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公开(公告)号：US20230372935A1
公开(公告)日：2023-11-23
申请号：US18362530
申请日：2023-07-31
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz , Erin R. Chia , Billy W. Colston , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
CPC classification number: B01L3/50273 , B01F23/41 , B01F33/813 , B01F33/3011 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/23
Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
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公开(公告)号：US09822393B2
公开(公告)日：2017-11-21
申请号：US14201752
申请日：2014-03-07
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
CPC classification number: C12Q1/6804 , B01L3/502784 , B01L7/52 , C12Q1/6844 , C12Q1/6851 , C12Q2527/125 , C12Q2563/159 , C12Q2537/143
Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
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公开(公告)号：US09758535B2
公开(公告)日：2017-09-12
申请号：US14216006
申请日：2014-03-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
CPC classification number: C07F7/1804 , C08G77/46 , C08G77/50 , C08L83/12 , C08L83/14 , G01N33/5436
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
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公开(公告)号：US20170056884A1
公开(公告)日：2017-03-02
申请号：US15351331
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods, apparatus, and kits, for forming and concentrating emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, a droplet well, and a channel network interconnecting the wells. The system also may comprise an instrument configured to operatively receive the device and to create (i) a first pressure differential to produce an emulsion collected in the droplet well and (ii) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well.
Abstract translation: 用于形成和浓缩乳液的系统，包括方法，装置和试剂盒。 一个示例性系统可以包括一个装置，该装置包括一个样本阱，其被配置成接收含有样品的流体，一个连续相阱，被配置成接收连续相流体，一个液滴阱以及一个使阱连接的通道网络。 该系统还可以包括被配置为可操作地接收该装置并且产生（i）第一压差以产生在液滴阱中收集的乳液的仪器，和（ii）第二压力差以减小连续相流体的体积分数 在乳液中，在乳液已经收集在液滴中之后，通过相对于含有样品的液滴从液滴中选择性驱动连续相流体。
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公开(公告)号：US20140200164A1
公开(公告)日：2014-07-17
申请号：US14159410
申请日：2014-01-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Anthony J. Makarewicz, JR. , Amy L. Hiddessen , Kevin D. Ness , Donald. A. Masquelier , Billy W. Colston, JR. , Benjamin J. Hindson , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
IPC: C12Q1/68
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods and apparatus, for detection of spaced droplets.
Abstract translation: 用于检测间隔液滴的系统，包括方法和装置。
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Patent Agency Ranking
公开(公告)号：US10676778B2
公开(公告)日：2020-06-09
申请号：US15818601
申请日：2017-11-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
IPC: C12Q1/68 , C12Q1/6804 , B01L7/00 , C12Q1/6844 , C12Q1/6851 , B01L3/00
Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
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公开(公告)号：US20180147573A1
公开(公告)日：2018-05-31
申请号：US15707908
申请日：2017-09-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, Jr. , Erin R. Chia , Billy W. Colston, Jr. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
IPC: B01L3/00 , B01L9/00 , B01F13/00 , C12Q1/686 , B01F13/10 , B01F3/08 , B01F5/02 , B01F5/00 , G01N35/08
CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.
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公开(公告)号：US20170152550A1
公开(公告)日：2017-06-01
申请号：US15389116
申请日：2016-12-22
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Erin R. Chia
CPC classification number: C12Q1/6816 , B01F5/0085 , B01F5/0647 , B01F9/00 , B01F11/00 , B01F13/0062 , B01F13/0072 , B01F2215/0037 , B01L3/502784 , B01L3/52 , B01L2200/148 , B01L2200/185 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , C12Q1/6806 , G01N1/38 , G01N21/6428 , G01N21/645 , G01N2001/2893 , G01N2021/6439 , G01N2201/127 , C12Q2563/159
Abstract: Method of detecting a signal from droplets for instrument calibration. In the method, a calibration standard for a droplet detection instrument may be received. The calibration standard may have been shipped at least one kilometer when received. The calibration standard may include a mixture of first droplets and second droplets. Each of the first and second droplets may be encapsulated by an immiscible carrier liquid and may have a stabilizing droplet skin that is formed by heating and that includes a skin-forming protein. Each the first droplets may contain a fluorescent dye, and each of the second droplets may contain a fluorescent dye. A fluorescence signal may be detected from a plurality of the first and second droplets with a droplet detection instrument. The fluorescence signal may be stronger for the first droplets than the second droplets.
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公开(公告)号：US09492797B2
公开(公告)日：2016-11-15
申请号：US14159410
申请日：2014-01-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Anthony J. Makarewicz , Amy L. Hiddessen
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: Method of detection for droplet-based assays. In an exemplary method, an open end of a channel network may be placed into an emulsion. Droplets of the emulsion may be driven along a flow path from the open end, through a confluence region where a dilution fluid is introduced into the flow path from at least one dilution inlet channel to increase an average distance between droplets, and through an examination region disposed downstream of the confluence region. Light may be detected from the examination region as droplets pass through. The channel network may include a droplet inlet channel that meets the at least one dilution inlet channel at the confluence region. The droplet inlet channel may form a tapered region that is sized such that droplets leave the tapered region in single file.
Abstract translation: 基于液滴的检测方法。 在示例性方法中，通道网络的开放端可以被放置在乳液中。 乳液的液滴可以沿着来自开口端的流动路径被驱动通过汇合区域，其中稀释流体从至少一个稀释入口通道引入到流动路径中，以增加液滴之间的平均距离，并且通过检查区域 位于汇合区域的下游。 当液滴通过时，可以从检查区域检测光。 通道网络可以包括在汇合区域处与至少一个稀释入口通道相遇的液滴入口通道。 液滴入口通道可以形成锥形区域，该锥形区域的尺寸使得液滴在单个文件中离开锥形区域。
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公开(公告)号：US20140170665A1
公开(公告)日：2014-06-19
申请号：US14018205
申请日：2013-09-04
Applicant: BIO-RAD LABORATORIES, INC.
Inventor： Amy L. Hiddessen , Erin Steenblock
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2300/0816 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2400/0487
Abstract: This disclosure provides systems, devices, and methods for sample preparation and/or analysis comprising a droplet generator having a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel may meet at an intersection that receives a sample from the sample reservoir and a carrier fluid from the carrier fluid reservoir and generates one or more droplets that flow along a droplet channel to a droplet reservoir. An energy application member in thermal communication with the intersection, at least a portion of the droplet channel, the sample reservoir and/or the carrier fluid reservoir may provide energy to an individual droplet of the one or more droplets, such as at the intersection and/or as the droplet moves along the droplet channel to the droplet reservoir.
Abstract translation: 本公开提供了用于样品制备和/或分析的系统，装置和方法，其包括具有与载体流体储存器流体连通的第一通道和与样品储存器流体连通的第二通道的液滴发生器。 第一通道和第二通道可以在从样品储存器接收样品和来自载体流体储存器的载体流体的交叉点处相遇，并且产生沿着液滴通道流动到液滴储存器的一个或多个液滴。 与交叉点热连通的能量施加构件，液滴通道，样品储存器和/或载体流体储存器的至少一部分可以向一个或多个液滴的单个液滴（例如在交叉点处）提供能量，并且 /或当液滴沿着液滴通道移动到液滴储存器时。
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