电极; 以及电压施加装置，其电连接到第一和第二电场形成电极，以在第一和第二电场形成电极之间施加电压，使得在悬浮在出口中的生物分子溶液周围产生电场，其中，由于 对于电场与具有自由表面和周围大气的生物分子溶液的介电常数之间的差异的相互作用，电力向内作用于生物分子溶液，从而使预定量的生物分子溶液落到目标上 基板的表面。

公开(公告)号：US07090977B2

公开(公告)日：2006-08-15

申请号：US10282251

申请日：2002-10-29

Applicant: Kyu-Sik Yun ,  Jeong-Gun Lee ,  Je-Kyun Park ,  Su-Hyeon Kim ,  Sang-Eun Lee

Inventor： Kyu-Sik Yun ,  Jeong-Gun Lee ,  Je-Kyun Park ,  Su-Hyeon Kim ,  Sang-Eun Lee

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  Y10T436/143333 ,  C12Q2563/173

Abstract: Disclosed is a method for detecting nucleic acid hybridization by using intercalator binding to hybridized nucleic acid, wherein oxidation-reduction of transition metallic complex is induced to cause electrochemiluminescence, thereby providing a method for detecting nucleic acid hybridization without a special labeling.

Abstract translation: 公开了通过使用嵌入剂与杂交核酸结合来检测核酸杂交的方法，其中诱导过渡金属络合物的氧化还原引起电化学发光，从而提供用于检测核酸杂交而不进行特殊标记的方法。

公开(公告)号：US20060118417A1

公开(公告)日：2006-06-08

申请号：US11281226

申请日：2005-11-16

Applicant: Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

Inventor： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

IPC: C07H21/04

CPC classification number: C07H21/04

Abstract: Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.

Abstract translation: 提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。 根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。