公开(公告)号：US10973227B2

公开(公告)日：2021-04-13

申请号：US15136777

申请日：2016-04-22

Applicant: BERKELEY LIGHTS, INC.

Inventor： Mark P. White ,  Kevin T. Chapman ,  Andrew W. McFarland ,  Eric D. Hobbs ,  Randall D. Lowe, Jr.

IPC: A01N1/02 ,  C12M1/00 ,  B01L3/00

Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.

公开(公告)号：US20200230601A1

公开(公告)日：2020-07-23

申请号：US16683798

申请日：2019-11-14

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: B01L3/00 ,  G01N33/558

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

公开(公告)号：US10646871B2

公开(公告)日：2020-05-12

申请号：US15989549

申请日：2018-05-25

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: G01N33/558 ,  B01L3/00

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

公开(公告)号：US20200078788A1

公开(公告)日：2020-03-12

申请号：US16509166

申请日：2019-07-11

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC: B01L3/00 ,  B03C5/02 ,  B03C5/00 ,  G01N15/14 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68

Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US20190275516A1

公开(公告)日：2019-09-12

申请号：US16196649

申请日：2018-11-20

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, JR. ,  Alexander J. Mastroianni ,  Mark P. White ,  Gregory G. Lavieu ,  Kristin G. Beaumont

IPC: B01L3/00 ,  C12M3/06

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.

公开(公告)号：US20170355595A1

公开(公告)日：2017-12-14

申请号：US15439506

申请日：2017-02-22

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Daniele Malleo ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Alexander H. Slocum ,  Mark P. White

IPC: B81B7/02 ,  B01L3/00 ,  B03C5/02 ,  G01N33/543 ,  B03C5/00 ,  G01N33/50 ,  C12M1/34 ,  G01N27/447

CPC classification number: B81B7/02 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C7/023 ,  B03C2201/26 ,  C12M23/16 ,  C12M41/46 ,  C12M47/02 ,  G01N27/447 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/5023 ,  G01N33/543 ,  G01N33/54326 ,  G01N35/10

Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

公开(公告)号：US20170113231A9

公开(公告)日：2017-04-27

申请号：US14520510

申请日：2014-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Daniele Malleo ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Alexander H. Slocum ,  Mark P. White

IPC: B03C7/02 ,  B81B7/02

CPC classification number: B81B7/02 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C7/023 ,  B03C2201/26 ,  C12M23/16 ,  C12M41/46 ,  C12M47/02 ,  G01N27/447 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/5023 ,  G01N33/543 ,  G01N33/54326 ,  G01N35/10

Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

公开(公告)号：US20170021366A1

公开(公告)日：2017-01-26

申请号：US15105849

申请日：2014-12-18

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Eric D. Hobbs ,  Steven W. Short ,  Mark P. White ,  Daniele Malleo

IPC: B03C5/00 ,  B03C11/00 ,  B01L3/00 ,  B03C5/02

CPC classification number: B03C5/005 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2200/14 ,  B01L2400/0424 ,  B03C5/026 ,  B03C11/00 ,  B03C2201/26

Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

Abstract translation: 可以在微流体装置中选择各种生物细胞，并将其移动到装置中的隔离笔中。 然后，细胞可以在笔中裂解，从而释放核酸物质，其可被笔中的一个或多个捕获物捕获。 然后可以从笔中取出捕获的核酸材料的捕获物体。 捕获对象可以包括唯一的标识符，允许每个捕获对象与由对象捕获的核酸材料源自的单个细胞相关联。

公开(公告)号：US20160370266A1

公开(公告)日：2016-12-22

申请号：US15136763

申请日：2016-04-22

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Randall D. Lowe, JR.

IPC: G01N1/34 ,  B03C5/00 ,  G01N27/447 ,  C12Q1/68 ,  B01L3/00

CPC classification number: G01N1/34 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0454 ,  B01L2400/0457 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6806 ,  G01N27/44704 ,  G01N27/44791

Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.

Abstract translation: 本公开的方面涉及微流体装置中的细胞核的操作以及用于执行这些方法的组合物，系统和试剂盒。 在一些方面，本公开提供了将一个或多个选定的细胞核放置在微流体装置中的隔离笔的隔离区域中的方法。 然后可以从隔离笔的隔离区域中取出分离的核/核，并用于任何所需的下游测定或过程。

公开(公告)号：US20150352547A1

公开(公告)日：2015-12-10

申请号：US14732682

申请日：2015-06-06

Applicant: Berkeley Lights, Inc.

Inventor： Keith Joseph Breinlinger ,  Eric D. Hobbs ,  Dorian Liepmann ,  Joshua Tanner Nevill ,  Mark P. White ,  Maria Jimena Loureiro

IPC: B01L3/00 ,  C12M3/06

CPC classification number: B01L3/5027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/086 ,  C12M23/16 ,  Y10T436/2575

Abstract: Some configurations of a microfluidic apparatus can comprise a fluidic circuit of interconnected fluidic structures into which a plurality of different media can be introduced or extracted. A variety of operations can be performed with the different media including isolating with a second medium one or more of the fluidic structures that is filled partially or fully with a first medium. Discrete volumes of a medium can be moved through the isolating second medium to deliver materials or micro-objects to or remove micro-objects or materials from a fluidic structure that is otherwise isolated by the second medium. Some configurations of a microfluidic apparatuses can isolate microfluidic structures in a microfluidic apparatus using flow rates or blocking structures, and some configurations can manage bubbles in fluidic structures.

Abstract translation: 微流体装置的一些构造可以包括互连流体结构的流体回路，多个不同介质可以被引入或提取到流体结构中。 可以使用不同的介质进行各种操作，包括用第二介质隔离一种或多种用第一介质部分或完全填充的流体结构。 介质的离散体积可以移动通过隔离的第二介质，以将材料或微物体输送到或从其被第二介质分离的流体结构中去除微物体或材料。 微流体装置的一些构造可以使用流速或阻塞结构隔离微流体装置中的微流体结构，并且一些配置可以管理流体结构中的气泡。