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公开(公告)号：US20100236927A1
公开(公告)日：2010-09-23
申请号：US12681840
申请日：2008-10-17
IPC分类号： G01N27/26
CPC分类号： B01L3/502792 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/161 , B01L2400/0427 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： A droplet actuator comprising a substrate comprising an electrode coupled to a voltage source, wherein the droplet actuator is configured such that when voltage is applied to the electrode, an electrostatic energy gradient is established at a surface of the substrate which causes a droplet to be transported in a direction established by the energy gradient. Related methods and other embodiments are also provided.
摘要翻译： 一种液滴致动器，包括包括耦合到电压源的电极的基板，其中所述液滴致动器被配置为使得当电压被施加到所述电极时，在所述基板的表面处建立静电能量梯度，这导致液滴被输送 在能量梯度建立的方向上。 还提供了相关方法和其它实施例。
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公开(公告)号：US07759132B2
公开(公告)日：2010-07-20
申请号：US11585491
申请日：2006-10-23
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Methods for performing microfluidic sampling are provided. The method includes providing a substrate including an arrangement of first, second and third electrodes, wherein the second electrode is interposed between the first and third electrodes. The method additionally includes causing a fluid input to continuously flow to the first electrode and biasing the first, second and third electrodes to a first voltage to cause a portion of the fluid input to spread across the second and third electrodes. The method further includes biasing the second electrode to a second voltage different from the first voltage to form a droplet on the third electrode, the droplet being separate from the fluid input.
摘要翻译： 提供了执行微流体取样的方法。 该方法包括提供包括第一，第二和第三电极的布置的基板，其中第二电极介于第一和第三电极之间。 该方法还包括使流体输入连续地流动到第一电极并且将第一，第二和第三电极偏压到第一电压以使流体输入的一部分扩展穿过第二和第三电极。 该方法还包括将第二电极偏压到不同于第一电压的第二电压，以在第三电极上形成液滴，液滴与流体输入分离。
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73.发明授权公开(公告)号：US06911132B2
公开(公告)日：2005-06-28
申请号：US10253368
申请日：2002-09-24
IPC分类号： B01F11/00 , B01F13/00 , B01L3/00 , G01N1/00 , G01N1/10 , G01N15/06 , G01N21/00 , G01N35/00 , G01N27/26 , B01D57/00
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： An apparatus is provided for manipulating droplets. The apparatus is a single-sided electrode design in which all conductive elements are contained on one surface on which droplets are manipulated. An additional surface can be provided parallel with the first surface for the purpose of containing the droplets to be manipulated. Droplets are manipulated by performing electrowetting-based techniques in which electrodes contained on or embedded in the first surface are sequentially energized and de-energized in a controlled manner. The apparatus enables a number of droplet manipulation processes, including merging and mixing two droplets together, splitting a droplet into two or more droplets, sampling a continuous liquid flow by forming from the flow individually controllable droplets, and iterative binary or digital mixing of droplets to obtain a desired mixing ratio.
摘要翻译： 提供了一种用于操纵液滴的装置。 该装置是单面电极设计，其中所有导电元件都包含在其上操作液滴的一个表面上。 为了容纳待操作的液滴，可以与第一表面平行地设置另外的表面。 通过执行基于电润湿的技术来操纵液滴，其中包含在或嵌入在第一表面中的电极被顺序地通电并以受控的方式断电。 该装置能够进行多个液滴操纵过程，包括将两个液滴合并和混合在一起，将液滴分成两个或更多个液滴，通过从流动单独控制的液滴形成连续的液体流，以及液滴的迭代二进制或数字混合到 获得所需的混合比。
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公开(公告)号：US08716015B2
公开(公告)日：2014-05-06
申请号：US12334575
申请日：2008-12-15
CPC分类号： C12N13/00 , B01J2219/00527 , B01J2219/00653 , B01J2219/00743 , G01N15/10 , G01N35/10 , G01N2015/0088 , G01N2015/1081 , G01N2035/00564 , G01N2035/1034 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： A method of inoculating a culture medium including providing a droplet including a single cell type on a droplet actuator and inoculating a culture medium with the droplet. A method of providing a metabolically useful substance to a cell culture, including providing a droplet actuator including a cell culture droplet loaded thereon, the sample droplet including cells and a cell culture medium, and a second droplet comprising a metabolically useful substance. The method also includes conducting one or more droplet operations to combine the cell culture droplet with the second droplet on the droplet actuator. Related methods, droplet actuators, and systems are also provided.
摘要翻译： 一种接种培养基的方法，包括在液滴致动器上提供包含单细胞类型的液滴并用液滴接种培养基。 提供代谢有用的物质给细胞培养物的方法，包括提供包括其上装载有细胞培养物液滴的液滴致动器，所述样品液滴包括细胞和细胞培养基，以及包含代谢有用物质的第二液滴。 该方法还包括进行一个或多个液滴操作以将细胞培养液滴与液滴致动器上的第二液滴组合。 还提供了相关方法，液滴致动器和系统。
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75.发明授权公开(公告)号：US08349276B2
公开(公告)日：2013-01-08
申请号：US11343284
申请日：2006-01-30
IPC分类号： B01L3/00
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets on a printed circuit board (PCB) are disclosed. Droplets are actuated upon a printed circuit board substrate surface by the application of electrical potentials to electrodes defined on the PCB. The use of soldermask as an electrode insulator for droplet manipulation as well techniques for adapting other traditional PCB layers and materials for droplet-based microfluidics are also disclosed.
摘要翻译： 公开了在印刷电路板（PCB）上操纵液滴的装置和方法。 通过将电位施加到PCB上限定的电极，使液滴在印刷电路板基板表面上被致动。 还公开了使用焊接掩模作为液滴操作的电极绝缘体以及用于适应其它传统PCB层和用于液滴的微流体的材料的技术。
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公开(公告)号：US08221605B2
公开(公告)日：2012-07-17
申请号：US11965152
申请日：2007-12-27
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： An apparatus for manipulating droplets is provided. In one embodiment, the apparatus includes a substrate having electrodes and adjacent reference elements configured for manipulating a droplet on a surface of the substrate. Other embodiments having a top plate, a footprint, and a dielectric are also included.
摘要翻译： 提供了一种用于操纵液滴的装置。 在一个实施例中，该装置包括具有电极和相邻参考元件的基板，该参考元件被配置用于在基板的表面上操纵液滴。 还包括具有顶板，覆盖区和电介质的其它实施例。
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公开(公告)号：US20100323405A1
公开(公告)日：2010-12-23
申请号：US12665788
申请日：2008-06-23
IPC分类号： C12P19/34
CPC分类号： C12Q1/6846 , C12Q2527/15 , C12Q2527/101
摘要： A method of amplifying a nucleic acid, the method comprising cycling an amplification-ready droplet through a temperature gradient to locations within the temperature gradient suitable for effecting steps in an amplification reaction.
摘要翻译： 一种扩增核酸的方法，所述方法包括使扩增准备的液滴通过温度梯度循环到适于在扩增反应中进行步骤的温度梯度内的位置。
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公开(公告)号：US20100291578A1
公开(公告)日：2010-11-18
申请号：US12789891
申请日：2010-05-28
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.
摘要翻译： 本发明涉及基于液滴的焦磷酸测序，包括鉴定样品核酸中目标位置的碱基的方法。 该方法包括：（a）提供包括包含固定在珠粒上的样品核酸的第一液滴的液滴微致动器; 和（b）在液滴微致动器上：（i）使第一液滴与一个或多个试剂液滴接触以产生第二液滴，其中所述一个或多个试剂液滴包括用于通过掺入来扩展样品核酸的双链部分的试剂 目标位置的核苷酸; （ii）分裂第二液滴以产生包含珠粒的第三液滴和不含珠粒的第四液滴; 和（iii）测定第三液滴以确定核苷酸是否并入目标位置。
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公开(公告)号：US07816121B2
公开(公告)日：2010-10-19
申请号：US11639990
申请日：2006-12-15
CPC分类号： B01L3/502761 , B01L3/5025 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/147 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448 , B01L2400/0487 , G01N15/1484 , G01N2015/1006 , G01N2035/1046 , Y10T29/49002 , Y10T137/0318
摘要： A droplet actuation system is provided including a droplet actuation device including a substrate that includes electrodes for conducting droplet operations; temperature control means for heating and/or cooling a region of the droplet actuation device and arranged such that a droplet can be transported on the electrodes to a region for heating; and a means for effecting a magnetic field in proximity to one or more of the electrodes sufficient to immobilize magnetically responsive beads in a droplet on the substrate during droplet operations. The system further includes a processor for controlling the electrodes and temperature control means, wherein the processor is programmed, and electrodes and magnetic field are arranged, to cause the electrodes to split a droplet including the magnetically responsive beads yielding a first daughter droplet which includes the magnetically responsive beads and a second daughter droplet without a substantial amount of the magnetically responsive beads.
摘要翻译： 提供了一种液滴致动系统，其包括液滴致动装置，其包括包括用于进行液滴操作的电极的基板; 温度控制装置，用于加热和/或冷却液滴致动装置的区域，并布置成使得液滴可以在电极上传送到加热区域; 以及用于在一些或多个电极处进行磁场的装置，其足以在液滴操作期间将磁响应珠固定在基板上的液滴中。 该系统还包括用于控制电极和温度控制装置的处理器，其中处理器被编程，并且布置电极和磁场，以使电极分裂包括磁响应珠的液滴，产生包含第一子液滴的第一子液滴 磁响应珠和第二子液滴，而没有大量的磁响应珠。
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公开(公告)号：US20090280475A1
公开(公告)日：2009-11-12
申请号：US11639710
申请日：2006-12-15
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting sequencing protocols, such as the reagents for conducting a pyrosequencing protocol. The invention also includes a droplet microactuator or droplet microactuator system, having one or more input reservoirs loaded with a sample for conducting a pyrosequencing protocol.
摘要翻译： 液滴微致动器技术领域本发明涉及液滴微致动器以及采用液滴微致动器来使用液滴执行各种协议的系统，装置和方法。 本发明包括液滴微致动器或液滴微致动器系统，其具有一个或多个输入储存器，其中装载有用于进行测序方案的试剂，例如用于进行焦磷酸测序方案的试剂。 本发明还包括液滴微致动器或液滴微致动器系统，其具有装载有用于进行焦磷酸测序方案的样品的一个或多个输入储存器。
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