公开(公告)号：US06828432B2

公开(公告)日：2004-12-07

申请号：US09975059

申请日：2001-10-11

申请人： Chad A. Mirkin ,  Robert L. Letsinger ,  Robert C. Mucic ,  James J. Storhoff ,  Robert Elghanian ,  Thomas A. Taton

发明人： Chad A. Mirkin ,  Robert L. Letsinger ,  Robert C. Mucic ,  James J. Storhoff ,  Robert Elghanian ,  Thomas A. Taton

IPC分类号： C07H2100

CPC分类号： C07H21/00 ,  B01J2219/00274 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B82B1/00 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00 ,  C07B2200/11 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6839 ,  C40B40/06 ,  Y10S977/924 ,  C12Q2563/155 ,  C12Q2563/143 ,  C12Q2537/143 ,  C12Q2525/197 ,  C12Q2565/625 ,  C12Q2537/125 ,  C12Q2525/161

摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

公开(公告)号：US06767702B2

公开(公告)日：2004-07-27

申请号：US09760500

申请日：2001-01-12

申请人： Chad A. Mirkin ,  Robert L. Letsinger ,  Robert C. Mucic ,  James J. Storhoff ,  Robert Elghanian ,  Thomas A. Taton ,  Viswanadham Garimella ,  Zhi Li

发明人： Chad A. Mirkin ,  Robert L. Letsinger ,  Robert C. Mucic ,  James J. Storhoff ,  Robert Elghanian ,  Thomas A. Taton ,  Viswanadham Garimella ,  Zhi Li

IPC分类号： C12P168

CPC分类号： B82Y30/00 ,  B01J2219/00274 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B82B1/00 ,  B82Y15/00 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6839 ,  C40B40/00 ,  C40B40/06 ,  Y10S977/924 ,  C12Q2563/155 ,  C12Q2563/143 ,  C12Q2537/143 ,  C12Q2525/197 ,  C12Q2525/161 ,  C12Q2565/625 ,  C12Q2537/125

摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

公开(公告)号：US20030059806A1

公开(公告)日：2003-03-27

申请号：US10162164

申请日：2002-06-03

申请人： Science & Technology Corporation @ UNM

发明人： Cossette Wheeler ,  Cheri Goodall

IPC分类号： C12Q001/70 ,  C12Q001/68 ,  C07H021/04

CPC分类号： C12Q1/708 ,  C12Q1/6834 ,  C12Q2565/625

摘要： The present invention provides probes for HPV types 61, 62, 64, 67, 69, 70, 71, 72, CP6108, CP8304, of IS39. The present invention also provides an assay incorporating one or more of these probes. In addition, the present invention provides a method to detect one or more of HPV types 61, 62, 64, 67, 69, 70, 71, 72, CP6108, CP8304, or IS39, and subtypes and variants thereof.

摘要翻译： 本发明提供了IS39的HPV型61,62,64,67,69,70,71,72，CP6108，CP8304的探针。 本发明还提供了包含这些探针中的一种或多种的测定法。 此外，本发明提供了检测HPV类型61,62,64,67,69,70,71,72，CP6108，CP8304或IS39及其亚型及变体中的一种或多种的方法。

公开(公告)号：US20020177144A1

公开(公告)日：2002-11-28

申请号：US10035822

申请日：2001-12-27

发明人： Jose Remacle ,  Isabelle Alexandre ,  Yves Houbion

IPC分类号： C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  C12M001/34

CPC分类号： B01L3/545 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00536 ,  B01J2219/0054 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00704 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/0074 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/12 ,  B01L2300/0806 ,  B01L2300/0861 ,  B82Y30/00 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B70/00 ,  G01N33/54366 ,  G01N33/54373 ,  G01N35/00069 ,  G01N2035/00158 ,  C12Q2565/625

摘要： The present invention is related to a method for the detection and/or the quantification of a target molecule by its binding with a non-cleavable capture molecule fixed on the surface of a disc comprising registered data. The present invention is also related to a disc having fixed upon its surface a non-cleavable capture molecule, to its preparation process, and to a diagnostic and/or reading device of said disc or comprising said disc.

摘要翻译： 本发明涉及通过其与固定在包含登记数据的盘的表面上的不可裂解的捕获分子结合来检测和/或定量靶分子的方法。 本发明还涉及其表面上固定有不可切割的捕获分子，其制备过程以及所述盘的诊断和/或读取装置或包括所述盘的盘。

公开(公告)号：US20020155458A1

公开(公告)日：2002-10-24

申请号：US09967409

申请日：2001-09-28

申请人： Nanosphere, Inc.

发明人： Chad A. Mirkin ,  Robert L. Letsinger ,  Robert C. Mucic ,  James J. Storhoff ,  Robert Elghanian ,  Thomas A. Taton

IPC分类号： C12Q001/68

CPC分类号： B82Y30/00 ,  B01J2219/00274 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B82B1/00 ,  B82Y15/00 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6839 ,  C40B40/06 ,  Y02A50/57 ,  Y10S977/924 ,  C12Q2563/155 ,  C12Q2563/143 ,  C12Q2537/143 ,  C12Q2525/197 ,  C12Q2565/625 ,  C12Q2537/125 ,  C12Q2525/161

摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

公开(公告)号：US20020146720A1

公开(公告)日：2002-10-10

申请号：US09961949

申请日：2001-09-20

申请人： Nanosphere, Inc.

发明人： Chad A. Mirkin ,  Robert L. Letsinger ,  Robert C. Mucic ,  James J. Storhoff ,  Robert Elghanian ,  Thomas A. Taton

IPC分类号： C12Q001/68

CPC分类号： B82Y30/00 ,  B01J2219/00274 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B82B1/00 ,  B82Y15/00 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6839 ,  C40B40/06 ,  Y02A50/57 ,  Y10S977/924 ,  C12Q2563/155 ,  C12Q2563/143 ,  C12Q2537/143 ,  C12Q2525/197 ,  C12Q2565/625 ,  C12Q2537/125 ,  C12Q2525/161

摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

公开(公告)号：US20020137046A1

公开(公告)日：2002-09-26

申请号：US09796416

申请日：2001-02-28

发明人： Hubert Koster

IPC分类号： C12Q001/68 ,  C12P019/34

CPC分类号： C12Q1/6872 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6883 ,  C12Q1/706 ,  G01N35/1067 ,  H01J49/00 ,  Y10T436/143333 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2537/113 ,  C12Q2535/113 ,  C12Q2521/325 ,  C12Q2535/131 ,  C12Q2525/107 ,  C12Q2523/107 ,  C12Q2523/319 ,  C12Q2565/625 ,  C12Q2535/125

摘要： Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Depending upon the sequence to be detected, the processes, for example, can be used to diagnose a genetic disease or a chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogen, or for determining identity or heredity.

摘要翻译： 提供了用于检测分子中特定核酸分子和序列的基于质谱的快速和高精度的方法。 根据待检测的序列，例如，该过程可用于诊断遗传疾病或染色体异常，疾病或病症的易感性或病原体的感染，或用于确定身份或遗传。

公开(公告)号：US20020127574A1

公开(公告)日：2002-09-12

申请号：US09973788

申请日：2001-10-10

申请人： Nanosphere, Inc.

发明人： Chad A. Mirkin ,  Robert L. Letsinger ,  Robert C. Mucic ,  James J. Storhoff ,  Robert Elghanian ,  Thomas A. Taton

IPC分类号： C12Q001/68

CPC分类号： B82Y30/00 ,  B01J2219/00274 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B82B1/00 ,  B82Y15/00 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6839 ,  C40B40/06 ,  Y02A50/57 ,  Y10S977/924 ,  C12Q2563/155 ,  C12Q2563/143 ,  C12Q2537/143 ,  C12Q2525/197 ,  C12Q2565/625 ,  C12Q2537/125 ,  C12Q2525/161

摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

摘要翻译： 本发明提供了检测核酸的方法。 所述方法包括使核酸与一种或多种具有与其连接的寡核苷酸的颗粒接触。 在该方法的一个实施方案中，寡核苷酸与纳米颗粒连接并具有与核酸序列的部分互补的序列。 作为纳米颗粒上的寡核苷酸与核酸的杂交的结果，导致可检测的变化（优选变色）。 本发明还提供包含颗粒的组合物和试剂盒。 本发明还提供了合成独特的纳米颗粒 - 寡核苷酸缀合物的方法，通过该方法产生的缀合物和使用缀合物的方法。 此外，本发明提供纳米材料和包含纳米颗粒的纳米结构和使用纳米颗粒的纳米加工方法。 最后，本发明提供了从其他核酸中分离选择的核酸的方法。

公开(公告)号：US06268144B1

公开(公告)日：2001-07-31

申请号：US09397766

申请日：1999-09-15

申请人： Hubert Köster

发明人： Hubert Köster

IPC分类号： C12Q168

CPC分类号： C12Q1/6872 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6883 ,  C12Q1/706 ,  G01N35/1067 ,  H01J49/00 ,  Y10T436/143333 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2537/113 ,  C12Q2535/113 ,  C12Q2521/325 ,  C12Q2535/131 ,  C12Q2525/107 ,  C12Q2523/107 ,  C12Q2523/319 ,  C12Q2565/625 ,  C12Q2535/125

摘要： Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Depending upon the sequence to be detected, the processes, for example, can be used to diagnose a genetic disease or a chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogen, or for determining identity or heredity.

公开(公告)号：US20010007746A1

公开(公告)日：2001-07-12

申请号：US09398625

申请日：1999-09-18

发明人： MARTIN A. SMITH ,  MRIDULA IYER ,  DAQING QU ,  JAMES C. DAVIS

IPC分类号： C12Q001/68 ,  C07H021/02 ,  C07H021/04

CPC分类号： C12N1/06 ,  B01L3/5023 ,  B01L3/5029 ,  B01L2300/0822 ,  B01L2300/163 ,  C12N15/1006 ,  C12Q1/6806 ,  G01N1/405 ,  Y10T436/143333 ,  C12Q2565/625

摘要： A medium for storage and subsequent analysis of a genetic material includes a support for immobilizing the genetic material thereon and allowing subsequent elution of the genetic material therefrom and a coating functionally associated with the support for enabling cellular lysis and releasing the genetic material from the lysed cells while stabilizing the immobilized released genetic material. A method of storing the genetic material and subsequently analyzing the genetic material includes the steps of immobilizing the genetic material on a support while enabling cellular lysis and release of genetic material from the lysed cells and stabilizing the immobilized released genetic material on the support. The genetic material is then eluted to generate a soluble genetic material fraction. The eluted genetic material can be analyzed.

摘要翻译： 用于遗传物质的储存和随后分析的培养基包括用于将遗传物质固定在其上的载体，并允许随后从其中洗脱遗传物质和与支持物功能相关的涂层，以使细胞裂解并从裂解的细胞释放遗传物质 同时稳定固定化释放的遗传物质。 存储遗传物质并随后分析遗传物质的方法包括以下步骤：将遗传物质固定在支持物上，同时能够从裂解的细胞中细胞裂解和释放遗传物质并稳定载体上固定的释放的遗传物质。 然后洗脱遗传物质以产生可溶性遗传物质级分。 可以分析洗脱的遗传物质。