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    NUCLEOTIDE ANALOGS FOR REAL-TIME SEQUENCING METHOD FOR SINGLE MOLECULE
    47.
    发明申请
    NUCLEOTIDE ANALOGS FOR REAL-TIME SEQUENCING METHOD FOR SINGLE MOLECULE 审中-公开
    用于单分子实时测序方法的核素模拟

    公开(公告)号：US20170073752A1

    公开(公告)日：2017-03-16

    申请号：US15361083

    申请日：2016-11-25

    申请人： Personal Genomics, Inc.

    发明人： Tsu-Ju Fu ,  Shang-Chia Chang ,  Jenn-Yeh Fann

    IPC分类号： C12Q1/68

    CPC分类号： C07H19/10 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2525/113 ,  C12Q2563/107

    摘要： This invention is to describe nucleotide analogs used in a method for the determination of a nucleic acid sequence. The method involves an enzyme, an enzyme complex or plural number of enzymes with more than one enzymatic activity and a set of nucleotide analogs, to achieve high signal readout accuracy in nucleic acid sequencing by making each signal to have a long signaling time span which allows a higher signal clarity.

    摘要翻译： 本发明是描述用于确定核酸序列的方法中使用的核苷酸类似物。 该方法包括酶，酶复合物或具有多于一种酶活性的多种酶和一组核苷酸类似物，以通过使每个信号具有长的信号时间跨度来实现核酸测序中的高信号读出准确度，这允许 更高的信号清晰度。

    Nucleotide and/or oligonucleotide and preparation process thereof
    49.
    发明授权
    Nucleotide and/or oligonucleotide and preparation process thereof 有权
    核苷酸和/或寡核苷酸及其制备方法

    公开(公告)号：US09567364B2

    公开(公告)日：2017-02-14

    申请号：US13811295

    申请日：2011-07-20

    申请人： Zhen Xi ,  Zicai Liang ,  Jinyu Huang

    发明人： Zhen Xi ,  Zicai Liang ,  Jinyu Huang

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C07H21/00 ,  C07H19/04 ,  C07H19/20 ,  C07H19/10 ,  C07H19/048 ,  C07H19/167

    CPC分类号： C07H21/02 ,  C07H19/10 ,  C07H19/167 ,  C07H19/20 ,  C07H21/04 ,  Y02P20/55

    摘要： Nucleotide and/or oligonucleotide represented by formula (1) and the liquid phase synthesis process thereof. The present invention provides a liquid phase synthesis process for preparing a nucleotide and/or an oligonucleotide, comprising a process for combining the nucleotide and/or oligonucleotide protective groups, in which, under the condition that the 2′-hydroxyl group is protected by a group with a sterically hindered silane structure, the 3′ phosphate group(s) of the nucleotide and/or oligonucleotide is/are directly protected by (a)β-cyanoethyl group(s), and after the β-cyanoethyl group(s) is/are removed, the resulting product can directly participate in the next cycle of synthesis, wherein the synthesis reaction is carried out in a reaction flask or reaction kettle, without being limited by a solid carrier or synthesizer, so that the large scale preparation of oligonucleotides can be achieved.

    摘要翻译： 由式（1）表示的核苷酸和/或寡核苷酸及其液相合成方法。 本发明提供了制备核苷酸和/或寡核苷酸的液相合成方法，包括将核苷酸和/或寡核苷酸保护基组合的方法，其中在2'-羟基被 具有空间位阻硅烷结构的基团，核苷酸和/或寡核苷酸的3'磷酸基团被（a）2-氰基乙基直接保护，并且在2-氰基乙基之后， 所得产物可以直接参与下一个合成循环，其中合成反应在反应烧瓶或反应釜中进行，而不受固体载体或合成仪的限制，从而大规模制备 可以实现寡核苷酸。