公开(公告)号：US11697116B2

公开(公告)日：2023-07-11

申请号：US17714129

申请日：2022-04-05

申请人： ILLUMINA, INC.

发明人： Erik Williamson ,  Bryan Crane ,  Patrick Leung ,  Drew Verkade ,  Mark T. Reed

IPC分类号： B01L3/00 ,  B01L9/00 ,  G01N21/05 ,  B01L7/00

CPC分类号： B01L3/502715 ,  B01L9/527 ,  G01N21/05 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0689 ,  B01L2300/022 ,  B01L2300/041 ,  B01L2300/043 ,  B01L2300/0816 ,  B01L2300/0877 ,  G01N2021/058 ,  Y10T436/25

摘要： A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.

公开(公告)号：US20230207601A1

公开(公告)日：2023-06-29

申请号：US18175836

申请日：2023-02-28

申请人： Illumina, Inc.

发明人： Xiuyu CAI ,  Ali AGAH ,  Tracy H. FUNG ,  Dietrich DEHLINGER

IPC分类号： H01L27/146 ,  G01J1/44 ,  G01J1/02 ,  G01N21/64

CPC分类号： H01L27/14636 ,  H01L27/1462 ,  H01L27/14625 ,  H01L27/14643 ,  H01L27/14629 ,  G01J1/44 ,  H01L27/14609 ,  G01J1/0214 ,  H01L27/14601 ,  G01N21/6454 ,  G01J1/0209 ,  G01N2201/0642 ,  H01L27/14623 ,  G01N21/05

摘要： An example image sensor structure includes an image layer. The image layer includes an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with at least one light detector of the array of light detectors. A passivation stack is disposed over the device stack. The passivation stack includes a bottom surface in direct contact with a top surface of the light guides. An array of nanowells is disposed in a top layer of the passivation stack. Each nanowell is associated with a light guide of the array of light guides. A crosstalk blocking metal structure is disposed in the passivation stack. The crosstalk blocking metal structure reduces crosstalk within the passivation stack.

公开(公告)号：US20230194426A1

公开(公告)日：2023-06-22

申请号：US18154405

申请日：2023-01-13

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIGE LIMITED

发明人： Gary Skinner ,  Geraint Evans ,  Stanley Hong

IPC分类号： G01N21/64 ,  B01L3/00 ,  G01N21/05 ,  G02B21/06 ,  G02B21/16 ,  G02B21/36 ,  G02B27/42 ,  G06V10/10

CPC分类号： G01N21/6452 ,  B01L3/5085 ,  G01N21/05 ,  G02B21/06 ,  G02B21/16 ,  G02B21/365 ,  G02B27/425 ,  G06V10/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0893 ,  B01L2300/0896

摘要： Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

公开(公告)号：US20230184673A1

公开(公告)日：2023-06-15

申请号：US18106958

申请日：2023-02-07

申请人： The Automation Partnership (Cambridge) Limited

发明人： Adrian Stacey ,  Christian Grimm ,  Marek Hoehse ,  Thomas Regen ,  Angus Woodhams

IPC分类号： G01N21/359 ,  G01N21/05 ,  G01N35/00 ,  G01N35/10

CPC分类号： G01N21/359 ,  G01N21/05 ,  G01N35/00603 ,  G01N35/1095 ,  G01N2021/8416

摘要： A computer-implemented method for spectroscopic analysis of biological material is provided that includes analyzing samples of biological material from a plurality of sources, and delivering samples of biological material to at least one flow cell for spectroscopy, and determining whether the spectroscopic analysis for each sample of the plurality of samples is or is predicted to be ambiguous in that it is affected by at least two non-discriminable factors. If such a determination is made, a disambiguating step can be performed.

公开(公告)号：US20230175060A1

公开(公告)日：2023-06-08

申请号：US17939760

申请日：2022-09-07

申请人： Pacific Biosciences of California, Inc.

发明人： Adrian FEHR ,  Nathaniel Joseph MCCAFFREY ,  Stephen TURNER

IPC分类号： C12Q1/6874 ,  B01L3/00 ,  G01N21/64 ,  B82Y20/00 ,  G01N33/543 ,  G02B6/122 ,  G01N21/03 ,  G01N21/05 ,  G01N21/75 ,  G01N21/77 ,  C12Q1/6825 ,  C12Q1/6869

CPC分类号： C12Q1/6874 ,  B01L3/502707 ,  G01N21/6452 ,  G01N21/648 ,  B82Y20/00 ,  G01N21/6456 ,  G01N21/6454 ,  G01N33/54373 ,  G02B6/1226 ,  G01N21/0303 ,  G01N21/05 ,  G01N21/75 ,  G01N21/64 ,  G01N21/77 ,  B01L3/502715 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N21/03 ,  C12Q1/6869 ,  G01N21/645 ,  Y10T436/143333 ,  B01L2300/0654 ,  B01L2300/168 ,  G01N2021/0346 ,  G01N2021/757 ,  B01L2300/0663 ,  B01L2300/0816 ,  G01N2021/6434 ,  G01N2021/6441 ,  G01N2201/067 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2201/08 ,  G01N2021/7786 ,  G01N2201/068

摘要： Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

公开(公告)号：US20230160808A1

公开(公告)日：2023-05-25

申请号：US18158346

申请日：2023-01-23

申请人： LUMACYTE, INC.

发明人： Sean Hart ,  Colin Hebert ,  Christopher Field ,  Shweta Krishnan

IPC分类号： G01N15/14 ,  G01N21/05 ,  G01N21/85 ,  B01L3/00

CPC分类号： G01N15/1434 ,  G01N21/05 ,  G01N21/85 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/5027 ,  G01N15/147 ,  G01N15/1484 ,  B01L2200/10 ,  G01N2201/06113 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2300/0861 ,  B01L2400/0487 ,  B01L2400/0418 ,  B01L2400/043 ,  B01L2400/0433 ,  B01L2300/0654 ,  G01N2015/1445 ,  B01L2300/0874 ,  G01N2015/1006

摘要： A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging. A laser may also suspend cells or particles in this channel during analysis which prevents them from settling.

公开(公告)号：US11656165B2

公开(公告)日：2023-05-23

申请号：US17041511

申请日：2019-03-27

申请人： Max Planck Society ,  Otto-von-Guericke University Magdeburg

发明人： Torsten Schroeder ,  Franz Detlef ,  Erik Temmel ,  Steve Haltenhof ,  Andreas Wuensch ,  René Ebling

IPC分类号： G01N15/00 ,  G01N15/02 ,  G01N21/05

CPC分类号： G01N15/0227 ,  G01N21/05

摘要： The invention relates to a measuring cell for analysing a fluid flow, wherein the measuring cell has a channel for conducting the fluid flow, which channel is divided into different sections and the cross section thereof is perpendicular to a longitudinal axis. The invention also relates to a measuring device comprising such a measuring cell.

公开(公告)号：US20190212256A1

公开(公告)日：2019-07-11

申请号：US16333146

申请日：2017-09-07

申请人： BASF COATINGS GMBH

发明人： Andreas Joch ,  Michael Schaefer ,  Carlos Arthur Leaes Peixoto ,  Juergen Ettmueller ,  Stefan Ziegler ,  Pieter Moonen

IPC分类号： G01N21/25 ,  G01J3/52 ,  G01N33/32 ,  G01J3/50 ,  G01N21/53

CPC分类号： G01N21/251 ,  G01J3/501 ,  G01J3/524 ,  G01N1/2035 ,  G01N21/0303 ,  G01N21/05 ,  G01N21/255 ,  G01N21/4785 ,  G01N21/53 ,  G01N21/532 ,  G01N21/534 ,  G01N33/32 ,  G01N2021/036 ,  G01N2021/1738 ,  G01N2021/4707 ,  G01N2021/4709 ,  G01N2021/4711 ,  G01N2201/06146 ,  G01N2201/0668 ,  G01N2201/08

摘要： Described herein is a sensor for a virtually simultaneous measurement of transmission and/or forward scattering and/or remission and for a simultaneous measurement of the transmission and forward scattering or the transmission and remission of a liquid sample. Further described herein is a method for a virtually simultaneous measurement of transmission and/or forward scattering and/or remission and for a simultaneous measurement of the transmission and forward scattering or the transmission and remission of a liquid sample using a sensor according to the invention. Further described herein is a method for using the sensor according to the invention in order to determine the color properties of painting agents such as lacquers, dyes, pastes, and pigments or dilutions thereof.

公开(公告)号：US20190198553A1

公开(公告)日：2019-06-27

申请号：US16208862

申请日：2018-12-04

申请人： Illumina, Inc.

发明人： Xiuyu CAI ,  Ali AGAH ,  Tracy H. FUNG ,  Dietrich DEHLINGER

IPC分类号： H01L27/146

CPC分类号： H01L27/14636 ,  G01J1/0209 ,  G01J1/0214 ,  G01J1/44 ,  G01N21/05 ,  G01N21/6454 ,  G01N2201/0642 ,  H01L27/14601 ,  H01L27/14609 ,  H01L27/1462 ,  H01L27/14623 ,  H01L27/14625 ,  H01L27/14629 ,  H01L27/14643

摘要： An example image sensor structure includes an image layer. The image layer includes an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with at least one light detector of the array of light detectors. A passivation stack is disposed over the device stack. The passivation stack includes a bottom surface in direct contact with a top surface of the light guides. An array of nanowells is disposed in a top layer of the passivation stack. Each nanowell is associated with a light guide of the array of light guides. A crosstalk blocking metal structure is disposed in the passivation stack. The crosstalk blocking metal structure reduces crosstalk within the passivation stack.

公开(公告)号：US20190185909A1

公开(公告)日：2019-06-20

申请号：US16053389

申请日：2018-08-02

申请人： Coyote Bioscience Co., Ltd.

发明人： Xiang LI ,  Chen Li ,  Kun Yang

IPC分类号： C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q1/70 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0877 ,  B01L2300/14 ,  B01L2300/1805 ,  B01L2400/0487 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q1/703 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/035

摘要： The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples.