公开(公告)号：US20210009483A1

公开(公告)日：2021-01-14

申请号：US17027030

申请日：2020-09-21

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung ,  Kevin Hammill ,  Douglas Higgins ,  Austin Davis-Richardson

IPC分类号： C05F11/08 ,  C12N1/20 ,  C12R1/22 ,  C12R1/025 ,  A01H3/00 ,  C12R1/01 ,  A01N63/00 ,  A01N63/10 ,  A01H6/46 ,  A01H5/06 ,  C12N1/04 ,  C12N15/11 ,  C12N15/52 ,  C12N15/74 ,  C12R1/065 ,  C12R1/07 ,  C12N15/70

摘要： Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.

公开(公告)号：US09783832B2

公开(公告)日：2017-10-10

申请号：US14415343

申请日：2013-07-22

申请人： BIOPOLIS, S.L. ,  UNIVERSIDAD COMPLUTENSE DE MADRID

发明人： Antonia Maria Rojas Martinez ,  Silvia Segarra Manzano ,  Alejandro Montesinos Paes ,  Marta Tortajada Serra ,  Daniel Ramon Vidal ,  Victoria Eugenia Santos Mazorra ,  Miguel Ladero Galan ,  Felix Garcia-Ochoa Soria ,  Vanessa Ripoll Morales ,  Alberto Rodriguez Martín

IPC分类号： C12P7/18 ,  C12R1/00 ,  C12R1/22 ,  C12N1/20 ,  C12N1/32 ,  C12R1/01 ,  C12N15/01

CPC分类号： C12P7/18 ,  C12N1/20 ,  C12N1/32 ,  C12N15/01 ,  C12R1/01

摘要： The invention relates to a method for producing 2,3-butanediol using improved strains of Raoultella planticola, and to novel mutant strains obtained by random mutagenesis from the bacterial species Raoultella planticola CECT843, that can be used in the industrial production of 2,3-butanediol from glycerol. The invention preferably relates to the Raoultella planticola strains designated IA1 and IIIA3 and deposited in the Spanish Type Culture Collection (CECT) under deposit number CECT8158 (corresponding to the strain designated IA1) and deposit number CECT8159 (corresponding to the strain designated IIIA3). The invention also relates to a method for producing 2,3-butanediol from glycerol by means of a biotechnological process using the novel strains of the invention.

公开(公告)号：US09382566B1

公开(公告)日：2016-07-05

申请号：US14041786

申请日：2013-09-30

申请人： The United States of America, as represented by the Secretary of Agriculture

发明人： Cletus P. Kurtzman ,  Neil P. Price ,  Karen J. Ray ,  Tsung Min Kuo

IPC分类号： C12P19/44 ,  C12R1/72 ,  C07H15/10 ,  C07H15/06 ,  C07H15/04 ,  C12R1/22

CPC分类号： C12P19/44 ,  C07H1/00 ,  C07H15/04 ,  C07H15/06 ,  C07H15/10 ,  C12R1/22 ,  C12R1/72

摘要： Open chain sophorolipids may be produced by fermentation with Candida sp. NRRL Y-27208 or C. riodocensis. Dimers and trimers of sophorolipids are also produced. The sophorolipds are produced by inoculating a fermentation medium comprising a carbon source and a lipid, with Candida riodocensis or Candida species NRRL Y-27208, and incubating under aerobic conditions and for a period of time effective to produce an open chain sophorolipid in the medium. The sophorolipids may be subsequently recovered from the fermentation medium.

摘要翻译： 开放链槐糖可以通过用念珠菌sp。 NRRL Y-27208或C. riodocensis。 还生产了槐糖脂的二聚体和三聚体。 通过将含有碳源和脂质的发酵培养基与假丝酵母或假丝酵母属NRRL Y-27208接种，并在需氧条件下孵育一段时间以有效地在培养基中产生开链槐糖脂来制备槐果胶。 随后可以从发酵培养基中回收槐糖脂。

公开(公告)号：US6013494A

公开(公告)日：2000-01-11

申请号：US968563

申请日：1997-11-12

申请人： Charles E. Nakamura ,  Anthony A. Gatenby ,  Amy Kuang-Hua Hsu ,  Richard D. La Reau ,  Sharon L. Haynie ,  Maria Diaz-Torres ,  Donald E. Trimbur ,  Gregory M. Whited ,  Vasantha Nagarajan ,  Mark S. Payne ,  Stephen K. Picataggio ,  Ramesh V. Nair

发明人： Charles E. Nakamura ,  Anthony A. Gatenby ,  Amy Kuang-Hua Hsu ,  Richard D. La Reau ,  Sharon L. Haynie ,  Maria Diaz-Torres ,  Donald E. Trimbur ,  Gregory M. Whited ,  Vasantha Nagarajan ,  Mark S. Payne ,  Stephen K. Picataggio ,  Ramesh V. Nair

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12N15/53 ,  C12P7/18 ,  C12R1/22 ,  C12R1/85 ,  C12R1/865 ,  C12N15/31

CPC分类号： C12N9/0004 ,  C12N15/52 ,  C12N9/16 ,  C12N9/88 ,  C12P7/18 ,  Y10S530/824

摘要： Recombinant organisms are provided comprising genes encoding glycerol-3-phosphate dehydrogenase, glycerol-3-phosphatase, glycerol dehydratase and 1,3-propanediol oxidoreductase activites useful for the production of 1,3-propanediol from a variety of carbon substrates.

摘要翻译： 提供了包含编码甘油-3-磷酸脱氢酶，甘油-3-磷酸酶，甘油脱水酶和1,3-丙二醇氧化还原酶活性的基因的重组生物，其用于从各种碳底物生产1,3-丙二醇。

公开(公告)号：US5601799A

公开(公告)日：1997-02-11

申请号：US369430

申请日：1995-01-06

申请人： Hans Binz ,  Lucien Dussourd D'Hinterland ,  Gerard Normier ,  Alain Le Pape ,  Michel Favaron ,  Souhail Delassan

发明人： Hans Binz ,  Lucien Dussourd D'Hinterland ,  Gerard Normier ,  Alain Le Pape ,  Michel Favaron ,  Souhail Delassan

IPC分类号： A61K9/08 ,  A61K9/12 ,  A61K31/715 ,  A61K49/00 ,  A61K49/16 ,  A61K51/00 ,  A61K51/06 ,  A61P37/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/26 ,  C07K14/41 ,  C08B37/00 ,  C12P19/04 ,  C12R1/22 ,  A61M36/00

CPC分类号： A61K51/06 ,  A61K49/16 ,  C07K14/26 ,  C08B37/006 ,  C12P19/04 ,  A61K2123/00

摘要： The present invention relates to a D 25 polysaccharide compound whose lipid has been partially removed and to its derivatives, wherein at least 85% by weight, preferably 90% of the compound, exists in aqueous solution in monomeric form. The 30 kD molecular weight polysaccharide compound extracted from the membrane proteoglycan of the bacterium Klebsiella pneumoniae is mentioned in particular. The amount of palmitic fatty acids which is bound to it in esterified form does not exceed 0.01% by weight of the compound and the amount of palmitic fatty acids which is associated with it in the free form does not exceed 0.1% by weight of the compound.

摘要翻译： 本发明涉及其部分除去脂质的D 25多糖化合物及其衍生物，其中至少85重量％，优选90％的化合物以单体形式存在于水溶液中。 特别提及从肺炎克雷伯菌细菌的蛋白多糖提取的30kD分子量的多糖化合物。 以酯化形式与其结合的棕榈酸脂肪酸的量不超过化合物的0.01重量％，与游离形式相关联的棕榈酸脂肪酸的量不超过化合物的0.1重量％ 。

公开(公告)号：US5599689A

公开(公告)日：1997-02-04

申请号：US440379

申请日：1995-05-12

申请人： Sharon L. Haynie ,  Lorraine W. Wagner

发明人： Sharon L. Haynie ,  Lorraine W. Wagner

IPC分类号： C12N15/09 ,  C12N15/52 ,  C12P7/18 ,  C12P39/00 ,  C12R1/19 ,  C12R1/22 ,  C12R1/865

CPC分类号： C12P7/18 ,  C12N15/52

摘要： The present invention provides a process for the biotransformation of a carbohydrate carbon source to 1,3-propanediol using mixed yeast and bacterial cultures wherein the carbohydrate is first fermented to glycerol by the yeast cell and then converted to 1,3-propanediol by the bacterial cell containing an active diol or glycerol dehydratase enzyme in this process both the yeast and bacterial cultures are supported on the same carbon source, and 1,3-propanediol is isolated from the media.

摘要翻译： 本发明提供了使用混合酵母和细菌培养物将碳水化合物碳源生物转化为1,3-丙二醇的方法，其中首先通过酵母细胞将碳水化合物发酵成甘油，然后通过细菌将其转化为1,3-丙二醇 在该方法中含有活性二醇或甘油脱水酶的细胞中，酵母和细菌培养物都支持在相同的碳源上，并且从介质中分离出1,3-丙二醇。

公开(公告)号：US5360718A

公开(公告)日：1994-11-01

申请号：US151318

申请日：1993-11-10

申请人： Linda Anderson-Mauser

发明人： Linda Anderson-Mauser

IPC分类号： C12Q1/04 ,  C12Q1/32 ,  C12R1/01 ,  C12R1/22 ,  G01N33/50 ,  G01N33/569 ,  C12N1/00

CPC分类号： C12Q1/32 ,  G01N33/56961 ,  Y10S435/852 ,  Y10S435/921 ,  Y10S436/904

摘要： A method for the presumptive detection of Candida in vaginal fluid, thereby providing an aid to the diagnosis of Candida vaginosis. A sample of vaginal fluid is combined with a test composition comprising an indicator system that provides a chromogenic response in the presence of D-arabinitol; the detection of an elevated level of D-arabinitol indicating the presumptive presence of Candida. The test composition preferably comprises the enzyme D-arabinitol dehydrogenase, NAD, an electron transfer agent, and a chromogenic indicator.

摘要翻译： 一种假阴性检测阴道液中假丝酵母的方法，从而为阴道念珠菌病的诊断提供帮助。 将阴道液的样品与包含在D-阿拉伯糖醇存在下提供显色反应的指示剂系统的测试组合物组合; 检测到D-阿拉伯糖醇水平升高，表明假丝酵母存在推定。 测试组合物优选包含D-阿拉伯糖醇脱氢酶，NAD，电子转移剂和显色指示剂。

公开(公告)号：US5288620A

公开(公告)日：1994-02-22

申请号：US885974

申请日：1992-05-20

申请人： Akinobu Matsuyama ,  Ichiro Takase ,  Yoichiro Ueda ,  Yoshinori Kobayashi

发明人： Akinobu Matsuyama ,  Ichiro Takase ,  Yoichiro Ueda ,  Yoshinori Kobayashi

IPC分类号： C12P7/42 ,  C12R1/01 ,  C12R1/22 ,  C12R1/645

CPC分类号： C12P7/42 ,  Y10S435/822 ,  Y10S435/824 ,  Y10S435/827 ,  Y10S435/83 ,  Y10S435/836 ,  Y10S435/84 ,  Y10S435/843 ,  Y10S435/85 ,  Y10S435/852 ,  Y10S435/853 ,  Y10S435/857 ,  Y10S435/873 ,  Y10S435/874 ,  Y10S435/885 ,  Y10S435/911 ,  Y10S435/921 ,  Y10S435/93

摘要： Optically active 2-hydroxy-4-phenyl-3-butenoic acid can be obtained by treating 2-oxo-4-phenyl-3-butenoic acid with an optionally treated microorganism capable of asymmetrically reducing the 2-oxo-4-phenyl-3-butenoic acid into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid to thereby asymmetrically reduce the same into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid.

摘要翻译： 通过用任意处理的微生物处理2-氧代-4-苯基-3-丁烯酸可以获得光学活性的2-羟基-4-苯基-3-丁烯酸，其能够不对称地还原2-氧代-4-苯基-3-丁烯酸 （R）-2-羟基-4-苯基-3-丁烯酸或（S）-2-羟基-4-苯基-3-丁烯酸，从而将其不对称地还原成（R）-2- 羟基-4-苯基-3-丁烯酸或（S）-2-羟基-4-苯基-3-丁烯酸。

公开(公告)号：US5248608A

公开(公告)日：1993-09-28

申请号：US819129

申请日：1992-01-10

申请人： Theodorus J. G. M. Van Dooren ,  Wilhelmus J. J. Van Den Tweel

发明人： Theodorus J. G. M. Van Dooren ,  Wilhelmus J. J. Van Den Tweel

IPC分类号： C12P13/04 ,  C12P41/00 ,  C12R1/01 ,  C12R1/22

CPC分类号： C12P41/006 ,  C12P13/04 ,  Y10S435/822 ,  Y10S435/824

摘要： Enzyme-catalysed preparation of an .alpha.-substituted optically active carboxylic acid, using enantioselective hydrolysis of a mixture of the enantiomers of the corresponding amide, an Ochrobactrum anthropi or an Klebsiella sp. being used as the enzyme. During the hydrolysis both the L-carboxylic acid and the remaining D-carboxylic acid amide are obtained with high optical purity and high yield, while the activity of the micro-organism is high. A large number of .alpha.-substituted carboxylic acid amides with a remarkable structural variety can be hydrolysed in this manner.

公开(公告)号：US5053499A

公开(公告)日：1991-10-01

申请号：US388806

申请日：1989-08-03

申请人： Eiji Kojima ,  Hidetoshi Yoshioka ,  Hidenori Fukinbara ,  Kunichika Murakami

发明人： Eiji Kojima ,  Hidetoshi Yoshioka ,  Hidenori Fukinbara ,  Kunichika Murakami

IPC分类号： C12P17/18 ,  A61K31/52 ,  A61P31/12 ,  C07D473/00 ,  C07D473/24 ,  C07D473/38 ,  C07D473/40 ,  C07D487/04 ,  C07H19/06 ,  C07H19/12 ,  C07H19/14 ,  C07H19/16 ,  C07H19/173 ,  C12N15/09 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22

CPC分类号： C07H19/06 ,  C07H19/12 ,  C07H19/14 ,  C07H19/16 ,  Y10S435/847 ,  Y10S435/849 ,  Y10S435/852

摘要： 2', 3'-Dideoxy purine nucleosides represented by following general formulae [I] and/or [II] ##STR1## (wherein X and Y indicate nitrogen atoms or carbon atoms and R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 indicate each independently any of hydrogen atom, hydroxyl group, amino group, alkyl group, halogen atom, alkoxy group and mercapto group), process for the preparation thereof and applications thereof to the antiviral agent, antiretroviral agent, therapeutic drug and preventive drug for acquired immunodeficiency syndrome (AIDS), and experimental medicine and experimental reagent to be used in genetic engineering are claimed.

摘要翻译： 2'，3'-二脱氧嘌呤核苷由以下通式[I]和/或[II]表示：其中X和Y表示氮原子或碳原子，R1，R2 ，R3，R4和R5各自独立地表示氢原子，羟基，氨基，烷基，卤原子，烷氧基和巯基中的任一个），其制备方法及其在抗病毒剂，抗逆转录病毒剂，治疗剂 要求用于获得性免疫缺陷综合征（AIDS）的药物和预防药物，以及用于基因工程的实验药物和实验试剂。