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公开(公告)号:US20090137413A1
公开(公告)日:2009-05-28
申请号:US11928808
申请日:2007-10-30
申请人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
发明人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
IPC分类号: C40B30/04
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.
摘要翻译: 可流动或固定的颗粒组的数组用于微流体系统中,用于进行测定和改变流体动力学流动。 还提供了利用微流体系统内的可流动或固定的颗粒组的测定法,以及包括阵列和阵列构件的试剂盒,装置和集成系统。
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42.
公开(公告)号:US06787088B2
公开(公告)日:2004-09-07
申请号:US10316571
申请日:2002-12-10
IPC分类号: B29C4516
CPC分类号: B01L3/502707 , B01J19/0093 , B01J2219/00783 , B01J2219/00833 , B01J2219/00853 , B01J2219/00952 , B01J2219/00995 , B01L3/50273 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0816 , B01L2300/0887 , B01L2300/12 , B01L2400/0418 , G01N27/44791 , Y10S366/03 , Y10T29/49229 , Y10T137/2224
摘要: Microfluidic devices are provided for the performance of chemical and biochemical analyzes, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have broad applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields.
摘要翻译: 提供微流控器件用于化学和生物化学分析,合成和检测的性能。 本发明的装置将精确的流体控制系统与微制造的聚合物基底结合在一起,以提供精确,低成本的小型化分析装置,其在化学,生物化学,生物技术,分子生物学和许多其它领域中具有广泛的应用。
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公开(公告)号:US06537748B1
公开(公告)日:2003-03-25
申请号:US09258133
申请日:1999-02-26
IPC分类号: C12Q168
CPC分类号: C12Q1/6827 , C12Q1/6858 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2535/125 , C12Q2535/101 , C12Q2563/107 , C12Q2537/143 , C12Q2525/121
摘要: This invention concerns a reagent composition comprising at least two different terminators of a nucleic acid template-dependent, primer extension reaction. This invention also concerns a method for determining the identity of a nucleotide base at a specific position in a nucleic acid of interest. This invention further concerns a method for determining the presence or absence of a particular nucleotide sequence in a sample of nucleic acids. This invention further concerns a method for identifying different alleles in a sample containing nucleic acids. This invention further concerns a method for determining the genotype of an organism at one or more particular genetic loci.
摘要翻译: 本发明涉及包含核酸模板依赖性引物延伸反应的至少两种不同终止子的试剂组合物。 本发明还涉及用于确定感兴趣的核酸中特定位置的核苷酸碱基的身份的方法。 本发明还涉及用于确定核酸样品中特定核苷酸序列的存在或不存在的方法。 本发明还涉及用于鉴定含有核酸的样品中的不同等位基因的方法。 本发明还涉及用于确定一个或多个特定遗传基因座上的生物体的基因型的方法。
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公开(公告)号:US06482364B2
公开(公告)日:2002-11-19
申请号:US09848717
申请日:2001-05-03
申请人: J. Wallace Parce , Michael R. Knapp
发明人: J. Wallace Parce , Michael R. Knapp
IPC分类号: B01L302
CPC分类号: B01F13/0071 , B01F13/0084 , B01F13/0086 , B01J19/0093 , B01J2219/00891 , B01L3/021 , B01L3/502715 , B01L3/50273 , B01L3/502784 , B01L2200/027 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44743 , G01N27/44791 , H02N11/006 , Y10S366/02 , Y10T436/113332 , Y10T436/2575
摘要: The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.
摘要翻译: 本发明提供了使用电动力通过微流体系统的通道输送材料的技术。 主体材料在高离子浓度的区域运输,靠近高离子浓度的间隔物材料区域,其由具有低离子浓度的间隔物材料区域分离。 这种布置允许材料在运输通过时间内保持局部化,以避免混合材料。 使用这些技术,产生与微流体系统相容的电吸移器,使得材料可以容易地引入到微流体系统中。 本发明还补偿电泳偏压,因为材料通过将通道分成具有正表面电荷和负表面电荷的部分,以及在两部分之间的第三电极,或者通过在运输之后电泳材料的扩散而传输通过微流体系统的通道 一个频道
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公开(公告)号:US06409900B1
公开(公告)日:2002-06-25
申请号:US09664847
申请日:2000-09-19
IPC分类号: B44C122
CPC分类号: B01L3/502707 , B01J19/0093 , B01J2219/00783 , B01J2219/00833 , B01J2219/00853 , B01J2219/00952 , B01J2219/00995 , B01L3/50273 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0816 , B01L2300/0887 , B01L2300/12 , B01L2400/0418 , G01N27/44791 , Y10S366/03 , Y10T29/49229 , Y10T137/2224
摘要: Microfluidic devices are provided for the performance of chemical and biochemical analyses, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have broad applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields.
摘要翻译: 提供微流控器件用于化学和生物化学分析,合成和检测的性能。 本发明的装置将精确的流体控制系统与微制造的聚合物基底结合在一起,以提供精确,低成本的小型化分析装置,其在化学,生物化学,生物技术,分子生物学和许多其它领域中具有广泛的应用。
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公开(公告)号:US06287520B1
公开(公告)日:2001-09-11
申请号:US09488801
申请日:2000-01-21
申请人: J. Wallace Parce , Michael R. Knapp
发明人: J. Wallace Parce , Michael R. Knapp
IPC分类号: B01L302
CPC分类号: B01F13/0071 , B01F13/0084 , B01F13/0086 , B01J19/0093 , B01J2219/00891 , B01L3/021 , B01L3/502715 , B01L3/50273 , B01L3/502784 , B01L2200/027 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44743 , G01N27/44791 , H02N11/006 , Y10S366/02 , Y10T436/113332 , Y10T436/2575
摘要: The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.
摘要翻译: 本发明提供了使用电动力通过微流体系统的通道输送材料的技术。 主体材料在高离子浓度的区域运输,靠近高离子浓度的间隔物材料区域,其由具有低离子浓度的间隔物材料区域分离。 这种布置允许材料在运输通过时间内保持局部化,以避免混合材料。 使用这些技术,产生与微流体系统相容的电吸移器,使得材料可以容易地引入到微流体系统中。 本发明还补偿电泳偏压,因为材料通过将通道分成具有正表面电荷和负表面电荷的部分,以及两部分之间的第三电极,或者通过在运输之后通过电泳材料的扩散而传输通过微流体系统的通道 一个频道
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公开(公告)号:US5885470A
公开(公告)日:1999-03-23
申请号:US843212
申请日:1997-04-14
IPC分类号: B01J19/00 , B01L3/00 , G01N27/447 , B44C1/22
CPC分类号: B01L3/502707 , B01J19/0093 , B01L3/50273 , G01N27/44791 , B01J2219/00783 , B01J2219/00833 , B01J2219/00853 , B01J2219/00952 , B01J2219/00995 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0816 , B01L2300/0887 , B01L2300/12 , B01L2400/0418 , Y10S366/03 , Y10T137/2224 , Y10T29/49229
摘要: Microfluidic devices are provided for the performance of chemical and biochemical analyses, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have broad applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields.
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公开(公告)号:US5880071A
公开(公告)日:1999-03-09
申请号:US760446
申请日:1996-12-06
申请人: J. Wallace Parce , Michael R. Knapp
发明人: J. Wallace Parce , Michael R. Knapp
IPC分类号: B01F13/00 , B01J19/00 , B01L3/00 , B01L3/02 , F04B17/00 , F04B19/00 , G01N27/447 , H02N11/00 , G01N27/26
CPC分类号: B01F13/0071 , B01J19/0093 , B01L3/502715 , B01L3/50273 , B01L3/502784 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44743 , G01N27/44791 , H02N11/006 , B01F13/0084 , B01F13/0086 , B01L2200/027 , B01L2200/0673 , B01L2300/0816 , B01L2400/0415 , B01L3/021 , B01L3/502753
摘要: The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.
摘要翻译: 本发明提供了使用电动力通过微流体系统的通道输送材料的技术。 主体材料在高离子浓度的区域内运输,靠近高离子浓度的间隔物材料区域,其由具有低离子浓度的间隔物材料区域分离。 这种布置允许材料在运输通过时间内保持局部化,以避免混合材料。 使用这些技术,产生与微流体系统相容的电吸移器,使得材料可以容易地引入到微流体系统中。 本发明还补偿电泳偏压,因为材料通过将通道分成具有正表面电荷和负表面电荷的部分,以及两部分之间的第三电极,或者通过在运输之后电泳材料的扩散而传输通过微流体系统的通道 一个频道
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公开(公告)号:US5610287A
公开(公告)日:1997-03-11
申请号:US341148
申请日:1994-11-16
申请人: Theo Nikiforov , Michael R. Knapp
发明人: Theo Nikiforov , Michael R. Knapp
CPC分类号: C12Q1/6834 , C12N15/1006
摘要: Synthetic nucleic acid molecules are non-covalently immobilized in the presence of a salt or cationic detergent on a hydrophilic polystyrene solid support containing an --OH, --C.dbd.O or --COOH hydrophilic group or on a glass solid support. The support is contacted with a solution having a pH of about 6 to about 8 containing the synthetic nucleic acid and the cationic detergent or salt. Preferably, the cationic detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride at a concentration of about 30 mM to about 100 mM or octyldimethylamine hydrochloride at a concentration of about 50 mM to about 150 mM. The salt is preferably NaCl at a concentration of about 50 mM to about 250 mM. When the detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride, the glass support or the hydrophilic polystyrene support is used. When NaCl or octyldimethylamine hydrochloride is used, the support is the hydrophilic polystyrene. After immobilization, the support containing the immobilized nucleic acid may be washed with an aqueous solution containing a non-ionic detergent. The immobilized nucleic acid may be used in nucleic acid hybridization assays, nucleic acid sequencing and in analysis of genomic polymorphisms.
摘要翻译: 在含有-OH,-C = O或-COOH亲水基团的亲水性聚苯乙烯固体载体上或在玻璃固体载体上,在盐或阳离子洗涤剂存在下,合成核酸分子非共价固定。 将载体与含有合成核酸和阳离子洗涤剂或盐的pH约6至约8的溶液接触。 优选地,阳离子洗涤剂是浓度为约30mM至约100mM的1-乙基-3-(3'-二甲基氨基丙基)-1,3-碳二亚胺氯化物或浓度为约50mM至约150mM的辛基二甲基胺盐酸盐 。 该盐优选浓度为约50mM至约250mM的NaCl。 当洗涤剂为1-乙基-3-(3'-二甲基氨基丙基)-1,3-碳二亚胺氯化物时,使用玻璃载体或亲水性聚苯乙烯载体。 当使用NaCl或辛基二甲胺盐酸盐时,载体是亲水性聚苯乙烯。 固定后,含有固定化核酸的载体可以用含有非离子型洗涤剂的水溶液洗涤。 固定核酸可用于核酸杂交测定,核酸测序和基因组多态性分析。
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公开(公告)号:US5518900A
公开(公告)日:1996-05-21
申请号:US155746
申请日:1993-11-23
IPC分类号: C12N15/09 , C07H21/04 , C12N15/10 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , C12N15/00 , C12P19/34
CPC分类号: C12Q1/686 , C12N15/10 , C12Q1/6848 , C12Q1/6853 , C12Q1/6858 , Y10S435/81
摘要: A method for generating single-stranded nucleic acid molecules. The molecules contain nuclease resistant modified nucleotides, such that they are resistant to 5'.fwdarw.3' exonucleases.
摘要翻译: 一种产生单链核酸分子的方法。 分子含有核酸酶抗性修饰的核苷酸,使得它们对5'→3'外切核酸酶具有抗性。
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