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154 条记录 (耗时 0.039 秒)

    Polynucleotides allowing the expression and secretion of recombinant pseudo-virus containing foreign epitopes, their production, and use
    61.
    发明授权
    Polynucleotides allowing the expression and secretion of recombinant pseudo-virus containing foreign epitopes, their production, and use 有权
    允许含有外源表位的重组假病毒的表达和分泌的多核苷酸，其生产和用途

    公开(公告)号：US09096868B2

    公开(公告)日：2015-08-04

    申请号：US12678046

    申请日：2008-09-12

    申请人： Qiang Deng ,  Marie-Louise Michel

    发明人： Qiang Deng ,  Marie-Louise Michel

    IPC分类号： C12N15/86 ,  C07K14/005 ,  A61K39/00

    CPC分类号： C12N15/86 ,  A61K2039/57 ,  C07K14/005 ,  C07K2319/00 ,  C12N2730/10122

    摘要： This invention provides a new approach to the design of a virus with a defective replication cycle, which can be rescued by wild type virus co-infection, and which expresses foreign antigenic epitopes that contribute to the elimination of virus infected cells and then to viral clearance. The vector of the invention, by expression of epitopes derived from common pathogens, by-passes existing tolerance of virus specific T cell responses. The vector will only replicate in virus infected cells.

    摘要翻译： 本发明提供了一种设计具有缺陷复制周期的病毒的新方法，其可以被野生型病毒共感染拯救，并且其表达有助于消除病毒感染的细胞然后进行病毒清除的外来抗原表位 。 本发明的载体通过表达来自常见病原体的表位，绕过病毒特异性T细胞应答的现有耐受性。 载体只会在病毒感染的细胞中复制。

    Therapy of cancer based on targeting adaptive, innate and/or regulatory component of the immune response
    62.
    发明授权
    Therapy of cancer based on targeting adaptive, innate and/or regulatory component of the immune response 有权
    基于针对免疫应答的适应性，先天和/或调节组分的癌症治疗

    公开(公告)号：US09095537B2

    公开(公告)日：2015-08-04

    申请号：US11778267

    申请日：2007-07-16

    申请人： Claude Leclerc ,  Pedro Berraondo Lopez

    发明人： Claude Leclerc ,  Pedro Berraondo Lopez

    IPC分类号： A61K39/10 ,  A61K39/00 ,  A61K39/12 ,  C07K14/005 ,  C12N7/00 ,  C12N9/88

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/0011 ,  A61K39/12 ,  A61K2039/555 ,  A61K2039/55511 ,  A61K2039/55516 ,  A61K2039/55555 ,  A61K2039/55561 ,  A61K2039/572 ,  A61K2039/585 ,  A61K2039/70 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/33 ,  C07K2319/40 ,  C12N7/00 ,  C12N9/88 ,  C12N2710/20022 ,  C12N2710/20034 ,  C12N2710/20071 ,  C12Y406/01001

    摘要： The invention relates to a kit of parts, suitable for use in a therapy of cancer, wherein said kit comprises:(i) a recombinant protein comprising one or several polypeptides bearing one or several epitopes of one or several tumor-associated antigens, said polypeptides being inserted in the same or different permissive sites of an adenylate cyclase (CyaA) protein or of a fragment thereof, wherein said CyaA fragment retains the property of said adenylate cyclase protein to target Antigen Presenting Cells or a mixture of such recombinant proteins wherein at least one of said epitopes, or tumor associated antigens, or insertion sites of CyaA protein, or fragment of said CyaA protein is different between the various recombinant proteins in the mixture; and said kit of parts further comprises at least one of the following compounds; (ii) an agent, suitable for modulating a regulatory immune response in a patient ad optionally; (iii) an adjuvant component suitable for activating the innate immune response in a patient.

    摘要翻译： 本发明涉及一种适合用于癌症治疗的试剂盒，其中所述试剂盒包括：（i）包含一个或几个携带一个或几个肿瘤相关抗原表位的多肽的重组蛋白，所述多肽 插入腺苷酸环化酶（CyaA）蛋白或其片段的相同或不同的允许位点，其中所述CyaA片段保留所述腺苷酸环化酶蛋白质的特性以靶向抗原呈递细胞或其重要蛋白质的混合物，其中至少 所述表位之一或肿瘤相关抗原或CyaA蛋白的插入位点或所述CyaA蛋白的片段在混合物中的各种重组蛋白之间是不同的; 并且所述试剂盒的部分还包含至少一种以下化合物; （ii）任选的适于调节患者的调节免疫应答的药剂; （iii）适合于激活患者中先天免疫应答的辅助成分。

    Vector comprising multiple homologous nucleotide sequences
    64.
    发明授权
    Vector comprising multiple homologous nucleotide sequences 有权
    包含多个同源核苷酸序列的载体

    公开(公告)号：US08999637B2

    公开(公告)日：2015-04-07

    申请号：US13123605

    申请日：2009-11-20

    申请人： Robin Steigerwald

    发明人： Robin Steigerwald

    IPC分类号： C12Q1/68 ,  C12N15/00 ,  A61K39/12 ,  A61K39/155 ,  C12N15/86

    CPC分类号： C12N15/8636 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/11022 ,  C12N2760/14122 ,  C12N2799/02 ,  C12N2800/22

    摘要： The invention relates to vectors comprising two or more homologous nucleotide sequences and methods for generating them. The invention concerns substituting bases in the homologous nucleotide sequences with different bases that do not alter the encoded amino acid sequence. The invention allows for the reduction of intramolecular recombination between homologous nucleotide sequences, in particular in mammalian cells. The invention further relates to nucleotide sequences containing substituted bases.

    摘要翻译： 本发明涉及包含两个或多个同源核苷酸序列的载体和用于产生它们的方法。 本发明涉及用不改变编码氨基酸序列的不同碱基取代同源核苷酸序列中的碱基。 本发明允许减少同源核苷酸序列之间的分子内重组，特别是在哺乳动物细胞中。 本发明还涉及含有取代碱基的核苷酸序列。

    Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
    65.
    发明授权
    Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions 有权
    在无血清条件下培养原代细胞和扩增病毒的方法

    公开(公告)号：US08673318B2

    公开(公告)日：2014-03-18

    申请号：US13688473

    申请日：2012-11-29

    申请人： Bavarian Nordic A/S

    发明人： Ingmar Rathe ,  Eva Felder ,  Karl Heller

    IPC分类号： A61K39/275 ,  C12P1/00 ,  C12N7/00 ,  C12N7/02 ,  C12N5/00 ,  C12N5/07

    CPC分类号： C12N7/08 ,  C12N5/0606 ,  C12N5/0652 ,  C12N7/00 ,  C12N2500/24 ,  C12N2501/11 ,  C12N2510/02 ,  C12N2710/24151

    摘要： The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.

    摘要翻译： 本发明涉及一种培养原代细胞的方法。 将原代细胞培养在包含选自生长因子和附着因子的因子的无血清培养基中。 用于培养原代细胞的方法可以是用于扩增病毒如痘病毒的方法的一个步骤。 根据后一种方法，将原代细胞培养在包含选自生长因子和附着因子的因子的无血清培养基中。 然后用病毒感染细胞，将感染的细胞在无血清培养基中培养，直到产生子代病毒。

    Cells and methodology to generate non-segmented negative-strand RNA viruses
    67.
    发明授权
    Cells and methodology to generate non-segmented negative-strand RNA viruses 有权
    产生非分段负链RNA病毒的细胞和方法

    公开(公告)号：US08586364B2

    公开(公告)日：2013-11-19

    申请号：US12448468

    申请日：2007-12-21

    申请人： Frédéric Tangy ,  Pierre Charneau ,  Yves Jacob

    发明人： Frédéric Tangy ,  Pierre Charneau ,  Yves Jacob

    IPC分类号： C12N7/01 ,  C12N7/02 ,  C12N7/04 ,  A61K39/165

    CPC分类号： C12N7/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/16021 ,  C12N2740/16043 ,  C12N2740/16052 ,  C12N2760/18022 ,  C12N2760/18422 ,  C12N2830/48

    摘要： The present invention relates to recombinant cells as well as to methods for the generation of non-segmented negative-sense single-stranded RNA viruses (NNV or mononegavirales) from cloned deoxyribonucleic acid (cDNA), especially from measles virus and in particular from attenuated strains such as those approved for vaccination, in particular from the attenuated Schwarz measles virus and various recombinant Schwarz measles-based viruses expressing heterologous sequences. Such rescued viruses can be used, after amplification, as vaccines for immunization against measles and/or against the heterologous peptides or proteins expressed.

    摘要翻译： 本发明涉及重组细胞，以及用于从克隆的脱氧核糖核酸（cDNA），特别是麻疹病毒，特别是从减毒菌株中产生非分段的负义单链RNA病毒（NNV或单酮巴西）的方法 例如批准用于疫苗接种的那些，特别是来自减毒的施瓦氏麻疹病毒和表达异源序列的各种重组Schwarz麻疹病毒。 这样的拯救病毒可以在扩增后用作针对麻疹免疫的疫苗和/或针对所表达的异源肽或蛋白质。

    Fusion proteins of HIV regulatory/accessory proteins
    70.
    发明授权
    Fusion proteins of HIV regulatory/accessory proteins 有权
    HIV调节/辅助蛋白的融合蛋白

    公开(公告)号：US08435535B2

    公开(公告)日：2013-05-07

    申请号：US13372723

    申请日：2012-02-14

    申请人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

    发明人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

    IPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  C12P1/00 ,  C12P21/04 ,  C12N5/00

    CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

    摘要： The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections.

    摘要翻译： 本发明涉及包含选自Vif，Vpr，Vpu，Rev和Tat的至少三种HIV蛋白质的氨基酸序列或一种或多种所述蛋白质的氨基酸序列的衍生物的融合蛋白，其中融合蛋白不是 加工成具有天然N和C末端的单独的HIV蛋白质。 本发明还涉及编码所述蛋白质的核酸，包含所述核酸的载体和用于产生所述蛋白质的方法。 融合蛋白，核酸和载体可用作用于至少部分预防HIV感染的疫苗。