公开(公告)号：US20050287541A1

公开(公告)日：2005-12-29

申请号：US10947249

申请日：2004-09-23

申请人： Akira Nakagawara ,  Miki Ohira ,  Shin Ishii ,  Takeshi Goto ,  Hiroyuki Kubo ,  Takahiro Hirata ,  Yasuko Yoshida ,  Saichi Yamada

发明人： Akira Nakagawara ,  Miki Ohira ,  Shin Ishii ,  Takeshi Goto ,  Hiroyuki Kubo ,  Takahiro Hirata ,  Yasuko Yoshida ,  Saichi Yamada

IPC分类号： C12M1/34 ,  C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/118

摘要： A microarray for predicting the prognosis of neuroblastoma, wherein the microarray has 25 to 45 probes related to good prognosis, which are hybridized to a gene transcript whose expression is increased in a good prognosis patient with neuroblastoma and are selected from 96 polynucleotides consisting of the nucleotide sequences of Seq. ID No. 1 to 96 or their partial continuous sequences or their complementary strands, and 25 to 45 probes related to poor prognosis, which are hybridized to a gene transcript whose expression is increased in a poor prognosis patient with neuroblastoma and are selected from 104 polynucleotides consisting of the nucleotide sequences of Seq. ID No. 97 to 200 or their partial continuous sequences or their complementary strands.

摘要翻译： 一种用于预测神经母细胞瘤的预后的微阵列，其中所述微阵列具有与良好预后相关的25至45个探针，其与在具有神经母细胞瘤的良好预后患者中表达增加的基因转录物杂交，并且选自96个由核苷酸 序列Seq。 ID No.1至96或其部分连续序列或其互补链，以及与不良预后相关的25至45个探针，其与在具有神经母细胞瘤的不良预后患者中表达增加的基因转录物杂交并选自104个多核苷酸 由Seq的核苷酸序列组成。 ID号97至200或其部分连续序列或其互补链。

公开(公告)号：US20050282177A1

公开(公告)日：2005-12-22

申请号：US10946068

申请日：2004-09-22

申请人： Masao Seto ,  Hiroyuki Tagawa ,  Yasuko Yoshida ,  Shigeki Kira

发明人： Masao Seto ,  Hiroyuki Tagawa ,  Yasuko Yoshida ,  Shigeki Kira

IPC分类号： C12M1/34 ,  C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/178

摘要： A method for determining the prognosis of a CD5+DLBCL patient and a CD5−DLBCL patient is provided. It is determined that, in the chromosomal DNA from a patient with lymphoma, (1) the prognosis of the CD5+DLBCL patient with amplification of 13q21.1-q31.3 region is poor; (2) the prognosis of the CD5+DLBCL patient with deletion of 1p36.21-p36.13 region is poor; and (3) the prognosis of the CD5−DLBCL patient with amplification of 5p15.33-p14.2 region is good.

摘要翻译： 提供了一种确定CD5 + DLBCL患者和CD5-DLBCL患者预后的方法。 确定在淋巴瘤患者的染色体DNA中，（1）扩增13q21.1-q31.3区域的CD5 + DLBCL患者的预后差; （2）缺失1p36.21-p36.13区域的CD5 + DLBCL患者的预后差; 和（3）扩增5p15.33-p14.2区域的CD5-DLBCL患者的预后良好。

公开(公告)号：US06764945B2

公开(公告)日：2004-07-20

申请号：US09822318

申请日：2001-04-02

申请人： Hiroshi Ashihara ,  Tatsuyuki Saito ,  Uitsu Tanaka ,  Hidenori Suzuki ,  Hideaki Tsugane ,  Yasuko Yoshida ,  Ken Okutani

发明人： Hiroshi Ashihara ,  Tatsuyuki Saito ,  Uitsu Tanaka ,  Hidenori Suzuki ,  Hideaki Tsugane ,  Yasuko Yoshida ,  Ken Okutani

IPC分类号： H01L214763

CPC分类号： H01L21/2855 ,  H01L21/28556 ,  H01L21/32051 ,  H01L21/76801 ,  H01L21/76804 ,  H01L21/76805 ,  H01L21/76814 ,  H01L21/76834 ,  H01L21/76837 ,  H01L21/76843 ,  H01L21/76846 ,  H01L21/7685 ,  H01L21/76855 ,  H01L21/76877 ,  H01L2221/1078 ,  H01L2924/0002 ,  H01L2924/00

摘要： In order to form a good contact between metallizations and improve the reliability and product yield of a semiconductor integrated circuit device, a plug is formed in a contact hole by depositing a first sputter film inside of the contact hole by traditional sputtering, depositing a second sputter film over the first sputter film by long throw sputtering, depositing a W film over the second sputtering film by CVD and removing the first and second sputter films and the W film from the outside of the contact hole. The barrier properties can be improved by constituting a barrier film from the first sputter film and second sputter film which are different in directivity.

摘要翻译： 为了在金属化之间形成良好的接触并提高半导体集成电路器件的可靠性和产品产量，通过传统溅射在接触孔内部沉积第一溅射膜，在接触孔中形成插塞，沉积第二溅射 通过长溅射溅射第一溅射膜上的膜，通过CVD在第二溅射膜上沉积W膜，并从接触孔的外部除去第一和第二溅射膜和W膜。 可以通过从方向性不同的第一溅射膜和第二溅射膜构成阻挡膜来改善阻挡性。

公开(公告)号：US20110229980A1

公开(公告)日：2011-09-22

申请号：US12982931

申请日：2010-12-31

申请人： Hiroyuki ASANUMA ,  Xingguo Liang ,  Hiromu Kashida ,  Yasuko Yoshida ,  Tomokazu Takase ,  Kousuke Niwa

发明人： Hiroyuki ASANUMA ,  Xingguo Liang ,  Hiromu Kashida ,  Yasuko Yoshida ,  Tomokazu Takase ,  Kousuke Niwa

IPC分类号： G01N33/53 ,  C07H21/00

CPC分类号： C12Q1/6816 ,  C12Q2525/301 ,  C12Q2565/501

摘要： The present teaching provides a fluorescent oligonucleotide probe having a high degree of design flexibility and wide applicability, as well as the use thereof. This is an oligonucleotide probe capable of forming a stem and loop, comprising at least one fluorophore located between adjacent nucleotides in the stem and is linked to a unit represented by Formula (1) and at least one quencher located at a site capable of pairing up with the at least one fluorophore located between the adjacent nucleotides in the stem and is linked to a unit represented by Formula (2). (In the formulae, X represents the fluorophore, Y represents the quencher, R1 represents an optionally substituted C2 or C3 alkylene chain, R2 represents an optionally substituted C0-2 alkylene chain, and Z represents a direct bond or linker.)

摘要翻译： 本教导提供具有高设计灵活性和广泛适用性的荧光寡核苷酸探针及其用途。 这是能够形成茎和环的寡核苷酸探针，其包含位于茎中相邻核苷酸之间的至少一个荧光团，并连接到由式（1）表示的单元和位于能够配对的位点处的至少一个猝灭剂 其中所述至少一个荧光团位于茎中相邻核苷酸之间，并与由式（2）表示的单元连接。 （式中，X表示荧光团，Y表示猝灭剂，R 1表示任选取代的C 2或C 3亚烷基链，R 2表示任选取代的C 0-2亚烷基链，Z表示直接键或连接基。

公开(公告)号：US20090042734A1

公开(公告)日：2009-02-12

申请号：US11886676

申请日：2006-03-27

申请人： Yasuko Yoshida ,  Kazunari Yamada ,  Tomokazu Takase ,  Akinobu Oribe

发明人： Yasuko Yoshida ,  Kazunari Yamada ,  Tomokazu Takase ,  Akinobu Oribe

IPC分类号： C40B40/10 ,  C40B30/04 ,  C40B60/14 ,  C40B40/14 ,  C40B50/18 ,  C40B40/00

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00542 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00675 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0887 ,  C12Q1/6837

摘要： An object of the present invention is to provide a probe array having partitioned array regions with uniform surface chemical properties. The probe array of the present invention includes a substrate having a plurality of partitioned array regions where many probes are immobilized and a separator attached to the substrate and including partitions partitioning the array regions. The above object can be achieved by attaching the separator including the partitions capable of partitioning instead of forming hydrophobic regions on a surface of the substrate by printing or chemical treatment.

摘要翻译： 本发明的目的是提供一种具有均匀表面化学性质的分隔阵列区的探针阵列。 本发明的探针阵列包括具有多个分隔阵列区域的基底，其中许多探针被固定，并且分离器附着到基底上，并且包括分隔阵列区域的分隔物。 上述目的可以通过印刷或化学处理将包括能够分隔而不是通过印刷或化学处理形成疏水性区域的分隔体附接到基板的表面来实现。

公开(公告)号：US20080287313A1

公开(公告)日：2008-11-20

申请号：US12219126

申请日：2008-07-16

申请人： Yasuko Yoshida ,  Toshikazu Hirota ,  Yukihisa Takeuchi

发明人： Yasuko Yoshida ,  Toshikazu Hirota ,  Yukihisa Takeuchi

IPC分类号： C40B30/04

CPC分类号： G01N33/54373 ,  B01J2219/00605 ,  B01J2219/00637 ,  B01J2219/00704 ,  G01N33/5438

摘要： A novel chip capable of reducing a reaction period, applying wide-ranging target substance, preventing a mismatch binding efficiently and enabling a highly accurate detection is provided. Thus, an inventive reactive chip has the capture probe (60) fixed on each of three or more vibration areas (50) arranged on the support (30), the capture probes being able to binding to a target substance, wherein each vibration area has the vibration-generating part (40) having the first electrode (11) and the second electrode (12) between which the piezoelectric/electrostrictive element (20) is sandwiched.

摘要翻译： 提供了能够减少反应时间，应用广泛目标物质，防止失配结合有效并能够进行高精度检测的新型芯片。 因此，本发明的反应性芯片具有固定在布置在支撑件（30）上的三个或更多个振动区域（50）中的每个上的捕获探针（60），捕获探针能够结合到目标物质，其中每个振动区域 具有第一电极（11）的振动产生部（40）和压电/电致伸缩元件（20）夹在其间的第二电极（12）。

公开(公告)号：US07341697B2

公开(公告)日：2008-03-11

申请号：US10377579

申请日：2003-02-28

申请人： Yukihisa Takeuchi ,  Yasuko Yoshida ,  Toshikazu Hirota

发明人： Yukihisa Takeuchi ,  Yasuko Yoshida ,  Toshikazu Hirota

IPC分类号： B01L3/00 ,  G01N29/00

CPC分类号： B01L3/5085 ,  B01F11/0266 ,  B01L2300/0627 ,  B01L2300/0803 ,  B01L2400/0439 ,  G01N11/16 ,  G01N33/54393

摘要： A reaction cell is provided with a vessel-like cell main body and a circular piezoelectric/electrostrictive oscillator fixed to an outside of a bottom surface of the cell main body. The cell main body includes a circular bottom plate portion and a circumferential wall portion which rises from a circumferential edge of the bottom plate portion so as to thereby surround the bottom plate portion. The bottom plate portion and the circumferential wall portion are integrally formed from ceramics. A solution accommodation space is formed above the bottom plate portion and is partially enclosed by the circumferential wall portion. The piezoelectric/electrostrictive oscillator is concentrically adhered to the outside of the bottom surface of the cell main body.

摘要翻译： 反应池设置有容器状电池主体和固定在电池主体的底面外侧的圆形压电/电致伸缩振荡器。 电池主体包括圆形底板部分和从底板部分的周缘上升以便包围底板部分的周壁部分。 底板部和周壁部由陶瓷一体形成。 在底板部的上方形成有溶解容纳空间，并被周壁部分封闭。 压电/电致伸缩振荡器同心地粘附到电池主体的底表面的外部。

公开(公告)号：US20080050285A1

公开(公告)日：2008-02-28

申请号：US11923007

申请日：2007-10-24

申请人： Yukihisa TAKEUCHI ,  Yasuko Yoshida ,  Toshikazu Hirota

发明人： Yukihisa TAKEUCHI ,  Yasuko Yoshida ,  Toshikazu Hirota

IPC分类号： B01L3/00

CPC分类号： B01L3/5085 ,  B01F11/0266 ,  B01L2300/0627 ,  B01L2300/0803 ,  B01L2400/0439 ,  G01N11/16 ,  G01N33/54393

摘要： A reaction cell is provided with a vessel-like cell main body and a circular piezoelectric/electrostrictive oscillator fixed to an outside of a bottom surface of the cell main body. The cell main body includes a circular bottom plate portion and a circumferential wall portion which rises from a circumferential edge of the bottom plate portion so as to thereby surround the bottom plate portion. The bottom plate portion and the circumferential wall portion are integrally formed from ceramics. A solution accommodation space is formed above the bottom plate portion and is partially enclosed by the circumferential wall portion. The piezoelectric/electrostrictive oscillator is concentrically adhered to the outside of the bottom surface of the cell main body.

摘要翻译： 反应池设置有容器状电池主体和固定在电池主体的底面外侧的圆形压电/电致伸缩振荡器。 电池主体包括圆形底板部分和从底板部分的周缘上升以便包围底板部分的周壁部分。 底板部和周壁部由陶瓷一体形成。 在底板部的上方形成有溶解容纳空间，并被周壁部分封闭。 压电/电致伸缩振荡器同心地粘附到电池主体的底表面的外部。

公开(公告)号：US20070238870A1

公开(公告)日：2007-10-11

申请号：US11572506

申请日：2005-07-29

申请人： Mitsuo Kawase ,  Yasuko Yoshida ,  Kazunari Yamada ,  Tomokazu Takase

发明人： Mitsuo Kawase ,  Yasuko Yoshida ,  Kazunari Yamada ,  Tomokazu Takase

IPC分类号： C12M1/00

CPC分类号： B01L3/502746 ,  B01L3/502707 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/165

摘要： The hybridization device of the invention aims to attain a hybridization reaction of high reproducibility. A hybridization device 2 for a hybridization reaction of nucleic acid has a cover member 10 that defines a cavity 12, which includes a nucleic acid fixation area 6 of a substrate 4 for fixation of a nucleic acid probe and has capacity for storage of a liquid for the hybridization reaction therein. At least part of an area exposed to inside of the cavity 12 forms a hydrophobic region 18. Adequate control of the surface characteristic of the area exposed to the cavity for implementing the hybridization reaction desirably enhances the signal intensity and reduces a variation in signal intensity, thus attaining the hybridization reaction of high reproducibility.

摘要翻译： 本发明的杂交装置旨在实现高再现性的杂交反应。 用于核酸杂交反应的杂交装置2具有限定空腔12的覆盖构件10，其包括用于固定核酸探针的基底4的核酸固定区域6，并且具有用于储存用于 其中的杂交反应。 暴露于空腔12内部的区域的至少一部分形成疏水区域18。 为了实现杂交反应，暴露于空腔的区域的表面特性的适当控制期望增强信号强度并降低信号强度的变化，从而实现高再现性的杂交反应。

公开(公告)号：US20050059037A1

公开(公告)日：2005-03-17

申请号：US10795436

申请日：2004-03-09

申请人： Isao Saito ,  Akimitsu Okamoto ,  Yoshio Saito ,  Yasuko Yoshida ,  Kousuke Niwa

发明人： Isao Saito ,  Akimitsu Okamoto ,  Yoshio Saito ,  Yasuko Yoshida ,  Kousuke Niwa

IPC分类号： C07H19/04 ,  C07H19/067 ,  C07H21/00 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/58

CPC分类号： C07H19/04 ,  C07H19/06 ,  C07H19/067 ,  C07H19/16 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6837 ,  Y10S436/809 ,  C12Q2565/549

摘要： A novel nucleotide derivative, in case of existing as a member of a single-stranded sequence, undergoing a change in the fluorescent signal intensity depending on the corresponding base type in the partner strand with which the single-stranded sequence is hybridized, and which is a thymine/uracil derivative (1) emitting light most intensely when a confronting base in the partner strand with which the single-stranded nucleotide sequence is hybridized is adenine; a cytosine derivative (2) emitting light most intensely when the confronting base is guanine; an adenine derivative (3) emitting light most intensely when the confronting base is cytosine; a guanine derivative (4) emitting light most intensely when the confronting base is cytosine or thymine/uracil; and an adenine derivative (5) emitting light most intensely when the confronting base is thymine/uracil.

摘要翻译： 在存在单链序列成员的情况下，新的核苷酸衍生物根据与单链序列杂交的伴侣链中的相应碱基类型经历荧光信号强度的变化，并且其是 当与单链核苷酸序列杂交的伴侣链中的相对的碱基是腺嘌呤时，最强烈地发射光的胸腺嘧啶/尿嘧啶衍生物（1） 当相对的碱是鸟嘌呤时，胞嘧啶衍生物（2）最强地发射光; 当面对的碱基是胞嘧啶时，腺苷衍生物（3）最强地发光; 当面对的碱基是胞嘧啶或胸腺嘧啶/尿嘧啶时，最强烈地发射光的鸟嘌呤衍生物（4） 和当面对的碱是胸腺嘧啶/尿嘧啶时最强烈地发光的腺嘌呤衍生物（5）。