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    Immortalized brain endothelial cells
    71.
    发明授权
    Immortalized brain endothelial cells 有权
    永生化脑内皮细胞

    公开(公告)号：US6093553A

    公开(公告)日：2000-07-25

    申请号：US200063

    申请日：1998-11-25

    Applicant: Mehran Yazdanian ,  Barbara J. Bormann

    Inventor： Mehran Yazdanian ,  Barbara J. Bormann

    IPC: C12N15/09 ,  C07K14/025 ,  C12N5/10 ,  C12Q1/02 ,  C12R1/91 ,  C12Q1/08 ,  C07H21/04 ,  C12N5/00 ,  C12P21/06 ,  C12Q1/68

    CPC classification number: C07K14/005 ,  C12N2710/22022

    Abstract: A brain microvessel endothelial cell having a Middle-T antigen gene from papovirus is disclosed. The brain microvessel endothelial cell exhibits normal cell phenotype, maintains the phenotype in culture and forms monolayers substantially impermeable to low molecular weight molecules. The cells are useful for in vitro studies involving the blood brain barrier. Also disclosed, are methods of using and processes of making these cells.

    Abstract translation: 公开了一种具有来自乳突病毒的中T抗原基因的脑微血管内皮细胞。 脑微血管内皮细胞表现出正常的细胞表型，维持培养中的表型，并形成对低分子量分子基本上不可渗透的单层。 细胞可用于涉及血脑屏障的体外研究。 还公开了使用和制造这些电池的方法。

    Biochemical analysis of antioxidant function of lymphocytes in culture
    73.
    发明授权
    Biochemical analysis of antioxidant function of lymphocytes in culture 失效
    培养中淋巴细胞抗氧化功能的生物化学分析

    公开(公告)号：US5985665A

    公开(公告)日：1999-11-16

    申请号：US665941

    申请日：1996-06-19

    Applicant: J. Fred Crawford ,  Luke Bucci

    Inventor： J. Fred Crawford ,  Luke Bucci

    IPC: C12Q1/08 ,  C12N1/38 ,  C12N5/00 ,  C12N5/02 ,  C12N5/078 ,  C12Q1/00 ,  G01N33/50 ,  A01N1/02 ,  C12Q1/02 ,  G01N1/00

    CPC classification number: C12N5/0634 ,  G01N33/5047 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/95

    Abstract: The present invention provides a cell culture medium useful for a biochemical analysis of antioxidant function in human lymphocytes, said medium comprising, a buffered, serum-free solution containing the following ingredients: a carbohydrate selected from the group consisting of glucose and a compound biologically capable of producing glucose in the cells, a biologically usable form of pantothenic acid, choline or a biological usable form of a substance capable of producing choline in the cells, inorganic ions comprising chloride, phosphate, calcium, magnesium, potassium, sodium, and iron in a biologically utilizable form, cumene hydroperoxide, deionized water, and a mitogen in an amount effective to stimulate the lymphocytes being assayed; said buffered, serum-free solution having a pH from about 6.8 to 7.6, said cell culture medium characterized by being effective to determine nutritional deficiencies, inadequacies, and imbalances and to biochemically analyze antioxidant function of the lymphocytes. Also provided is a method of biochemically analyzing cellular antioxidant function in an individual comprising the steps of: inoculating the cell culture medium of the present invention with lymphocytes from said individual; incubating the inoculated cell culture medium; and comparing the response of the lymphocytes with an average response of lymphocytes from a control group of individuals.

    Abstract translation: 本发明提供了可用于人淋巴细胞抗氧化功能的生物化学分析的细胞培养基，所述培养基包含含有以下成分的缓冲的无血清溶液：选自葡萄糖和生物学能力的化合物的碳水化合物 在细胞中产生葡萄糖，生物可用形式的泛酸，胆碱或生物可用形式的能够在细胞中产生胆碱的物质，包含氯化物，磷酸盐，钙，镁，钾，钠和铁的无机离子 可生物利用的形式，氢过氧化枯烯，去离子水和有效刺激所述淋巴细胞的有丝分裂剂; 所述缓冲的无血清溶液的pH为约6.8至7.6，所述细胞培养基的特征在于有效地确定营养缺乏，不足和不平衡以及生物化学分析淋巴细胞的抗氧化功能。 还提供了一种在个体中生物化学分析细胞抗氧化功能的方法，包括以下步骤：将来自所述个体的淋巴细胞接种本发明的细胞培养基; 培养接种的细胞培养基; 并比较淋巴细胞的反应与来自对照组个体的淋巴细胞的平均反应。

    Method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen
    74.
    发明授权
    Method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen 失效
    确定样品中存在或不存在非贫石棉微生物的方法

    公开(公告)号：US5854013A

    公开(公告)日：1998-12-29

    申请号：US858350

    申请日：1997-05-19

    Applicant: Robert-A. Ollar ,  Mitchell S. Felder

    Inventor： Robert-A. Ollar ,  Mitchell S. Felder

    IPC: C12M1/00 ,  C12M1/24 ,  C12M1/34 ,  C12N1/26 ,  C12Q1/04 ,  C12Q1/08 ,  C12Q1/14 ,  C12Q1/24

    CPC classification number: C12Q1/04 ,  C12M23/08 ,  C12N1/26 ,  C12Q1/14

    Abstract: A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient includes providing a receptacle containing an aqueous solution that does not contain a carbon source and inoculating the aqueous solution with the specimen. A slide having bound thereto a carbon source is placed into the receptacle. By analyzing the slide after exposure to the specimen, the presence or absence of a nonparaffinophilic microorganism in the specimen can be determined. An associated apparatus is also disclosed.

    Abstract translation: 确定从患者取出的样本中存在或不存在非石蜡嗜性微生物的方法包括提供含有不含碳源的水溶液的容器并且将样品接种在水溶液中。 将具有碳源的滑块放入容器中。 通过在暴露于样品后分析载玻片，可以确定样品中存在或不存在非嗜性微生物。 还公开了一种相关装置。

    Silver stain for collagen matrix from paraffin embedded tissue
    75.
    发明授权
    Silver stain for collagen matrix from paraffin embedded tissue 失效
    石蜡包埋组织中胶原基质的银染色

    公开(公告)号：US5700656A

    公开(公告)日：1997-12-23

    申请号：US449910

    申请日：1995-05-25

    Applicant: Si-kwang Liu

    Inventor： Si-kwang Liu

    IPC: G01N1/30 ,  C12Q1/08

    CPC classification number: G01N1/30

    Abstract: A technique for staining and preparing mammalian tissue for examination and study is disclosed. The technique features a fixed tissue that is embedded with paraffin to facilitate the cutting of uniformly thin sections and the penetration of a silver stain into the interstices of the collagen matrix. In particular, a 25-30 micron thick slice of cardiac tissue is silver stained and examined for cardiomyopathies.

    Abstract translation: 公开了一种用于染色和制备哺乳动物组织进行检查和研究的技术。 该技术具有固定的组织，其中嵌入有石蜡，以促进切割均匀的薄部分和银染色渗透到胶原基质的间隙。 特别地，25-30微米厚的心脏组织片被银染色并检查心肌病。

    Precipitate test for microorganisms
    78.
    发明授权
    Precipitate test for microorganisms 失效
    微生物沉淀试验

    公开(公告)号：US5403721A

    公开(公告)日：1995-04-04

    申请号：US51569

    申请日：1993-04-21

    Applicant: N. Robert Ward, Jr. ,  John P. DesRosier ,  Elliott D. Marshall, III ,  Judith Ford ,  Nancy J. S. Mallinak

    Inventor： N. Robert Ward, Jr. ,  John P. DesRosier ,  Elliott D. Marshall, III ,  Judith Ford ,  Nancy J. S. Mallinak

    IPC: C12Q1/04 ,  C12Q1/34 ,  C12R1/19 ,  C12Q1/00 ,  C12Q1/08 ,  C12Q1/10

    CPC classification number: C12Q1/04 ,  C12Q1/34 ,  C12Q2334/52 ,  G01N2333/924 ,  Y10S435/805 ,  Y10S435/97

    Abstract: There is disclosed an improved method for testing for the presence of a particular microorganism or group of microorganisms characterized by a particular enzyme. The method uses a dye-forming substrate throughout a polymer matrix or on the surface of a solid support member that forms a colored precipitate when cleaved by the enzyme. The precipitate is concentrated to the polymer matrix and/or solid support member to create a visible reaction product, wherein the amount of dye-forming substrate needed is independent of the sample size. The present invention further comprises an enzyme indicator device comprising a dye-forming substrate throughout a polymer system or on the surface of a solid support member.

    Abstract translation: 公开了一种用于测试以特定酶为特征的特定微生物或微生物组的存在的改进方法。 该方法在整个聚合物基质中或在固体支持构件的表面上使用染料形成底物，其在被酶切割时形成着色沉淀。 将沉淀物浓缩到聚合物基质和/或固体支持构件以产生可见的反应产物，其中所需的染料形成底物的量不依赖于样品的大小。 本发明还包括酶指示剂装置，其在整个聚合物系统中或在固体支持构件的表面上包含染料形成基质。

    Test device
    80.
    发明授权
    Test device 有权

    公开(公告)号：US11851642B2

    公开(公告)日：2023-12-26

    申请号：US17010467

    申请日：2020-09-02

    Applicant: HITACHI HIGH-TECH CORPORATION

    Inventor： Chihiro Uematsu ,  Muneo Maeshima

    IPC: C12Q1/08 ,  C12Q1/04 ,  G06T19/20 ,  C12M1/34 ,  G06T1/00 ,  C12M1/32 ,  G06T7/00 ,  G02B21/26 ,  G02B21/36

    CPC classification number: C12M41/36 ,  C12M1/34 ,  C12M23/12 ,  C12M41/46 ,  C12Q1/04 ,  C12Q1/08 ,  G06T1/00 ,  G06T7/0016 ,  G02B21/26 ,  G02B21/367 ,  G02B21/368 ,  G06T2207/10056

    Abstract: The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed.

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