公开(公告)号：US07829271B2

公开(公告)日：2010-11-09

申请号：US10576342

申请日：2004-10-21

申请人： Cyril Delattre ,  Gilles Marchand ,  Patrick Pouteau ,  Frédéric Ginot

发明人： Cyril Delattre ,  Gilles Marchand ,  Patrick Pouteau ,  Frédéric Ginot

IPC分类号： C12Q1/00

CPC分类号： B01L3/5088 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L2200/0642 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/0487 ,  C40B40/06 ,  C40B40/10 ,  C40B50/08 ,  C40B60/14 ,  Y10T436/25

摘要： The present invention relates especially to a process for the localized distribution of drops of a liquid of interest on an active surface. The process comprises the following steps: an introduction of liquid of interest into a box containing the said active surface, and an extraction of liquid of interest from the said box, the said active surface and also the other surfaces inside the box being substantially non-wetting with respect to the liquid of interest, with the exception of several uptake areas localized on the said active surface, which are each suitable for taking up a drop of liquid of interest. The uptake areas may surround working areas. The present invention also relates to processes for the electrochemical and optical detection of at least one analyte in a liquid of interest, and to an electropolymerization process.

摘要翻译： 本发明特别涉及一种用于在活性表面上局部分布感兴趣液体的液滴的方法。 该方法包括以下步骤：将感兴趣的液体引入到包含所述活性表面的盒中，以及从所述盒，所述活性表面以及所述盒内的其它表面基本上不相关的萃取液体， 相对于目标液体润湿，除了位于所述活性表面上的几个吸收区域外，其各自适用于吸收一滴感兴趣的液体。 摄取区域可以围绕工作区域。 本发明还涉及用于对感兴趣的液体中的至少一种分析物进行电化学和光学检测的方法以及电聚合方法。

公开(公告)号：US20070207055A1

公开(公告)日：2007-09-06

申请号：US10576345

申请日：2003-10-21

申请人： Gilles Marchand ,  Cyril Delattre ,  Patrick Pouteau ,  Patrice Caillat

发明人： Gilles Marchand ,  Cyril Delattre ,  Patrick Pouteau ,  Patrice Caillat

IPC分类号： G01N21/00

CPC分类号： B01L3/502792 ,  B01J2219/00317 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/0065 ,  B01J2219/00653 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/0487 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

摘要： The present invention relates to an operating device characterized in that it comprises an active surface that is substantially non-wetting with respect to a liquid of interest; at least one zone for the localized capture of a drop of said liquid formed on said active surface; at least one operating zone arranged with a capture zone in such a way that the operating zone is at least partially covered by the drop of the liquid when said drop is captured by said capture zone; and means for leaving a drop of said liquid on said capture zone. This device makes it possible in particular to form high-density arrays of drops of said liquid on a surface, with the aim in particular of carrying out chemical and/or biochemical reactions and/or of analysing the liquid of interest in each drop. It finds, for example, an application in biological chips.

摘要翻译： 本发明涉及一种操作装置，其特征在于其包括相对于感兴趣的液体基本上不润湿的活性表面; 用于局部捕获形成在所述活性表面上的一滴所述液体的至少一个区域; 所述至少一个操作区域布置有捕获区域，使得当所述液滴被所述捕获区域捕获时，所述操作区域至少部分地被所述液滴所覆盖; 以及用于在所述捕获区上留下一滴所述液体的装置。 该装置特别可以在表面上形成所述液体的高密度液滴阵列，其目的是特别是进行化学和/或生物化学反应和/或分析每一滴中感兴趣的液体。 例如，它发现生物芯片中的应用。

公开(公告)号：US20070218452A1

公开(公告)日：2007-09-20

申请号：US10576342

申请日：2004-10-21

申请人： Cyril Delattre ,  Gilles Marchand ,  Patrick Pouteau ,  Frederic Ginot

发明人： Cyril Delattre ,  Gilles Marchand ,  Patrick Pouteau ,  Frederic Ginot

IPC分类号： C12Q1/00 ,  B05D1/30 ,  G01N1/00 ,  G01N21/00

CPC分类号： B01L3/5088 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L2200/0642 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/0487 ,  C40B40/06 ,  C40B40/10 ,  C40B50/08 ,  C40B60/14 ,  Y10T436/25

摘要： The present invention relates especially to a process for the localized distribution of drops of a liquid of interest on an active surface. The process comprises the following steps an introduction of liquid of interest into a box containing the said active surface, and an extraction of liquid of interest from the said box, the said active surface and also the other surfaces inside the box being substantially non-wetting with respect to the liquid of interest, with the exception of several uptake areas localized on the said active surface, which are each suitable for taking up a drop of liquid of interest. The uptake areas may surround working areas. The present invention also relates to processes for the electrochemical and optical detection of at least one analyte in a liquid of interest, and to an electropolymerization process.

摘要翻译： 本发明特别涉及一种用于在活性表面上局部分布感兴趣液体的液滴的方法。 该方法包括以下步骤：将感兴趣的液体引入到包含所述活性表面的盒子中，并从所述盒子，所述活性表面以及盒内的其它表面基本上不润湿提取目标液体 相对于感兴趣的液体，除了位于所述活性表面上的几个摄取区域之外，其各自适合于吸收一滴感兴趣的液体。 摄取区域可以围绕工作区域。 本发明还涉及用于对感兴趣的液体中的至少一种分析物进行电化学和光学检测的方法以及电聚合方法。

公开(公告)号：US20070105210A1

公开(公告)日：2007-05-10

申请号：US10577274

申请日：2004-10-21

申请人： Cyril Delattre ,  Gilles Marchand ,  Patrick Pouteau ,  Frederic Ginot

发明人： Cyril Delattre ,  Gilles Marchand ,  Patrick Pouteau ,  Frederic Ginot

IPC分类号： C12M1/34 ,  H01L21/00

CPC分类号： B01L3/5085 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/0065 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/5088 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/0487 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

摘要： The present invention relates to a work device (1) comprising work zones. It can be used to obtain a matrix of drops on a surface, using a liquid of interest (E). It comprises a work box (Bo) provided with means (o, s) for introducing and extracting the liquid respectively into and from the box; a substrate (S) comprising an active surface that is substantially non-wetting for said liquid of interest contained in said box; distinct work zones (Zt) formed on said active surface and each surrounded by a border (b) formed on said active surface, the borders not touching one another and having no common edge and having a geometry such that when the liquid of interest is extracted from the box, a drop (g) of the liquid of interest remains imprisoned by each border and in contact with the work zone that it surrounds.

摘要翻译： 本发明涉及一种包括工作区的作业装置（1）。 它可用于使用感兴趣的液体（E）获得表面上的液滴基质。 它包括一个设有装置（o，s）的工作箱（Bo），用于分别从盒子中取出和取出液体; 底物（S），其包含对所述盒中包含的所述感兴趣液体基本上不润湿的活性表面; 形成在所述活性表面上并且由形成在所述活性表面上的边界（b）围绕的不同工作区（Zt），所述边界不相互接触并且不具有共同边缘并且具有几何形状，使得当提取所关注的液体时 从盒子里，感兴趣的液体滴（g）仍然被每个边界囚禁，并与其周围的工作区接触。

公开(公告)号：US08215338B2

公开(公告)日：2012-07-10

申请号：US12223059

申请日：2007-02-15

申请人： Cyril Delattre ,  Raymond Charles ,  Patrick Pouteau

发明人： Cyril Delattre ,  Raymond Charles ,  Patrick Pouteau

IPC分类号： A61M5/168

CPC分类号： B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00353 ,  B01J2219/00418 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00689 ,  B01J2219/00698 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/0074 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00862 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00891 ,  B01J2219/0095 ,  B01J2219/00959 ,  B01J2219/00963 ,  B01J2219/00986 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/161 ,  B01L2400/0487 ,  B01L2400/0688 ,  Y10T137/0396 ,  Y10T137/2224 ,  Y10T137/87306 ,  Y10T137/87499

摘要： The method for controlling the progression of a fluid, from upstream to downstream, in a microfluidic component comprising for example a plurality of microchannels each comprising a plurality of successive reaction or detection zones and a plurality of passive valves, controls the progression of the fluid in the microchannels by controlling the increase of a pressure difference between upstream and downstream of the component. The method controls the pressure difference increase discontinuously in the form of enabling pulses so as in particular to synchronize passing of the corresponding passive valves of the microchannels. The pressure difference is advantageously adjusted to a zero value between two successive enabling pulses.

摘要翻译： 用于控制微流体组件中的流体从上游到下游的方法，所述微流体组分包括例如多个微通道，每个微通道包括多个连续的反应或检测区域以及多个被动阀门，控制流体在 通过控制组件的上游和下游之间的压力差的增加来实现微通道。 该方法以可启动脉冲的形式不连续地控制压力差增加，特别是使微通道的相应的被动阀的通过同步。 压力差有利地在两个连续使能脉冲之间调整为零值。

公开(公告)号：US20080314454A1

公开(公告)日：2008-12-25

申请号：US12223059

申请日：2007-02-15

申请人： Cyril Delattre ,  Raymond Charles ,  Patrick Pouteau

发明人： Cyril Delattre ,  Raymond Charles ,  Patrick Pouteau

IPC分类号： G05D7/00

CPC分类号： B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00353 ,  B01J2219/00418 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00689 ,  B01J2219/00698 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/0074 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00862 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00891 ,  B01J2219/0095 ,  B01J2219/00959 ,  B01J2219/00963 ,  B01J2219/00986 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/161 ,  B01L2400/0487 ,  B01L2400/0688 ,  Y10T137/0396 ,  Y10T137/2224 ,  Y10T137/87306 ,  Y10T137/87499

摘要： The method for controlling the progression of a fluid, from upstream to downstream, in a microfluidic component comprising for example a plurality of microchannels each comprising a plurality of successive reaction or detection zones and a plurality of passive valves, controls the progression of the fluid in the microchannels by controlling the increase of a pressure difference between upstream and downstream of the component. The method controls the pressure difference increase discontinuously in the form of enabling pulses so as in particular to synchronize passing of the corresponding passive valves of the microchannels. The pressure difference is advantageously adjusted to a zero value between two successive enabling pulses.

摘要翻译： 用于控制微流体组件中的流体从上游到下游的方法，所述微流体组分包括例如多个微通道，每个微通道包括多个连续的反应或检测区域以及多个被动阀门，控制流体在 通过控制组件的上游和下游之间的压力差的增加来实现微通道。 该方法以可启动脉冲的形式不连续地控制压力差增加，特别是使微通道的相应的被动阀的通过同步。 压力差有利地在两个连续使能脉冲之间调整为零值。

公开(公告)号：US20120064614A1

公开(公告)日：2012-03-15

申请号：US13128783

申请日：2009-10-30

申请人： Patrick Broyer ,  Guillaume Durin ,  Cyril Delattre ,  Frédéric Foucault

发明人： Patrick Broyer ,  Guillaume Durin ,  Cyril Delattre ,  Frédéric Foucault

IPC分类号： C12M1/34 ,  B23P11/00 ,  C12M1/00

CPC分类号： G01N35/1079 ,  B01L3/021 ,  B01L3/50853 ,  B01L3/50855 ,  B01L3/523 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/12 ,  B01L2200/141 ,  B01L2300/044 ,  B01L2300/0829 ,  B01L2300/0848 ,  B01L2300/087 ,  Y10T29/49826

摘要： The invention relates to a device for preparing and/or treating a biological sample including an assembly (2) of storage chambers (3) and/or reaction chambers intended for receiving a fluid and means arranged to move an amount of fluid from and/or to at least one of the chambers (3) of the assembly (2) of chambers (3), the chambers (3) being separated by walls (5) so as to form an assembly of adjacent chambers (3) aligned in a given direction. The means arranged to move an amount of fluid include a needle (6) connected to a transfer space (9), means (8) arranged to enable the suction of a liquid towards a chamber (3) from a transfer space (9) via a needle or delivery from the transfer space (9) to a chamber (3), and driving means (7) arranged to translate the needle (6) and the assembly (2) of chambers relative to one another in the chamber (3) alignment direction, and in that two adjacent chambers (3) are separated by a wall including a sealing membrane (5) or septum capable of being pierced by the needle (6) and of then recovering the seal thereof once the needle is removed. The invention also relates to a method for manufacturing such a device.

摘要翻译： 本发明涉及一种用于制备和/或处理生物样品的装置，其包括储存室（3）和/或用于接收流体的反应室的组件（2）和用于将一定量的流体从和/或 到室（3）的组件（2）的至少一个室（3）中，室（3）被壁（5）分隔，以便形成在给定的对齐中的相邻室（3）的组件 方向。 布置成移动一定量流体的装置包括连接到传送空间（9）的针（6），布置成能够从传送空间（9）经由传送空间（9）从液体朝向腔室（3）抽吸的装置（8） 针或从传送空间（9）传送到室（3）的驱动装置（7），以及驱动装置（7），其布置成在腔室（3）中相对于彼此平移针（6）和腔室组件（2） 并且在两个相邻的室（3）中被包括能够被针（6）刺穿的密封膜（5）或隔膜的壁隔开，然后一旦取出针，就恢复其密封。 本发明还涉及制造这种装置的方法。

公开(公告)号：US20100316531A1

公开(公告)日：2010-12-16

申请号：US12813450

申请日：2010-06-10

申请人： Cyril Delattre ,  Frédéric Bottausci

发明人： Cyril Delattre ,  Frédéric Bottausci

IPC分类号： G01N27/00 ,  B32B37/12

CPC分类号： B01J19/0093 ,  B01J2208/00548 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/161 ,  B01L2400/0427 ,  B29C65/483 ,  B29C65/485 ,  B29C65/4865 ,  B29C65/5042 ,  B29C65/5071 ,  B29C65/521 ,  B29C65/523 ,  B29C65/528 ,  B29C65/8246 ,  B29C66/028 ,  B29C66/1122 ,  B29C66/53461 ,  B29C66/71 ,  B29C66/73161 ,  B29C66/73175 ,  B29L2031/756 ,  B81C1/00 ,  C08J5/12 ,  C09J5/00 ,  B29K2083/00

摘要： A microfluidic device comprising first and second substrates respectively including first and second hydrophobic layers based on polysiloxane, said hydrophobic layers each comprising an assembly area. The substrates are assembled with each other at said assembly areas by means of an adhesive based on silicone.

摘要翻译： 一种微流体装置，包括分别包括基于聚硅氧烷的第一和第二疏水层的第一和第二基底，所述疏水层各自包括组装区域。 基板通过基于硅树脂的粘合剂在所述组装区域彼此组装。

公开(公告)号：US20090053481A1

公开(公告)日：2009-02-26

申请号：US11997477

申请日：2006-08-02

申请人： Guillaume Delapierre ,  Cyril Delattre

发明人： Guillaume Delapierre ,  Cyril Delattre

IPC分类号： B32B3/10 ,  B05D3/10 ,  H01L21/30

CPC分类号： B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  Y10T428/24802

摘要： A method for successively functionalizing a substrate whose surface is provided with at least two areas (1, 2) made up of different materials, with at least one chemical substrate, characterized in that the functionalization is carried out without masking and consists (a) in functionalizing a first area (1) without masking the second area (2) or possible successive areas by transforming the substrate with the aid of a chemical substance X1, wherein the first area material reactivity is greater with respect to the reactivity of the substance X1 than the reactivity of the other possible successive areas with respect thereto, and (b1) in treating the second area (2) or the other possible successive areas with a chemical substance Y1 for removing reaction products deposited on said areas during the functionalization from the areas of the substance X1 and/or the possible successive areas without damaging the functionalized surface of the first area.

摘要翻译： 一种用于使表面设置有由不同材料构成的至少两个区域（1,2）的衬底与至少一个化学衬底相连地功能化的方法，其特征在于，所述功能化在不掩蔽下进行，并且包括（a） 通过借助于化学物质X1转化所述衬底而不掩蔽所述第二区域（2）或可能的连续区域来功能化第一区域（1），其中所述第一区域材料反应性相对于所述物质X1的反应性更大 相对于其它可能的连续区域的反应性，和（b1）在用于除去在功能化过程中沉积在所述区域上的反应产物的化学物质Y1处理第二区域（2）或其它可能的连续区域时 物质X1和/或可能的连续区域，而不损害第一区域的官能化表面。

公开(公告)号：US09285384B2

公开(公告)日：2016-03-15

申请号：US13128783

申请日：2009-10-30

申请人： Patrick Broyer ,  Guillaume Durin ,  Cyril Delattre ,  Frédéric Foucault

发明人： Patrick Broyer ,  Guillaume Durin ,  Cyril Delattre ,  Frédéric Foucault

IPC分类号： G01N35/10 ,  B01L3/00 ,  B01L3/02 ,  B01L7/00

CPC分类号： G01N35/1079 ,  B01L3/021 ,  B01L3/50853 ,  B01L3/50855 ,  B01L3/523 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/12 ,  B01L2200/141 ,  B01L2300/044 ,  B01L2300/0829 ,  B01L2300/0848 ,  B01L2300/087 ,  Y10T29/49826

摘要： The invention relates to a device for preparing and/or treating a biological sample including an assembly (2) of storage chambers (3) and/or reaction chambers intended for receiving a fluid and means arranged to move an amount of fluid from and/or to at least one of the chambers (3) of the assembly (2) of chambers (3), the chambers (3) being separated by walls (5) so as to form an assembly of adjacent chambers (3) aligned in a given direction. The means arranged to move an amount of fluid include a needle (6) connected to a transfer space (9), means (8) arranged to enable the suction of a liquid towards a chamber (3) from a transfer space (9) via a needle or delivery from the transfer space (9) to a chamber (3), and driving means (7) arranged to translate the needle (6) and the assembly (2) of chambers relative to one another in the chamber (3) alignment direction, and in that two adjacent chambers (3) are separated by a wall including a sealing membrane (5) or septum capable of being pierced by the needle (6) and of then recovering the seal thereof once the needle is removed. The invention also relates to a method for manufacturing such a device.

摘要翻译： 本发明涉及一种用于制备和/或处理生物样品的装置，其包括储存室（3）和/或用于接收流体的反应室的组件（2）和用于将一定量的流体从和/或 到室（3）的组件（2）的至少一个室（3）中，室（3）被壁（5）分隔，以便形成在给定的对齐中的相邻室（3）的组件 方向。 布置成移动一定量流体的装置包括连接到传送空间（9）的针（6），布置成能够从传送空间（9）经由传送空间（9）从液体朝向腔室（3）抽吸的装置（8） 针或从传送空间（9）传送到室（3）的驱动装置（7），以及驱动装置（7），其布置成在腔室（3）中相对于彼此平移针（6）和腔室组件（2） 并且在两个相邻的室（3）中被包括能够被针（6）刺穿的密封膜（5）或隔膜的壁隔开，然后一旦取出针，就恢复其密封。 本发明还涉及制造这种装置的方法。