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    METABOLIC ASSAY FOR BACTERIAL GROWTH AND GRAM TYPING
    32.
    发明申请
    METABOLIC ASSAY FOR BACTERIAL GROWTH AND GRAM TYPING 审中-公开

    公开(公告)号：US20190300927A1

    公开(公告)日：2019-10-03

    申请号：US16366244

    申请日：2019-03-27

    Applicant: SELUX DIAGNOSTICS, INC.

    Inventor： Benjamin Spears ,  Eric Stern ,  Kelly Flentie

    IPC: C12Q1/20 ,  C12Q1/08

    Abstract: The use of metabolic probes is well-established for determining cell viability and assessing drug cytotoxicity. Resazurin-based formulations, in particular, have found utility for determining susceptibilities of microorganisms to antimicrobials, specifically through their use in antibiotic susceptibility testing (AST). There is a strong need currently to shorten AST durations, thus resazurin formulations that produce signals earlier are advantageous. This need results from the slow state of clinical microbiology testing, which may leave patients exposed to unnecessary or ineffective broad-spectrum agents for prolonged periods of time. Another slow microbiology test is Gram staining, still most often performed manually in sequential steps. Speeding Gram typing, preferably with an automated platform, would also speed time-to-results and decrease manual workloads on medical technologists in clinical microbiology laboratories.

    Catalytic strands of minimal hammerhead ribozymes and methods of using the same
    33.
    发明授权
    Catalytic strands of minimal hammerhead ribozymes and methods of using the same 有权

    公开(公告)号：US10301626B2

    公开(公告)日：2019-05-28

    申请号：US15554660

    申请日：2016-03-01

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Sara Marie O'Rourke ,  William Gregory Scott

    IPC: C12N15/113 ,  A61K31/70 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/08 ,  C12N15/11

    Abstract: Provided are minimal hammerhead ribozymes and catalytic strands thereof. Aspects of the present disclosure include a catalytic strand of a minimal hammerhead ribozyme, the catalytic strand including a catalytic core region, a stem I-forming region, a stem II region, and a stem III-forming region. The catalytic strand hybridizes to a target strand via the stem I-forming region and the stem III-forming region. A nucleotide (e.g., an adenine, cytosine, or a natural or non-natural nucleotide base having hydrogen bond donor and acceptor functionalities at positions analogous to those of adenine or cytosine) present in a stem II loop base pairs with a nucleotide (e.g., uracil or cytosine) at position 1.7 of the target strand. Also provided are compositions that include the catalytic strands, and methods of using the catalytic strands, e.g., in a variety of different applications, as well as kits that find use in practicing embodiments of the methods.

    TEST DEVICE
    35.
    发明申请
    TEST DEVICE 审中-公开

    公开(公告)号：US20180010084A1

    公开(公告)日：2018-01-11

    申请号：US15544302

    申请日：2016-01-22

    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

    Inventor： Chihiro UEMATSU ,  Muneo MAESHIMA

    IPC: C12M1/34 ,  C12M1/32 ,  C12Q1/08 ,  G06T7/00 ,  G02B21/36 ,  G02B21/26

    CPC classification number: C12M41/36 ,  C12M1/34 ,  C12M23/12 ,  C12M41/46 ,  C12Q1/04 ,  C12Q1/08 ,  G02B21/26 ,  G02B21/367 ,  G02B21/368 ,  G06T1/00 ,  G06T7/0016 ,  G06T2207/10056

    Abstract: The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG. 12).

    Modular compositing-multiple lot screening protocols for detection of pathogens, microbial contaminants and/or constituents
    37.
    发明授权
    Modular compositing-multiple lot screening protocols for detection of pathogens, microbial contaminants and/or constituents 有权
    用于检测病原体，微生物污染物和/或成分的模块化复合多批筛选方案

    公开(公告)号：US08389233B2

    公开(公告)日：2013-03-05

    申请号：US12360796

    申请日：2009-01-27

    Applicant: Mansour Samadpour

    Inventor： Mansour Samadpour

    IPC: C12Q1/08 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12Q1/20 ,  G01N33/53 ,  G01N33/573 ,  C12Q1/14 ,  C12Q1/18 ,  C12Q1/22 ,  C12M1/00

    CPC classification number: C12Q1/10 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/28 ,  Y10S435/849

    Abstract: Provided are methods for sampling, testing and validating test lots, comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the portions to provide a corresponding set of test lot samples; enriching the test lot samples; removing portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample, and individual testing of the enriched test lot samples, using at least one suitable detection assay for a target microbe or organism, wherein when such testing is negative all test lots are validated, and wherein when such testing is positive with respect to the modular composite sample, or with respect to an individual enriched test lot sample, individual test lots may nonetheless yet be validated by further testing of a portion of respective initially-negative enriched test lot samples and obtaining negative results.

    Abstract translation: 提供了用于对测试批进行采样，测试和验证的方法，包括：从多个测试批中的每一个组装多个产品部分并组合该部分以提供相应的一组测试批样本; 丰富测试批样本; 去除每个富集样品的部分，并组合去除的部分以提供模块化复合样品; 使用至少一种适用于目标微生物或生物体的检测试验，对模块化复合样品进行测试，并对富集测试批样本进行单独测试，其中当这种测试为阴性时，所有测试批次都被验证，并且其中当这种测试是 对于模块化复合样品，或相对于个体富集的测试批样品，单个测试批次仍然可以通过进一步测试相应的初始负极富集的测试批样本的一部分并获得阴性结果来验证。

    Cell sample preparation method and apparatus
    38.
    发明授权
    Cell sample preparation method and apparatus 有权
    细胞样品制备方法和装置

    公开(公告)号：US08119363B2

    公开(公告)日：2012-02-21

    申请号：US12114155

    申请日：2008-05-02

    Applicant: Ernest A. Knesel ,  Bradley W. Knesel ,  Joel C. Dry ,  Kevin J. Rackers ,  Robert M. Hicks, Jr. ,  Benjamin A. Perrot

    Inventor： Ernest A. Knesel ,  Bradley W. Knesel ,  Joel C. Dry ,  Kevin J. Rackers ,  Robert M. Hicks, Jr. ,  Benjamin A. Perrot

    IPC: C12Q1/08

    CPC classification number: G01N1/2813

    Abstract: An apparatus and method for automatic thin-layer cell sample slide preparation without requiring human intervention are disclosed. According to one embodiment, a cell sample is measured to derive a cell sample measurement. An estimation of total cellularity of the cell sample is determined based upon the cell sample measurement. A differential volume of diluent is dispensed to the cell sample based upon the estimation of total cellularity of the cell sample to form a cell suspension. In one embodiment, a differential volume of the cell suspension and a differential volume of cell adherent may be combined based upon the estimation of total cellularity of the cell sample to form a cell mixture. A differential volume of the cell suspension or cell mixture (if an adherent is mixed with the cell suspension) is dispensed onto a surface of a sample slide.

    Abstract translation: 公开了一种用于自动薄层细胞样品载玻片制备而不需要人为干预的装置和方法。 根据一个实施例，测量细胞样品以导出细胞样品测量。 基于细胞样品测量确定细胞样品的总细胞数的估计。 基于细胞样品的总细胞数的估计，将稀释液的不同体积分配到细胞样品中以形成细胞悬浮液。 在一个实施方案中，可以基于细胞样品的总细胞数的估计来组合细胞悬浮液的差异体积和细胞粘附的差异体积以形成细胞混合物。 将细胞悬浮液或细胞混合物（如果粘附剂与细胞悬浮液混合）的差异体积分配到样品载玻片的表面上。

    Tissue-specific and target RNA-specific ribozymes
    39.
    发明授权
    Tissue-specific and target RNA-specific ribozymes 失效
    组织特异性和靶RNA特异性核酶

    公开(公告)号：US07732197B2

    公开(公告)日：2010-06-08

    申请号：US10082973

    申请日：2002-02-26

    Applicant: James S. Norris ,  Gary A. Clawson ,  Michael G. Schmidt ,  Brian Hoel ,  Wei-Hua Pan ,  Joseph W. Dolan

    Inventor： James S. Norris ,  Gary A. Clawson ,  Michael G. Schmidt ,  Brian Hoel ,  Wei-Hua Pan ,  Joseph W. Dolan

    IPC: C07H21/02 ,  C07H21/04 ,  A61K31/70 ,  C12Q1/08 ,  C12N5/00 ,  C12N15/00

    CPC classification number: C12N15/113 ,  A61K38/00 ,  C12N2310/111 ,  C12N2310/121 ,  C12N2310/127 ,  C12N2799/021

    Abstract: The present invention relates to multi-ribozymes and their use to target RNA in a tissue-specific, target RNA-specific, or pathogen-specific manner for the treatment of cancers, proliferative disease, and bacterial, parasitic and viral infections. More specifically, the present invention relates to the use of virions and viral vectors to package and deliver DNA encoding the multi-ribozymes to a host. The present invention relates to the use of liposomes and lipid-DNA complexes to deliver DNA encoding ribozymes to a host. Most specifically, the invention relates to the use of target specific virions to package and deliver DNA comprising a target specific promoter and encoding a ribozyme(s) directed to the target organism nucleic acids. The present invention further relates to a novel vectors encoding a multi-ribozyme structure with enhanced 5′ and/or 3′ autocatalytically cleaving ribozymes. The invention further relates to nucleotides encoding a multi-ribozyme comprising one or more ribozyme cassettes which contain one or more trans-acting ribozymes and one or more autocatalytically cleaving ribozyme sequences.

    Abstract translation: 本发明涉及多核酶及其用于以组织特异性，靶RNA特异性或病原体特异性方式靶向用于治疗癌症，增殖性疾病和细菌，寄生虫和病毒感染的RNA的用途。 更具体地，本发明涉及病毒粒子和病毒载体用于将编码多核酶的DNA包装并递送至宿主的用途。 本发明涉及脂质体和脂质DNA复合物将编码核酶的DNA递送至宿主的用途。 最具体地，本发明涉及靶特异性病毒体用于包装和递送包含靶特异性启动子并编码针对靶生物核酸的核酶的DNA的用途。 本发明还涉及编码具有增强的5'和/或3'自动催化裂解核酶的多核酶结构的新载体。 本发明还涉及编码多核酶的核苷酸，其包含一个或多个含有一个或多个反式作用核酶和一个或多个自动催化裂解核酶序列的核酶盒。

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