公开(公告)号：US20040094575A1

公开(公告)日：2004-05-20

申请号：US10669796

申请日：2003-09-25

Inventor： Hiroatsu Toi ,  Kenji Yamada ,  Tadashi Ohkawara ,  Masashi Nagaoka

IPC: B67D005/52

CPC classification number: G01N35/1074 ,  G01N35/028 ,  G01N35/109 ,  G01N2035/1076

Abstract: A microplate liquid handling system capable of automatically performing both longitudinal collective suction/discharge and lateral collective suction/discharge on a single microplate. The microplate liquid handling system has a main body equipped with a moving device, a rotating mechanism, a dispensing mechanism, and a stage. Connected to the moving device is the dispensing mechanism for performing dispensing on wells in a microplate on the stage through the rotating mechanism. A plurality of cylinders are arranged in a row in the dispensing mechanism. The dispensing mechanism is capable of moving forwards and backwards and to the right and left with respect to the main body, and is rotatable about a vertically directed rotation axis, making it possible to change the direction of the cylinder array from origin position to 90 degrees position and vice versa.

Abstract translation: 一种微孔板液体处理系统，能够在单个微孔板上自动执行纵向共同吸入/排出和侧向共同吸入/排出。 微板液体处理系统具有配备有移动装置，旋转机构，分配机构和台架的主体。 连接到移动装置的是分配机构，用于通过旋转机构在台上的微板中进行分配。 多个气缸在分配机构中排成一列。 分配机构能够相对于主体向前和向后以及向左右移动，并且可以围绕垂直定向的旋转轴线旋转，使得可以将气缸阵列的方向从原点位置改变到90度 位置，反之亦然。

公开(公告)号：US20040022689A1

公开(公告)日：2004-02-05

申请号：US10630199

申请日：2003-07-30

Applicant: CyBio AG

Inventor： Juergen Wulf ,  Werner Pongratz ,  Andreas Fina ,  Michael Heil ,  Michael Jurtz

IPC: G01N021/00

CPC classification number: G01N35/109 ,  G01N21/6428 ,  G01N21/6452 ,  G01N35/1011 ,  G01N35/1065 ,  G01N2021/6443 ,  G01N2021/6482 ,  G01N2035/0422

Abstract: The invention is directed to a device for dispensing and observing the luminescence of individual specimens in multi-specimen arrangements, particularly for the examination of biological, chemical or cytobiological assays with high specimen throughput. It is the object of the invention to find a novel possibility for dispensing in multi-specimen arrangements (microplates) and for observing the luminescence of the individual specimens, particularly for examining biological assays with high specimen throughput, which permits an immediate observation of the course of luminescence while dispensing proceeds at the same time without being limited to a determined type of microplate. This object is met, according to the invention, in that the dispensing unit has at least one linear dispensing comb containing an even number of dispensing nozzles representing an integral divisor of the number of wells along one dimension of the microplate, the dispensing combs are arranged so as to be displaceable orthogonal to their longitudinal dimension, every dispensing comb is connected to a controllable pump for metering the amount of liquid to be dispensed without immersion in the wells of the microplate, and the CCD camera is oriented by a fast optical system to a large-area rectangular region of the underside of the microplate across from the dispensing unit, the surface being adapted to the dimension of the dispensing comb and to the area of the microplate covered by the dispensing comb, so that the elapsed time for the luminescence is measurable simultaneously while dispensing continues.

Abstract translation: 本发明涉及一种用于分配和观察多个样品装置中的各个样品的发光的装置，特别是用于以高样品生产量检查生物，化学或细胞生物学测定。 本发明的目的是找到一种新型的多样品布置（微孔板）分配和用于观察单个样品的发光的可能性，特别是用于检查具有高样品通量的生物测定法，其允许立即观察该过程 的发光，同时分配在同一时间进行，而不限于确定类型的微孔板。 根据本发明，符合本发明的目的在于，分配单元具有至少一个线性分配梳，其包含偶数个分配喷嘴，分配喷嘴表示沿着微孔板的一个维度的孔数的积分因子，分配梳被布置 为了能够与其纵向尺寸正交地移动，每个分配梳都连接到可控制的泵，用于计量待分配的液体量而不浸没在微板的孔中，并且CCD相机通过快速光学系统定向 微孔板下面的从分配单元的大面积矩形区域，该表面适于分配梳的尺寸和由分配梳覆盖的微板的面积，使得发光的经过时间 可同时测量，同时分配持续。

公开(公告)号：US20040018119A1

公开(公告)日：2004-01-29

申请号：US10622414

申请日：2003-07-18

Inventor： Peter Massaro

IPC: G01N035/00

CPC classification number: B01L3/022 ,  B01J2219/00364 ,  B01L3/0217 ,  B01L2300/049 ,  B01L2300/0829 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0694 ,  C40B60/14 ,  G01N35/1072 ,  G01N35/1074 ,  G01N35/109 ,  Y10T436/2575

Abstract: A robotically manipulable sample handling tool, such as a colony picking head or robotic pipetting tool, includes needles arranged on the tool. Actuators are associated with each needle to control flow for the needle. A plurality of plungers are each associated with a needle, and movement of the plungers can actuate a corresponding needle. Each of the plungers has a passageway that may be opened or closed, e.g., to move the corresponding needle and/or draw fluid into/expel fluid from the needle when the plunger is moved in the tool body. The actuators are arranged so that plunger passageways may be individually controlled by a controller.

Abstract translation: 机器人可操作的样品处理工具，例如菌落采集头或机器人移液工具，包括布置在工具上的针。 执行器与每个针头相关联，以控制针头的流动。 多个柱塞各自与针相关联，并且柱塞的移动可以致动相应的针。 每个柱塞具有可以打开或关闭的通道，例如当活塞在工具主体中移动时，使相应的针和/或将流体从针中移出/排出流体。 致动器被布置成使得柱塞通道可以由控制器单独地控制。

公开(公告)号：US20030215360A1

公开(公告)日：2003-11-20

申请号：US10144763

申请日：2002-05-15

Applicant: Genetix Limited

Inventor： Trevor Ruddock

IPC: G01N035/00

CPC classification number: B01L3/0279 ,  B01L9/543 ,  B01L2200/143 ,  B01L2300/02 ,  B01L2300/0829 ,  G01N35/0099 ,  G01N35/109 ,  G01N2035/00277 ,  G01N2035/103 ,  G01N2035/1076 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165

Abstract: A liquid handling robot for handling well plates. The robot has a powered anvil which loads pipette tips from a tray onto the cones of a liquid handling head by first clamping the liquid handling head to the anvil and then ramming a movable plate carrying the pipette tips onto the cones. The robot also allows for fully automated swapping between different heads, such as liquid handling heads or pin heads. A head parking station is provided for parking heads when they are not being used. The robot has an automated pipette tip tray dispenser based on storage cassettes that store vertically stacked pipette tip trays. The trays are dispensed one at a time out of the storage cassette past retaining catches which are normally inwardly positioned to carry the pipette tip trays, but are outwardly biasable by a dispensing mechanism to allow the lowest tray in the shaft to pass onto a conveyor which moves the tray to a pick-up position from which the head can move the tray to the powered anvil for pipette tip loading onto a liquid handling head.

公开(公告)号：US20030054543A1

公开(公告)日：2003-03-20

申请号：US10286194

申请日：2002-11-01

Inventor： William Michael Lafferty

IPC: C12M001/34 ,  G06K009/00

CPC classification number: G01N21/253 ,  G01N35/109 ,  G01N2035/00158 ,  G01N2035/0494

Abstract: A device for positioning the tip of an elongated probe at a selected station of a holding plate includes motors to move the holding plate and a supporting stage within a coordinate plane (mxy). The elongated probe is also moveable along a probe axis that is oriented normal to the coordinate plane (mxy). A camera creates a pixel image of an optical marker placed on the stage. The image defines a coordinate plane (pxy). To relate the coordinate plane (pxy) to the coordinate plane (mxy), the optical marker is moved to successive locations in the mxy plane and a pixel image is obtained at each location. Using the pixel images, a computer calculates the relationship between coordinate planes and uses the relationship to signal the motors to move the holding plate in the mxy plane and position the selected station on the probe axis for interaction with the probe.

Abstract translation: 用于将细长探针的尖端定位在保持板的选定站处的装置包括将保持板和支撑台移动到坐标平面（mxy）内的马达。 细长探针也可沿着垂直于坐标平面（mxy）定向的探针轴线移动。 相机创建放置在舞台上的光学标记的像素图像。 图像定义坐标平面（pxy）。 为了将坐标平面（pxy）与坐标平面（mxy）相关联，将光学标记移动到mxy平面中的连续位置，并在每个位置获得像素图像。 使用像素图像，计算机计算坐标平面之间的关系，并使用该关系来发信号通知电机将保持板移动到mxy平面中，并将选定的工位放置在探针轴上，以便与探头相互作用。

公开(公告)号：US06511849B1

公开(公告)日：2003-01-28

申请号：US09299193

申请日：1999-04-23

Applicant: Eugenia Wang

Inventor： Eugenia Wang

IPC: G01N3502

CPC classification number: G01N35/109 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00369 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00707 ,  B01J2219/00722 ,  C40B40/06 ,  G01N2035/1034 ,  Y10T436/113332

Abstract: An apparatus and a method are provided for forming a microarray of biological material on a substrate. The microarrays may consist of nucleic acids, peptides, or the like. The subject invention provides relatively simple and inexpensive machinery to produce the microarrays in a reproducible and rapid manner.

Abstract translation: 提供了一种用于在基底上形成生物材料微阵列的装置和方法。 微阵列可以由核酸，肽等组成。 本发明提供了以可再现和快速的方式生产微阵列的相对简单和便宜的机器。

公开(公告)号：US20020176801A1

公开(公告)日：2002-11-28

申请号：US10061416

申请日：2002-02-01

Inventor： Robert H. Giebeler ,  David G. Ogle ,  Roger A. Kaye ,  Steven A. McNerney ,  Gillian M. Humphries ,  Dean G. Hafeman ,  Douglas N. Modlin

IPC: G01N021/00 ,  G01N035/00

CPC classification number: G01N21/253 ,  G01N35/1065 ,  G01N35/109 ,  G01N2035/00326 ,  G01N2035/00356 ,  G01N2035/00455 ,  G01N2035/0498 ,  G01N2035/103

Abstract: An integrated fluid delivery and analysis system and components thereof for preparing and/or analyzing samples. The components may include a transport module, a fluidics module, and an analysis module, among others.

Abstract translation: 用于制备和/或分析样品的集成流体输送和分析系统及其组件。 组件可以包括传输模块，流体模块和分析模块等。

公开(公告)号：US06372183B1

公开(公告)日：2002-04-16

申请号：US08870537

申请日：1997-06-06

Applicant: Michael Anthony Akong ,  Michael Miller Harpold ,  Gonul Velicelebi ,  Paul Brust

Inventor： Michael Anthony Akong ,  Michael Miller Harpold ,  Gonul Velicelebi ,  Paul Brust

IPC: G01N2164

CPC classification number: G01N33/6872 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5058 ,  G01N35/028 ,  G01N35/109 ,  G01N2035/042 ,  G01N2333/705 ,  G01N2500/00 ,  Y10S436/80 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/11 ,  Y10T436/115831

Abstract: Automated measurement apparatus and methods for automated drug screening procedures are provided. The apparatus is capable of initiating and measuring rapid or transient events, such as cell receptor and/or ion channel activity. In particular, the apparatus is capable of aligning with predetermined positions, one or more samples contained in a multi-well container, initiating the reaction with reagent addition and measuring a resultant attribute for a period of time. The apparatus can substantially continuously measure and record data corresponding to the measured attribute before, during and after initiation of the reaction so that a time course of the rapid or transient event may be determined. After the reaction(s) are complete in one or more wells of the multi-well container, the apparatus can align one or more different wells to be assayed with the predetermined position and repeat the cycle until a predetermined number of wells are assayed. Automated drug screening methods employing the apparatus are also provided for screening for compounds which activate, inhibit or potentiate cellular ion channel or receptor activity. The assays include fluorescent indicator-based assays that use viable cells containing effective levels of a fluorescent indicator which is responsive to changes in ion concentration. Activation of the ion channels or receptors is initiated in the automated measurement apparatus by injection into one or more predetermined cell-containing wells of a reagent which is a known or putative activator of the ion channels or receptors of interest. The resulting activity of the ion channels or receptors, which causes changes in ion concentration in the cytoplasm, is determined by measurement of fluorescence intensity changes of the indicator in response to an excitation wavelength.

Abstract translation: 提供了自动化药物筛选程序的自动测量装置和方法。 该装置能够启动和测量快速或瞬时事件，例如细胞受体和/或离子通道活性。 特别地，该装置能够与预定位置对齐，一个或多个样品包含在多孔容器中，开始与试剂添加的反应并测量所得到的属性一段时间。 该装置可以在反应开始之前，期间和之后基本连续地测量和记录对应于测量属性的数据，使得可以确定快速或瞬态事件的时间过程。 在多孔容器的一个或多个孔中的反应完成之后，该装置可以将待测定的一个或多个不同孔与预定位置对准，并重复循环，直到测定预定数量的孔。 还提供了使用该装置的自动药物筛选方法，用于筛选激活，抑制或增强细胞离子通道或受体活性的化合物。 测定包括基于荧光指示剂的测定法，其使用含有有效水平的荧光指示剂的活细胞，其对离子浓度的变化有反应。 离子通道或受体的激活在自动化测量装置中通过注射到一个或多个预定的含有细胞的孔中，该试剂是已知或推定的感兴趣的离子通道或受体的激活剂。 通过测量响应于激发波长的指示剂的荧光强度变化来确定导致细胞质中离子浓度变化的离子通道或受体的所得活性。

公开(公告)号：US06234033B1

公开(公告)日：2001-05-22

申请号：US09301578

申请日：1999-04-29

Applicant: Heinz Eipel

Inventor： Heinz Eipel

IPC: G01N3510

CPC classification number: G01N35/1011 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00364 ,  B01J2219/00691 ,  C40B60/14 ,  G01N35/109 ,  G01N2035/0491

Abstract: The invention relates to an automatic pipetting apparatus, having a drive for moving a pipetting instrument from a source site to a target site, in which apparatus end stops (8-10, 14, 15), which can be finely adjusted automatically, for the movements of the pipetting instrument (1) generated by the drive are provided at the source site and/or the target site.

Abstract translation: 本发明涉及一种自动移液装置，其具有用于将移液器从源部位移动到目标部位的驱动装置，其中装置端部停止（8-10,14,15），其可以自动精细调节，用于 由驱动器产生的移液器（1）的动作设置在源部位和/或目标部位。

公开(公告)号：US5939326A

公开(公告)日：1999-08-17

申请号：US816712

申请日：1997-03-13

Applicant: Vernon L. Chupp ,  Peter E. Lobban ,  Young Ran Kim ,  Roderick Walton Larue ,  John Paul Stuart

Inventor： Vernon L. Chupp ,  Peter E. Lobban ,  Young Ran Kim ,  Roderick Walton Larue ,  John Paul Stuart

IPC: B01F5/04 ,  G01N1/00 ,  G01N15/00 ,  G01N15/14 ,  G01N35/00 ,  G01N35/10 ,  G01N33/533

CPC classification number: G01N35/109 ,  B01F5/0453 ,  B01F5/046 ,  G01N15/14 ,  G01N15/1404 ,  G01N35/0092 ,  G01N35/1004 ,  G01N35/1095 ,  G01N1/14 ,  G01N2015/0069 ,  G01N2015/0076 ,  G01N2015/0084 ,  G01N2015/1413 ,  G01N2035/00356 ,  G01N2035/00524 ,  G01N2035/1032 ,  G01N35/10 ,  Y10S436/805 ,  Y10T436/11 ,  Y10T436/119163 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2575

Abstract: A device for analyzing a whole blood sample is provided. The device comprises a conventionl hematology analyzer integrated with a fluorescence cyometry analyzer. A controller is provided for controlling the analyzers, obtaining and utilizing data from both and reporting a quantitative result. Methods are also provided for analyzing a whole blood sample. One such method comprises the steps of performing on a single instrument an analysis of impedance associated with the blood sample, an analysis of light scatter associated with the blood sample, and an analysis of fluorescence associated with the blood sample. Data is collected and utilized. A result is reported.

Abstract translation: 提供了用于分析全血样品的装置。 该装置包括与荧光测定分析仪集成的常规血液分析仪。 提供控制器用于控制分析器，获得和利用来自两者的数据并报告定量结果。 还提供了用于分析全血样品的方法。 一种这样的方法包括以下步骤：在单个仪器上执行与血液样品相关的阻抗的分析，与血液样品相关联的光散射的分析以及与血液样品相关联的荧光的分析。 收集和利用数据。 结果报告。