Magnetic device for isolation of cells and biomolecules in a microfluidic environment
    3.
    发明申请
    Magnetic device for isolation of cells and biomolecules in a microfluidic environment 审中-公开
    用于在微流体环境中分离细胞和生物分子的磁性装置

    公开(公告)号:US20050266433A1

    公开(公告)日:2005-12-01

    申请号:US11071679

    申请日:2005-03-03

    CPC分类号: G01N33/54326 B01L3/5027

    摘要: The present invention features a new and useful magnetic device and methods of its use for isolation, enrichment, and purification of cells, proteins, DNA, and other molecules. In general the device includes magnetic regions or obstacles to which magnetic particles can bind. The chemical groups, i.e., capture moieties, on the surface of the magnetic particles may then be used to bind particles, e.g., cells, or molecules of interest from complex samples, and the bound species may then be selectively released for downstream collection or further analysis.

    摘要翻译: 本发明的特征在于一种新的有用的磁性装置及其用于细胞,蛋白质,DNA和其他分子的分离,富集和纯化的方法。 通常,该装置包括磁性颗粒可以结合的磁性区域或障碍物。 然后可以将磁性颗粒表面上的化学基团,即捕获部分用于结合来自复杂样品的颗粒,例如细胞或感兴趣的分子,然后可以选择性地释放结合的物质用于下游收集或进一步 分析。

    Veterinary diagnostic system
    6.
    发明申请
    Veterinary diagnostic system 审中-公开
    兽医诊断系统

    公开(公告)号:US20070059683A1

    公开(公告)日:2007-03-15

    申请号:US11229359

    申请日:2005-09-15

    摘要: The invention relates to a method for diagnosing an animal for a condition by obtaining a fluid sample from the animal, enriching a first analyte having a concentration of less than 1×10−3 analytes/μL from said sample by a factor of at least 10,000 fold; and analyzing one or more enriched first analytes to determine a condition in said animal. Enrichment is preferably performed using one or more size-based separation modules.

    摘要翻译: 本发明涉及一种用于通过从动物获得流体样品来诊断动物的方法,所述方法从所述样品富集浓度低于1×10 -3分析物/μL的第一分析物, 至少10,000倍的因子; 以及分析一种或多种富集的第一分析物以确定所述动物的状况。 富集优选使用一个或多个基于尺寸的分离模块进行。

    Methods and systems for fluid delivery
    8.
    发明申请
    Methods and systems for fluid delivery 审中-公开
    流体输送的方法和系统

    公开(公告)号:US20060121624A1

    公开(公告)日:2006-06-08

    申请号:US11227469

    申请日:2005-09-15

    IPC分类号: B01L3/00

    摘要: The systems and methods herein involve the use of an automated, high-throughput system that utilizes pressure to transfer a fluid medium containing an analyte. In preferred embodiments, the sample is delivered to an analytical device. The sample can comprise one or more analytes, e.g., solvents, solutes, or particles, including rare cells. The systems are designed to minimize contact with potentially hazardous, fragile, or valuable samples. The systems allow for the dilution, mixing, and introduction of the fluid medium to an analytical device, followed by possible further analysis or sample manipulation. The systems and methods herein allow for partial or substantially complete depletion of a sample container to avoid wasting rare analytes or prevent retention of desired material in a first container.

    摘要翻译: 本文的系统和方法涉及使用自动化的高通量系统,其利用压力来传送含有分析物的流体介质。 在优选的实施方案中,样品被递送到分析装置。 样品可以包含一种或多种分析物,例如溶剂,溶质或颗粒,包括稀有细胞。 这些系统旨在最大程度地减少与潜在的危险,易碎或有价值的样品的接触。 该系统允许将流体介质稀释,混合和引入分析装置,随后进行可能的进一步分析或样品操作。 这里的系统和方法允许样品容器的部分或基本上完全的耗尽,以避免浪费罕见的分析物或防止将所需材料保留在第一容器中。

    Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields
    10.
    发明申请
    Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields 有权
    使用与场不对称的障碍物阵列进行连续粒子分离的方法

    公开(公告)号:US20070187250A1

    公开(公告)日:2007-08-16

    申请号:US11640111

    申请日:2006-12-15

    IPC分类号: G01N27/00

    摘要: The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.

    摘要翻译: 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说,本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置,其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中,阵列包括在微流体通道中的有序阵列的障碍物,其中障碍物阵列相对于施加场的方向是不对称的。