公开(公告)号：US20240309425A1

公开(公告)日：2024-09-19

申请号：US18370325

申请日：2023-09-19

Applicant: Fluidigm Corporation

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/686

CPC classification number: C12Q1/6806 ,  B01L3/502738 ,  C12Q1/686 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：US20220413277A1

公开(公告)日：2022-12-29

申请号：US17777894

申请日：2020-11-17

Applicant: Fluidigm Corporation

Inventor： Daaf Sandkuijl ,  Alan Carpino

IPC: G02B21/16 ,  G02B21/32

Abstract: A microscopy apparatus comprises a microscope comprising a stage configured to hold a tissue sample, a UV laser assembly configured to emit a UV laser beam to a viewing area of the tissue sample, and an IR laser assembly configured to emit an IR laser beam to the viewing area of the tissue sample. The UV and IR laser assemblies are oriented so as to emit the respective UV and IR laser beams in a same direction.

公开(公告)号：US11078476B2

公开(公告)日：2021-08-03

申请号：US16105711

申请日：2018-08-20

Applicant: Fluidigm Corporation

Inventor： Jian Qin ,  Peilin Chen ,  Ramesh Ramakrishnan

IPC: C12N15/10

Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

公开(公告)号：US10954560B2

公开(公告)日：2021-03-23

申请号：US16215489

申请日：2018-12-10

Applicant: Fluidigm Corporation

Inventor： Carolyn G. Conant ,  Tze Howe Charn ,  Jason A. A. West ,  Xiaohui Wang

IPC: C12Q1/68 ,  B01L3/00 ,  C12Q1/6874 ,  C12Q1/6844

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

公开(公告)号：US10578633B2

公开(公告)日：2020-03-03

申请号：US14775685

申请日：2014-03-14

Applicant: Fluidigm Corporation

Inventor： Jason A. A. West ,  Brian Fowler

IPC: G01N35/08 ,  G01N33/50 ,  G01N15/10 ,  G01N15/14 ,  C12M1/00 ,  G01N33/569 ,  B01L3/00 ,  C12M1/04

Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

公开(公告)号：US09938641B2

公开(公告)日：2018-04-10

申请号：US11959435

申请日：2007-12-18

Applicant: Jason Andrew Appleton West ,  Brent Coleman Satterfield

Inventor： Jason Andrew Appleton West ,  Brent Coleman Satterfield

IPC: C40B20/00 ,  C40B30/04 ,  C40B20/08 ,  C12N15/115 ,  C40B40/08

CPC classification number: C40B20/08 ,  C07B2200/11 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/10 ,  C40B30/04 ,  C40B40/08

Abstract: Disclosed are methods for performing aptamer preselection based on unique geometry and the content of stems or loops of the aptamer, which methods are capable of providing suitable binders and also permit selection of aptamers performed essentially entirely on a chip or other device. Also disclosed are kits for aptamer selection.

公开(公告)号：US09840732B2

公开(公告)日：2017-12-12

申请号：US13899397

申请日：2013-05-21

Applicant: Fluidigm Corporation

Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

IPC: C12Q1/68 ,  C12P19/34 ,  C12M1/34 ,  C12M3/00 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00 ,  B01D15/08

CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

公开(公告)号：US09663821B2

公开(公告)日：2017-05-30

申请号：US14960754

申请日：2015-12-07

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

IPC: G01N21/00 ,  G01N15/06 ,  C12Q1/68 ,  G01N21/64 ,  G01N27/447 ,  G02B21/16 ,  G01N21/84 ,  G01N21/75 ,  G02B21/36 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

公开(公告)号：US09579830B2

公开(公告)日：2017-02-28

申请号：US13813625

申请日：2009-07-23

Applicant: David S. Cohen

Inventor： David S. Cohen

IPC: B01L3/00 ,  B32B37/18 ,  B32B38/00 ,  B29C41/02 ,  G01N30/60 ,  B29K83/00 ,  B29L9/00 ,  B29L31/00

CPC classification number: B29C41/02 ,  B01L3/502 ,  B01L3/502707 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B29K2083/00 ,  B29L2009/00 ,  B29L2031/755 ,  B32B37/182 ,  B32B38/0008 ,  B32B2309/105 ,  B32B2383/00 ,  G01N30/6095

Abstract: An integrated fluidic chip includes a substrate defined by a lateral surface area greater than 28 square inches. The integrated fluidic chip also includes a first elastomeric layer having a mold surface and a top surface. The mold surface of the first elastomeric layer is joined to a portion of the substrate. The first elastomeric layer includes a plurality of first channels extending normally from the substrate to a first dimension inside the first elastomeric layer. The integrated fluidic chip further includes a second elastomeric layer having a mold surface and a top surface. The mold surface of the second elastomeric layer is joined to at least a portion of the top surface of the first elastomeric layer.

Abstract translation: 集成流体芯片包括由大于28平方英寸的侧表面积限定的基板。 集成流体芯片还包括具有模具表面和顶表面的第一弹性体层。 第一弹性体层的模具表面连接到基底的一部分。 第一弹性体层包括多个第一通道，其从基底正常延伸到第一弹性体层内的第一尺寸。 集成流体芯片还包括具有模具表面和顶表面的第二弹性体层。 第二弹性体层的模具表面连接到第一弹性体层的顶表面的至少一部分。

公开(公告)号：US09316331B2

公开(公告)日：2016-04-19

申请号：US14091342

申请日：2013-11-27

Applicant: Fluidigm Corporation

Inventor： Geoffrey Facer ,  Brian Fowler ,  Emerson Cheung Quan ,  Marc Unger

IPC: B01L3/00 ,  G05D11/02 ,  F16K99/00 ,  B01F5/02 ,  B01F13/00 ,  C12Q1/68 ,  G01N21/75 ,  F16K3/00 ,  G01N21/03

CPC classification number: F16K99/0015 ,  B01F5/02 ,  B01F13/0059 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  B33Y80/00 ,  C12Q1/686 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N2021/0346 ,  Y10T137/0329 ,  Y10T137/2164 ,  Y10T137/2169 ,  Y10T137/2559 ,  Y10T137/2655 ,  Y10T137/2688 ,  Y10T137/87249

Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.