公开(公告)号：US20150053562A1

公开(公告)日：2015-02-26

申请号：US14526928

申请日：2014-10-29

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

IPC: G01N27/447

CPC classification number: G01N27/447 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  C12Q1/6806 ,  G01N15/1031 ,  G01N27/44717 ,  G01N27/44721 ,  G01N33/543 ,  G01N35/10 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1081 ,  G01N2035/1034 ,  Y10T436/11 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.

Abstract translation: 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。 还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。

公开(公告)号：US20140302580A1

公开(公告)日：2014-10-09

申请号：US14308110

申请日：2014-06-18

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N1/38

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.

Abstract translation: 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在大气场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。

公开(公告)号：US20140231259A1

公开(公告)日：2014-08-21

申请号：US14253556

申请日：2014-04-15

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

IPC: G01N27/447

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0448 ,  B03C5/02 ,  B81B1/00 ,  G01N27/447 ,  G01N2035/1046

Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator including an opaque filler fluid and a transparent droplet thereon is provided. The droplet microactuator may further include a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate; a second substrate spaced apart from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and second substrate; and wherein the droplet and the filler fluid are in the interior volume, and wherein the droplet is surrounded by the filler fluid and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes.

Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了包括不透明填充流体和其上的透明液滴的液滴微致动器。 液滴微致动器还可以包括第一衬底，其包括被配置为在第一衬底的表面上实现电润湿介导的液滴操作的电极; 第二基板，与第一基板的表面间隔开足以限定第一基板和第二基板之间的内部空间的距离; 并且其中所述液滴和所述填充流体在所述内部容积中，并且其中所述液滴被所述填充流体包围并且以允许使用所述电极对所述液滴进行电润湿介导的液滴操作的方式相对于所述电极布置。

公开(公告)号：US10139403B2

公开(公告)日：2018-11-27

申请号：US15266693

申请日：2016-09-15

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Ramakrishna Sista ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: B01L3/00 ,  G01N1/00 ,  G01N1/10 ,  G01N15/06 ,  G01N1/18 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  G01N1/38 ,  B01F13/08 ,  B01L7/00 ,  G01N35/10

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US09574220B2

公开(公告)日：2017-02-21

申请号：US14644989

申请日：2015-03-11

Applicant: Advanced Liquid Logic, Inc.

Inventor： Allen E Eckhardt ,  Carrie Graham ,  Ramakrishna Sista ,  Vamsee K. Pamula ,  Theodore Winger ,  Tong Wang ,  Yalin Xiong ,  Ning Wu ,  Sanjay Saha ,  Gajendrasinh Raolji ,  Raveendra Dayam

IPC: C12Q1/54 ,  C12Q1/34 ,  C07D311/18 ,  C07D311/20 ,  C07H15/203 ,  C12Q1/25 ,  G01N33/543 ,  G01N21/64 ,  C07D311/16 ,  C07D405/12 ,  C07D413/12 ,  C07H17/075 ,  C12Q1/44 ,  B01L3/00

CPC classification number: C12Q1/40 ,  B01L3/502784 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07D311/16 ,  C07D311/18 ,  C07D311/20 ,  C07D405/12 ,  C07D413/12 ,  C07H15/203 ,  C07H17/075 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q1/54 ,  C12Y302/01023 ,  G01N21/6452 ,  G01N33/5438 ,  G01N2333/916 ,  G01N2333/926 ,  G01N2333/938 ,  G01N2800/04

Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.

Abstract translation: 本发明涉及液滴致动器装置和测定方法。 本发明包括进行测定的测定方法，其包括将样品与伞形酮基衍生物组合，其中所述样品潜在地包含能够切割伞形变体基衍生物的酶，并且其中伞形载体衍生物包含用一个或多个修饰部分修饰的伞形酮核心。

公开(公告)号：US20160318021A1

公开(公告)日：2016-11-03

申请号：US15206860

申请日：2016-07-11

Applicant: Advanced Liquid Logic Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Philip Paik

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/0241 ,  B01L3/50273 ,  B01L2200/06 ,  B01L2300/06 ,  B01L2400/0427 ,  B41J2/14

Abstract: A method of manipulating a droplet comprising providing a substrate comprising a surface; an elongated transport electrode disposed on the substrate surface, the elongated transport electrode having a first and a second end and configured to impart a gradient force to the droplet; and one or more wires for providing power to the transport electrode; and providing power to the one or more wires to effect the gradient force and thereby transport the droplet along the length of the elongated transport electrode from the first end to the second end.

公开(公告)号：US09377455B2

公开(公告)日：2016-06-28

申请号：US14746276

申请日：2015-06-22

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  G01N1/38 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US20160016172A1

公开(公告)日：2016-01-21

申请号：US14837133

申请日：2015-08-27

Applicant: ADVANCED LIQUID LOGIC, INC. ,  DUKE UNIVERSITY

Inventor： Michael G. Pollack ,  Philip Paik ,  Vamsee K. Pamula

IPC: B01L7/00 ,  G01N27/447

CPC classification number: C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

Abstract: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.

Abstract translation: 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由其控制的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。

公开(公告)号：US09139865B2

公开(公告)日：2015-09-22

申请号：US13680979

申请日：2012-11-19

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Michael G. Pollack ,  Philip Paik ,  Vamsee K. Pamula

IPC: G05D5/06 ,  C12Q1/68 ,  G01N27/447

CPC classification number: C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

Abstract: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.

Abstract translation: 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由控制器执行指令的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。

公开(公告)号：US20150174578A1

公开(公告)日：2015-06-25

申请号：US14590470

申请日：2015-01-06

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Zishan Hua ,  Arjun Sudarsan ,  Philip Y. Paik

IPC: B01L3/00

CPC classification number: B01L3/52 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/06 ,  B01L2400/0688 ,  F04B19/006

Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.

Abstract translation: 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。