-
1.发明申请USE OF LECITHIN:RETINOL ACYL TRANSFERASE GENE PROMOTER METHYLATION IN EVALUATING THE CANCER STATE OF SUBJECT 有权LECITHIN的使用：用于评估患者癌症状态的催乳素ACYL转移基因促进甲基化公开(公告)号：US20100144867A1
公开(公告)日：2010-06-10
申请号：US12520386
申请日：2007-12-19
申请人： Francis Barany , Yu-Wei Cheng , Philip Paty , Daniel Notterman
发明人： Francis Barany , Yu-Wei Cheng , Philip Paty , Daniel Notterman
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/154 , C12Q2600/16
摘要： The present invention relates to a method of evaluating the cancer state of a subject using lecithin:retinol acyl transferase (LRAT) gene promoter methylation status. Methods of analyzing and quantifying LRAT gene promoter methylation level are also disclosed. The present invention also relates to methods of determining the prognosis for s subject having cancer by assessing LRAT mRNA expression and LRAT protein expression. Methods of cancer detection, diagnosis, prognosis, and treatment are also disclosed.
摘要翻译： 本发明涉及使用卵磷脂：视黄醇酰基转移酶（LRAT）基因启动子甲基化状态评估受试者的癌症状态的方法。 还公开了分析和定量LRAT基因启动子甲基化水平的方法。 本发明还涉及通过评估LRAT mRNA表达和LRAT蛋白表达来测定患有癌症的受试者的预后的方法。 还公开了癌症检测，诊断，预后和治疗方法。
-
2.发明授权Use of lecithin:retinol acyl transferase gene promoter methylation in evaluating the cancer state of subject 有权使用卵磷脂：视黄醇酰基转移酶基因启动子甲基化评估受试者的癌症状态公开(公告)号：US09090941B2
公开(公告)日：2015-07-28
申请号：US12520386
申请日：2007-12-19
申请人： Francis Barany , Yu-Wei Cheng , Philip Paty , Daniel Notterman
发明人： Francis Barany , Yu-Wei Cheng , Philip Paty , Daniel Notterman
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/154 , C12Q2600/16
摘要： The present invention relates to a method of evaluating the cancer state of a subject using lecithin:retinol acyl transferase (LRAT) gene promoter methylation status. Methods of analyzing and quantifying LRAT gene promoter methylation level are also disclosed. The present invention also relates to methods of determining the prognosis for s subject having cancer by assessing LRAT mRNA expression and LRAT protein expression. Methods of cancer detection, diagnosis, prognosis, and treatment are also disclosed.
摘要翻译： 本发明涉及使用卵磷脂：视黄醇酰基转移酶（LRAT）基因启动子甲基化状态评价受试者的癌症状态的方法。 还公开了分析和定量LRAT基因启动子甲基化水平的方法。 本发明还涉及通过评估LRAT mRNA表达和LRAT蛋白表达来测定患有癌症的受试者的预后的方法。 还公开了癌症检测，诊断，预后和治疗方法。
-
3.发明申请METHODS FOR IDENTIFYING GENES WHICH PREDICT DISEASE OUTCOME FOR PATIENTS WITH COLON CANCER 审中-公开识别患有结肠癌患者预防疾病的基因的方法公开(公告)号：US20110257034A1
公开(公告)日：2011-10-20
申请号：US13123689
申请日：2009-10-13
申请人： Francis Barany , Owen Parker , Manny D. Bacolod , Sarah F. Giardina , Yu-wei Cheng , Daniel A. Notterman , Gunter S. Schemmann , Philip B. Paty , Monib Zirvi
发明人： Francis Barany , Owen Parker , Manny D. Bacolod , Sarah F. Giardina , Yu-wei Cheng , Daniel A. Notterman , Gunter S. Schemmann , Philip B. Paty , Monib Zirvi
CPC分类号： G01N33/57419
摘要： Closures for containers and methods for using same are provided. In a general embodiment/the present disclosure provides a closure having a top portion (12), a bottom portion (14) and a side portion (16), an aperture (18) extending though the closure, a projection (20) extending from the closure and at least two rib members (36) on an interior of the projection. The projection may also include a cover (22). In another embodiment, a method for using a closure includes inserting a. spike member into a projection, piercing a membrane that hermetically seals a medical container, pushing rib members within the projection to center the spike member inserted into the projection, and tearing the membrane to create a vent hole in the membrane.
摘要翻译： 提供容器的封闭件和使用容器的方法。 在一般实施例中，本公开提供了一种具有顶部部分（12），底部部分（14）和侧部部分（16）的封闭件，通过封闭件延伸的孔口（18） 所述封闭件和所述突起的内部上的至少两个肋构件（36）。 突起还可以包括盖（22）。 在另一个实施例中，一种使用闭合件的方法包括： 突出部件突入，刺穿密封医疗容器的膜，推动突起内的肋构件以使插入突起中的钉构件居中，并撕裂膜以在隔膜中形成通气孔。
-
公开(公告)号：US07914981B2
公开(公告)日：2011-03-29
申请号：US10854678
申请日：2004-05-25
申请人： Francis Barany , George Barany , Robert P. Hammer , Maria Kempe , Herman Blok , Monib Zirvi
发明人： Francis Barany , George Barany , Robert P. Hammer , Maria Kempe , Herman Blok , Monib Zirvi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , B01J19/0046 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00536 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00729 , B82Y30/00 , C12Q1/6816 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C40B40/06 , C40B60/14 , Y02A50/58 , C12Q2565/501 , C12Q2561/125 , C12Q2537/143 , C12Q2565/514
摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.
-
公开(公告)号：US20070054306A1
公开(公告)日：2007-03-08
申请号：US11590384
申请日：2006-10-31
申请人： Francis Barany , Matthew Lubin , George Barany , Robert Hammer
发明人： Francis Barany , Matthew Lubin , George Barany , Robert Hammer
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
-
6.发明申请Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 有权使用偶联连接酶检测和聚合酶链反应检测核酸序列差异公开(公告)号：US20070048783A1
公开(公告)日：2007-03-01
申请号：US11590056
申请日：2006-10-31
申请人： Francis Barany , Matthew Lubin , George Barany , Robert Hammer
发明人： Francis Barany , Matthew Lubin , George Barany , Robert Hammer
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
摘要翻译： 本发明涉及鉴定靶核苷酸序列的方法。 该方法包括在连接酶检测反应混合物中的靶核苷酸序列上形成连接产物，扩增连接产物以在聚合酶链式反应（PCR）混合物中形成扩增的连接产物，检测扩增的连接产物，并鉴定靶核苷酸 序列。 连接酶检测反应和聚合酶链式反应的这种偶联允许核酸序列差异的多重检测。
-
公开(公告)号：US20050266417A1
公开(公告)日：2005-12-01
申请号：US10939294
申请日：2004-09-10
申请人： Francis Barany , Daniel Turner , Maneesh Pingle , Hanna Pincas
发明人： Francis Barany , Daniel Turner , Maneesh Pingle , Hanna Pincas
IPC分类号： C12N20060101 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6837 , Y02A50/55 , Y02A50/57 , Y02A50/58 , C12Q2561/125 , C12Q2565/102 , C12Q2563/155
摘要： The present invention relates to methods for identifying target nucleic acid molecules differing by one or more single-base changes, insertions, deletions, or translocations; and identifying one or more target mRNA molecules differing by one or more splice site variations in a plurality of mRNA molecules. Also disclosed is a method of generating a linearly amplified representation of a whole genome. Other aspects of the present invention relate to labeled detection oligonucleotide probes and translational oligonucleotide probes as well as to methods of designing such probes.
摘要翻译： 本发明涉及用于鉴定不同于一个或多个单碱基变化，插入，缺失或易位的靶核酸分子的方法; 以及鉴定由多个mRNA分子中的一个或多个剪接位点变异而不同的一个或多个靶mRNA分子。 还公开了一种产生全基因组的线性放大表示的方法。 本发明的其它方面涉及标记的检测寡核苷酸探针和翻译寡核苷酸探针，以及设计这种探针的方法。
-
8.发明申请公开(公告)号：US20050233320A1
公开(公告)日：2005-10-20
申请号：US09963920
申请日：2001-09-26
申请人： Francis Barany , George Barany , Robert Hammer , Maria Kempe , Herman Blok , Monib Zirvi
发明人： Francis Barany , George Barany , Robert Hammer , Maria Kempe , Herman Blok , Monib Zirvi
CPC分类号： C12Q1/6827 , B01J19/0046 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00536 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00729 , B82Y30/00 , C12Q1/6816 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C40B40/06 , C40B60/14 , Y02A50/58 , C12Q2565/501 , C12Q2561/125 , C12Q2537/143 , C12Q2565/514
摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.
摘要翻译： 本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。 该方法包括连接阶段，捕获阶段和检测阶段。 连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。 在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。 捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。 连接阶段之前可以进行扩增过程。 本发明还涉及一种用于实施该方法的试剂盒，在固体支持物上形成阵列的方法，以及载体本身。
-
公开(公告)号：US06949370B1
公开(公告)日：2005-09-27
申请号：US09830502
申请日：1999-10-29
申请人： Francis Barany , Weiguo Cao , Jie Tong
发明人： Francis Barany , Weiguo Cao , Jie Tong
CPC分类号： C12N9/93
摘要： The present invention is directed to a thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.
摘要翻译： 本发明涉及一种具有比T4连接酶或Thermus thermophilus连接酶具有更高保真度的热稳定连接酶。 还公开了编码该酶的DNA分子以及含有它的表达系统和宿主细胞。 本发明的热稳定连接酶可用于进行连接酶检测反应过程和连接酶链反应过程。
-
10.发明授权High fidelity detection of nucleic acid differences by ligase detection reaction 失效通过连接酶检测反应高度保真检测核酸差异公开(公告)号：US06312892B1
公开(公告)日：2001-11-06
申请号：US08891292
申请日：1997-07-10
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C07K14/82 , C12N9/93 , C12Q1/6837 , C12Q1/6862 , C12Q2531/113 , C12Q2561/125 , C12Q2565/501
摘要： Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.
摘要翻译： 利用连接酶检测反应在常规模板过量存在下用热稳定连接酶区分少数模板。 该过程可以用突变连接酶，热稳定连接酶或修饰的寡核苷酸探针进行。 该过程对癌症相关突变的检测特别有用。 它具有提供少量模板数量或比例的定量测量的优点。
-
-
-
-
-
-
-
-
-
搜索结果
70 条记录 (耗时 0.051 秒)