公开(公告)号：US20160257976A1

公开(公告)日：2016-09-08

申请号：US14889543

申请日：2014-05-02

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Hong-Weon Lee ,  Jung Oh Ahn ,  Joon Ki Jung ,  Hee-Ju Ko ,  Sun-Joo Park ,  Chun Sug Kim ,  Hyeok Won Lee ,  Eun Gyo Lee ,  Joo Hwan Lee

IPC: C12P13/00 ,  C12N15/70 ,  C07D223/10

CPC classification number: C12P13/005 ,  C07D223/10 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P17/10

Abstract: The present invention relates to a method for preparing a recombinant microorganism simultaneously comprising genes encoding enzymes used in the biosynthesis pathway of 6-aminocaproic acid, which is a precursor of caprolactam, biosynthesizing 6-aminocaproic acid from the microorganism, and producing the same so as to synthesize caprolactam.

Abstract translation: 本发明涉及一种同时制备重组微生物的方法，该方法同时包含编码在6-氨基己酸的生物合成途径中使用的酶的基因，该氨基己酸是己内酰胺的前体，从微生物中合成6-氨基己酸， 合成己内酰胺。

公开(公告)号：US20160097096A1

公开(公告)日：2016-04-07

申请号：US14806514

申请日：2015-07-22

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Jeong Ki MIN ,  Jongjin PARK ,  Kwang-Hee BAE ,  Sang Chul LEE ,  Yee Sook CHO

IPC: C12Q1/68 ,  C07K16/28 ,  G01N33/569

CPC classification number: C12Q1/6881 ,  C07K16/28 ,  C12Q2600/158 ,  G01N33/56966 ,  G01N2333/705 ,  G01N2400/00

Abstract: The present invention relates to a composition for detecting the undifferentiated human pluripotent stem cells comprising an agent useful for measuring the level of Desmoglein 2 (Dsg 2) mRNA or the protein thereof, a kit for detecting the undifferentiated human pluripotent stem cells comprising the said composition, a method for detecting the undifferentiated human pluripotent stem cells containing the step of measuring the level of Desmoglein 2 mRNA or the protein thereof, a method for evaluating the differentiation of human pluripotent stem cells and thereafter for separating the undifferentiated human pluripotent stem cells, a method for reducing the undifferentiated status of human pluripotent stem cells by inhibiting the expression or activation of Desmoglein 2, and a monoclonal antibody binding specifically to human Desmoglein 2.

Abstract translation: 本发明涉及一种用于检测未分化的人多能干细胞的组合物，其包含可用于测定脱骨髓糖蛋白2（Dsg2）mRNA或其蛋白质的水平的试剂，用于检测未分化的人多能干细胞的试剂盒，所述试剂盒包含所述组合物 用于检测未分化的人多能干细胞的方法，所述方法包括测定脱骨髓蛋白素2 mRNA或其蛋白质的水平的步骤，评估人多能干细胞分化的方法，然后分离未分化的人多能干细胞， 通过抑制脱骨髓素蛋白2的表达或活化来减少人多能干细胞未分化状态的方法，以及特异性结合人骨髓蛋白2的单克隆抗体。

公开(公告)号：US20160095845A1

公开(公告)日：2016-04-07

申请号：US14876509

申请日：2015-10-06

Applicant: Byoung Mog KWON ,  Dong Cho HAN ,  Sang Ku LEE ,  Yu Jin LEE ,  Yoon Jung JEON ,  Jiyeon CHOI ,  Ah-Rum HA ,  Young Min HAN

Inventor： Byoung Mog KWON ,  Dong Cho HAN ,  Sang Ku LEE ,  Yu Jin LEE ,  Yoon Jung JEON ,  Jiyeon CHOI ,  Ah-Rum HA ,  Young Min HAN

IPC: A61K31/4453 ,  A61K45/06

CPC classification number: A61K31/4453 ,  A61K45/06

Abstract: The present invention relates to a pharmaceutical composition for preventing or treating cancer, comprising (S)-(−)-benproperine or a pharmaceutically acceptable salt thereof as an active ingredient, and a method for preventing or treating cancer using the same.

Abstract translation: 本发明涉及用于预防或治疗癌症的药物组合物，其包含（S） - （ - ） - 苯丙氨酸或其药学上可接受的盐作为活性成分，以及使用其的预防或治疗癌症的方法。

公开(公告)号：US09266872B2

公开(公告)日：2016-02-23

申请号：US14256808

申请日：2014-04-18

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Semi Kim ,  Ill Young Lee ,  Hye-Jin Min ,  Eun-Hee Nam ,  Pilho Kim ,  Chang Soo Yun ,  Dong Joon Ko

IPC: A61K31/167 ,  A61K31/426 ,  C07D417/04 ,  C07D215/38 ,  C07D285/135 ,  C07D213/75 ,  C07D239/42 ,  C07D277/46 ,  C07D213/82 ,  C07D215/56 ,  C07D277/82 ,  A61K31/4439 ,  A61K31/428 ,  A61K31/433 ,  A61K31/44 ,  A61K31/4709 ,  A61K31/505 ,  A61K31/5377

CPC classification number: C07D417/04 ,  A61K31/167 ,  A61K31/426 ,  A61K31/428 ,  A61K31/433 ,  A61K31/44 ,  A61K31/4439 ,  A61K31/4709 ,  A61K31/505 ,  A61K31/5377 ,  C07D213/75 ,  C07D213/82 ,  C07D215/38 ,  C07D215/56 ,  C07D239/42 ,  C07D277/46 ,  C07D277/82 ,  C07D285/135

Abstract: The present invention relates to a 2-hydroxyarylamide derivative or a pharmaceutically acceptable salt thereof, a preparation method thereof, and a pharmaceutical composition for preventing or treating cancer comprising the same as an active ingredient. The 2-hydroxyarylamide derivative prepared by the present invention is excellent in the inhibition of the activity of TMPRSS4 serine protease and the suppression of the infiltration of TMPRSS4-expressed cancer cells, and thus can be useful as a composition for preventing or treating cancer by inhibiting TMPRSS4 over-expressed in cancer cells, particularly, colorectal cancer, lung cancer, breast cancer, prostate cancer, ovarian cancer, pancreatic cancer, or stomach cancer cells.

Abstract translation: 本发明涉及2-羟基芳基酰胺衍生物或其药学上可接受的盐，其制备方法和用于预防或治疗癌症的药物组合物，其包含其作为活性成分。 通过本发明制备的2-羟基芳基酰胺衍生物对TMPRSS4丝氨酸蛋白酶的活性的抑制和TMPRSS4表达的癌细胞的浸润的抑制是优异的，因此可以用作通过抑制来预防或治疗癌症的组合物 TMPRSS4在癌细胞，特别是结肠直肠癌，肺癌，乳腺癌，前列腺癌，卵巢癌，胰腺癌或胃癌细胞中过度表达。

公开(公告)号：US20150299733A1

公开(公告)日：2015-10-22

申请号：US14111721

申请日：2012-04-13

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Jung Hoon Sohn ,  Hyun Jin Kim ,  Chul Ho Kim ,  Ji Hun Seomoon ,  Bong Hyun Sung ,  Kwang Mook Lim ,  Mi Jin Kim ,  Jong Hyun Kim

IPC: C12P7/06 ,  C12N9/42

CPC classification number: C12P7/06 ,  C12N9/2445 ,  C12Y302/01021 ,  Y02E50/17

Abstract: The present invention relates to beta-glucosidase that is mutated to have enhanced activity, and a method for producing bioethanol using the same. More particularly, the present invention relates to a polynucleotide encoding beta-glucosidase that is mutated to have enhanced activity, beta-glucosidase expressed from the polynucleotide, an expression vector including the polynucleotide, a transformant that is transformed with the expression vector, a method for producing the mutated beta-glucosidase using the transformant, and a method for producing bioethanol using the transformant. The mutant beta-glucosidase of the present invention increases production of glucose much more than the conventional beta-glucosidase, and thus it can be widely used for economic production of bioethanol.

Abstract translation: 本发明涉及突变以提高活性的β-葡糖苷酶，以及使用其生产生物乙醇的方法。 更具体地说，本发明涉及一种突变为具有增强活性的β-葡糖苷酶的多核苷酸，由多核苷酸表达的β-葡糖苷酶，包含多核苷酸的表达载体，用表达载体转化的转化体， 使用转化体产生突变的β-葡糖苷酶，以及使用转化体生产生物乙醇的方法。 本发明的突变型β-葡糖苷酶比常规的β-葡糖苷酶增加葡萄糖的产量，因此可广泛用于生物乙醇的经济生产。

公开(公告)号：US20150299665A1

公开(公告)日：2015-10-22

申请号：US14340991

申请日：2014-07-25

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Yee Sook Cho ,  Chae Wha Yoo ,  Hee Jun Na

IPC: C12N5/074 ,  A61K35/12 ,  C12N5/071

CPC classification number: C12N5/0696 ,  A61K35/32 ,  A61K35/44 ,  C12N5/069 ,  C12N5/0691 ,  C12N5/0692 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2506/1361 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2533/90

Abstract: The present invention relates to a method for producing endothelial progenitor cells, comprising a method for producing induced pluripotent stem cells by using only Oct4 and Sox2 as a reprogramming factor of human dental pulp cells, and differentiating endothelial progenitor cells from the induced pluripotent stem cells produced by the method for producing induced pluripotent stem cells. Moreover, the present invention relates to a method for producing endothelial cells, comprising differentiating endothelial cells from endothelial progenitor cells produced by the method for producing the endothelial progenitor cells. Additionally, the present invention relates to a method for producing smooth muscle cells, comprising differentiating smooth muscle cells from endothelial progenitor cells produced by the method for producing the endothelial progenitor cells.Also, the present invention make a significant contribution to treatment of ischemia disease and heart disease because a patient's dental pulp cell-derived differentiated cells can be used for cell treatment as a result of the cell treatment effect of the endothelial progenitor cells in a mouse model induced to have hind limb ischemia or myocardial infarction.

Abstract translation: 本发明涉及一种生产内皮祖细胞的方法，其包括通过仅使用Oct4和Sox2作为人牙髓细胞的重新编程因子来产生诱导的多能干细胞的方法，以及从产生的诱导多能干细胞分化内皮祖细胞 通过产生诱导多能干细胞的方法。 此外，本发明涉及一种生产内皮细胞的方法，其包括将内皮细胞与由内皮祖细胞产生的方法产生的内皮祖细胞分化。 此外，本发明涉及一种平滑肌细胞的制备方法，其包括通过用于产生内皮祖细胞的方法产生的内皮祖细胞分化平滑肌细胞。 此外，本发明对于缺血性疾病和心脏病的治疗有显着的贡献，因为患者的牙髓细胞衍生的分化细胞可以作为小鼠模型中内皮祖细胞的细胞处理效果的细胞治疗而使用 诱发后肢缺血或心肌梗死。

公开(公告)号：US09090919B2

公开(公告)日：2015-07-28

申请号：US13505111

申请日：2009-10-29

Applicant: Chul-Ho Kim ,  Jeong-Woo Seo ,  Lianhua Luo ,  Baek Rock Oh ,  Pil-Soo Seo ,  Sun-Yeon Heo

Inventor： Chul-Ho Kim ,  Jeong-Woo Seo ,  Lianhua Luo ,  Baek Rock Oh ,  Pil-Soo Seo ,  Sun-Yeon Heo

IPC: C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C07K14/26 ,  C12N9/02 ,  C12N9/04

CPC classification number: C12P7/42 ,  C07K14/26 ,  C12N9/0006 ,  C12N9/0008 ,  C12P7/18 ,  C12P7/52

Abstract: The present invention relates to a novel method of producing 3-hydroxypropionic acid from glycerol, and more particularly to a method of producing 3-hydroxypropionic acid by culturing in a glycerol-containing medium a mutant microorganism obtained by amplifying an aldehyde dehydrogenase-encoding gene in a microorganism having the abilities to produce coenzyme B12 and produce 3-hydroxypropionic acid using glycerol as a carbon source. The present invention enables the fermentation of glycerol even under microaerobic or aerobic conditions without having to add coenzyme B12. Thus, the invention will be very suitable for the development of biological processes for producing large amounts of 3-hydroxypropionic acid.

Abstract translation: 本发明涉及一种由甘油生产3-羟基丙酸的新方法，更具体地说，涉及一种通过在含甘油培养基中培养产生3-羟基丙酸的方法，该突变体微生物是通过扩增醛脱氢酶编码基因 具有生产辅酶B12并使用甘油作为碳源产生3-羟基丙酸的微生物。 本发明即使在微需氧或需氧条件下也可以发酵甘油，而不必加入辅酶B12。 因此，本发明将非常适用于生产大量3-羟基丙酸的生物方法的开发。

公开(公告)号：US09068010B2

公开(公告)日：2015-06-30

申请号：US13699104

申请日：2011-10-26

Applicant: Sung Uk Kim ,  Ingyu Hwang ,  Byoung-Keun Park ,  Seungeun Kim ,  Jae Sun Moon ,  Ho Yong Park ,  Kwang Hee Son ,  Tae Sook Jeong

Inventor： Sung Uk Kim ,  Ingyu Hwang ,  Byoung-Keun Park ,  Seungeun Kim ,  Jae Sun Moon ,  Ho Yong Park ,  Kwang Hee Son ,  Tae Sook Jeong

IPC: C07K14/32 ,  C12N9/00 ,  C12P21/02 ,  C12N15/52

CPC classification number: C07K14/32 ,  C12N9/00 ,  C12N15/52 ,  C12P21/02

Abstract: The present invention relates to novel iturin biosynthesis genes, and uses thereof. More specifically, the present invention provides novel iturin biosynthesis genes, wherein the iturin biosynthesis genes were cloned from Bacillus subtilis subsp. krictiensis ATCC 55079, the base sequence was determined after checking whether the cloned genes are iturin biosynthesis genes or not, and it was ascertained that the identified genes are novel genes different from the reported gene by comparing the base sequences with that of the reported gene, and uses thereof.

Abstract translation: 本发明涉及新颖的神经细胞生物合成基因及其用途。 更具体地，本发明提供了新颖的神经细胞生物合成基因，其中从枯草芽孢杆菌亚种克隆了神经元生物合成基因。 克氏杆菌ATCC 55079，检查克隆基因是否是神经元生物合成基因，确定基因序列，通过比较基因序列与报道基因的基因序列，确定鉴定的基因是与报道基因不同的新基因， 及其用途。

公开(公告)号：US09034327B2

公开(公告)日：2015-05-19

申请号：US13322304

申请日：2009-09-07

Applicant: Kyung Sook Chung ,  Mi Sun Won ,  Ji Won Ahn ,  Jeong Hae Choi ,  Hyang Sook Yoo ,  Young Il Yeom ,  Eun Young Song ,  Hee Gu Lee ,  Jae Hun Cheong ,  Chang Mo Kang

Inventor： Kyung Sook Chung ,  Mi Sun Won ,  Ji Won Ahn ,  Jeong Hae Choi ,  Hyang Sook Yoo ,  Young Il Yeom ,  Eun Young Song ,  Hee Gu Lee ,  Jae Hun Cheong ,  Chang Mo Kang

IPC: G01N33/53 ,  C07K14/705 ,  G01N33/50

CPC classification number: C07K14/705 ,  G01N33/5041

Abstract: Disclosed are novel GPCR (G Protein Coupled Receptor) proteins and genes encoding the same. Also provided is the use of the proteins and the genes. Particularly, contemplated are a novel GPCR (G Protein Coupled Receptor) polypeptide, a polynucleotide coding for the same, a recombinant vector carrying the polynucleotide or a fragment thereof, host cells transformed with the vector, a transgenic animal infected with the vector. Also, a composition for detecting a cancer marker, comprising an agent capable of measuring the expression level of mRNA or protein of the GPCR polynucleotide, a kit for the diagnosis of cancer, comprising the composition, and a method for detecting the GPCR polypeptide and a gene encoding the polypeptide are provided. Further, a composition for the treatment and prevention of cancer, comprising an oligonucleotide inhibiting the expression of a gene encoding the GPCR polypeptide or an antibody against the GPCR protein, and a method for screening a modulator of the GPCR protein or a cancer therapeutic agent are provided.

Abstract translation: 公开了新型GPCR（G蛋白偶联受体）蛋白和编码其的基因。 还提供了蛋白质和基因的使用。 特别地，考虑了新型GPCR（G蛋白偶联受体）多肽，编码它的多核苷酸，携带多核苷酸或其片段的重组载体，用该载体转化的宿主细胞，用载体感染的转基因动物。 另外，含有能够测定GPCR多核苷酸的mRNA或蛋白质的表达量的试剂，癌症诊断用试剂盒，含有该组合物的检测癌标记物的组合物，以及检测GPCR多肽的方法和 提供编码多肽的基因。 此外，用于治疗和预防癌症的组合物包含抑制GPCR多肽或编码GPCR蛋白的抗体表达的寡核苷酸以及筛选GPCR蛋白或癌症治疗剂的调节剂的方法， 提供。

公开(公告)号：US08974837B2

公开(公告)日：2015-03-10

申请号：US13427334

申请日：2012-03-22

Applicant: Hyeong Kyu Lee ,  Sei Ryang Oh ,  Kyung Seop Ahn ,  Sang Ku Lee ,  Joong Ku Lee ,  Ok Kyoung Kwon ,  Doo Young Kim ,  Hyouk Joung ,  Gui Hua Quan ,  Mi Jin Kim ,  Bo Young Park

Inventor： Hyeong Kyu Lee ,  Sei Ryang Oh ,  Kyung Seop Ahn ,  Sang Ku Lee ,  Joong Ku Lee ,  Ok Kyoung Kwon ,  Doo Young Kim ,  Hyouk Joung ,  Gui Hua Quan ,  Mi Jin Kim ,  Bo Young Park

IPC: A61K36/00 ,  A61K36/185 ,  A23L1/30 ,  A61K31/35 ,  A61K31/352 ,  A61K36/68 ,  A61K36/80 ,  C07H17/04

CPC classification number: A61K36/185 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/35 ,  A61K31/352 ,  A61K36/68 ,  A61K36/80 ,  C07H17/04 ,  A23V2250/21 ,  A23V2200/324 ,  A23V2200/304

Abstract: The present invention relates to a composition comprising an extract of Pseudolysimachion genus plant, and the catalpol derivatives isolated therefrom having anti-inflammatory, anti-allergic and anti-asthmatic activity. The extract of Pseudolysimachion genus plant and the catalpol derivatives isolated therefrom shows potent suppressing effect on elevated IgE, IL-4 and IL-13 levels and eosinophilia in the plasma and BALF, and mucus overproduction in the lung tissues in an OVA-induced asthmatic mouse model. Therefore, it can be used as the therapeutics or functional health food for treating and preventing inflammatory, allergic and asthmatic disease.

Abstract translation: 本发明涉及包含假单胞菌属植物提取物和从其中分离出的具有抗炎，抗过敏和抗哮喘活性的梓醇衍生物的组合物。 假单胞菌属植物的提取物和从其中分离的梓醇衍生物显示出对OVA诱导的哮喘小鼠肺组织中升高的IgE，IL-4和IL-13水平以及血浆和BALF中的嗜酸性粒细胞增多和粘液过度产生的有效抑制作用 模型。 因此，它可以用作用于治疗和预防炎性，过敏性和哮喘性疾病的治疗剂或功能性保健食品。