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公开(公告)号：US20160091479A1
公开(公告)日：2016-03-31
申请号：US14809972
申请日：2015-07-27
Applicant: Fluidigm Corporation
Inventor： Hany Nassef , Geoffrey Facer , Marc Unger
IPC: G01N33/487 , G01N15/12 , G01N15/10
CPC classification number: C03C25/68 , B01L3/502707 , B01L2300/0645 , B01L2300/0816 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , G01N15/1031 , G01N15/12 , G01N27/00 , G01N27/60 , G01N33/48707 , G01N33/48721 , G01N2015/1087 , Y10T436/2575
Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.
Abstract translation: 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。
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公开(公告)号：US09284605B2
公开(公告)日：2016-03-15
申请号：US14318104
申请日：2014-06-27
Applicant: Fluidigm Corporation
Inventor： Stanley N. Lapidus
IPC: C12Q1/68
CPC classification number: C12Q1/68 , C12Q1/6869 , C12Q1/6874 , C12Q2565/518 , C12Q2563/107 , C12Q2533/101
Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.
Abstract translation: 本发明提供了使用固定的捕获探针和单分子测序技术来检测，列举或鉴定靶核酸分子的方法和装置。
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公开(公告)号：US09234237B2
公开(公告)日：2016-01-12
申请号：US14547442
申请日：2014-11-19
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
IPC: G01N21/00 , G01N15/06 , C12Q1/68 , G01N21/64 , G01N27/447 , G02B21/16 , G01N21/84 , G01N21/75 , G02B21/36 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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74.发明授权公开(公告)号：US09182322B2
公开(公告)日：2015-11-10
申请号：US14091344
申请日：2013-11-27
Applicant: Fluidigm Corporation
Inventor： Tim Woudenberg , Jing Wang , Hou-Pu Chou
CPC classification number: B01L3/502715 , B01F13/0059 , B01F13/0094 , B01F13/1013 , B01F13/1022 , B01J2219/00317 , B01J2219/00479 , B01J2219/00585 , B01L3/5025 , B01L3/502723 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/027 , B01L2200/0605 , B01L2300/0829 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , B01L2400/082 , B33Y80/00 , G01N1/28 , H04W4/70 , H04W76/30
Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.
Abstract translation: 描述了包括刚性基层和基层上的弹性体层的微流体装置。 弹性体层可以包括用于输送液体试剂的流体通道的至少一部分，以及排气通道，其接纳从流动通道穿过弹性体层扩散的气体并将其从弹性体层排出。 装置还可以包括流体连接到流体通道的混合室，以及与限定流动通道的一部分的可偏转膜重叠的控制通道，其中控制通道可操作以改变液体试剂流动的速率 通过流体通道。 所述装置还可包括弹性体层上的刚性塑料层。
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公开(公告)号：US20150159132A1
公开(公告)日：2015-06-11
申请号：US14568597
申请日：2014-12-12
Applicant: Fluidigm Corporation
Inventor： NIANZHEN LI , Marc Unger
CPC classification number: C12N5/0618 , B01L3/5027 , B01L2200/0647 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , C12M23/16 , C12N5/0619 , C12N15/111 , C12N2310/141 , C12N2320/12 , C12N2501/65 , C12N2503/02 , C12N2506/02 , C12N2506/1307 , C12Q1/6881 , C12Q2600/158 , G01N33/5026 , G01N33/57438
Abstract: This invention provides technology for transdifferentiating cells from one cell type to another. The cells are cultured with one or more vector-free gene regulator oligonucleotides concurrently or in succession, and then harvested when cell markers or the morphology of the culture shows that transdifferentiation is complete. Suitable gene regular oligonucleotides include microRNAs and messenger RNAs that encode a differentiation factor. Conditions for transdifferentiation can be optimized by dividing cells into different culture chambers of a microfluidic device. Cells are cultured with different additives in each chamber, and then compared. Transdifferentiated cells produced according to this invention can provide a consistent source of tissue for use in regenerative medicine.
Abstract translation: 本发明提供了将细胞从一种细胞类型转移到另一种细胞类型的技术。 细胞与一种或多种无载体的基因调节寡核苷酸同时或相继培养，然后当细胞标记物或培养物的形态显示转分化完成时收获细胞。 合适的基因正常寡核苷酸包括编码分化因子的微小RNA和信使RNA。 可以通过将细胞分成微流体装置的不同培养室来优化用于转分化的条件。 每个室中用不同的添加剂培养细胞，然后进行比较。 根据本发明生产的转分化细胞可提供用于再生医学的一致的组织来源。
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Patent Agency Ranking
公开(公告)号：US08926905B2
公开(公告)日：2015-01-06
申请号：US13937340
申请日：2013-07-09
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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公开(公告)号：US20140296090A1
公开(公告)日：2014-10-02
申请号：US14184499
申请日：2014-02-19
Applicant: FLUIDIGM CORPORATION
Inventor： Alain Mir , Ramesh Ramakrishnan , Marc Unger , Bernhard G. Zimmermann
Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay.
Abstract translation: 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。
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公开(公告)号：US20140154679A1
公开(公告)日：2014-06-05
申请号：US13869804
申请日：2013-04-24
Applicant: Fluidigm Corporation
Inventor： Simant Dube , Alain Mir , Ramesh Ramakrishnan , Lesley Suzanne Weaver , Bernhard G. Zimmermann
IPC: C12Q1/68
CPC classification number: C12Q1/6853 , C12Q1/6809 , C12Q1/6886 , C12Q2600/16 , C12Q2561/113 , C12Q2545/10 , C12Q2527/107
Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.
Abstract translation: 本发明提供了确定测试样品和参考样品之间的一个或多个目标核酸序列的相对拷贝数差异或其衍生的参考值。 该方法有助于检测小于1.5倍的拷贝数差异。
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公开(公告)号：US08685326B2
公开(公告)日：2014-04-01
申请号：US13937340
申请日：2013-07-09
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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80.发明申请公开(公告)号：US20130302884A1
公开(公告)日：2013-11-14
申请号：US13781328
申请日：2013-02-28
Applicant: FLUIDIGM CORPORATION
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A.A. West
IPC: G01N1/28
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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