公开(公告)号：US20170321235A1

公开(公告)日：2017-11-09

申请号：US15533215

申请日：2015-12-25

Applicant: KIKKOMAN CORPORATION

Inventor： Seiichi HARA ,  Keiko KUROSAWA ,  Keiichi ICHIKAWA

IPC: C12P13/04 ,  C12N9/10

CPC classification number: C12P13/04 ,  C12N9/10 ,  C12N9/1007 ,  C12N9/13 ,  C12N15/09 ,  C12R1/66 ,  C12R1/665 ,  C12R1/685 ,  C12R1/69 ,  C12Y201/01 ,  C12Y208/01007

Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5′-phosphate as a coenzyme.

公开(公告)号：US20170088872A1

公开(公告)日：2017-03-30

申请号：US15242525

申请日：2016-08-20

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Warren Lau ,  Elizabeth Sattely

IPC: C12P19/60 ,  C12N9/02 ,  C12N9/10

CPC classification number: C12Y114/21 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/0073 ,  C12N9/1007 ,  C12P17/181 ,  C12Y102/01 ,  C12Y103/05001 ,  C12Y114/11 ,  C12Y114/13 ,  C12Y201/01 ,  C12Y201/01042

Abstract: The present invention provides compositions and methods for biosynthetically producing podophyllotoxin intermediates and derivatives including enzymes and their equivalents involved in the biosynthetic production of podophyllotoxin intermediates and derivatives.

公开(公告)号：US20160376663A1

公开(公告)日：2016-12-29

申请号：US15121725

申请日：2015-02-27

Applicant: IGENOMX INTERNATIONAL GENOMICS CORPORATION

Inventor： Keith Brown

IPC: C12Q1/68 ,  G06F19/00 ,  A61K31/7072 ,  A61K31/7056 ,  A61K49/00 ,  A61K38/45

CPC classification number: C12Q1/6886 ,  A61K31/7056 ,  A61K31/7072 ,  A61K38/45 ,  A61K49/0054 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/178 ,  C12Y201/01 ,  G16H15/00 ,  Y02A90/26 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/165

Abstract: Methods and compositions related to the use of Mobile Element Insertions and their adjacent genomic sequences. Methods using MEIs as markers for cellular proliferation, as targets for pharmaceuticals, as markers for tissue fingerprinting and in related methods and compositions are disclosed herein. Methods and compositions relate to the detection, treatment and ongoing monitoring of cell proliferation events, cancer, and deleterious effects of mobile elements in aging, and to the selection, use and monitoring of the success of treatment regimens to address these conditions.

Abstract translation: 与使用移动元件插入物及其相邻基因组序列相关的方法和组合物。 使用MEI作为细胞增殖的标志物，作为药物的靶标，作为组织指纹图谱的标记物以及相关方法和组合物的方法在本文中公开。 方法和组成与检测，治疗和持续监测细胞增殖事件，癌症和移动因素在衰老中的有害作用以及治疗方案成功选择，使用和监测以解决这些病症有关。

公开(公告)号：US20160215306A1

公开(公告)日：2016-07-28

申请号：US14915208

申请日：2014-08-15

Applicant: EVOLVA SA

Inventor： Richard Jan Steven Baerends ,  Ernesto Simon ,  Jean Phillippe Meyer ,  Carlos Casado Vazquez

IPC: C12P7/22 ,  C12P19/44 ,  C12N9/10

CPC classification number: C12P7/22 ,  C12N9/1007 ,  C12N9/1051 ,  C12P7/62 ,  C12P19/44 ,  C12Y201/00 ,  C12Y201/01 ,  C12Y204/00

Abstract: Methods for producing glycosylated and methylated resveratrol in a genetically engineered cell, by bioconversion, and in vitro are disclosed herein.

Abstract translation: 本文公开了通过生物转化和体外在遗传工程细胞中产生糖基化和甲基化的白藜芦醇的方法。

公开(公告)号：US20160032257A1

公开(公告)日：2016-02-04

申请号：US14648957

申请日：2013-12-06

Applicant: UNIVERSITÄT HAMBURG

Inventor： Andrea Rentmeister ,  Daniela Stummer

IPC: C12N9/10 ,  C12P19/32

CPC classification number: C12N9/1007 ,  C12P19/32 ,  C12P19/34 ,  C12Y201/01

Abstract: An agent and method for modifying the 5′ cap of RNA, for example for the purposes of isolation and analysis. According to one aspect the invention provides modified enzymes, namely modified trimethylguanosine synthases 2 from Giardia lamblia (GlaTGS2), the enzymatic activity of which is changed such that as compared to wild type enzymes the former can use AdoMet analogues better as cofactors.

Abstract translation: 用于修饰RNA的5'帽的试剂和方法，例如用于分离和分析的目的。 根据一个方面，本发明提供了修饰的酶，即来自贾第鞭毛虫（GlaTGS2）的改性的三甲基鸟苷合酶2，其酶活性被改变，使得与野生型酶相比，前者可以更好地使用AdoMet类似物作为辅因子。

公开(公告)号：US08704040B2

公开(公告)日：2014-04-22

申请号：US12489038

申请日：2009-06-22

Applicant: Joseph Chappell ,  Shigeru Okada ,  Scott Kinison ,  Tom Niehaus

Inventor： Joseph Chappell ,  Shigeru Okada ,  Scott Kinison ,  Tom Niehaus

IPC: C12N15/29 ,  C12N15/52 ,  C12N15/82

CPC classification number: C12N9/1007 ,  C12N15/8243 ,  C12P5/007 ,  C12Y201/01

Abstract: Provided is an isolated polypeptide having triterpene methyltransferase activity. Also provided is an isolated nucleic acid molecule that encodes the triterpene methyltransferase polypeptides; a vector comprising the nucleic acid molecules that encode the triterpene methyltransferase polypeptides; and a host cell(s) transfected with the aforementioned nucleic acid molecule or vector. In another aspect, a method of producing a methylated triterpene is provided. The method comprises providing a metabolizable carbon source to a host cell transfected with a nucleic acid molecule that encodes a triterpene methyltransferase under conditions sufficient for production of a methylated triterpene. The method optionally further comprises isolating the methylated triterpene produced by the host cell.

Abstract translation: 提供了具有三萜甲基转移酶活性的分离的多肽。 还提供了编码三萜甲基转移酶多肽的分离的核酸分子; 包含编码三萜甲基转移酶多肽的核酸分子的载体; 和用上述核酸分子或载体转染的宿主细胞。 另一方面，提供了生产甲基化三萜的方法。 该方法包括向在用于生产甲基化三萜烯的条件下编码三萜甲基转移酶的核酸分子转染的宿主细胞提供可代谢碳源。 所述方法任选进一步包括分离由宿主细胞产生的甲基化三萜烯。

公开(公告)号：US20130247252A1

公开(公告)日：2013-09-19

申请号：US13800930

申请日：2013-03-13

Applicant: THE TEXAS A&M UNIVERSITY SYSTEM

Inventor： Mona B. DAMAJ ,  T. Erik MIRKOV ,  Terry L. THOMAS ,  Keerti S. RATHORE ,  Chandrakanth EMANI ,  Siva P. KUMPATLA

IPC: C12N15/113

CPC classification number: C12N15/113 ,  C07K14/415 ,  C12N9/1007 ,  C12N15/8226 ,  C12Y201/01

Abstract: The present disclosure relates, according to some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant (e.g., a monocot) using a promoter operable in one or more plant tissues and/or cells. In some embodiments, an isolated nucleic acid may comprise an expression control sequence having the sequence of nucleotides 1-4726 of SEQ ID NO: 1, wherein the expression control sequence has stem-regulated and/or defense-inducible promoter activity in at least one monocot (e.g., at least two monocots).

Abstract translation: 根据一些实施方案，本公开涉及使用可在一个或多个植物组织和/或细胞中可操作的启动子在植物（例如，单子叶植物）中表达基因产物的组合物，生物体系，系统和方法。 在一些实施方案中，分离的核酸可以包含具有SEQ ID NO：1的核苷酸1-4726的序列的表达调控序列，其中所述表达控制序列在至少一个中具有干细胞调节和/或防御诱导型启动子活性 单子叶植物（例如，至少两个单子叶植物）。

公开(公告)号：US20120244549A1

公开(公告)日：2012-09-27

申请号：US13429066

申请日：2012-03-23

Applicant: Robert G. Lowery ,  Matt Staeben ,  Tony A. Klink

Inventor： Robert G. Lowery ,  Matt Staeben ,  Tony A. Klink

IPC: G01N21/64

CPC classification number: C12Y201/01 ,  G01N21/6445 ,  G01N33/6815 ,  G01N2333/91011

Abstract: This invention provides methods to determine the activity of methyltransferase enzymes which employ S-adenosylmethionine (SAM) as a substrate and transfer a methyl group to convert this substrate to S-adenosylhomocysteine (SAH), by measuring SAH conversion to AMP.

Abstract translation: 本发明提供了通过测量SAH转化为AMP来测定使用S-腺苷甲硫氨酸（SAM）作为底物并转移甲基以将该底物转化为S-腺苷高半胱氨酸（SAH）的甲基转移酶的活性的方法。

公开(公告)号：US12065668B2

公开(公告)日：2024-08-20

申请号：US17450135

申请日：2021-10-06

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Morgan Maeder

IPC: C12N15/90 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85

CPC classification number: C12N15/907 ,  C07K14/005 ,  C07K14/195 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12Y301/21004 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00

Abstract: Methods and constructs for RNA-guided targeting of heterologous functional domains such as transcriptional activators to specific genomic loci.

公开(公告)号：US20240117394A1

公开(公告)日：2024-04-11

申请号：US17876134

申请日：2022-07-28

Applicant: ATX PHARMERS LLC

Inventor： Vivek PANDYA ,  Tamsen VALOIR

IPC: C12P17/10 ,  C12N1/16 ,  C12N1/20 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52 ,  C12N15/74 ,  C12N15/81

CPC classification number: C12P17/10 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0083 ,  C12N9/1007 ,  C12N9/1205 ,  C12N9/88 ,  C12N15/52 ,  C12N15/74 ,  C12N15/81 ,  C12N2830/002 ,  C12Y114/99 ,  C12Y201/01 ,  C12Y207/01 ,  C12Y401/01

Abstract: Microbes are transformed with psilocybin genes under the control of weak or medium level promoter to make low levels of psilocybin therein. Low dose, microdose and sub-microdose foodstuff are then made with such microbes.