摘要:
Provided is an analyzing apparatus which can improve an efficiency of detecting light generated from reaction chambers in a microfluidic disk and reduce the occurrence of crosstalk between neighboring reaction chambers. The analyzing apparatus includes: a rotatable disk in which a plurality of reaction chambers where samples and reagents react with each other are formed; a rotation driver rotating the disk; and a photodetector unit detecting light generated from the reaction chambers, and the photodetector unit comprises: a photodetecting device receiving the light to generate electrical signals; and a light transmitting element receiving the light generated from the reaction chambers and transferring the received light to the photodetecting device.
摘要:
A reaction of a sample mixture liquid is detected accurately, by allowing a solid reagent to be dissolved and reacted in a liquid sample quickly and accurately. A disc main body, and at least one sample mixing unit provided at the disc main body are provided, and the sample mixing unit is provided with: a liquid sample reserve unit; a reagent chamber capable of disposing a plurality of the solid reagents; and a measurement chamber to which a sample mixture liquid containing a liquid sample and a solid reagent mixed in the reagent chamber is supplied. The reagent chamber has a form which allows a plurality of the solid reagents to be disposed in the direction substantially parallel to the radial direction of the disc in which centrifugal force is generated, or in the direction substantially perpendicular to the radial direction of the disc.
摘要:
Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.
摘要:
Provided are an automatic analyzing method and apparatus for a microfludic system. The apparatus includes means for recognizing a disc or chip in the microfludic system, performs an operation and detection by automatically loading a DB according to a recognized type, and perform quantitation of a test.
摘要:
This invention relates to an optical disc assembly capable of receiving an insert upon which an analyte of interest may be disposed. The optical disc assembly can be read by an optical disc reader, such as by a standard CD or DVD reader, and the analyte disposed on the insert can be detected by the optical disc reader. The optical disc assembly may have one or more data layer in which operational information or assay information is encoded. Hologram may be used to encode operational information or assay information.
摘要:
A baseline processing device and method are provided for analyzing signals with uneven distributions of pulses and slow varying baselines. In one embodiment, the device includes an A/D sampling unit for sampling a digital counting signal to obtain sampled data, and a baseline extracting unit for sorting the N sampled data in the sampling sequence by magnitude and for outputting, among the N sample data, one sample data A with a value equal to the mid-value in the N sample data. A phase compensating unit with a width of M, to which a digital signal is input, outputs a sampled data B according to a FIFO sequence, wherein M=N/2. A first subtractor subtracts the sample data A from the sample data B and outputs the result as baseline removed data.
摘要:
Provided is a rotatable microfluidic device for conducting simultaneously two or more assays. The device includes a platform which can be rotated, a first unit which is disposed at one portion of the platform and detects a target material from a sample using surface on which a capture probe selectively binds to the target material is attached, and a second unit which is disposed at another portion of the platform and detects a target material included in the sample by a different reaction from the reaction conducted in the first unit.
摘要:
Provided is a microfluidic device and microfluidic system with the device. The microfluidic device includes a substrate; a channel formed in the substrate and in which a fluid can move; a valve controlling flow of a fluid flowing along the channel and including a phase transition material which can be melted by energy such as electromagnetic wave energy; and a lens disposed on the substrate and adjusting an irradiating region of the valve, onto which the energy is applied.
摘要:
Methods and devices for thermal processing of multiple samples at the same time are disclosed. The assemblies include carriers and sample processing devices with process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas. The carriers may be used to apply selective compression to the sample processing devices.
摘要:
A microfluidic device that comprises several microchannel structures in which there are an inlet port, an outlet port and therebetween a substructure comprising a fluidic function. The device has an axis of symmetry around which the microchannel structures are arranged as two or more concentric annular zones. for an inlet port and an outlet port of the same microchannel structure the inlet port is typically closer to the axis of symmetry than the outlet port. Each microchannel structure comprises a substructure that can retain liquid while the disc is spun around the axis and/or the inlet ports are positioned separate from the paths waste liquid leaving open waste outlet ports will follow across the surface of the disc when it is spun. For the microchannel structures of an annular zones the corresponding substructures are at essentially at the same radial distance while corresponding substructures in microchannel structures of different annular zones are at different radial distances. The invention also refers to several other substructures. The substructure are primarily adapted for transporting liquid aliquots that have a surface tnsion >5 mN/m with centrifugal force.