公开(公告)号：US20110306110A1

公开(公告)日：2011-12-15

申请号：US13133624

申请日：2009-12-14

申请人： Shoji Takeuchi ,  Yukiko Matsunaga ,  Yuya Morimoto

发明人： Shoji Takeuchi ,  Yukiko Matsunaga ,  Yuya Morimoto

IPC分类号： C12N5/071 ,  C12N11/10 ,  C12N5/0775 ,  C12N11/02

CPC分类号： C12N5/0691 ,  A61L27/3839 ,  C12N5/0075 ,  C12N5/0671 ,  C12N2502/1323 ,  C12N2513/00 ,  C12N2531/00 ,  C12N2533/54

摘要： A method for preparing a biomaterial comprising a gel layer which forms a core region, and cells (cover cells) which cover around the gel layer, said method comprising the steps of:(a) bringing a biocompatible oil or a vegetable oil or a mixture thereof with a mineral oil into contact with a solution containing a gel-forming component to form monodisperse droplets;(b) inducing gelation of the monodisperse droplets to give gel beads; and(c) seeding the cover cells over the surface of the gel beads.

摘要翻译： 一种制备生物材料的方法，包括形成核心区域的凝胶层和覆盖在凝胶层周围的细胞（覆盖细胞），所述方法包括以下步骤：（a）使生物相容性油或植物油或混合物 与矿物油与含有凝胶形成组分的溶液接触以形成单分散液滴; （b）引起单分散液滴的凝胶化，得到凝胶珠; 和（c）将覆盖细胞接合在凝胶珠的表面上。

公开(公告)号：US20110183398A1

公开(公告)日：2011-07-28

申请号：US12811142

申请日：2008-12-23

申请人： Sridhar V. Dasaratha ,  G.M. Bommarito ,  Tushar A. Kshirsagar

发明人： Sridhar V. Dasaratha ,  G.M. Bommarito ,  Tushar A. Kshirsagar

IPC分类号： C12N11/10 ,  C12N1/20 ,  C12M1/00

CPC分类号： G01N33/54353 ,  G01N33/54333 ,  G01N33/548 ,  G01N33/56911

摘要： The invention relates to compositions, methods, devices, and kits for non-specifically isolating bacterial cells. The compositions comprise a solid support which has a surface comprising a combination of a carbohydrate and a biotin-binding protein, wherein the protein is covalently bonded to the carbohydrate, and wherein the protein is linked to the solid support via the carbohydrate; and at least one of 1) a plurality of bacterial cells non-specifically bound to the combination of the carbohydrate and the protein or 2) an amphiphilic glycoside of a steroid or triterpene. The methods, devices, and kits include at least one of these compositions.

摘要翻译： 本发明涉及用于非特异性分离细菌细胞的组合物，方法，装置和试剂盒。 所述组合物包含固体支持物，其具有包含碳水化合物和生物素结合蛋白的组合的表面，其中所述蛋白质与碳水化合物共价键合，并且其中所述蛋白质通过所述碳水化合物与所述固体支持物连接; 和1）与碳水化合物和蛋白质的组合非特异性结合的多个细菌细胞中的至少一个或2）类固醇或三萜烯的两亲糖苷。 方法，装置和试剂盒包括这些组合物中的至少一种。

公开(公告)号：US07935334B2

公开(公告)日：2011-05-03

申请号：US11493859

申请日：2006-07-26

申请人： Jhy-Jhu Lin

发明人： Jhy-Jhu Lin

IPC分类号： A61K45/00 ,  A01N25/34 ,  A01N63/00 ,  C12N1/20 ,  C12N11/02 ,  C12N11/10

CPC分类号： A61K35/741 ,  A23K10/18 ,  A23K20/163 ,  A23K40/30 ,  A23K50/40 ,  A23K50/75 ,  A61K35/744 ,  A61K36/06 ,  A61K39/39 ,  A61K2039/55594 ,  C12N11/10 ,  Y10S435/822

摘要： An exemplary embodiment providing one or more improvements includes feeding animals with probiotic microbes encapsulated in a mixture of xanthan gum and chitosan, or in gelatin, specifically Pediococcus acidilactici and Saccharomyces boulardii. Such encapsulation protects the viability of the probiotic microbes against unfavorable temperatures. An exemplary embodiment providing one or more improvements includes methods of using viable probiotics in therapy of birds and mammals infected with infectious diseases. Probiotics acted as adjuvants in stimulating antibody reaction and stimulated a cellular immunity response. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.

摘要翻译： 提供一个或多个改进的示例性实施方案包括将包含在黄原胶和壳聚糖的混合物中的益生菌微生物饲喂动物，或者在明胶中，特别是酸性乳酸球菌和布氏酵母（Saccharomyces boulardii）。 这种包封可以保护益生菌微生物对不利温度的生存力。 提供一个或多个改进的示例性实施方案包括在感染感染性疾病的鸟类和哺乳动物的治疗中使用活的益生菌的方法。 益生菌作为刺激抗体反应的佐剂，刺激细胞免疫反应。 特别地，益生菌显示从粪便样品中减少活卵母细胞的数量，刺激抗体产生，并刺激感染艾美球虫的鸡脾细胞的增殖。 此外，益生菌被证明可以缓解狗中细小病毒感染的症状。

公开(公告)号：US20110076737A1

公开(公告)日：2011-03-31

申请号：US12866748

申请日：2009-06-11

申请人： Magdy Mahmoud Mustafa Elnashar

发明人： Magdy Mahmoud Mustafa Elnashar

IPC分类号： C12N11/10 ,  C07K17/12

CPC分类号： C07K17/10 ,  C12N11/04 ,  C12N11/06 ,  C12N11/10

摘要： This patent involves improvement of the thermal stability of natural polymers, “hydrogels”, to be used as supports for covalent immobilization of enzymes. The presence of the new functionality has been proved by FTIR. In brief, the new polymeric carrier has the main following advantages: • Natural polymer & friendly to the environment • Highly hydrophilic • Containing active groups for covalent immobilization of enzymes& antibodies Thermally stable (35-95° C.) Porous The modified carrier could be used for immobilization of enzymes on the industrial scale. The immobilized enzymes could be reused for tens of times, which will reduce the cost of the enzymes and their products.

摘要翻译： 该专利涉及天然聚合物“水凝​​胶”的热稳定性的改进，用作酶共价固定的载体。 新功能的出现已被FTIR证明。 简而言之，新的聚合物载体具有以下主要优点：•天然聚合物，对环境友好•高亲水性•含酶和抗体共价固定的活性基团热稳定（35-95°C）多孔改性载体可以是 用于在工业规模上固定酶。 固定化酶可以重复使用数十次，这将降低酶及其产物的成本。

公开(公告)号：US07723086B2

公开(公告)日：2010-05-25

申请号：US10869002

申请日：2004-06-16

申请人： Hanry Yu ,  Hai Ting Ho ,  Jing Zhang

发明人： Hanry Yu ,  Hai Ting Ho ,  Jing Zhang

IPC分类号： C12N11/04 ,  C12N11/02 ,  C12N11/10 ,  B01J13/02

CPC分类号： C12N11/04 ,  B01J13/10 ,  Y10S530/817

摘要： An apparatus for encapsulating cells is disclosed. According to one embodiment, the apparatus includes an indirect-pumping dispenser for dispensing a cell suspension into an encapsulation solution through an outlet of the dispenser. The apparatus also includes a dipping mechanism that is attachable to the outlet of the dispenser. The dipping mechanism is adapted to dip the dispenser outlet in the encapsulation solution to allow the cell suspension dispensed thereat to come into contact with the encapsulation solution.

摘要翻译： 公开了一种封装电池的装置。 根据一个实施例，该装置包括间接泵送分配器，用于通过分配器的出口将细胞悬浮液分配到包封溶液中。 该装置还包括可附接到分配器的出口的浸渍机构。 浸渍机构适于将分配器出口浸入包封溶液中以允许在其中分配的细胞悬浮液与封装溶液接触。

公开(公告)号：US20100093053A1

公开(公告)日：2010-04-15

申请号：US12497591

申请日：2009-07-03

申请人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

发明人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

IPC分类号： C12N11/14 ,  C12N5/02 ,  C12N11/00 ,  C12N11/12 ,  C12N11/10 ,  C12N11/02

CPC分类号： C12N5/0606 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0647

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

摘要翻译： 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。

公开(公告)号：US20090325263A1

公开(公告)日：2009-12-31

申请号：US12439274

申请日：2007-09-13

申请人： Reddy Ponaka ,  Joseph W. Farchaus ,  Michael D. Pierce

发明人： Reddy Ponaka ,  Joseph W. Farchaus ,  Michael D. Pierce

IPC分类号： C12N11/10

CPC分类号： C12N1/04 ,  B01L3/50851 ,  C12N9/96 ,  C12Q1/6806 ,  C12Q2527/137

摘要： The invention related to a method of making a dried reagent preparation, comprising the steps of: providing an aqueous solution of at least one buffered biological reagent; mixing a glass forming filler material with the buffered reagent solution to form a mixture wherein the concentration of the filler material is sufficient to facilitate formation of a glassy, porous composition; dispensing the mixture in the form of substantially uniform droplets into wells of a multi-well container, wherein a single droplet is dispensed into each well; drying the droplets in the container to form the reagent preparation. The reagent preparation is water soluble and has a Tg sufficient for room temperature stability.

摘要翻译： 本发明涉及一种制备干燥试剂制备方法，包括以下步骤：提供至少一种缓冲生物试剂的水溶液; 将玻璃形成填充材料与缓冲的试剂溶液混合以形成混合物，其中填充材料的浓度足以促进形成玻璃质多孔组合物; 将混合物以基本均匀的液滴的形式分配到多孔容器的孔中，其中单个液滴分配到每个孔中; 干燥容器中的液滴以形成试剂制剂。 试剂制剂是水溶性的，具有足以使室温稳定的Tg。

公开(公告)号：US20090275637A1

公开(公告)日：2009-11-05

申请号：US12366562

申请日：2009-02-05

申请人： Brent Townshend ,  Michel Tremblay

发明人： Brent Townshend ,  Michel Tremblay

IPC分类号： A61K31/713 ,  C12N15/55 ,  C12N11/10 ,  C40B40/08 ,  C12N9/99

CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12Y301/03048

摘要： The present invention provides compositions and methods for binding and/or modulating enzymatic activity of human protein tyrosine phosphatases such as PTP1B. Additionally, the invention provides methods of identifying and using such nucleic acid ligands.

摘要翻译： 本发明提供了用于结合和/或调节人蛋白质酪氨酸磷酸酶如PTP1B的酶活性的组合物和方法。 另外，本发明提供鉴定和使用这种核酸配体的方法。

公开(公告)号：US07572456B2

公开(公告)日：2009-08-11

申请号：US11226886

申请日：2005-09-13

申请人： Leslie S. Johnson ,  Ling Huang

发明人： Leslie S. Johnson ,  Ling Huang

IPC分类号： A61K39/00 ,  A61K39/12 ,  A61Q5/00 ,  C12P7/24 ,  C12P7/52 ,  C12N5/16 ,  C12N11/10

CPC分类号： C07K16/1081 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/567 ,  G01N33/56983 ,  G01N2333/18 ,  G01N2469/20 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/394 ,  Y02A50/396 ,  Y02A50/53 ,  Y02A50/60

摘要： The present invention relates to compositions comprising humanized antibodies or fragments thereof that immunospecifically bind to one or more antigens of a flavivirus, particularly of West Nile Virus (WNV) and methods for preventing, treating or ameliorating symptoms associated with a flavivirus, particularly of West Nile Virus (WNV) infection utilizing said compositions. In particular, the present invention relates to methods for preventing, treating or ameliorating symptoms associated with WNV infection, said methods comprising administering to a human subject an effective amount of one or more humanized antibodies or fragments thereof that immunospecifically bind to a WNV antigen. The present invention also relates to detectable or diagnostic compositions comprising humanized antibodies or fragments thereof that immunospecifically bind to a WNV antigen and methods for detecting or diagnosing WNV infection utilizing said compositions.

摘要翻译： 本发明涉及包含人源化抗体或其片段的组合物，其免疫特异性结合黄病毒，特别是西尼罗病毒（WNV）的一种或多种抗原，以及用于预防，治疗或改善与黄病毒特别是西尼罗病毒有关的症状的方法 利用所述组合物的病毒（WNV）感染。 特别地，本发明涉及用于预防，治疗或改善与WNV感染相关的症状的方法，所述方法包括向人类受试者施用有效量的一种或多种免疫特异性结合WNV抗原的人源化抗体或其片段。 本发明还涉及包含免疫特异性结合WNV抗原的人源化抗体或其片段的可检测或诊断组合物，以及利用所述组合物检测或诊断WNV感染的方法。

公开(公告)号：USRE40555E1

公开(公告)日：2008-10-28

申请号：US10979527

申请日：2004-11-01

申请人： Kanti Jain ,  Albert L. Rubin ,  Barry Smith

发明人： Kanti Jain ,  Albert L. Rubin ,  Barry Smith

IPC分类号： A61K35/12 ,  C12N11/02 ,  C12N11/10 ,  C12N5/00 ,  C12N5/08

CPC分类号： A61K9/1652 ,  A61K9/1658 ,  A61K9/5036 ,  A61K35/39 ,  A61K38/00 ,  A61K2035/126 ,  C12N5/0012 ,  C12N5/0677 ,  C12N11/04 ,  C12N2533/54 ,  C12N2533/76

摘要： Biological agents such as secretory cells are encapsulated in a hydrophilic gel made of agarose or collagen-agarose and gelatin sponge-agarose combinations. In a preferred embodiment, semi-solid beads are formed from a suspension containing collagen, agarose and secretory cells such as pancreatic islets, the collagen is polymerized to form solid, agarose-collagen beads and the solid beads are coated with agarose. Coating is preferably by rolling the solid beads in about 5-10% agarose, contacting the rolled beads with mineral oil and washing oil from the beads. Beads containing secretory cells can be transplanted into a mammal to treat a condition caused by impaired secretory cell function.

摘要翻译： 生物制剂如分泌细胞被包封在由琼脂糖或胶原 - 琼脂糖和明胶海绵 - 琼脂糖组合制成的亲水凝胶中。 在优选的实施方案中，半固体珠粒由含有胶原蛋白，琼脂糖和分泌细胞例如胰岛的悬浮液形成，胶原聚合形成固体，琼脂糖胶原珠粒，固体珠粒用琼脂糖涂覆。 涂覆优选通过在约5-10％的琼脂糖中滚动固体珠粒，使滚珠与矿物油接触并从珠粒洗涤油。 含有分泌细胞的珠可以移植到哺乳动物中以治疗由分泌细胞功能受损引起的病症。